한국동물학회:학술대회논문집 (Proceedings of the Zoological Society Korea Conference) (Proceedings of the Zoological Society Korea Conference)
한국통합생물학회 (The Korean Society for Integrative Biology)
- 연간
과학기술표준분류
- 농림수산식품 > 동물자원과학
한국동물학회 1995년도 한국생물과학협회 학술발표대회
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Glycoprotein Hormones (LH/CG, FSH and TSH) and their Receptors: Interaction and Receptor Activation.No Abstract, See Full Text
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Regulation of enzyme synthesis by transcriptional and translational control systems provides rather stable adaptation to change of amino acid level in the growth medium, while manipulation of enzyme activity through endproduct feedback inhibition represents rather short-term and reversible ways of adjusting metabolic fluctuation of amino acid level. Various control mechanisms interplay to regulate genes encoding enzymes for amino acid biosynthesis in the yeast, Sacchromyces cerevisiae. When amino acids are in short supply, genes under a cross-pathway regulatory mechanism Or general amino acid control (general control) increase their action, in which Gcn4p is the major positive regulator of gene expression. When cells are cultured in minimal medium, basal level expression is also regulated by supplementary control elements, where inorganic phosphate level is additionally involved. Most of amino acid biosynthetic genes are also regulated by the level of endproduct of the pathway. This pathway-specific regulatory mechanism is called specific amino acid control (specific controD, under which gene expression is reduced when endproduct is present in the medium. Derepression of a gene through general control can be usually overridden by repression through specific control, where the endproduct level of that particular pathway is high and not limiting. In this presentation, regulatory factors for basal level expression and general control of yeast amino acid biosynthesis will be discussed, m addition to pathway-specific repression patterns and interaction between CrOSS- and specific-control mechanisms. Preliminary results are also presented from the investigation of the cloned genes in the threonine biosynthetic pathway of the yeast. yeast.
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The three chitin synthetases of Saccharomyces cerevisiae, Chs1, Chs2, and Chs3, participate in septum and cell wall formation of vegetative cells and in wall morphogenesis of conjugating cells and spores. Because of the differences in the nature and in the time of execution of their functions, the synthetases must be specifically and individually regulated. The nature of that regulation has been investigated by measuring changes in the levels of the three synthetases and of the messages of the three corresponding gnes, CDSI, CHS2, and CAL1/CSD2/DITl0l(referred to below as CAL1), during the budding cycles. For Chs1 and Chs3, posttranslational regulation, probably by activation of latent forms, appears to be predominant. Since Chs2, like Chs1, is found in the cell in the zymogenic form, a posttranslational activation step appears to be necessary for this synthetase also. The regulation mechanism was investigated to search the relationship of CAL1, CAL2 and CALJ which is involved in Chs3 activity us ing different assay methods other than previous one. Treatment of Chs3-containing membranes with detergents drastically reduced the enzymatic activity. Activity could, however, be restored by subsequent incubation with trypsin or other pro teases in the presence of UDPGlcNAc. Experiments wi th mutants in the three genes invoIved in Chs3 activity-CAL1, CAL2, and CALJ-showed that only CAL1 and CALJ are required for the proteaseelicited (zymogenic) activity. It is concluded that Chs3 IS a zymogen and that the CAL2 product funct ions as its activator.ivator.
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Microorganism are centIal core to biosphere sm1ainablity and biogeochemical cycles on this earth. Most of food, medical and pabamceutical new materials through biotechnology are derived from many kinds of microorganisms. Microorganisms are important resources of biotechnology. Beside these, micorbial diversity is key to explore the frontiers of knowledge about the strategies and limits of life. Through the micorotganisms, we can monitor the environmental changes and conditions. Moreover, the microorganismsms play a role in conservation and restoration of higher plants and animals. And we can get a lot of ecological, evolutionary knowledges from microbial models. In spite of these importances, the microbial diversity is not properly evaluted because of their unculturablity. Only 0.001 - 3 % of total bacteria in natural habitats are cultumble and the rest are viable but uncultumble. Only 3,100 species are listed up in the Bergey's Manual. Considering the symbisis and estimated numbers of insect are more than 800,000, the symbiotic microorganisms are about 1,000,000 species. Recently, by using the genetic and molecular technics, the microbial diversity is now unveiled. In this symposium, the genetic, species and ecological diversity will be given. given.
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The total size of the pF AR1, a genomic clone of Frankia FaCI, was estimated to be about 44Kb by summation of the individual fragment length generated by single or double restriction enzymes. Southern hybridization analyses with Azotobacter vinelandii nif-genes as probes and partial sequencing analyses of the subclones revealed that organization of the nif-gene in the FaCI strain was nifV, H, D, K, E, N, X, W, B. The organization of the structural genes for nitrogenase is the same in this Frankia strain as it is in most other nitrogen-fixing prokaryotes but the positioning of the nifV-like gene relative to the nifHDK cluster differs. A consensus nif-promoter-like sequence, found at 5' of nifH, was not detected upstream of the niJV-like gene. nifV-like gene contained a ORF of 1206 NT encoding 401 amino acids. The nucleotide sequence and deduced amino acid sequence of the gene exhibit homology value of 65% and 41% with that from A vinelandii, respectively. The putative Shine-Dargamo sequences were present preceding nitK, nifH, D, K, and nifE, and in nitK gene putative start codon GTG was detected instead of A TG. The nucleotide and amino acid sequence of niIK of FaCI showed 82% and 76% homolgy with those of Frankia HFPCc 13, respectively. Amino acid sequence of niIK showed 69% and 61% homology with those of A vinelandii, Klebsiella pnewnoniae, respectively, while that of nifE 73% and 71%, respecti vely.i vely.
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Insulin Synthesis and Secretion in Human
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Study on The Susceptibility of MRSA to Fluoro-quinolones and Relation between Structure and ActivityNo Abstract, See Full Text
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Purification and Characterization of an Acetate specific Esterase from Streptomyces coelicolor A3(2)No Abstract, See Full Text
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Kim, Woo-Ka-P;Kim, Eun-Young;Kang, Seong-Hoon;Yeun, Tai-Wook;Lee, Jia-Jeong;Hyun, Dong-Hoon;Kwon, Oh-Oak;Nam, Myoung-Hee;Chai, Kweon-Seok 307.2
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Analysis on Genes Related to Antituberculosis Drugs by Single Strand Conformation Polymorphism(SSCP)No Abstract, See Full Text
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