Allergy has a born predominant cause of atopy in body and react oversenstively several meterial. The cause of allergy are tick, house dust, egg, milk, bean, cosmetics, virus, bacteria, flesh and meat in Western medicine. While, Oriental medicine did not have an application on the allergy, but recognized that allergy is connected with fetal toxicosis, heat-syndrome in new born, syndrome characterized by dyspnea, sneezing, stuffy nose, nasal discharge and phlegm. Therefor, We knowed method of medical treatment following cause of disease and pathogenesis against allergy. Allergic disease(atopic dermatitis, bronchial asthma, rhinitis) were related with lung, spleen, kidney. To prevent allergic disease in born, Oriental medicine taked a serious view of fetal education. In Western medicine, The cause of allergy applied to exopathogen of Oriental medicine. In Oriental medicine, Treatment of allergy used generally strengthening the body resistance to eliminate pathogenic factors.

The application of Shinkihuan umder the combination of configuration, color, pulse and symptoms leads to the following conclusions: Shinkihuan is applied to the diseases related with lung, kidney and the spine: difficult urination of old people, shortness of breath, hematuria, loss of voice, distorted vision, deafness of deficiency, hemorrhoids, thirst, carbunde, suppurative infection, children's retardation of walking and noma, etc ... The effect of Shinkihuan is as follows. It nourishes the lung, replenishes the kidney fluid, relieves the phlegm, tranquilizes fever due to deficiency of blood and reinforces the liver. Shinkihuan is appropriate for the persons with the following characteristics in configuration: male, tall, thin, dam-typed, inverted-triangular, prominent nosed and dry.

This paper suggest a methodology for representation of findings which can be called as signs and symptoms. A finding consists of unit signs and unit symptoms, and moreover findings which appear in one individual patient have so many different relationship each other. So, it is nat appropriate to list all of possible findings as medical standard or to fill findings as independent things in paper for medical record. We try to distinguish finding item from finding list, and suggest the methodology by which we can make finding list from finding items. That is, we suggest finding item[Concept], value types, relationship, logical operator, and syntax as a component of representation. And by using urinary symptom, we make the example for representation methodology. Finally, we mention the background knowledge, brief research process of related area.

All creatures are living in the space and time. As the space and time are prior to experience, they are preconditions for an incident to happen and preconditions for each other to coexist as well. Therefore, time can be recognized through the change of space and the space can be understood by the passage of time. In western philosophy, the space was understood as an object, place, interval, mind and etc. In oriental philosophy, even though one space is just a part of bigger space, the space may represent the universal space, and the various spaces are no more than a space. The space itself doesn't have any color, form, beginning and end, or liu-he(六合). However, it is the biggest concept that we can find everywhere. In order to understand the space, we need to find our position by expressing subjective positions like above and below, left and right, before and after, and objective positions like high and low, east and west, south and north. In oriental philosophy, the sun is the standard point in finding position; its front side is south, the backside is north, the left side is east, the right side is west, the upper side is south and the lower side north. Based on the finding position which is stated above and by taking each characteristics of he-luo-xi-wen(河洛羲文) and interrelations among them, the space can be modeled. Followings are the results obtained from this study: Tian doesn't fill in west and north. Di doesn't fill in east and south. Tian-dao(天道) turns to left, and Di-dao(地道) turns to right. There is no direct way to get to Dui-chong-fang without passing by Zhong-gong(中宮). The solid figure of eighty-one Bian-ju(變局) and sixty-four Gua-tu(卦圖).

The main current in the pathology of oriental medicine is composed of etiologic factor, pathogenesis and clinical manifestation. The access of a disease is consist of etiologic factor, location of the lesion, nature of the disease and patient's condition. The nature of disease and the property of a drug are inseparably related to each other. The nature of disease is composed of six exogenous factors, cold and heat, deficiendy and excess, Yinyang and pain. Cold nature is divided into cold symptom due to excess and asthenia cold, fever nature is divided into sthenic fever and asthenic fever. According to the location, cold and heat can be subdivided into heat in the upper and cold in the lower, cold in the upper and heat in the lower, exterior cold and interior heat, exterior heat and interior cold. Yin syndrome characterized by hypofunction of the viscera is generated from insufficiency of yang-qi, excess of yin-coldness, deficiency of both qi and the blood.

Traditional oriental medicine and western medicine have not only their own views of disease but also their own practical approach to disease. So, We should not identify the thing which is expressed as ‘ai’(癌) in traditional oriental medicine with the thing which is expressed as ‘cancer’ in western medicine. In traditional oriental medicine, the name of malignant tumor is also designated by it's sign and symptom as same as different disease. So it is difficult to compare directly ‘ai’(癌) with cancer. However, when we analysed processes and characteristics of disease, we can find many instances which is related to the things which is called as cancer by western medicine. So at first, we analysed the meaning of word, ai(癌). Secondly, through the searching many instances which are mentioned above, we classified four groups and investigated these. Four group is following like, First, the cases that ai(癌) is used in. Second, the case that is recognized as mass. Third, the case that has different shape with ai. Fourth, the case that has similar sign and symptom with cancer.

We study on the significance and application of the whole body form diagnosis. The results were as follows; The general form diagnosis is the method to observe the individual physiology and pathology. The phase of thinking, the current and activity of KI, the pattern of general form diagnosis have organic relations with the symptoms. The general form diagnosis is made up the principle of the imaging phase, therefore it must make synthetic union the differentiation of syndromes. The general form diagnosis of NAE GYEONG shows the typical phases and it is divided with the sight of YIN YANG and Five-Element. The general form diagnosis of SEOP GAE is practiced the theory of constitution's demonstration before the understanding of symptoms. Then JANG NAM tried the type of constitution's demonstration. The general form diagnosis of DONG MU becomes the diagnostic root of constitution's demonstration in four type constitution theory.

This treatise is written in order to solve the important contradiction between the two theories; in oriental medicine psychological function is responsible for heart, but in western one it is responsible for brain. So we take the methods of studying in the aspects of morphological characteristics(MC) and visceral manifestation theory(VMT, 藏象論) and others about two organs-heart and brain. Brain(頭腦) is preferred to understand as a structure which is manifesting mental activity of heart. So the brain can be named with external heart(外心) corresponding to the relation of kidney(外 and external kidney. Saying conversely, the nutritional foundation of the mental function is the blood of heart, but the enlightening and insightful features of mentality make it's own residence move to the organ in the uppermost and positive site, that is head. And the close relationships on mental functions between heart and brain were discussed in various aspects, like investigation on east and west etymological literature, or Jiu gong and Taoist theory as well as Me and VMT, These understandings can make us know about the pathology of brain by itself. It has deep relations with heart fire and heart blood and kidney essence, and gastrointestinal function and liver with lung additionally. In another point, it makes the highly complicated psychological functions to be explained free from body relatively, and so can do a role in the complement of the strict 5 viscera theory.

Nae-Kyung says the puberty is corresponded to the age of 16～24(male) and 14～21 (female). And that time they are promoted the growth. However, Kidney qi doesn't make average situation during that time. So, the function of five viscera and six entrails fails to be perfect and symptoms appear. I came to the conclusion through the survey of about 200 high school students(male and female) and the theory of oriental medicine the distinctive features (like migraine, amnesia, depression, dysphoria, inappetence, irregular menstruation) came out that time. I defined this distinctive symptoms in boys and girls at puberty ‘synthetic symptoms of puberty’. For the medical cure and prevention I prescribed ‘Sachun-Nyung’ and that has medical benefits for ‘Replenishing qi and relieving the spleen, Soothing the liver and promoting blood circulation’

Citri Reticulatae Viride Pericarpium extract(CRVP) have been used in oriental medicine for many centuries as a therapeutic agent for smoothing the liver and regulating the circulation of qi, and promoting digestion and removing stagnated food. The effects of CRVP on the inhibition of brain damage in cerebral ischemia is not known. Therefore, this Study was designed to investigate the cerebral protective effects of CRVP on the transient cerebral ischemia using modern techniques, and further to provide the possibility of scientification of oriental medicine. The size of cerebral infarct size was measured by morphometry, and brain edema was measured by morphometry and brain water content determination. The results were a$ follows ; 1. Water fraction of CRVP was reduced infect area of rats brain slices which were subjected to a transient cerebral ischemia in a dose-dependent manner. 2. Methylene chloride fraction and hexane fraction of CRVP was significantly reduced infarct area of rats brain slices which were subjected to a transient cerebral ischemia in a dose-dependent manner. 3. Methylene chloride fraction and hexane fraction of CRVP was significantly reduced infarct volume of rats brain which was subjected to a transient cerebral ischemia in a dose-dependent manner. 4. Methylene Chloride fraction and hexane fraction of CRVP was significantly decreased brain edema induced by a transient cerebral ischemia in a dose-dependent manner. 5. Methylene chloride fraction and hexane fraction of CRVP was significantly decreased brain water content of rats which were subjected to a transient cerebral ischemia. It is suggested that CRVP has an anti-ischemic effect through the inhibition of brain damage in a transient cerebral ischemia, and that in future further development of main effective constituent in CRVP can provide a novel therapeutic strategy for cerebral ischemia.

For determination of the genotoxicity of VOCs(Volatile Organic Compounds) in water, in vitro Comet assay was performed using 3T3 cells. The selected 5 VOCs; Trichloroethylene(TCE), Tetrachloroethylene(PCE), Carbontetrachloride (CteC), Dichloromethane(DCM) and Chlorofrom(Chl) and mixed solvent(Mix), are the test items for drinking water quality. Author analyzed the genotoxicity of these solvents through their tail length (TL) values. Mix, PCE, Chl, TCE in order had cytotoxicity at the highest concentration, and CCl₄ and DCM had no cytotoxic effect. TCE, CCl₄, Chl, PCE, Mix, DCM had genotoxicity, Chl, PCE, Mix had both cytotoxicity and genotoxicity simultaneously, Cytotoxic effect of mixed organic solvents, compared with that of single component, at each concentration, was influenced by the synergistic effect of the interaction of each organic component.

To investigate an eliminatory effect of mixed extract of Hovenia dulcis Thunb, Gastrodia elata and Alnus japonica etc., on the ethanol-induced hangover, 12 hr-fasted male Sprague-Dawley rats weighing 220±20 g were given mixed extract (10 mL/kg, p.o.) and administered ethanol at a dose of 3 g/kg bw (25% in distilled water) orally 30 min postdosing. Blood was collected from caudal artery at 0.25, 0.5, 1, 2, 4, 6, 8, 10, and 12 hr and then the animals were sacrificed at 24 hr after the ethanol treatment. From 0 to 12 hr, the administration of mixed extract significantly decreased the area under the serum alcohol concentrations-vs.-time curves by 21 % compared with control group. In these experiments, liver function indices, such as alanine aminotransferase, aspartate aminotransferase and sorbitol dehydrogenase activities, showed unaltered results in all treated groups compared with the normal group. These results suggest that oral intake of the mixed extract may be effective on elimination of ethanol-induced hangover.

Rubi Fructus (fruit of Rubus coreanus Miq.), oriental medicine, has been used for remedy of the liver diseases and for tonic. In order to investigate the oxygen free radical, a harmful factor of aging, in liver of rats fed diets supplemented with the Rubi Fructus water extract(RX), Sprague-Dawley male rats have been fed a diet supplemented with 3% or 5% RX for a month. In rats fed 3% RX supplemented diet, hepatic cytochrome P-450 contents appeared to be increased, and catalase and superoxide dismutase activities were significantly increased compared with the control. There was no difference in glutathione peroxide and glutathione-S-transferase activities between the rats fed RX supplemented diets and the control diet. In conclusion, it is likely that rats fed a diet supplemented with RX may have the oxygen free radicals detoxication potential.

This experiment is carried out for test whether the addition temperament drugs of Danchisoyo-san have an anti-inflammatory effect and have suppression effect on immunocyte in the state of inflammation which induced by carageenan and zymosan. The freezing lyophilization powder which were extracted from Danchisoyo-san divided low dose group(200mg/kg-DSL) and high dose group(500mg/kg-DSH) and after melting in water, it was orally administered to the mouse. Compared with inflammation induced group which were induced by triggering-inflammation reagent carageenan and zymosan and normal contrast group, we measured the edema decrement effect, macrophage and spleen cell activation. 1. Danchisoyo-san has suppress inflammatory reaction induced by carrageenan. 2. Danchisoyo-san has suppress increasing activation of abdominal cavity macrophage cell in the carrageenan induced inflammation. 3. Danchisoyo-san has suppress increasing activation of spleen cell in the carrageenan induced inflammation. 4. Danchisoyo-san has suppress increasing activation of abdominal cavity macrophage in the zymosan induced inflammation. 5. Danchisoyo-san has suppress increasing activation of spleen cell in the zymosan induced inflammation. Based on the above result, Danchisoyo-san was improved its suppression effect to the inflammatory reaction through the suppression of spleen cell and macrophage activation. So we concluded that Danchisoyo-san is prospected as a anti-inflammatory agent to cure inflammation.

Inflammation is localized response to foreign substance such as bacteria or in some instance to internally produced substances and has relation with immunity system. The macrophages plays a role in the development of the Iymphohaemopoietic system before and after birth, as well as in the natural and acquired immune responses of adult to immunogens, including infectious agents. NO have been suggested to play an important role in endotoxin-mediated shock and imflammation. In this study, we investigated the effect of Omisodok-eum on the production of NO. The Omisodok-eum inhibited the secretion of NO in LPS-stimulated mouse peritoneal macrophages, without affecting cell viability. The protein level of inducible nitric oxide synlhase(iNOS) in peritoneal macrophages was also decreased by Omisodok-eum. These results suggest that Omisodok-eum suppresses the endotoxin-induced inflammatory responses through inhibiting the production of NO

To elucidate the toxic effect of oxygen free radicals on cultured mouse cerebral neurons damaged by hydrogen peroxide(H₂O₂)-induced neurotoxicity, we examined the neurotoxicity induced by oxygen radicals by NR assay when cultured cerebral neurons were grown in the media containing various concentrations of H202 for 6 hours. In addition, neuroprotective effects of herb extracts such Rhizoma Gastrodiae(RG) on H202-induced neurotoxicity in cultured cerebral neurons were evaluated after cultured cerebral neurons were preincubated with various concentrations of herb extract, RG for 2 hours before 50uM H₂O₂ for 6 hours. H₂O₂ decreased remarkably cell viability in dose-and time-dependent manner in these cultures, and also herb exract, RG decreased LDH activity of cerebral neurons damaged by H₂O₂. From the above results, it is suggested that H₂O₂ was toxic in cultured cerebral neurons from mouse, and RG was effective in blocking the neurotoxicity induced by oxygen radicals in these cultures.

The purpose of this research was to investigate the effect of Yonggak-san (YGS) on the immune reaction and apoptosis of leukemia cells. Administration of YGS(500 mg/kg) enhanced proliferation of splenocytes, thymocytes and mesenteric lymph node cells, and also YGS accelerated subpopulation of splenic Band T, thymic T and mesenteric lymph node-T lymphocytes, especially significantly increased CD4+-TH cells in BALB/c mice. YGS accelerated phagocytic activity and production of nitric oxide in peritoneal macrophages. YGS induced apoptosis of transplanted-L1210 cells in vivo, increased apoptotic cell death of cultured-L1210 and/or Molt4 human leukemia cells, decreased of mitochondrial transmembrane potential of both cells in vitro. These results suggest that YGS have an immune-regulatory effect and anti-cancer property.

We have examined the relaxational response to the water extract of Rheum palmatum L.(ERP) and water extrc alcohol processed Rheum palmatum L.(ARP) in isolated thoracic aorta from sprague dawley (SD) rat. Rat thoracic aort investigated in vessel segments suspended for isometric tension recording by polygraph. Responses to ERP and ARP investigated in vessels precontracted with 5-hydroxytryptamine(5-HT). We found that the thoracic aorta segments respo to ERP and ARP with a dose-dependent vasorelaxation. We found that ; The thoracic aorta segments responded to I and ARP with a dose-dependent vasodilation. The 5-HT induced contraction at 10/sup -4/M were inhibited by 71.7% and a: after addition of the 0.01 g/mL water extract of ERP and ARP. The 5-HT induced contraction at 10/sup -4/M were inhibite 100% after 10/sup -3/M emodin. The concentration of emodin was 0.027% and 0.098% in ERP and ARP. In condusion, ERP ARP induced relaxation in the isolated rat thoracic aorta were composed of dose-dependent relaxation. and it has pi vasodilation.

The purpose of this research was to investigate the effects of Bojunglkgi-tang water extract (BE) on the specific immune response in BALB/c mice. When BE (500mg/kg) was administerd p.o. once a day for 7 days to BALB/c mice, the cell viability of splenocytes was increased and DNA fragmentation of splenocytes was decreased. But, BE did not affect the cell viability and DNA fragmentation of thymocytes. Also, BE increased the population of Thy1/sup +/ cells and TH cells in splenocytes. In addition, BE increased the production of γ -interferon from splenocytes. These results suggest that BE enhances the specific immune response via activation of TH1 cells in splenocytes.

This study was carried out to evaluate Effects of Cheongyoung-tang on CHT-Induced Brain Edema and Neurological Disturbance Symptom in Rats. we make a comparative study of the such parameters as neurological severity score(NSS) and changes in water content, lactate, glucose and hematological substance. 1. ΔNSS increased in CYT1 and CYT2 as compared with the control group, which showed no efficacy. 2. In water content of both hemispheres, CYT1 and CYT2 as compared with the control group have no difference. 3. Water content of the left hemisphere decreased in CYT1 and CYT2 as compared with the control group. 4. The lactate level in serum decreased in CYT1 and CYT2 as compared with the control group. 5. In hematological changes, RBC, PLT and MCHC increased in CYT1 and CYT2 as compared with the control group, but MCV decreased. According to these results, CYT has an effect on CHT-induced brain edema and neurological symptom.

To test the protective effect of herbal medicine on myocardial damage against oxygen free radical-induced myocardiotoxicity, cytotoxicity was examined using MTT, Beating rate and TSARS assay in the presence of water extract of Rhizoma Coptidis. Myocardial toxicity was evaluated in neonatal rat myocardiocytes in cultures. The results of these experiments were obtained as follows: Xanthine oxydase/hypoxanthine resulted in a decrease in viability, beating rate and in a increase in lipid peroxidation in Cultured myocardial cells. Rhizoma Coptidis water extract shows effects of protection from the cardiocyte toxicity induced by xanthine oxydase/hypoxanthine treatment such as increases in beating rate. Rhizoma Coptidis water extract shows effects of protection from the cardiocyte toxicity induced by xanthine oxydase/hypoxanthine treatment such as decreases in lipid peroxidation. These results show that xanthine oxydase/hypoxanthine elicits toxic effects. in cultured myocardial cells derived from neonatal rat, and suggest that water extract of Rhizoma Coptidis is very effective in the prevention of xanthine oxydase/hypoxanthine-induced cardiotoxicity.

Ramulus et Uncus Uncariae (JGD) has sweet in flavour and slightly cold in property, acting on the liver and pericardium channels. This drug was described in a medical classic as having the ability to remove 'heat', check hyperfunction of the liver and relieve dizziness, tremors, and convulsions, and subdue 'endogenous wind'. So this study was estimated to check the anti-neuropathological effect of JGD on the Alzheimer in βAPP overexpression in neuroblastoma cell line and JGD extract was showed significantly anti-alzheimer effects (50 and 100 μg/㎖ of JGD extracts) compared with control group. Ramulus et Uncus Uncariae has anti-alzheimer effects on the βAPP overexpression in neuroblastoma cell line. So we expect that Ramulus et Uncus Uncariae may be used as a drug for neurodegenerative disease, such as stroke, Alzheimer's disease (AD). These results indicate that Ramulus et Uncus Uncariae possess strong inhibitory effect in the nervous system of apoptosis and repair effect against the degeneration of Neuroblastoma cells by βAPP expression.

This study was to investigate the metal accumulation from Ohjeoksan-Decoction to rat brain(Sprague Dawley). 1. In Control group, each metal concentration was within 26.65mg/kg, and in each experimental groups, was within 28.39mg/kg. And, there were no significant metal concentration between control and experimental groups. Exceptionally, Cr and Ni level of control group was lower than experimental groups significantly. (p<0.05) and Cr and Ni level of experimental I group was lower than other experimental groups too.(p<0.05). But, Pb level of control group was higher than experimental groups significantly.(p<0.05) Pb level of experimental I group was higher than other experimental groups(p<0.01). 2. In non-hazardous, hazardous and total metal concentration, there was no increase tendency in brain according to the high dose of OD(Ohjeoksan-Decoction) intake. Reversely, in experimental groups, hazardous metal concentration was decrease by high OD intake.(p<0.05). 3. Correlation among each metal in brain was various in each groups. Briefly under the intake of Ohjeoksan-Decoction, that is very busy herb prescription, this study was defined within safety in brain metal level by P.O. during 10 days. But, there should be a more research about Pb increase by high dose OD intake

The aim of this study was to examine the memory and attention enhancement effect of YMG-1 and YMG-2, which are modified herbal extracts from Yukmijihwang-tang (YMJ). YMJ, composing six herbal medicine, has been used for restoring the normal functions of the body to consolidate the constitution, nourishing and invigorating the kidney functions for hundreds years in Asian countries. A series of studies reported that YMJ and its components enhance memory retention, protects neuronal cell from reactive oxygen attack and boost immune activities. Recently the microarray analysis suggested that YMG-1 protects neurodegeneration through modulating various neuron specific genes. A total of 55 subjects were divided into three groups according to the treatment of YMG-1 (n=20), YMG-2 (n=20) and control (C; n=15) groups. Before treatments, all of subjects were subjected to the assessments on neuropsychological tests of K-WAIS test, Rey-Kim memory test, and psychophysiological test of Event-Related Potential (ERP) during auditory oddball task and repeated word recognition task. They were repeatedly assessed with the same methods after drug treatment for 6 weeks. Although no significant effect of drug was found in Rey-Kim memory test, a significant interaction (P = .010, P < 0.05) between YMG-2 and C groups was identified in the scores digit span and block design, which are the subscales of K-WAIS. The very similar but marginal interaction (P = .064) between YMG-1 and C groups was found too. In ERP analysis, only YMG-1 group showed decreasing tendency of P300 latency during oddball task while the others tended to increase, and it caused significant interaction between session and group (p= .004). This result implies the enhancement of cognitive function in due to consideration of relationship between P300 latency and the speed of information processing. However, no evidence which could demonstrate the significant drug effect was found in neither amplitude or latency. These results come together suggest that YMG-1, 2 may enhance the attention, resulting in enhancement of memory processing. For elucidating detailed mechanism of YMG on learning and memory, the further studies are necessary.

The purpose of this study is to examine the toxic effects caused by xanthine oxidase/hypoxanthine(XO/HX) and the effects of herbal extracts such as JHYJ and GJHYJ on the treatment of the toxic effects. For this purpose, experiments with the cultured hippocampal cells from new born mice were done. The results of these experiments were as follows. 1. XO/HX, a oxygen radical-generating system, decreased the survival rates of the cultured cells on XTT assay and INT assay, the amount of DNA syntheses, and the amount of neurofilaments, and increased the lipid peroxidation. 2. JHYJ and GJHYJ have the efficacy of increasing the survival rates of the cultured cells. 3. JHYJ and GJHYJ have the efficacy of increasing the amount of neurofilaments and of decreasing the lipid peroxidation. 4. JHYJ and GJHYJ have the efficacy of increasing the amount of DNA syntheses. From the above results, it is suggested that Jihwangyeumja and Gamijihwangyeumja have marked efficacy as a treatment for the damages caused by the XO/HX-mediated oxidative stress. And Jihwangyeumja and Gamijihwangyeumja are thought to have certain pharmacological effects. Further dinical study of this pharmacological effects of Jihwangyeumja and Gamijihwangyeumja should be complemented.

This present study was carried out to investigate the body weight-regulatory effects of Chekamhanghyuluiyiin-tang plus Zeae Stigma in high fat diet-induced obese rats. Control group rats were fed with high fat diet and administered normal saline for 8weeks. Experimental groups rats were fed with high fat diet and administered extract of 2 kind of Chekamhanghyuluiyiin-tang plus Zeae Stigma each other for 8 weeks. And observed that, body weight of rats and total cholesterol, triglyceride, free fatty acid, total lipid, phospholipid, high density lipoprotein(HDL)-cholesterol, low density lipoprotein(LDL)-cholesterol in serum of rats, and glucose, insulin of rats. The results were as follows; There were significant decrease of serum free ratty acid level in 4CH/sub 5/ group. There were significant decrease of serum glucose level in 4CH/sub 10/ group. According to above mentioned results, Chekamhanghyuluiyiin-tang plus Zeae Stigma was expected to be applied to the prevention or treatment of obesity and its complications.

The purpose of this research was to investigate the effect of Yonggak-san (YGS) on the anti-allergic reaction in vivo and in vitro. Administration of YGS(500 mg/kg) enhanced hemaggutination(HA)titer against SRBC. On the while, YGS inhibited hyaluronidase activity in vitro and passive cutaneous anaphylaxis reaction, lethal anaphylaxis and mortality induced by compound 48/80 in mice, YGS decreased Arthus reaction, acute hind paw edema induced by histamine. But YGS did not affect delayed type hypersensitivity induced by SRBC. These results suggest that YGS have anti-allergic action

To examine the cytotoxic effect of glucose oxidase(GO) in cultured mouse cerebral neurons, cytotoxicity was measured by MTT assay after cultured nerve cells were incubated for 3 hours in the media containing 1 ～ 60mU/ml concentrations of GO. In addition, the neuroprotective effect of Ramulus et uncus uncariae(REUU) was determined by MTT assay in these cultrures. Cell viability was remarkably decreased in a dose- and time-dependent manner after cultured mouse cerebral neurons were exposed to 30mU/ml GO for 3 hours. In the neuroprotective effect of REUU on GO-induced toxicity, REUU blocked the GO-mediated neurotoxicity in these cultures. From above the results, it suggests that GO is toxic in cultured mouse cerebral neurons and selective herb extract such as REUU is effective in prevetion of the neurotoxicity induced by GO.

The purpose of this research was to investigate the effects of Kamidaebo-tang water extract (KDT) on murine peritoneal macrophages. KDT (50 or 250 mg/kg) was administerd p.o. once a day for 7 days to mice. KDT increased the production of tumor necrosis factor-α and nitric oxide from murine peritoneal macrophages, but decreased the production of interleukin-1β. Also, KDT enhanced the production of lucigenin chemiluminescence from peritoneal macrophages. These results suggest that KDT enhances the non-specific immune response via increase of tumor necrosis factor-α and nitric oxide and phagocytic activity from peritoneal macrophages.

The herbal extract (YMT_02) is a modified herbal extracts from Yukmijihwang-tang (YMJ) to promote memory-enhancing. The YMJ extracts has been widely used as an anti-aging herbal medicine for hundred years in Asian countries. The purpose of this study is to; 1) quantitatively evaluate the memory-enhancing effect of YMT_02 by behavior task, 2) identify candidate genes responsible for enhancing memory by cDNA microarray and 3) assess the anti-oxidant effect of YMT_02 on PC12 cell. Memory retention abilities are addressed by passive avoidance task with Sprague-Dawley (SD) male rat. Before the training session, the rats are subdivided into four groups and administrated with YMT_02, Ginkgo biloba, Soya lecithin and normal saline for 10 days. The retention test was performed. 24 hours after the training session. The retention time of the YMT_02 group was significantly (p<0.05) delayed (～100%), whereas Ginkgo biloba and Soya lecithin treatment delayed 20% and 10% respectively. The hippocampi of YMT_02 and control group were dissected and mANA was further purified. After synthesizing cDNA using oligo-dT primer, the cDNA were applied to Incyte rat GEMTM 2 cDNA microarray. The microarray results show that prealbumin(transthyretin), phosphotidylethanolamine N-methyltransferase, and PEP-19 are expressed abundantly in the YMT_02 treated group. Especially, PEP-19 is a neuron-specific protein, which inhibits apoptotic processes in neuronal cell. On the other hand, transcripts of RAB15, glutamate receptor subunit 2 and CDK108 are abundant in control group. Besides, neuronal genes involved in neuronal death or neurodegeneration such as neuronal-pentraxin and spectrin are abundantly expressed in control group. Additionally, the YMT_02 shows an anti oxidative effect in the PC12 cell. The list of differentially expressed genes may implicate further insight on the action and mechanism behind the memory-enhancing effect of herbal extracts YMT_02, for example, anti-apoptotic, anti-oxidative, and neuroprotective effects.

Ultrasonic nebulizer with the application of new engineering methodology and the design of electronic circuit and 766ppm Cd nebulizing solution were used to generate cadmium aerosol for inhalation toxicology study. The results of particle size analysis for cadmium aerosol were as following. The highest particle counting for source temperature 20℃ was 43.449 x 10³ in inlet temperature 250℃ and particle diameter 0.75㎛. The highest particle counting for source temperature 50℃ was 43.211 x 10³ in inlet temperature 100 ℃ and particle diameter 0.75㎛. The highest particle counting for source temperature 70℃ was 41.917x10³ in inlet temperature 250℃ and particle diameter 0.75㎛. The ranges of geometric mean diameter(GMD) were 0.677-1.009㎛ in source temperature 20℃, 0.716-0.963㎛ in source temperature 50℃, and 0.724-0.957㎛ in source temperature 70℃. The smallest GMD was 0.677㎛ in source temperature 20℃ and inlet temperature 20℃. and the largest GMD was 1.009㎛ in source temperature 20℃ and inlet temperature 20℃. The ranges of geometric standard deviation(GSD) were 1.635-2.101 in source temperature 20℃. 1.676-2.073 in source temperature 50℃, and 1.687-2.051 in source temperature 70℃. The lowest GSD was 1.635 in source temperature 20℃ and inlet temperature 20℃, and the highest GSD was 2.101 in source temperature 20℃ and inlet temperature 200℃. Aerosol generated for cadmium inhalation toxicology study was polydisperse aerosol. The ranges of mass median diameter(MMD) were 1.399-5.270㎛ in source temperature 20℃. 1.593-4.742㎛ in source temperature 50℃, and 1.644-4.504㎛ in source temperature 70℃. The smallest MMD was 1.399㎛ in source temperature 20℃ and inlet temperature 20℃, and the largest MMD was 5.270㎛ in source temperature 20℃ and inlet temperature 200℃. Increasing trends for GMD, GSD, and MMD were observed with same source temperature and increase of inlet temperature. MMD for inhalation toxicology testing in EPA guidance is less than 4㎛. In our results. inlet temperature 20 and 50℃ in source temperature 20℃, and inlet temperature 20 to 150℃ in source temperature 50 and 70℃ were conformed to the EPA guidance. MMD for inhalation toxicology testing in OECD and EU is less than 3㎛. In our results, inlet temperature 20 and 50℃ in source temperature 20, 50, and 70℃ were conformed to the OECD and EU guidance.

Recently, many natural medicines, which have advantage of less side effects and possibility of long-term use, have been studied for their capacity and effects of anti-bacterial, anti-inflammatory and regenerative potential for periodontal tissues. Cortex Eucommiae, Eupoly phaga, Semen Cuscutae, Halloysitum Rubrum have been traditionally used as medicines for treatment of bone disease in Korea. The objective of the present study is to examine the ability of alkaline phosphatase (ALP) activity in human fetal osteoblast cell line (hFOB1) with several natural medicines. hFOB1 added DMEM/F-12 were cultured with dexamethasone as a positive control, and with each natural medicine. ALP activity was measured by spectrophotometer for enzyme activity and naphthol AS-Bl staining was performed for morphometry. All of the natural medicines induced a higher ALP activity compared to negative control, especially, Cortex Eucommiae increased an ALP activity in all experimental groups (p<0.05). In naphthol AS-Bl staining, all of the natural medicines of this study increased the stained area compared to negative control. Especially, Cortex Eucommiae and Eupoly phaga showed statistical significance compared to negative control (p<0.05). These results indicate that Cortex Eucommiae, Eupoly phaga, Semen Cuscutae, Halloysitum Rubrum have an inducing ability of ALP synthesis on osteoblasts.

Silsosangami(SSG) is a formula of oriental medicines as an effective biological response modifier for augmenting host homeostasis of body circulation. Also SSG has been known to have an anti-diabetic activity and anti-platelet aggregation activity. The present study was undertaken to examine the effects of a new SSG on murine macrophage and human neutrophil functions as well as on several enzymes relevant to the inflammatory process. The results of the present study indicate that SSG exerts anti-inflammatory effects related to the inhibition of neutrophil functions and of PGE2 production, which could be due to a decreased expression of and COX-2.

Liuweidihuang-tang has been traditionally used for the treatment of delayed mental and physical development in children, complications of diabetes. and glomerulonephritis. In the present study, the effect of Liuweidihuang-tang on cell proliferation in the dentate gyrus of alcohol-intoxicated Sprague-Dawley rats was investigated via 5-bromo-2' -deoxyuridine (BrdU) immunohistochemistry. Alcohol administration was shown to decrease the number of BrdU-positive cells in the dentate gyrus, while Liuweidihuang-tang treatment increased new cell formation in the dentate gyrus under normal conditions and alcohol intoxication. It was thus demonstrated that aqueous extract of Liuweidihuang-tang exerts a protective effect against alcohol-induced decrease in new cell formation.

LC20 phosphorylation and PKC α play an important role in modulation of contractile activity of smooth muscle. Besides, myosin phosphatase is also related with smooth muscle contraction in signaling pathways. We previously demonstrated that Crataegi Fructus inhibited phenylephrine-induced contraction and which might be implicated in nitrite formation(Son et al., 2002). In this study, we investigated the effects of butanol fraction of Crataegi Fructus(BFFC) on the localization of α-protein kinease C(PKC α) and myosin phosphatase subnits(MPs) in freshly isolated single ferret potal vein cells, and phosphorylation of LC20 during phenylephrine stimulation. In PKC α and MPs localization, BFFC blocked its translocation from the cytosol to the cell membrane by treatment of phenylephrine. BFFC have also dephosphorylated LC20 phosphorylation by phenylephrine stimulation under basal level, but no significant. These results indicate that the relaxation effect of BFFC is associated with inhibition of PKC α activation and MPs dissociation, and thus myosin phosphatase activity may be increased.

Wilson's disease is a rare inborn error of metabolism inherited as a autosomal recessive trait. The disease has varied mode of manifestations. It is characterized by different neurologic disorder and hepatic disease. I experienced a case of Wilson's disease in 40 year old woman who was suffered from liver cirrhosis, severe anorexia, and classical neurologic symptoms such as tremour, dysarthria and ataxia. The symptoms was not relieved by D-penicillamine, Youngyanggaksan and Samchulgunbi-tang but anorexia was improved significantly by same medication.

We observed and treated a 18 year old male who hae been diagnosed with plane wart. Even though he had been treated with western surgery(Laser operating), he still com planed of recurrent plane wart, suffusion, itching. We treated him with ambulatory treatment and herb medication preventing from high fat diet. Treated for 80 days, his symptoms were almost disappeared. Recently he's being treated adult Atopic Dermatitis with satisfaction

Objective: The management for the stroke should ,given as soon as possible to be effect. But Patients with stroke symptoms commonly delay many hours before seeking medical attention. We evaluated the factors which are related to the time of hospital arrival after acute stroke. Method: Data were obtained from 317 patients admitted to our hospital within 72 hours of stroke onset. We assessed demographic variables, stoke subtype. referral routes. history of previous stroke, level of consciousness, distance from the place where stroke occurred to hospital, and the time interval between onset of stroke and arrival at the hospital. Results: Mean patient age was 65.99±9.57 years. The mean time interval between onset of stroke and hospital arrival was 17.26±18.69 hours and 128 (40.38%) patients arrived within 6 hours. The patients whoes stoke subtype was infarction, who arrived our hospital by way of other hospital, who had no suffered from previous stroke and who showed no impairement of consciousness was arrived at the hospital late(p<0.05). Conclusion: The majority of patients arrive at the hospital after prolonged delays for multiple reasons, and patients with milder symptoms, for whom treatment might be more effective, were less likely to arrive in time for therapy. Our study suggest that effective education about stroke to the patients and public would be highly necessary.

Dizziness is a common and often vexing symptom. But the cause of dizziness is very various and generally divided into peripheral and central. Although there are some clinical reports about dizziness. there isn't any report by Constitutional therapy. In this study, the author reports a case of recurrence and persistent vertigo. he was consulted to local E.N.T. and clinically suggested Menier's disease with tinnitus, mild deafness, and vertigo. By using the method of 8 Constitutional Acupuncture therapy and herbal medication(Mihudungsikjang-tang) regarding him as COLONOTONIA. After he regarded and treated as GOLONOTONIA, the symptoms disappeared.