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Cytotoxicity of Hydrogen Peroxide and Effects of Rhizoma Gastrodiae Against Hydrogen Peroxide in Mouse Cerebral Neurons  

Choi Yu Sun (Department of Medicine, Wonkwang University)
Lee Eun Mi (Department of Medicine, Wonkwang University)
Son Young Woo (Department of Medicine, Wonkwang University)
Lee Kang Chang (Professional Graduate School of Oriental Medicine, Wonkwang University)
Shin Yong Il (Department of Medicine, Wonkwang University)
Song Myung Su (Wonkwang health Science College)
Choi Young Ja (Wonkwang health Science College)
Choi Kyu Chul (Wonkwang health Science College)
Kang Hyung Won (College of Oriental Medicine, Wonkwang University)
Lim Chang Yong (College of Oriental Medicine, Wonkwang University)
Rhu Ti Yong (College of Oriental Medicine, Wonkwang University)
Park Sea Hong (College of Oriental Medicine, Wonkwang University)
Park Seung Taeck (Department of Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.5, 2002 , pp. 928-931 More about this Journal
Abstract
To elucidate the toxic effect of oxygen free radicals on cultured mouse cerebral neurons damaged by hydrogen peroxide(H₂O₂)-induced neurotoxicity, we examined the neurotoxicity induced by oxygen radicals by NR assay when cultured cerebral neurons were grown in the media containing various concentrations of H202 for 6 hours. In addition, neuroprotective effects of herb extracts such Rhizoma Gastrodiae(RG) on H202-induced neurotoxicity in cultured cerebral neurons were evaluated after cultured cerebral neurons were preincubated with various concentrations of herb extract, RG for 2 hours before 50uM H₂O₂ for 6 hours. H₂O₂ decreased remarkably cell viability in dose-and time-dependent manner in these cultures, and also herb exract, RG decreased LDH activity of cerebral neurons damaged by H₂O₂. From the above results, it is suggested that H₂O₂ was toxic in cultured cerebral neurons from mouse, and RG was effective in blocking the neurotoxicity induced by oxygen radicals in these cultures.
Keywords
Oxidative stress; Cerebral neuron; Hydrogen peroxide; Rhizoma Gastrodiae;
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