This study was attempted to report the effect of Does for Job's tear on steroid hormone changes in rats. Experimental object was one male rat and four female rats in one group. The experimental diet keeped pace with basal diet and Does of Job's tear. A group was basal diet and basal diet & Does for Job's tea was B group(1/day), C group(2/day). D group(3/day), F group(1 day among 3 day, 3/day), G group (1day among 5 day. 3/day) and E group was made diet & Job's tear(3/day). The reduce of weight was appeared to higher in D group and revealed to higher was F, C, B, G and A group. The changes of steroid hormone were showed to significant difference in A group and indicated to significant difference were G, F, B, C, D and E group.