The levels of psychosocial stresses of dental hygienist were analyzed to reveal the various factors related to them. The self-administered questionnaires were performed, to 276 dental hygienist in Daejeon City. Univariate analysis and multiple regression were performed with survey results, in which a degree of psychosocial stress was dependent variable and others were independent variable. 1. In terms of various levels of psychosocial stress according to the higher level of stress was found in the group of lower age, unmarried, and without spare times for hobby activities, the group with shorter-term job career, lower salary, and stress was found in the group who think that their job is considered as low position or their job is not high position in society or their prospect about job is discouraging, the group without regularly exercise, without regularly eating habits, in the group with higher level of job demand, lower job autonomy, and lower social support from colleagues in work than their respective counterparts. 2. Concerning correlation between psychosocial stress and various factors, while level of stress was negatively correlated with age, job career, salary, socioeconomic status of oneself, socioeconomic status of dental hygienist, future status of dental hygienist, job autonomy, social support from superiors, social support from colleagues, social support, but it was positively correlated with job demand. 3. Multiple regression revealed that the factors which influence on psychosocial stress included hobby activities, sleeping hours, job demand, which has explanatory powers of 29.9%. Therefore, to reduce of stress of dental hygienists, it is required to develop an effective strategy that institutional support for improvement of job environments and research for them be revitalized.