Purpose: This study was designed to investigate enhancing the immunogenicity effects of Platycodon grandiflorum(PG) on adaptive immune system. Methods: To investigate the effect of PG as an adjuvant, we used the ovalbumin (OVA) as an antigen at first. The proliferation of lymphocytes, the antibody titer, the subisotypes of antibodies and the production of cytokines were measured. Results: The proliferation of lymphocytes and the antibody titer were increased after PG treatment. The increased subisotypes of antibodies were IgG2 and IgG3 induced from T1-helper cells. However IgE induced from T2-helper cells was decreased. The production of cytokines derived from T1-helper cells was increased but that from T2-helper cells was decreased. Conclusion: It is supposed that PG has an immunogenicity effect as an adjuvant on adaptive immune system.

Methods: Intravenous administration of Platycodon grandiflorum was performed 2 days before tumor inoculation, then mice were killed 14 days after tumor inoculation, then number of tumor colonies were counted. Methanol extracts of Platycodon grandiflorum was added to colon26-M3.1 carcinoma cells, L5178Y-R lymphoma cells and Hela cells, and then cell growth was counted. To observe the immunomodulating effects of Platycodon grandiflorum, production of IL-6, IL-10, IL-12 and TNF-$\alpha$ were measured with ELISA assay and the cell growth of macrophage were also counted. Furthermore, antimetastatic experiment after depletion NK cells by injection of anti-asialo GM1 serum was also administered. Results: Intravenous administration of Platycodon grandiflorum significantly inhibited metastasis of colon26-M3.1 carcinoma cells. In an in vitro cytotoxicity analysis, Platycodon grandiflorum affected tumor cell growth above specific concentration. As compared with control, the production of IL-6, IL-10, IL-12 and TNF-$\alpha$ were incresed. And depletion NK cell completly abolished the inhibitory effect of metastasis. Conclusion: Platycodon grandiflorum appears to have considerable activity on immunomodulating effects and inhibit the metastasis of tumor. Further evaluation is needed for settling this.

Purpose: These experiments were undertaken to evaluate the effect of administration of Palmulgunjatang gamibang on ovarian functions in female mice. Methods: We administered the Palmulgunjatang gamibang to 6-week-old female CF-1 mice for 4, 8, or 12 days. After administration of Palmulgunjatang gamibang with different concentration, the female mice were injected PMSG and hCG for ovarian hyperstimulation. The mice were divided into 3 different groups for each experiment. To compare the differences, we set a control group treated with plain water at the same volume by the same way. Results: In case of 4-day, 8-day, 12-day administration of Palmulgunjatang gamibang, the mean number of total ovulated oocytes and the number of morphologically normal oocytes were increased compared with control group. We were also examined the embryonic developmental competence in vitro. In case of 4-day administration of Palmulgunjatang gamibang, the rates of blastocyst formation from 2-cell stages were higher than control group. Conclusion: From our results suggested that the medication of Palmulgunjatang gamibang has beneficial effect on reproductive functions of female mice via promotion of cell proliferation.

Purpose: This study was designed to investigate the effects of Cyperi Rhizoma (CR) on changes in weights morphological and histopathological observations in Polycystic Ovary(PCO) rats. Methods: PCO was induced by single intramuscular injection with EV(2mg) in female rats. Normal group(n=8) were injected with sesame oil and orally administrated distilled water for five weeks. PCO control group(n=8) were injected with EV and orally administrated distilled water for five weeks. CR treated group(n=8) were injected with EV and orally administrated CR for five weeks. Then we measured weight of body, ovaries. The histopathology changes of ovaries, and the expression of nerve growth factor(NGF) were also evaluated. Results: Single injection of EV induced suppression of weight gain, formation of cysts, increase of NGF expression. Oral administration of CR prevent suppression of weight gain shown in control group. In addition CR group showed upward tendency of ovarian size. Formation of cystic follicles induced by EV injection is suppressed by CR treatment. In additon, CR treatment lowered expression levels of nerve growth factor(NGF), which were elevated by induction of PCOS. Conclusion: These results suggest that CR can be used for patients with PCOS to prevent formation of cystic follicles and malfunction of ovary. And also suggest that the related mechanisms are involved in suppression of NGF expression.

Purpose: This study was performed to evaluate the single dose oral toxicity of Gwibi-tang extract in ICR mice. Methods: 0(control group), 1250, 2500 and 5000 mg/kg of Gwibi-tang extracts were orally administered to 20 male and 20 female ICR mice. After single oral administration of Gwibi-tang extract to ICR mice, we observed number of the death, clinical signs, changes of body weights for 14 days. After 14 day of Gwibitang extract administration, all mice were sacrificed and major organs were observed. Results: Compared with the control group, we could not find any toxic signs in the mortalities, clinical signs, body weight changes, necropsy findings and hematological values in all treated groups(1250, 2500 and 5000 mg/kg). Conclusions: $LD_{50}$ value of Gwibi-tang extracts may be over 5000 mg/kg and it may have no side toxic effect to ICR mice.

Purpose: To provide basic informations for guidelines of Ginseng usage during lactation. Method: Based on a regulation of scope and preparation of herbal prescriptions by Ministry of health and welfare of Korea, we selected the formulae for postpartum care and disease in 7 Korean Medical Classics. And we searched the number of formulae including Ginseng, dosage and indications of Ginseng in formulae in those books. Results: The range of Ginseng dosage during lactation from medical classics is 1.5-18.75g/day for medical purposes. Indications of Ginseng are lethargy, excessive bleeding, asthma, fever, cold, pain, dizziness, mental disorder, spasm, digestive problem, constipation, diarrhea, urinary incontinence, edema, breast engorgement, lack of breastmilk, breast ulcer, etc. But Ginseng had not been used in the case of stroke, epistaxis, beginning of mastitis, tumor of lower abdomen. Conclusions: Ginseng should be recommended less than 1.5g/d as a nutritional supplement. If breastfeeding woman wants to take Ginseng as a medication or as a tonic, she should consult with a official specialist about proper prescription and dosage instead of risky random medication.

Purpose: To evaluate the effect of Korean medical treatment as supplementary treatment method in ART. Methods: The research was investigated with Korean, Chinese and western medical clinical journals of ART with herbal medicine, acupuncture, and other oriental medical treatment as supplementary treatment. Results: ART with additional herbal treatment achieved notably better result when ovulation rate, quality of oocyte, pregnant rate were compared. In the indepth review and analysis of five journals of ART with acupuncture intervention, firm effective results were not found, however, acupuncture intervention on patients with embryo transplantation had higher achievement rate in artificial insemination. Conclusions: According to the review of the Korean, Chinese, and western medical journals of the effect of Korean medical treatment as supplementary treatment method in ART, acupuncture, herbal medicine, and other oriental medical therapies in ART contribute to the improvement of the outcome of ART. The limit in the research was limited strong enough well-designed RCT studies as evidence for they were very seldom.

Purpose: This survey was done in order to determine Korean medical doctors (K.M.D)' expertise on clinical practice guideline for infertility and the degree of concordance with the guideline. Methods: The questionnaires were distributed via e-mail to all members of Korean Medical Doctors Society and Korean Gynecology Society from August $30^{th}$ to September $1^{st}$ in 2010. Two hundred sixty nine of completed questionnaires were analyzed through two groups, members of the Gynecology Society and nonmembers of the Gynecology Society. Results: According to the analysis of the outcome of the survey done by 99 members of the Korean Gynecology Society and 170 non-members, two-thirds of the participants had prior information of the clinical practice guideline. 63.6 percent of the participants feel strong necessity of the development of Korean medical infertility clinical practice guideline. Seventy percent of the participants are willing to participate in developing or reviewing a clinical guideline. On the question in active utilization and concordance to the guideline once the Korean medical infertility guideline is developed, 44% answered they would while 34.6% said they would utilize the guideline after some modifications. On the prospect of the impact of the development and application of Korean medical clinical practice guideline for infertility, majority of the participants marked positively that the standardization of clinical practice for infertility will lead to the nation's participation with the Nation's Support Work and that the development will somewhat contribute to the standardization of the clinical practice. Conclusions: The survey confirmed high demand of the clinical practice guidelines for infertility and the degree of concordance for infertility amongst Korean Medical doctors.

Purpose: This study was to investigate the correlation among body weight, body composition, delivery method, parity, weight gain during pregnancy and obesity before pregnancy of patients who received postpartum care in one oriental medicine hospital. Mothods: From September 1, 2009 to August 31, 2008, we included 34 postpartum patients who had body composition analysis when admission and discharge among 47 postpartum patients who were hospitalized and received postpartum care in $\bigcirc\bigcirc$ University $\bigcirc\bigcirc$ Hospital. We used SPSS 14.0 for window to test for statistical significance. Results: After postpartum care, body weight and BMI of mothers was significantly decreased and the weight loss was almost body water. Mothers who had cesarean section had relatively severe edema and mothers who had been overweight before pregnancy had high BMI, body fat and abdominal fat. After delivery, body fat percentage was increased highly and body weight retention lasted long in mothers who had gained over 12.5kg during pregnancy. Conclusion: Living habits including diet has a significant effect on weight change of mothers during early puerperium, therefore it is required to teach mothers about breast-feeding, diet, and exercise to help their weight return to normal.

Purpose: The purpose of this study was to examine Bone Marrow Density (BMD) of women suffering from general pain after childbirth. Methods: We studied 18 patients visiting $\bigcirc\bigcirc$hospital from March 2007 to May 2010. The subjects were categorized in two groups, symptom group(n=12) and no symptom group(n=6). We measured BMD using Dual Energy X-ray Absorptiometry (DEXA) and studied the difference of BMD between two groups by Student T-test using SPSS for windows(version 13.0). Results: The general characteristics such as age, days of postpartum, primiparity, cesarean section, breast-feeding of two groups were not different statistically. BMD, T-score and Z-score of symptom group were statistically lower than no symptom group. Conclusion: The results suggest that women suffering from general pain after childbirth shows low BMD, T-score and Z-score. It seems to be due to the kidney deficiency after childbirth.

Purpose: The purpose of this study is to recognize menstrual pattern and dysmenorrhea related factor of first-year women student at college. Methods: From March 22nd to April 2nd, 2010 we researched 382 first-year women students at 00college, aged 19(born after March 1991 to February 1992) by questionnaires to investigate their menstruation and stress. Results: 1. There was statistical correlation between dysmenorrhea and weight and BMI. 2. There was statistical correlation between dysmenorrhea and menstrual amount. 3. There was statistical correlation between dysmenorrhea and menstrual blood clot. 4. There was statistical correlation between dysmenorrhea and stress. Conclusion: This study showed the menstrual pattern and relation between dysmenorrhea and stress. Dysmenorrhea is related to weight, BMI, menstrual amount, menstrual blood clot and especially stress is closely related to dysmenorrhea.

Purpose: The purpose of this study is to know stress and blood stasis pattern for the patients who had hysterectomy related with uterine myoma, adenomyosis, endometriosis. Methods: We got questionnaires from 55 patients among 243 patients who had hysterectomy related with uterine myoma, adenomyosis, endometriosis in the department of obstetrics and gynecology, OO medical center from April 11th 2010 to October 14th 2010, and analyzed them. We conducted correlation analysis between age, pain(VAS), duration of pain and blood stasis score, and between duration of pain, blood stasis score and the sum of Stress Response Index. We compared the sum of Stress Response Index, blood stasis score, VAS according to the result of pathology. For statistics, we used Pearson's correlation coefficient, Mann-Whitney test, oneway analysis of variances(ANOVA) and SPSS version 17.0 for windows. Results: The correlation between duration of pain and blood stasis score showed statistical significance. The correlation between sum of the Stress Response Index and blood stasis score showed statistical significance. There is no statistically correlation between age, VAS, duration of the pain and blood stasis score. There is no statistically significant difference in sum of Stress Response Index, blood stasis score, VAS according to the result of pathology. Conclusion: The result showed that Stress is related with static blood, and the longer patients suffer from pain, the easier patients' blood to be static. Further study will be needed.

Purpose: Women have desired for beauty. And many side effects of plastic surgery have been proven. So safe treatment is expected in recent years. To develop oriental medical treatment on women's cosmetic area, the studies that prove the effect and principle of oriental treatment will be conducted. So, we want to know what kind of research have been done. Methods: We researched for paper which is associated with the cosmetic acupuncture in the Korean journals. The research date is 10th May, 2010. Results: 15 papers were found. Most of papers were written since the year of 2006. And most of papers are written in the Journal of Korean Acupunture & Moxibustion Society. And most of papers deal with subjects about facial acupucture. Conclusion: There is a lack of research on cosmetic area with oriental treatment, so further reseach is needed to comprise large proportion of cosmetic area.

Purpose: The aim of the study was to compare the characteristics of the autonomic innervation of the heart in polycystic ovary syndrome(PCOS) patients with regulary cycling controls. Methods: We studied 21 patients visiting $\bigcirc\bigcirc$hospital from 25th June 2009 to 25th June 2010. The subjects were categorized in two groups, 11 PCOS patients and 10 healthy regularly cycling controls. We studied the difference of Heart rate variability (HRV) between two groups by Independent samples T-test using SPSS for windows(version 17.0). Results: The Standard deviation of all normal R-R intervals (SDNN), The spuare root of the sum of the spuare of difference between adjacent normal R-R intervals (RMS-SD) of PCOS group was non-significantly lower than non-PCOS group. High frequency power (HF) and Normalized high frequency power (HF norm) of PCOS group was significantly higher than non-PCOS group. Normalized low frequency power (LF norm) of PCOS was signficantly lower than non-PCOS group. The results means increased sympathetic and decreased vagal modulation. Total power (TP), Very low frequency power (VLF) of PCOS group was non-significantly lower than non-PCOS group. Low frequency power (LF), LF/HF ratio of PCOS group was non-significantly higher than non-PCOS group. Conclusion: The results suggest that PCOS can be related to decreased activity of parasympathetic nervous system.

Purpose: The purpose of this study is to report the effect of Traditional Korean Medicine therapy for patient in the state of post stillbirth by oligohydramnios Methods: The patient in this case was 33-year-old female who was pregnant by artificial insemination in 2010. Intrauterine growth restriction was discovered at 17 weeks after gestation. And oligohydramnios was found at 20 weeks after gestation. Amnioinfusion was done three times, amniotic fluid infection occurred in the process of amnioinfusion and she had a stillbirth. After stillbirth she came to our clinic with symptoms of abdominal tenderness, distention, mammalgia. The patient was treated by traditional herbal medicine therapy such as Saenghwa-tang(生化湯), Gungguijohyeol-eum(芎歸調血飮), Silso-san(失笑散) and Sasang constitution medicine(荊防地黃湯, 荊防敗毒散, 荊防瀉白散). The progresses of symptoms were evaluated by visual analogue scale. Results: After Traditional Korean Medicine therapy, the patient's symptoms caused by stillbirth was improved. Conclusion: This case study shows that the postpartum treatment in Traditional Korean Medicine therapy and Sasang constitution medicine is effective for the patient in the state of post stillbirth.

Purpose: To review the effectiveness affecting to the shoulder functionalities and upper numbness by Traditional Korean Medical treatment who are given a surgical operation for breast resection by breast tumor Method: To report the patients with dysfunction of shoulder joint and numbness in upper limb after breast surgery who improved by Traditional Korean Medical treatment and to study Traditional Korean Medicine(TKM) management of complications of breast surgery. Results: After about 2weeks treatment, patient's symptoms and signs were improved. TKM management was effective in recovery of shoulder function and upper limb numbness after breast surgery. Conclusion: We need to actively participate in management of complications of breast surgery as adjuvant therapy. And more study is needed for developing Traditional Korean Medical indication of complications of breast surgery.

Purpose: The purpose of this report is to report the clinical application and safety of oriental treatment to pain and symptoms caused by traffic accident during early pregnancy. Methods: The three patients who suffered from several pain and symptoms caused by traffic accident during early pregnancy were treated with herbal medicine, acupuncture, moxibustion, cupping therapy and physical treatment during their admission. And we followed up by phone call after their delivery. Results: Patients' pain and symptoms of this case were improved with oriental treatment during their admission. And serious side effects were not found on the patients and infants after treatment. Conclusion: This report shows that oriental treatment can be effective for those who suffering from several pain and symptoms caused by traffic accident during early pregnancy.

Purpose: To investigate effect of Sweet Bee Venom on postpartum's De Quervain's Disease. Methods: We investigated 3 patients diagnosed as De Quervain's Disease. Sweet Bee Venom Herbal-acupuncture was used for treatment of De Quervain's Disease, once every two days, 5 times. Pain, satisfaction at treatment and function of wrist were measured with VNS, Baumgaertner's nine point scale and dynamometer. Results: Pain reduction, functional improvement, increased grip power were found out in cases treated with Sweet Bee Venom Herbal-acupuncture. In every cases, they represent high subjective satisfaction with treatment. Conclusion: Clinical results show that Sweet Bee Venom Herbal-acupuncture has a good analgesic effect in De Quervain's Disease.