Objectives: The purpose of this study is to investigate the effect of Melandrii Herba (MH), Akebia Quinata Decaisne (AQ), and Tetrapanax Papyriferus (TP) on milk secretion and aquaporin (AQP) expression in lactating mice. Methods: For the experiment, the mice were divided into three groups, which were orally administered MH (2,720 mg/kg), TP (400 mg/kg) and AQ (2,800 mg/kg) extracts respectively for 3 weeks from Day 1 after the birth, compared with the control group (C group), which was administered distilled water. A group consisted of six infantile mice per postpartum mouse. For comparison with the C group, non-pregnant SKH-1 mice were used as the virgin group. Results: 1. When it comes to the immunohistochemical staining for prolactin receptors in the mammary glands, the AQ and MH groups showed a strong immune response to the secretory epithelial cells constituting the mammary alveoli, while the TP group represented a weaker immune response. 2. In the immunohistochemical staining for AQP in the mammary glands, AQP1 showed a strong immune response in the walls of capillaries and venules around the mammary alveoli, and AQP3 in the epithelial cells constituting the mammary alveoli, and AQP5 in some tissues between the mammary alveoli. AQP1 was expressed in the order of TP group>AQ group=C group>MH group, and AQP3 was MH group and AQ group>TP group=C group, and AQP5 was MH group>C group>AQ group and TP group. 3. In the Western blot, AQP1 was expressed in the order of TP group>AQ group>C group>MH group, and AQP3 was MH group>AQ group>C group>TP roup, and AQP5 was MH group>TP Group>C group>AQ group. All of AQP1, 3, 5 expression were significantly higher in the C group than in the Virgin group. Conclusions: The administration of Akebia Quinata Decaisne, Tetrapanax Papyriferus and Melandrii Herba have the effect of improving prolactin levels in postpartum mice and increasing the expression of prolactin receptor and AQPs in the mammary glands, suggesting that lactation might be enhanced by the development of the mammary glands.

Objective: This study was designed to investigate the anti-cancer effects of AAH on ovarian cancer in vitro and by using allograft model in vivo. Methods: In this experiment, the effects of AAH on proliferation rates, cell morphology, cell death type, cell cycle, caspase activities and p38 mitogen-activated protein kinase (MAPK) pathway were investigated in A2780, human ovarian cell line. Results: AAH inhibited proliferation of A2780 cells in a dose dependent manner. In addition, AAH induced apoptosis but did not affect cell cycle of A2780 cells. AAH also effectively inhibited caspase 3 and caspase 9 activities respectively. In allograft tumor model, AAH reduced tumor volume and expanded life span in a dose dependent manner. Conclusion: It can be inferred that AAH can induce apoptosis in ovarian cancer cells and has possibility as an anticancer agent for ovarian cancer.

Objectives: This study was performed to observe the correlations between the results of ABR-2000 and DSOM / 3D-MAC to evaluate the feasibility of ABR-2000 as a oriental medical diagnostic criteria. Methods: We studied 547 women visiting ${\bigcirc}{\bigcirc}$ hospital from December 2012 to June 2015. The subjects were categorized in two groups, 'Hypotonia' and 'Non-Hypotonia' by the result of ABR-2000 and assessed the result of DSOM, 3D-MAC for each group. The differences of pulse wave factors by group also studied. Results: 1. There was no significant difference between two groups about the output frequency of pathogenic factors in DSOM while the result showed the higher correlation in Hypotonia group in terms of the companion tendency of pathogenic factors and syndromes formed by the combination of pathogenic factors. 2. The pulse waves of Hypotonia group were mostly slow, weak, tense and stiff than Non-Hypotonia group. Conclusions: 1. In Hypotonia group, yin deficiency (陰虛) factor was frequently accompanied and consumption (虛損) of various organs based on the yin deficiency (陰虛) was observed. It means chronic and severe condition of exhaustion syndrome (虛勞). 2. The result of 3D-MAC also means pathological feature of yin syndrome (陰 證) and consumption (虛損). Besides, lower scores of Body Surface Area (BSA), body weight, and Body Mass Index (BMI) were associated with body weakness (體瘦), a symptom of exhaustion syndrome (虛勞).

Objectives: This study was conducted to assess the effects of Hwanggimacmundong-tang extract (HM) on obese rats with estrogen deficiency. Methods: The experiments were performed with ovariectomized rats as estrogen-deficient obesity model. Rats were grouped NC (Normal Control Group; sham operation group), OC (Obesity Control Group; estrogen-deficient group), HML (20 mg/kg/day), HMH (100 mg/kg/day). HM was administered orally for six weeks. Body weights and serum lipid level were measured, and real-time PCR was performed to estimate the effect of HM on gene expression in liver. Results: HM decreased the total cholesterol and triglyceride in serum. And HM increased leptin, CPT1 gene expression in liver tissue of obese rats with estrogen deficiency. However HM decreased $PPAR{\gamma}$, $PGC-1{\alpha}$, HMGCR, CYP8B1, LPL, ACAT1, ACAT2, ApoB, ACOX gene expression in liver tissue of obese rats with estrogen deficiency. Conclusions: It is concluded that HM reduced total cholesterol and triglyceride in serum, and regulate gene expression related to lipid metabolism. These results indicate Hwanggimacmundong-tang that might have potential for treatment of obesity and complications during the menopause caused by estrogen-deficiency.

Objectives: The aim of this study is the development of the questionnaire for pattern identification of Liver-qi stagnation infertility. Methods: We conducted a literature review and retrieved the symptoms and diagnosis from Korean and Chinese literatures which mentioned pattern identification of Liver-qi stagnation infertility. Based on the findings, We conducted three times expert Delphi surveys on selection of question items and determination of weight. Results: 12 questionnaire items for subjects and 4 questionnaire items for researchers were selected from 19 references. From expert delphi surveys, we finally determined 14 questionnaire items (10 items for subjects, 4 items for researchers) which are clinically significant and obtained weight of question items. Conclusions: Questionnaire for pattern identification of Liver-qi stagnation infertility was developed through experts' discussion. Further study is required to identify the validity and reliability of this pattern identification instrument for Liver-qi stagnation infertility.

Objectives: The purpose of this study was to investigate the effect of the massage program stimulating the acupuncture points and meridians on the improvement of sleep quality. Methods: 16 subjects suffering from sleep disorders were recruited and the massage program was conducted 3 times a week for a month. VAS (Visual Analog Scale) and KPSQI (Korean Pittsburgh Sleep Quality Index) were collected through questionnaires, and salivary cortisol concentration test and HRV (Heart Rate Variability) were also used to evaluate the effect. The collected data were analyzed by independent statisticians. Results: VAS and KPSQI values decreased significantly after 4 weeks of massage chair application. There was no significant difference in salivary cortisol concentration and HRV test results. In the group with normal HRV test results and salivary cortisol concentration, the change of VAS and KPSQI values was not significant, while the group with abnormal range showed a statistically significant decrease. Conclusions: The massage chair program stimulating meridians according to Korean medicine theory was effective in improving sleep quality. Massage of acupoints and meridians through a massage chair is expected to be useful as an adjunctive therapy in addition to standard Korean medicine therapy.

Objectives: The purpose of this paper is to report the effects of herbal medicine on spontaneous pregnancy with premature ovarian failure. Methods: The 28-year-old patient who was diagnosed premature ovarian failure was treated with herbal medicine (Hyangbujapalmul-tang-gagam), acupuncture and moxibustion. Results: After the treatment, the symptoms of premature ovarian failure such as hot flushes, sweats were improved. The patient recovered menstruation and achieved spontaneous pregnancy. Conclusions: This case shows that Korean medical treatment including herbal medicine has effect on treating infertile female with premature ovarian failure.

Objectives: The aim of this paper is to report that herb medicine effects on four infertility patients with ovarian cysts and the menstrual irregularity as a result of ovarian hyperstimulation syndrome. Methods: Four infertility patients with ovarian cysts had the same symptom of menstrual irregularity for preparing In Vitro Fertilization (IVF). They were treated by twice a day herb medication Changbudodam-tang. Then we observed the effects of treatments by regularity of menstruation, checking with ultrasonography of ovarian cyst size. Results: After herb treatments, the sizes of ovarian cysts decreased and one of them disappeared. Above all, menstrual regularity was recovered for preparing IVF. So they could have a positive progress of IVF. Conclusions: These cases show that herb medicine has its effective treatments for the implantational surroundings on infertility patients with ovarian cyst and menstrual irregularity because of ovarian hyperstimulation syndrome.

Purpose: To report the effect of Korean medical treatments on 1 patient with secondary amenorrhea after ovarian surgery for bilateral endometriomas diagnosed as premature ovarian failure. Methods: The patient who had secondary amenorrhea was treated with Korean medical treatments such as herbal medication, herbal acupuncture and moxibustion therapy, and so forth. Results: After the Korean medical treatments, the patient could have her periods again. And high FSH level was decreased to normal. Conclusions: The case report shows that Korean medical treatment can be an effective option for treating secondary amenorrhea after ovarian surgery for bilateral endometriomas diagnosed as premature ovarian failure.