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Chemical Components and Antioxidative Effects of Eriobotrya japonica Lindl. Leaf (비파잎의 이화학적 성분과 항산화효과)

  • Hwang, Yun-Gyeong;Lee, Jae-Joon;Kim, Ah-Ra;Lee, Myung-Yul
    • Journal of Life Science
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    • v.20 no.11
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    • pp.1625-1633
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    • 2010
  • This study was carried out to investigate the physicochemical compositions and antioxidative effects of Eriobotrya japonoca Lindl (Loquat). The proximate compositions of the loquat leaf on a dry matter basis were 8.78% moisture content, 6.74% crude protein, 7.87% crude fat, 6.99% crude ash, 43.61% dietary fiber and 26.01% carbohydrate. In analysis of free amino acids, 16 kinds total amino acid components, 17 kinds of components were isolated from loquat. The essential amino acids contained in loquat leaf accounted for 50.15% of total amino acids, while the non-essential amino acids accounted for 49.85%. In analysis of total fatty acids, only 5 kinds of acid were detected: lauric acid, myristic acid, pentadecanoic acid, stearic acid and oleic acid. The contents of vitamin A, vitamin E and vitamin C were 0.039 mg%, 0.096 mg% and 0.575 mg%, respectively. The mineral contents of loquat leaf were greater in order of Zn

Improvement of a Tree Cutting Permit System with Respect to Timber Logger's Consciousness (벌채업 관련자의 의식 조사를 통한 현행 벌채제도의 개선)

  • Park, Kyung-Seok;Lee, Seong-Youn;Choi, In-Hwa;Kim, Hyun-Sig;Ahn, Young-Sang;An, Ki-Wan
    • Journal of Korean Society of Forest Science
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    • v.101 no.4
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    • pp.710-721
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    • 2012
  • This study is to find out the search of the desirable tree cutting permit system by investigating and analyzing timber logger's consciousness in Korea. A sample group, including 64 officers in 5 Regional Forest Services and 27 National Forest Stations, 161 local government of 322 officers and team leaders with cutting permits, and 308 tree loggers was surveyed. The survey results showed that the ongoing harvest year plan for national, public and private-type forests, which was applied differently, could be integrated as 3.73 points, which was not significant among the groups. A total of 95.1% of the national forest officers stated that the environmentally friendly harvesting system could be improved and that it is exempted from bad broadleaf trees and renewal of forest type (4.14 points). An environmentally friendly harvesting system including the type of forest and location status (slide, soil, etc) is needed (3.87 points). Additionally, the round timber purchased from tree loggers managed in 2009 was about 10.6% of the domestic timber supply ($3,176,000m^3$) and round timber sales were about 50.1%. A total of 72% of the loggers suggested that a environmentally friendly harvesting system is needed (4.11 points). These results show that a new system for harvesting timber is needed to replace the current environmentally unfriendly harvesting system, and that tree loggers should be registered for management.

Molecular Breeding of Tobacco Plants Resistant to TMV and PVY (분자생물학적 TMV 및 PVY 저항성 연초 육종)

  • E.K. Pank;Kim, Y.H.;Kim, S.S.;Park, S.W.;Lee, C.H.;K.H.Paik
    • Proceedings of the Korean Society of Tobacco Science Conference
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    • 1997.10a
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    • pp.134-152
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    • 1997
  • Plant viruses of tobacco including tobacco mosaic virus (TMV) and potato virus Y (PVY) cause severe economic losses in leaf-tobacco production. Cultural practices do not provide sufficient control against the viruses. Use of valuable resistant cultivars is most recommendable for the control of the viruses. However, conventional breeding programs are not always proper for the development of virus-resistant plants mostly owing to the frequent lack of genetic sources and introduction of their unwanted properties. Therefore, we tried to develop virus-resistant tobacco plants by transforming commercial tobacco cultivars, NC 82 and Burley 21, with coat protein (CP) or replicase (Nlb) genes of TMV and PVY necrosis strain (PVY-VN) with or without untranslated region (UTR) and with or without mutation. Each cDNA was cloned and inserted in plant expression vectors with 1 or 2 CaMV 35S promotors, and introduced into tobacco leaf tissues by Agrobacterium tumefaciens LBA 4404. Plants were regenerated in kanamycin-containing MS media. Regenerated plants were tested for resistance to TMV and PVY In these studies, we could obtain a TMV-resistant transgenic line transformed with TMV CP and 6 genetic lines with PVY-VN cDNAs out of 8 CP and replicase genes. In this presentation, resistance rates, verification of gene introduction in resistant plants, stability of resistance through generations, characteristics of viral multiplication and translocation in resistant plants, and resistance responses relative to inoculum potential and to various PVY strains will be shown. Yield and quality of leaf tobacco of a promising resistant tobacco line will be presented.

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Effects of Whey Protein Hydrolysates on Lipid Profiles and Appetite-Related Hormones in Rats Fed High Fat Diet (고지방식이를 섭취한 흰쥐에서 유청단백질 가수분해물의 섭취가 지질 농도 및 식욕 관련 호르몬에 미치는 영향)

  • Park, Jung-Yoon;Park, Mi-Na;Choi, You-Young;Yun, Sung-Seob;Chun, Ho-Nam;Lee, Yeon-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.4
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    • pp.428-436
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    • 2008
  • This study was carried out to compare the effects of whey protein concentrate, its hydrolysates and macropeptide fractions obtained from papain treatment of whey protein on lipid levels and appetite-related hormones in obesity model rats induced by high fat diet. Four week-old male Sprague-Dawley rats were fed high fat (18% w/w) and low protein (10% w/w) diet for 4 weeks and then divided into four groups (n=8/group). Rats were fed high fat diets containing various nitrogen sources; 10% whey protein concentrate (10WPC), 25% whey protein concentrate (25WPC), 25% whey protein hydrolysates (25WH), and 25% whey macropeptide fractions (25WP, MW$\geq$10,000), respectively for 6 weeks. There were no significant differences in body weight gain and food intake among groups. A significant decrease of total lipid, triglyceride in serum was observed in 25WH and 25WP groups. Total lipid and triglyceride contents of the liver were significantly decreased in 25WPC, 25WH and 25WP groups compared with 10WPC group. However, in the liver, there were no differences in the contents of total lipid and triglyceride among 25WPC, 25WH and 25WP groups. The daily amounts of feces were significantly increased in 25WH and 25WP groups and the excretion of total lipid and triglyceride were significantly increased in 25WH group. Serum glucose and insulin concentration were significantly decreased in 25WH group. The concentration of serum ghrelin was significantly decreased in the 25WPC, 25WH and 25WP groups compared with 10WPC group. However, there was no significant difference in the concentration of serum leptin among groups. These results suggest that whey protein hydrolysates and macropeptide fractions may show beneficial effects on the lipid profile in serum and liver, appetite regulation and insulin resistance in obesity model rats induced by high fat diet.

Mulberry leaf yield and optimal amount of silkworms rearing in different mulberry cultivars for mulberry fruit production (오디 생산용 뽕나무 품종별 뽕잎 생산량 및 적정 누에 사육량)

  • Lim, Ju Rak;Moon, Hyung Cheol;Kwon, Suk Ju;Kim, Dong Wan;Kwak, Dong Ok
    • Journal of Sericultural and Entomological Science
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    • v.53 no.2
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    • pp.82-86
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    • 2015
  • This study was carried out to develop the silkworm rearing technique in unused mulberry leaves after harvesting mulberry fruit. The growth of Gwasang No. 2 and Suhyang was very good compared to control cultivar Chungil in leaves size and new branch growth, but new branch and leaves of Daeshim was similar or small to control cultivar Chungil. The number of leaves of Gwasang No. 2 and Suhyang was lower than Chungil, but weight of leaves per tree was heavyer than Chungil. Mulberry leaf yield was Gwasang No. 2 521 kg/10a, Suhyang 189 kg/10a, Daeshim 73 kg/10a, Chungil 1,095 kg/10a. Content of mineral element of all three mulberry cultivars leaf for mulberry fruit production was higher than Chungil in N, P, K, Ca etc. Feeding quantity of silkworm of Gwasang No. 2 and Suhyang was much more than Chungil. Feeding quantity was highest at Suhyang in 96 kg/box. Growth duration of silkworm larvae was not different in all four mulberry culivars but weight of silkworms (5th instar 3rd day) was heavy at Gwasang No. 2 (2.07 g/head) and Suhyang (2.11 g/head) compared to control cultivar Chungil (1.92 g/head). Mortality of silkworms was 14.6% (Gwasang No. 2), 13.3% (Suhyang), 13.9% (Daesim), 12.6% (Chungil) and than higher at elder instar stage. The production amount of silkworm (5th instar 3rd day) was 35.4 kg/box (Gwasang No. 2), 36.6 kg/box (Suhyang), 35.0 kg/box (Daeshim), 33.6 kg/ box (Chungil). Amount of possible rearing silkworms was estimated 3.4box/10 a (Gwasang No. 2), 1.3box/10a (Suhyang), 0.5box/10a (Daeshim), 8.7box/10a (Chungil).

Large scale splitter-less FFD-SPLITT fractionation: effect of flow rate and channel thickness on fractionation efficiency (대용량 중력장 SPLITT Fractionation: 분획효율에 미치는 채널 두께와 유속의 영향)

  • Yoo, Yeongsuk;Choi, Jaeyeong;Kim, Woon Jung;Eum, Chul Hun;Jung, Euo Chang;Lee, Seungho
    • Analytical Science and Technology
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    • v.27 no.1
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    • pp.34-40
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    • 2014
  • SPLITT fractionation (SF) allows continuous (and thus a preparative scale) separation of micronsized particles into two size fractions ('fraction-a' and 'fraction-b'). SF is usually carried out in a thin rectangular channel with two inlets and two outlets, which is equipped with flow stream splitters at the inlet and the outlet of the channel, respectively. A new large scale splitter-less gravitational SF (GSF) system had been assembled, which was designed to eliminate the flow stream splitters and thus is operated by the full feed depletion (FFD) mode (FFD-GSF). In the FFD mode, there is only one inlet through which the sample is fed. There is no carrier liquid fed into the channel, and thus prevents the sample dilution. The effects of the sample-feeding flow rate, the channel thickness on the fractionation efficiency (FE, number % of particles that have the size predicted by theory) of FFD-GSF was investigated using industrial polyurethane (PU) latex beads. The carrier liquid was water containing 0.1% FL-70 (particle dispersing agent) and 0.02% sodium azide (used as bactericide). The sample loading rate was varied from about 4 to 7 L/hr with the sample concentration fixed at 0.01%. The GSF channel thickness was varied from 900 to $1300{\mu}m$. Particles exiting the GSF channel were collected and monitored by optical microscopy (OM). Sample recovery was monitored by collecting the fractionated particles on a $0.45{\mu}m$ membrane filter. It was found that FE of fraction-a was increased as the channel thickness increases, and FE of fraction-b was increased as the flow rate was increased. In all cases, the sample recovery has higher than 95%. It seems the new splitter-less FFD GSF system could become a useful tool for large scale separations of various types of micron-sized particles.

Effects of Semen Characteristics, Frozen-Thawed Sperm Viability and Serum FSH, LH, Estradiol-17$\beta$ and Testosterone Concentrations between Breeds and among Seasons in Boars (종모돈의 정액성상, 동결-융해 후 정자의 생존성, 혈청 중 FSH, LH, Estradiol-17$\beta$ 및 Testosterone 농도에 미치는 품종과 계절의 영향)

  • Park C. S.;Sung N. D.;Kim C. H.;Jin D. I.;Choi Y. S.;Yi Y. J.
    • Reproductive and Developmental Biology
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    • v.29 no.1
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    • pp.25-30
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    • 2005
  • This study was carried out to investigate the effects of semen characteristics, frozen-thawed sperm viability and serum FSH, LH, estradiol-17β and testosterone concentrations between breeds and among seasons in boars. In all seasons, Yorkshire boars produced higher semen volume compared with Duroc boars, whereas sperm concentration did not differ significantly between Duroc and Yorkshire boars. Semen volume in spring was higher compared with summer, autumn and winter in both Duroc and Yorkshire boars, but sperm concentration did not differ significantly among seasons. Sperm motility and normal acrosome rate of frozen-thawed sperm produced in spring were higher than those in summer, autumn and winter in both Duroc and Yorkshire boars. Sperm motility of frozen-thawed sperm in Yorkshire boars was higher than that in Duroc boars regardless of seasons. However, normal acrosome rate did not differ significantly between Duroc and Yorkshire boars. Serum FSH concentration in Yorkshire boars was lower than that in Duroc boars in all seasons. However, there were no significant differences on serum FSH concentration of Duroc and Yorkshire boars among seasons. Serum LH and estradiol-17β concentrations did not differ significantly between Duroc and Yorkshire boars. Also, there were no significant differences in serum LH and estradiol-17β concentrations of Duroc and Yorkshire boars among seasons. Serum testosterone concentration in Yorkshire boars was higher than that in Duroc boars in all seasons. In both breeds, serum testosterone concentrations were higher in spring than in summer, autumn and winter. In conclusion, when serum FSH concentrations were low, semen volumes were high, and when serum testosterone concentrations were high, sperm motility and normal acrosome rate of frozen-thawed sperm were high.

Comparison of sheep erythrocytes and Korean native goat erythrocytes-rosette forming rate of pig peripheral blood mononuclear cells (돼지 말초혈액 단핵세포의 면양 및 재래산양 적혈구 rosette 형성능 비교)

  • Kim, Young-jin;Song, Hee-jong;Kim, Jong-myeon;Kang, Myeong-dai;Yoon, Chang-yong;Kim, Tae-joong
    • Korean Journal of Veterinary Research
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    • v.32 no.2
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    • pp.175-179
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    • 1992
  • To develope the methods for isolation and enumeration of lymphocyte subpopulations in pigs, we carried out the rosette-assay using sheep erythrocytes(SRBC) and Korean native goat erythrocytes(GRBC) as a target cells. To enumerate T lymphocytes, E-rosette methods were applied with RBC treated with various concentration of polymers such as Aet and Dex, singly or in combination. And to enumerate B lymphocytes, EAand EAC-rosette assay was adopted. The results were as follows; 1. E-RFR with polymer-untreated SRBC and GRBC was $32.9{\pm}7.9%$ and $31.3{\pm}9.4%$, respectively. On the other hand, RFR with 0.1M Aet plus 8% Dex treated SRBC and GRBC was increased about two-fold($67.8{\pm}7.4%$ and $69.8{\pm}8.5%$), respectively. 2. EA-RFR with SRBC and GRBC were $ 39.1{\pm}10.2%$ and $32.6{\pm}6.1%$, respectively. 3. EAC-RFR with SRBC and GRBC were $27.6{\pm}7.0%$ arld $21.0{\pm}3.2%$, respectively. These results showed that both SRBC and GRBC could be recommanded as a binding cells for rosetteassay to isolate of lymphocyte-subpopulations in pigs.

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Studies on the Foliar Application of Urea as Nitrogen Source of Rice Plant Nutrition (요소엽면살포(尿素葉面撒布)에 따른 수도(水稻)의 질소영양(窒素營養)에 관(關)한 연구(硏究))

  • Cho, Seoung-Jin
    • Applied Biological Chemistry
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    • v.9
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    • pp.125-147
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    • 1968
  • This experiment was carried out as a part of the studies on reasonable application of nitrogen in rice plant to determine: (I) Nitrogen absorption. and rooting of rice seedlings as affected by urea foliar application at late seedling stage (II) Effect of leaf prunning and foliar application of urea at late heading stage on the maturation and yield of rice (III) Effect of foliar application of urea and its time during the stage of ear formation on yield of rice plant. Results obtained are summarized as follows. Exp.I: Nitrogen absorption and rooting of rice seedlings as affected be urea foliar application at late seedling stage. 1 : The foliar application of urea plots$(T_{1},T_2)$ snowed mare N-content than non-urea foliar application plot(T0) at lane seedling stage, being significant among treatments and foliar application of urea seemed more effective in increasing the N-content of seedlings. and promoted root settlement and early growth alter the transplanting. 2 : The carbon contents of the plants of $T_1$, and $T_2$ at late seedling stage increased than T0, and the carbon contents. of $T_1$ and $T_2$ plots became higher in amount in proportion to the nitrogen absorption as compared with those of $T_0$. 3 : C/N ratio appeared significant among soil application plots($N_1, \;N_2$) and foliar application of urea plots ($T_1$, $T_2$ and $T_0$). C/N ratio was lower in case of increased amount of nitrogen. The higher contents of nitrogen and carbon and lower C/N ratio resulted in the increment of root numbers and root lengths. Exp.II: Effect of leaf prunning and foliar application of urea at late heading stage on the maturation and yield of rice. 1 : There was a highly significant decrease in the maturing rate by severe leaf prunning. In the mean time, significant increase in maturing rate was observed with urea foliar application and it was found the more frequent application the more effective for higher maturing rate with a moderate significance. A correlationship between the level of prunning and maturing rate was enumerated to 0.961 of correlation coefficient, which indicated an increased maturing rate by the increased number of remaining leaves. 2 : The 1.000 grain weight, grain weight and hulled rice yield increased by leaf prunning in order (plot a$A_1$, $A_3$, $A_2$ and $A_0$ were 89.8%, 89.4%, 87.8% and 87.5% respectively, showing the highest of rate in $A_1$ and $A_3$ in methods of ear fertilization and being highly significant between its treatment. 3 : 1000 grain weights were highly significant between time of application, showing a tendency of increase of weights with the time lagging until days before earings as that of maturing rates. High significance was recognized between methods of ear fertilization, showing the highest in $A_2$ 23.18 gr. 4 : Yields per $3.3m^2$ were not significant between time of ear fertilization, whereas were highly significant between methods of ear fertilization. Those of $A_1$, $A_3$, $A_2$ and $A_0$ were 1.486 kg, 1.491 kg, 1.381 kg and 1.328 kg, respectively, showing the highest in $A_1$ and $A_3$. 5 : Hulling ratios showed significant different between time of ear fertilization, showing the highest in $T_2$, whereas those of methods of ear fertilization were highly significant between its treatment, Those of $A_1$, $A_3$, $A_2$ and $A_0$ were 84.72%, 84.06%, 83.29%, and 82.56% respectively, showing the highest m $A_2$ and $A_3$ among others. 6 : Yields of hulled rice per $3.3m^2$ showed significant different between time of ear fertilization, showing the highest in $T_1$ 1.192 kg. Whereas, those were highly significant between methods of ear fertilization. Those of $A_1$, $A_3$, $A_2$ and $A_0$ were 1.259 kg, 1.254 kg, 1.149 kg and 1.095 kg, respectively, showing the highest in $A_1$ and $A_2$. 7 : Contents of nitrogen on rice plant increased in case of nitrogen application as ear fertilizer and showed that the case of urea foliar application was more effective than that of soil application, showing the increased nitrogen content of rice plant was accompanied by carbon content.

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Studies on Development of Reticulum and Abomasum with Age, and Their Relationship in Korean Native Young Goats (한국 재래유산양(幼山羊)의 제2위와 제4위의 일령 별 발달과 상호관계에 관한 연구)

  • Ha, J.K.;Lee, J.G.;Chang , H.H.;Kim, B.W.
    • Journal of Animal Science and Technology
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    • v.45 no.6
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    • pp.967-974
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    • 2003
  • This experiment was carried out to investigate the relationship of morphological characteristics between reticulum and abomasum of Korean native young goats age from 2 days to 150 days. Number of traits investigated in the reticulum in this experiment were 12[body weight, chest girth, body length, right and left reticulum length(R.L.), upper and lower reticulum length(U.L.), reticulum weight(R.W.), reticulum area(R.A.), upper and lower length of one polygon located at central part of reticulum(U.P.C.R.), right and left length of one polygon located at central part of reticulum(R.P.C.R.), thickness of polygon wall located at central part of reticulum(T.P.C.R.), thickness of polygon wall located at middle part of reticulum(T.P.M.R.), and thickness of polygon wall locared at edge part of reticulum(T.P.E.R.)] and items for abomasum were 12[length of between ostium omaso-abomasicum part and pylosica part in the abomasum(L.B.O.P.), broadest outer part of the abomasum(B.O.A.), weight of abomasum(W.O.A.), area of abomasum(A.O.A.), number of plicae abomasi in the abomasum(N.P.A.A.), thickness of abomasum well at cranial part(ostium omasoabomasicum) in the abomasum(T.A.C.A.), thickness of abomasum well at central part in the abomasum(T.A.P.A.), thickness of abomasum wall at light upper area of pylosica part in the abomasum(T.A.L.A.), length measured from the longest plica abomasi in the abomasum(L.L.P.A.), broadest measured from the longest plica abomasi in the abomasum(B.L.P.A.), area measured from the longest plica abomasi in the abomasum(A.L.P.A.), weight of longest plica abomasi in the abomasum(W.L.P.A.)]. The results were summarized as follows: 1. Number of coefficient of correlation obtained among 12 traits of the abomasum and 12 of the reticulum were 144, and coefficient of correlation of 114 were significant(P〈0.05). 2. Trait of abomasum weight have high correlation with 12 traits of reticulum. 3. Correlation coefficients and regression equation between body weight. VS. abomasum weight(r$_1$), and upper and lower length of one polygon located at central part of reticulum(U.P.C.R.) VS. abomasum weight(r$_2$) were r$_1$=0.8954$^{**}$ and Y=10.703+3.374X, r$_2$=0.8430$^{**}$ and Y=5.689+4.311X, respectively. 4. Correlation coefficients and regression equation between chest girth VS. abomasum weight(r$_1$), and body weight VS. abomasum weight(r$_2$) were r$_1$=0.8708$^{**}$ and Y=-17.219+1.227X, r$_2$=0.8589$^{**}$ and Y=- 17.616+1.290X, respectively.