Korean Journal of Veterinary Research (대한수의학회지)
- Volume 32 Issue 2
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- Pages.175-179
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- 1992
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Comparison of sheep erythrocytes and Korean native goat erythrocytes-rosette forming rate of pig peripheral blood mononuclear cells
돼지 말초혈액 단핵세포의 면양 및 재래산양 적혈구 rosette 형성능 비교
- Kim, Young-jin (College of Veterinary Medicine, Chonbuk National University) ;
-
Song, Hee-jong
(College of Veterinary Medicine, Chonbuk National University) ;
- Kim, Jong-myeon (College of Veterinary Medicine, Chonbuk National University) ;
- Kang, Myeong-dai (College of Veterinary Medicine, Chonbuk National University) ;
- Yoon, Chang-yong (College of Veterinary Medicine, Chonbuk National University) ;
- Kim, Tae-joong (College of Veterinary Medicine, Chonbuk National University)
- 김영진 (전북대학교 수의과대학) ;
-
송희종
(전북대학교 수의과대학) ;
- 김종면 (전북대학교 수의과대학) ;
- 강명대 (전북대학교 수의과대학) ;
- 윤창용 (전북대학교 수의과대학) ;
- 김태중 (전북대학교 수의과대학)
- Received : 1991.04.30
- Published : 1992.04.30
Abstract
To develope the methods for isolation and enumeration of lymphocyte subpopulations in pigs, we carried out the rosette-assay using sheep erythrocytes(SRBC) and Korean native goat erythrocytes(GRBC) as a target cells. To enumerate T lymphocytes, E-rosette methods were applied with RBC treated with various concentration of polymers such as Aet and Dex, singly or in combination. And to enumerate B lymphocytes, EAand EAC-rosette assay was adopted. The results were as follows; 1. E-RFR with polymer-untreated SRBC and GRBC was
돼지(n=22) 순환혈액내 단핵세포(PB-MNCs)중 T 및 B 림프구를 정량하고자 rosette 형성기법을 적용하였다. T 림프구는 여러 농도의 2-aminoethylisothiouronium bromide(Aet)나 dextran(Dex) 용액 또는 Aet와 Dex로 조합 처리된 면양(SRBC) 및 재래산양(GRBC)의 적혈구를 사용한 E-rosette 법으로, B 림프구는 erythrocyeantibody(EA) 및 erythrocyte-antibody-complement(EAC) rosette 법으로 각각 정량하였다. 한편 rosette 형성세포의 냉장정치시간에 따른 판독결과를 비교하여 적정판독시간대를 구명한 바 아래의 결과를 얻었다. 1. PB-MNCs와 SRBC/GRBC와의 자연 rosette형성을(RFR)은
Keywords
- Rosette assay;
- sheep erythrocytes;
- Korean native goat erythrocytes;
- peripheral blood mononuclear cells;
- pig