Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effects of Semen Characteristics, Frozen-Thawed Sperm Viability and Serum FSH, LH, Estradiol-17$\beta$ and Testosterone Concentrations between Breeds and among Seasons in Boars

종모돈의 정액성상, 동결-융해 후 정자의 생존성, 혈청 중 FSH, LH, Estradiol-17$\beta$ 및 Testosterone 농도에 미치는 품종과 계절의 영향

  • Park C. S. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Sung N. D. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Kim C. H. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Jin D. I. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Choi Y. S. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Yi Y. J. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University)
  • 박창식 (충남대학교 농업생명과학대학, 형질전환 복제돼지 연구센터) ;
  • 성낙도 (충남대학교 농업생명과학대학, 형질전환 복제돼지 연구센터) ;
  • 김철호 (충남대학교 농업생명과학대학, 형질전환 복제돼지 연구센터) ;
  • 진동일 (충남대학교 농업생명과학대학, 형질전환 복제돼지 연구센터) ;
  • 최양석 (충남대학교 농업생명과학대학, 형질전환 복제돼지 연구센터) ;
  • 이영주 (충남대학교 농업생명과학대학, 형질전환 복제돼지 연구센터)
  • Published : 2005.03.01
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Abstract

This study was carried out to investigate the effects of semen characteristics, frozen-thawed sperm viability and serum FSH, LH, estradiol-17β and testosterone concentrations between breeds and among seasons in boars. In all seasons, Yorkshire boars produced higher semen volume compared with Duroc boars, whereas sperm concentration did not differ significantly between Duroc and Yorkshire boars. Semen volume in spring was higher compared with summer, autumn and winter in both Duroc and Yorkshire boars, but sperm concentration did not differ significantly among seasons. Sperm motility and normal acrosome rate of frozen-thawed sperm produced in spring were higher than those in summer, autumn and winter in both Duroc and Yorkshire boars. Sperm motility of frozen-thawed sperm in Yorkshire boars was higher than that in Duroc boars regardless of seasons. However, normal acrosome rate did not differ significantly between Duroc and Yorkshire boars. Serum FSH concentration in Yorkshire boars was lower than that in Duroc boars in all seasons. However, there were no significant differences on serum FSH concentration of Duroc and Yorkshire boars among seasons. Serum LH and estradiol-17β concentrations did not differ significantly between Duroc and Yorkshire boars. Also, there were no significant differences in serum LH and estradiol-17β concentrations of Duroc and Yorkshire boars among seasons. Serum testosterone concentration in Yorkshire boars was higher than that in Duroc boars in all seasons. In both breeds, serum testosterone concentrations were higher in spring than in summer, autumn and winter. In conclusion, when serum FSH concentrations were low, semen volumes were high, and when serum testosterone concentrations were high, sperm motility and normal acrosome rate of frozen-thawed sperm were high.

본 연구는 종모돈의 정액성상 동결-융해 후 정자의 생존성 그리고 혈청 중 FSH, LH, estradiol-17β 및 testosterone 농도에 미치는 품종과 계절의 영향을 조사하여 우수한 종모돈의 선발을 위한 기초자료를 얻고자 실시하였다. 요크셔종이 듀록종보다 봄, 여름, 가을, 겨울에서 정액량이 많았으며, 정액농도에서는 차이가 없었다. 계절별 정액량은 듀록 및 요크셔종에서 봄철이 여름, 가을 및 겨울철에 비하여 많았고, 정자농도는 차이가 없었다. 듀록종과 요크셔종에서 각각 봄철에 생산한 정자가 여름, 가을 및 겨울철에 생산한 정자보다 동결-융해 후 정자운동성 및 정상첨체 비율이 높았다. 한편 듀록종과 요크셔종에서 동결-융해 후 정자운동성은 모든 계절에서 요크셔종이 높게 나타났으나, 정상첨체에서는 차이가 없었다. 혈청 중 FSH의 농도를 비교한 결과 요크셔종이 듀록종보다 모든 계절에서 낮은 농도를 나타내었다. 그러나 두 품종 모두에서 각각 계절 간에 차이가 없었다. 혈청 중 LH와 estradiol-17β의 농도를 비교한 결과 요크셔종과 듀록종 간에 차이가 없었다. 또한 두 품종 모두에서 계절 간에 차이가 없었다. 종모돈의 품종별, 계절별 혈청 중 testosterone의 농도를 비교한 결과 요크셔종이 듀록종보다 모든 계절에서 높게 나타났다. 또한 두품종 모두에서 각각 봄철이 여름, 가을 및 겨울철에 비하여 혈청 중 testosterone의 농도가 높은 것으로 나타났다. 이상의 결과를 종합하여 보면, FSH의 농도가 낮을수록 정액생산량이 높은 것으로 나타났으며, 혈청 중 testosterone의 농도가 높을수록 동결-융해 정자의 운동성 및 정상첨체의 비율이 높은 것으로 나타났다.

Keywords

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