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Effects of Semen Characteristics, Frozen-Thawed Sperm Viability and Serum FSH, LH, Estradiol-17$\beta$ and Testosterone Concentrations between Breeds and among Seasons in Boars  

Park C. S. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Sung N. D. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Kim C. H. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Jin D. I. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Choi Y. S. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Yi Y. J. (College of Agriculture and Life Sciences, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Publication Information
Abstract
This study was carried out to investigate the effects of semen characteristics, frozen-thawed sperm viability and serum FSH, LH, estradiol-17β and testosterone concentrations between breeds and among seasons in boars. In all seasons, Yorkshire boars produced higher semen volume compared with Duroc boars, whereas sperm concentration did not differ significantly between Duroc and Yorkshire boars. Semen volume in spring was higher compared with summer, autumn and winter in both Duroc and Yorkshire boars, but sperm concentration did not differ significantly among seasons. Sperm motility and normal acrosome rate of frozen-thawed sperm produced in spring were higher than those in summer, autumn and winter in both Duroc and Yorkshire boars. Sperm motility of frozen-thawed sperm in Yorkshire boars was higher than that in Duroc boars regardless of seasons. However, normal acrosome rate did not differ significantly between Duroc and Yorkshire boars. Serum FSH concentration in Yorkshire boars was lower than that in Duroc boars in all seasons. However, there were no significant differences on serum FSH concentration of Duroc and Yorkshire boars among seasons. Serum LH and estradiol-17β concentrations did not differ significantly between Duroc and Yorkshire boars. Also, there were no significant differences in serum LH and estradiol-17β concentrations of Duroc and Yorkshire boars among seasons. Serum testosterone concentration in Yorkshire boars was higher than that in Duroc boars in all seasons. In both breeds, serum testosterone concentrations were higher in spring than in summer, autumn and winter. In conclusion, when serum FSH concentrations were low, semen volumes were high, and when serum testosterone concentrations were high, sperm motility and normal acrosome rate of frozen-thawed sperm were high.
Keywords
Semen characteristics; Sperm viability; FSH; LH; Estradiol-17β; Testosterone;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Diehl JR, Day BN, Stevermer EJ (1979): Artifical Insemination in Swine. Pork Industry Hand book no. 64
2 Hughes P, Varley K (1980): Reproduction in the Pig: Fertility in the male. pp. 187-195
3 Kesler DJ, Garverick HA, Youngquist RS, Elmore RG, Bierschwal CJ (1977): Effects of days postpartum and endogenous reproductive hormones on GnRH- induced LH release in dairy cows. J Anim Sci 45: 797-803   DOI
4 Zanella E, Lunstra D, Wise T, Kinder J, Ford J (1999): Testicular morphology and function in boars differing in concentrations of plasma follicle-stimulating hormone. Biol Reprod 60:115-118   DOI   ScienceOn
5 Bolt DJ, Rollins R, Gulthrie HD (1981): Development of a porcine FSH radioimmunoassay. J Anim Sci 53 (Suppl. 1):298
6 Polge C, Salamon S, Wilmut I (1970): Fertilizing capacity of frozen boar semen following surgical insemination. Vet Rec 87:424-428   DOI   ScienceOn
7 Schinckel AP, Johnson RK, Kittok RJ (1984): Testicular development and endocrine characteristics of boars selected for either high or low testis size. J Anim Sci 58:675-685   DOI
8 Larsson K (1978): Current research on the deep freezing of boar semen. World Rev Anim Prod XIV:59-64
9 Mariscal DV, Wolfe PL, Bergfeld EG, Cupp AS, Kojima FN, Fike KE, Sanchez T, Wehrman ME, Johnson RK, Kittok RJ, Ford JJ, Kinder JE (1996): Comparison of circulating concentrations of reproductive hormones in boars of lines selected for size of testes or number of ovulations in respective control lines. J Anim Sci 74:1905-1914
10 Allrich, RD, Christenson (1981): Age differences in the response to hCG by porcine testicular tissue in vitro. Biol Rerpod 24 (Suppl. 1):132
11 Ford JJ, Wise TH, Lunstra DD (1997): Negative relationships between blood concentrations of follicle-stimulating hormone and testicular size in mature boars. J Anim Sci 75:790-795
12 Pursel VG, Johnson LA (1975): Freezing of boar spermatozoa: Fertilizing capacity with concentrated semen and a new thawing procedure. J Anim Sci 40:99-102   DOI
13 Zirkin BR, Awoniyi C, Griswold MD, Russell LD, Sharpe, R (1994): Is FSH required for adult spermatogenesis? J Androl 15:273
14 FlorCruz SV, Lapwood KR (1978): A longitudinal study of pubertal development in boars. Int J Androl 1:317   DOI
15 Niswender GD, Reichert LE Jr, Zimmerman DR (1970): Radioimmunoassay of serum levels of LH throughout the estrus cycle in pigs. J Endocrinol 87: 576-580   DOI   ScienceOn
16 Johnson LA (1985): Fertility results using frozen boar spermatozoa, 1970 to 1985. First International Conference on Deep Freezing of Boar Semen, Uppsala, pp. 199-222
17 Pursel VG, Johnson LA (1974): Glutaraldehyde fixation of boar spermatozoa for acrosome evaluation. Theriogenology 1:63-68   DOI   ScienceOn
18 Pursel VG (1979): Advances on preservation of swine spermatozoa. In: H. W. Hawk (ed.) Beltsville Symposia Ill. Animal Reproduction, Allanheld, Osmun and Co., Montclair, NJ. pp. 145-157
19 Pursel VG, Park CS (1987): Duration of thawing on post thaw acrosome morphology and motility of boar spermatozoa frozen on 5 ml maxi-straws. Theriagenology 28:683-690   DOI   ScienceOn
20 Granham EF, Crabo BG, Pace MM (1978): Current status of semen preservation in the ram, boar and stallion. Biennial Symp. Anim Repord J Anim Sci 47 (Suppl. II):80-119   PUBMED
21 Romanowicz K, Stupnicki R, Barcikowski B, Madej A, Walach M (1976): Plasma testosterone and LH in growing boars. Proc VIIth Int Congr Anim Reprod Artificial Insem, Cracow, Poland
22 Johnson LA (1980): Artificial insemination of swine: Fertility with frozen boar semen. Int Pig Vet Conger (Copenhagen) pp. 37
23 Lunstra DD, Ford JJ, Wise TH (1996): Selection for extremes in serum FSH concentrations results in reduced testis size and fertility on Meishan and White Composite boars. In: Tumbleson and Schook (Ed.) Advances in Swine in Biomedical Research. pp. 523-531. Plenum Press, New York
24 Britt JH, Kittok RJ, Harrison DS (1974): Ovulation, estrus and endocrine response after GnRH in early postpartum cows. J Anim Sci 37:915-919
25 Von Rohloff D (1973): Ein Beitrag zur Beurteilung der Taeglichen Spermienproduktion bein Ebem der Deutschen Landrasse. Zuchthygiene 8:72-75   DOI
26 Greenwood FD, Hunter WM, Glover JS (1963): The preparation of $^{131}I$-labeled human growth hormone of high specific radioactivity. Biochem J 89:114   DOI
27 Yi YJ, Kwon YA, Ko HJ, Park CS (2002): Effects of diluent component, freezing rate, thawing time and thawing temperature on acrosome morphology and motility of frozen-thawed boar sperm. Asian-Aust J Anim Sci 15(11):1553-1558   DOI
28 SAS Institute Inc (1996): The SAS system for Windows, Release 6.12, Cary, NC, USA