Recently, eco-friendly materials have emerged around the world due to the need for carbon neutrality. For this reason, the increase in demand for natural fibers and eco-friendly manufacturing technology using them are becoming important. In this study, the physical characteristics of spun yarn were compared using undyed wool fibers and dyed wool fibers in the spinning industry. And the structural forms of spun yarn according to the spinning system, such as traditional ring spinning, sirospun, and hybrid sirospun were compared. In the case of single yarn manufactured in the existing ring spinning machine, the difference in the variation coefficient of the yarn count and the irregularity of the undyed and dyed fibers showed a small level. However, the coefficient of variation of yarn count and the irregularity tended to be significantly lowered in the two folded yarn using single yarn. And the difference between tensile strength and tensile elongation decreased by a large difference, and the tensile strength and tensile elongation of the two folded yarn using single yarn tended to increase. In addition, the variation coefficient of the yarn count and the irregularity according to the spinning system tended to increase in the order of ring spinning, sirospun, and hybrid sirospun spinning. However, tensile strength and tensile elongation tended to decrease in the order of ring spinning, sirospun, and hybrid sirospun spinning. Therefore, in the future, it is considered important to design an optimized product that satisfies the core requirements of the product's appearance, physical, and economic conditions in consideration of the technical characteristics expressed in the spinning system.