• Journal of Applied Biological Chemistry
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• v.62 no.2
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• pp.137-141
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• 2019

At concentrations with little or no cytotoxicity, C. arvensis extract indicates the ability of high DPPH radical scavenging, inhibited tyrosinase activity, and decreased melanin content. The treatment of B16F10 cells with C. arvensis extract suppressed the protein expression of tyrosinase a dose-dependent manner. These results suggest that C. arvensis extract inhibits melanin synthesis by inhibiting tyrosinase expression and tyrosinase activity. In addition, C. arvensis extract showed antimicrobial activities against bacteria and yeast. These results indicate that C. arvensis extract may serve as nutraceutical and cosmeceutical agents.

• Fisheries and Aquatic Sciences
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• v.25 no.6
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• pp.311-319
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• 2022

Hyaluronic acid from Liparis tessellatus eggs (HALTE) was grafted with caffeic acid (CA-g-HALTE), ferulic acid (FA-g-HALTE), gallic acid (GA-g-HALTE), and nisin (Nisin-g-HALTE) and investigated for their anti-inflammatory and antioxidant potential in lipopolysaccharides-stimulated RAW 264.7 mouse macrophages. Nitric oxide (NO) generation and prostaglandin E₂ activity were measured after treatment with the grafted HALTE samples. All grafted HALTE samples exhibited more antioxidant activity against 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radicals than 2,2-diphenyl-1-picrylhydrazyl radicals. Nisin-g-HALTE showed the least antioxidant activity. Additionally, the NO assay results showed that all grafted samples had no cytotoxic effect on RAW 264.7 macrophages and reduced macrophage activity after treatment. The most effective concentrations of CA-g-HALTE and FA-g-HALTE were found to be above 100 ㎍/mL. Increased sample concentration resulted in increased activity except with Nisin-gHALTE at 100 ㎍/mL. CA-g-HALTE, FA-g-HALTE, GA-g-HALTE, and Nisin-g-HALTE were found to have antioxidant and anti-inflammatory potential, which can be further explored for use in food, cosmetic, nutraceutical, and biomedical applications.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.7
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• pp.886-890
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• 2008

Levels of minerals, vitamin, amino acids, fatty acids and chemical compositions were determined in the fleshy parts that are used for food. The moisture, crude protein, crude lipid, crude ash and carbohydrate contents of masou salmon were $73.6{\pm}0.4$, $17.7{\pm}0.3$, $3.3{\pm}0.2$, $1.3{\pm}0.1$ and $4.1{\pm}0.2%$, respectively. Potassium was the highest followed by phosphorus, calcium and sodium. The vitamin C and E levels in masou salmon were 600 and $200\;{\mu}g/100\;g$, respectively. Sixteen amino acids were detected and essential amino acids among them are threonine, valine, methionine, isoleucine, leucine, phenylalanine, lysine, arginine, and histidine. Oleic acid was the most abundant fatty acid in masou salmon and polyunsaturated fatty acids were EPA, DHA, linoleic acid, $\alpha$-linolenic acid, $\gamma$-linolenic acid and arachidonic acid. These results may offer a scientific basis for the commercial use of masou salmon.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.4
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• pp.500-505
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• 2010

The antioxidant activity of methanol extracts from five different parts (flesh, peel, core, seed, calyx) of Jangseong Daebong persimmon (Diospyros kaki cv. Hachiya) were evaluated by determining total phenol content (TPC), DPPH radical scavenging activity (RSA), ABTS RSA, and reducing power (RP). The flesh extract gave the highest yield (92.93%) while the lowest yield was obtained from the seed (5.17%). The seed extract showed the highest total phenolic content ($76.47\pm0.009$ mg GAE/g extract), DPPH RSA ($IC_{50}=52.05\pm1.61\;{\mu}g/mL$), ABTS RSA ($IC_{50}=30.94\pm0.41\;{\mu}g/mL$) and RP ($IC_{50}=87.94\pm0.37\;{\mu}g/mL$). In addition, the calyx extract also showed high antioxidant activity. On the other hand, the core extract gave the lowest TPC and all antioxidant assays. In particular, HT-29 cells showed extensive cell death when treated with $500\;{\mu}g/mL$ of calyx extracts. Thus, these results suggest that methanolic extracts of Jangseong Daebong persimmon seed and calyx may serve as a potential source of natural antioxidant for food and nutraceutical application.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.11 no.5
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• pp.189-204
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• 2016

The study is aimed at figuring out the effect of the consumers benefit, attitudes and interest when customers purchase functional food on purchasing intention and product a foundation of competitive power such as a customized product development for a marketing strategy by examining consumers' behavioral patterns. The range of functional food which is a subject of this study is included in the nutraceutical foods, protective foods in addition to biocontrol food. It surveyed about ages of 20 to 60 domestic residents who have taken functional food in order to conduct the study. Data used in this study was collected 353 answers in September 2016, and hierarchical analysis which is a demographic characteristic as control variables was conducted to verify the hypothesis using statistical program SPSS 21.0 The result of this study demonstrates that firstly, psychological pursued benefit secondly, health concern thirdly, health management attitude affects to functional purchasing intention. It is expecting that the result of this study will be used on the basic standards that are grasping and generalizing the needs of customers. In addition, it is also expecting to contribute to improvement of internal and external functional food industry as a functional food material development because it provides empirical customer behavior analysis result.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.282-288
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• 2023

Due to the COVID-19 pandemic, the immuneenhancing health functional food market that protects our bodies from pathogens such as viruses continues to grow. In this study, we aimed to prove the Cheonggukjang, a high-nutrient food with high protein, fat, and dietary fiber content, as an immuneenhancing nutraceutical. Cheonggukjang water extract (CWE) increased the production of nitric oxide, reactive oxygen species, and cytokines such interleukin (IL)-6, IL-1β, and tumor necrosis factor-α without affecting viability in RAW 264.7 cells. Furthermore, CWE significantly upregulated the expression of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cells. CWE enhanced the phosphorylation of I kappa B kinase α/β and I kappa B (IκB)α, as well as the degradation of IκBα. CWE also induced increased phosphorylation of nuclear factor-kappa B p65 and facilitated the redistribution of p65 from the cytoplasm to the nucleus in RAW 264.7 cells. These findings suggest that CWE has potential as a health functional food material that can enhance the innate immune response.

• Korean Journal of Food Science and Technology
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• v.40 no.6
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• pp.696-701
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• 2008

The objective of this study was to determine whether Codonopsis lanceolata or Platycodon grandiflorum ethyl acetate fraction (CLEA or PGEA) protect cells against sodium nitroprusside (SNP)-induced oxidative stress via the expression of various antioxidant systems. The HepG2 cells exposed for 24 hr to 0.5 mM SNP showed a reduction in the cell viability by an MTT assay. Pretreatment with CLEA and PGEA resulted in an inhibition of SNP-induced cell death. In addition, the effects of CLEA and PGEA on the expression of antioxidant systems via RT-PCR analyses was assessed. The levels of catalase (CAT), glucose-6-phosphate dehydrogenase (G6PD) and metallothionein (MT)-1A mRNA were increased after 24 hr of CLEA exposure. The levels of Mn superoxide dismutase CAT, G6PD, MT-1A, and MT-2A mRNA were increased after PGEA treatment. In conclusion, CLEA and PGEA exert indirect antioxidant effects, perhaps via the induction of a variety of antioxidant systems which, may protect cells against oxidative stress.

• Food Science and Preservation
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• v.20 no.4
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• pp.510-517
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• 2013

This study was conducted to investigate the antioxidant and antimicrobial activities of the hot-water and methanol extracts of raw and black Doraji to increase its utilization. In order to prepare the black Doraji, it was steamed for 15 days at a temperature of $60^{\circ}C$ and then it was dried at a temperature of $30^{\circ}C$ for 3 hr. The methanol extract from the black Doraji (BM) contained the highest levels of total polyphenols among the extracts, and the total polyphenol content of the extract from a black Doraji was higher than that of the extract from a raw Doraji. The total flavonoid contents of the hot-water extract from a black Doraji (BW) was the highest (7.94 mgQE/g) among the samples. The DPPH and ABTS radical scavenging activities increased according to the increase in the concentrations of the Doraji extracts. The BM has the highest radical scavenging activity among the extracts. Each extract showed a slight difference in the antibacterial activity according to the tested strains. The Black Doraji showed a higher antimicrobial activity compared to the raw Doraji. The hot-water extracts demonstrated higher activities than the methanol extracts, and the BW revealed the strongest activity. In this study, the black Doraji showed more effect of the antioxidant and anti-microbial activities than the raw Doraji. These results will provide fundamental data for improving the sitological value and the black Doraji can be used as a valuable resource for the development of nutraceutical foods.

• Microbiology and Biotechnology Letters
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• v.43 no.4
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• pp.367-372
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• 2015

This study was aimed at determining the antioxidant and antimicrobial effects of solvent extracts from Asparagus cochinchinensis. The Asparagus cochinchinensis was extracted with water, methanol, ethanol, n-hexane, dichloromethane, ethyl acetate, and ether. The antimicrobial activity of these extracts was determined by modified well diffusion methods against 4 species of respiratory disease bacteria (Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, and Pseudomonas aeruginosa). In addition, the amount of total polyphenol and flavonoid content, and antioxidant activity was evaluated. Ethyl acetate extract of A. cochinchinensis exhibited higher antimicrobial activity against tested microorganisms than water, methanol, ethanol, n-hexane, dichloromethane, and ether extracts. For antioxidant activity, the ethyl acetate extract of A. cochinchinensis exhibited a notable effect on the scavenging of superoxide against DPPH ($IC_{50}=3.81mg/ml$). Finally, the total polyphenol and flavonoid contents were $14{\pm}0.7mg/g$, and $0.50{\pm}0.13mg/g$, respectively. These results can be regarded as basic research into A. cochinchinensis for the prevention of respiratory diseases. The results indicate that A. cochinchinensis may be utilized as a nutraceutical for respiratory diseases when the physiologically active substances of A. cochinchinensis are increased by further study.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.1
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• pp.131-135
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• 2002

This study was carried out to investigate the inhibitory effect of extracts from Paeoniae radix on postprandial hyperglycemia. Organic solvent (hexane, ethyl acetate, butanol, water) extracts from Paeoniae radix were fractionated by high performance liquid chromatography. These fractions were used to screen $\alpha$-glucosidase (EC 3.2.1.20) inhibitors by microplate colorimetric assay. The fractions 11, 12, 18, 19 of ethyl acetate extract from Paeoniae radix showed inhibitory activity by 85%, 84%, 77%, 77% at concentration of 20 $\mu\textrm{g}$/mL, respectively. The selected fractions (no. 10~no. 19) significantly reduced by 22% the blood glucose elevation in comparison with positive control in mice loaded with maltose. The fractions of Paeoniae radix were determined in vitro inhibitory activity on $\alpha$-glucosidase and in vivo inhibition effect on blood glucose elevation in mice. Therefore, these results suggest that the extract of Paeoniae radix can be used as a new nutraceutial for inhibition on postprandial hyperglycemia as well as resource pool for lead compounds as a $\alpha$-glucosidase inhibitor.