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http://dx.doi.org/10.4014/mbl.1511.11009

Antimicrobial Activity against Respiratory Bacteria by Asparagus Cochinchinensis Extracts and its Antioxidant Capacity  

Jung, Min-Gi (Department of Food Science & Technology, Pusan National University)
Kim, Su-In (Department of Food Science & Technology, Pusan National University)
Jeong, Hae-Jin (Department of Food Science & Technology, Pusan National University)
Lee, Chung-Yeol (Kanglim Organic Co., Ltd.)
Son, Hong-Joo (Department of Food Science & Technology, Pusan National University)
Hwang, Dae-Youn (Department of Food Science & Technology, Pusan National University)
Lee, Hee-sup (Department of Food Science & Technology, Pusan National University)
Kim, Dong-Seob (Department of Food Science & Technology, Pusan National University)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.4, 2015 , pp. 367-372 More about this Journal
Abstract
This study was aimed at determining the antioxidant and antimicrobial effects of solvent extracts from Asparagus cochinchinensis. The Asparagus cochinchinensis was extracted with water, methanol, ethanol, n-hexane, dichloromethane, ethyl acetate, and ether. The antimicrobial activity of these extracts was determined by modified well diffusion methods against 4 species of respiratory disease bacteria (Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, and Pseudomonas aeruginosa). In addition, the amount of total polyphenol and flavonoid content, and antioxidant activity was evaluated. Ethyl acetate extract of A. cochinchinensis exhibited higher antimicrobial activity against tested microorganisms than water, methanol, ethanol, n-hexane, dichloromethane, and ether extracts. For antioxidant activity, the ethyl acetate extract of A. cochinchinensis exhibited a notable effect on the scavenging of superoxide against DPPH ($IC_{50}=3.81mg/ml$). Finally, the total polyphenol and flavonoid contents were $14{\pm}0.7mg/g$, and $0.50{\pm}0.13mg/g$, respectively. These results can be regarded as basic research into A. cochinchinensis for the prevention of respiratory diseases. The results indicate that A. cochinchinensis may be utilized as a nutraceutical for respiratory diseases when the physiologically active substances of A. cochinchinensis are increased by further study.
Keywords
Antimicrobial acitivity; respiratory disease; antioxidant activity; Asparagus cochinchinensis; Pseudomonas aeruginosa; well diffusion methods;
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1 Bae KM, Park SH, Jung KH, Kim MJ, Hong SH, Song YO, et al. 2010. Effects of roasting conditions on physicochemical properties and sensory properties of Liriopis tuber. Prev. Nutr. Food Sci. 39: 1503-1508.
2 Choe S-B, Kang S-T 2014. Investigation of antimicrobial activity and stability of Orixa japonica Thunb. leaf extract. Korean J. Food Sci. Technol. 46: 39-43.   DOI
3 Choi O-J. 1991. Component and the use of herbs. Ilwolbooks p.672.
4 Choi SY, Kim SY, Hur JM, Choi HG, Sung NJ. 2006. Antioxidant activity of solvent extracts from Sargassum thunbergii. Prev. Nutr. Food Sci. 35: 139-144.
5 Chung JK, Lee JC, Ha DR. 2014. Antimicrobial activities of sword bean (Canavalia gladiata) extracts against food poisoning bacteria. J. Fd Hyg. Safety 29: 376-382.   DOI
6 Cooperation teaching materials compilation committee of oriental medicine college in Korea. 2005. Herbal medicine. Younglimsa Press. Seoul, Korea. pp.647-648.
7 Dockery DW, Pope CA. 1994. Acute respiratory effects of particulate air pollution. Annu. Rev. Pub. Health. 15: 107-132.   DOI
8 Doh ES. 2010. Antibacterial activity of medicinal plant extracts to S. aureus KCCM12256 and V. parahaemolyticus KCCM11965. J. East Asian Soc. Dietary Life. 20: 881-887.
9 Freeman BA, Crapo JD. 1982. Biology of disease: free radicals and tissue injury. Lab. Invest. 47: 412.
10 Freeman BA, Topolosky MK, Crapo JD. 1982. Hyperoxia increases oxygen radical production in rat lung homogenates. Arch. Biochem. Biophys. 216: 477-484.   DOI
11 Hwang D-Y. 1978. Bangyakapyeon. Namsandang, pp.178, 205.
12 Valderrey AD, Pozuelo MJ, Jimenez PA, Macia MD, Oliver A, Rotger R. 2010. Chronic colonization by Pseudomonas aeruginosa of patients with obstructive lung diseases: cystic fibrosis, bronchiectasis, and chronic obstructive pulmonary disease. Diagn. Microbiol. Infect. Dis. 68: 20-27.   DOI
13 WHO (World Health Organization). Air Pollution including WHO's 1999 Guidelines for Air Pollution Control. Geneva, Switzerland.
14 Woo J, Lee JS, Kwon KH, Kim KH, Choi CH, Park C, et al. 1995. Etiologies of bacterial pneumonia with implications for therapy. Tuberc Respir Dis. (Seoul). 42: 67-75.   DOI
15 Yoo MY, Jung YJ, Yang JY. 2005. Antimicrobial activity of herb extracts. Prev. Nutr. Food Sci. 34: 1130-1135.
16 Yuk JE, Woo JS, Yun CY, Lee JS, Kim JH, Song GY, et al. 2007. Effects of lactose-${\beta}$-sitosterol and ${\beta}$-sitosterol on ovalbumin-induced lung inflammation in actively sensitized mice. Int. Immunopharmacol. 12: 1517-1527.
17 Lee SK, Park JH, Kim YT. 2009. A study on the antioxidation and antimicrobial effect. Prev. Nutr. Food Sci. 22: 279-285.
18 Igusa R, Narumi S, Murakami K, Kitawaki Y, Tamii T, Kato M, et al. 2012. Escherichia coli pneumonia in combination with fungal sinusitis and meningitis in a tsunami survivor after the Great East Japan Earthquake. Tohoku J. Exp. Med. 227: 179-184.   DOI
19 Joo SK, Koo SW, Kim M, Cho YH, Cha ST, Hong GY, et al. 2009. Bacterial etiology in hospitalized patients with acute exacerbations of chronic obstructive pulmonary disease. Korean J. Med. 77: 309-314.
20 Katsouyanni K, Touloumi G, Spix C, Schwartz J, Balducci F, Medina S, et al. 1997. Short term effects of ambient sulphur dioxide and particulate matter on mortality in 12 European cities: results from time series data from the APHEA project. Bmj. 314: 1658.   DOI
21 Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J. Food Sci. Technol. 37: 233-240.
22 Lee SI. 1997. The level of antioxidant enzymes in red blood cells of patients with chronic obstructive pulmonary disease. Tuberc Respir Dis. 44: 104-113.   DOI
23 Mahajan SG, Mehta AA. 2011. Suppression of ovalbumin-induced Th2-driven airway inflammation by ${\beta}$-sitosterol in a guinea pig model of asthma. Eur. J. Pharmacol. 650: 458-464.   DOI
24 Nathan P, Law EJ, Murphy DF, MacMillan BC. 1978. A laboratory method for selection of topical antimicrobial agents to treat infected burn wounds. Burns 4: 177-187.   DOI
25 Sanchez-Moreno C. 2002. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci. Technol. Int. 8: 121-137.   DOI
26 Amerinem MA, Ough CS. 1958. Method for Analysis of Musts and Win. Wiley & Sons. 176-180.
27 Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol. 71: 109-114.   DOI
28 Pandak N, Pajic-Penavic I, Sekelj A, Tomic-Paradzik M, Cabraja I, Miklausic B. 2011. Bacterial colonization or infection in chronic sinusitis. Wien. Klin. Wochenschr. 123: 710-713.   DOI
29 Ryu YH, Kim DG, Yeon KI, Huh CS, Ryu JA, Jo WS, et al. 2015. Screening for inhibition activity of plant extracts on microorganism contaminating in cosmetics. Korean J. Med. Crop. Sci. 23: 57-76.   DOI
30 Sachse F, Von Eiff C, Becker K, Steinhoff M, Rudack C. 2008. Proinflammatory impact of Staphylococcus epidermidis on the nasal epithelium quantified by IL-8 and GRO-${\alpha}$ responses in primary human nasal epithelial cells. Int. Arch. Allergy Immunol. 145: 24-32.   DOI
31 Schwartz J. 1994. What are people dying of on high air pollution days?. Environ. Res. 64: 26-35.   DOI
32 Seo SJ, Kim NW. 2010. Physiological activities of leaf and root extracts from Liriope platyphylla. Korean J. Food Preserv. 17: 123-130.
33 Shin DC. 2007. Health effects of ambient particulate matter. J. Korean Med. Assoc. 50: 175-182.   DOI
34 Statistics Korea. 2014. statistics of death cause. http://kostat.go.kr. Accessed Sep. 23, 2015.