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http://dx.doi.org/10.3746/jkfn.2002.31.1.131

Inhibitory Effect of Extracts from Paeoniae radix on Postprandial Hyperglycemia  

지승택 (제네티카(주) 부설연구소)
이성진 (제네티카(주) 부설연구소)
이강은 (제네티카(주) 부설연구소)
손용태 (제네티카(주) 부설연구소)
정요경 (제네티카(주) 부설연구소)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.31, no.1, 2002 , pp. 131-135 More about this Journal
Abstract
This study was carried out to investigate the inhibitory effect of extracts from Paeoniae radix on postprandial hyperglycemia. Organic solvent (hexane, ethyl acetate, butanol, water) extracts from Paeoniae radix were fractionated by high performance liquid chromatography. These fractions were used to screen $\alpha$-glucosidase (EC 3.2.1.20) inhibitors by microplate colorimetric assay. The fractions 11, 12, 18, 19 of ethyl acetate extract from Paeoniae radix showed inhibitory activity by 85%, 84%, 77%, 77% at concentration of 20 $\mu\textrm{g}$/mL, respectively. The selected fractions (no. 10~no. 19) significantly reduced by 22% the blood glucose elevation in comparison with positive control in mice loaded with maltose. The fractions of Paeoniae radix were determined in vitro inhibitory activity on $\alpha$-glucosidase and in vivo inhibition effect on blood glucose elevation in mice. Therefore, these results suggest that the extract of Paeoniae radix can be used as a new nutraceutial for inhibition on postprandial hyperglycemia as well as resource pool for lead compounds as a $\alpha$-glucosidase inhibitor.
Keywords
Paeoniae radix; postprandial hyperglycemia; ${\alpha}$-glucosidase inhibitor; ${\alpha}$-glucosidase;
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