Journal of Applied Biological Chemistry

  • Volume 66
  • /
  • Pages.282-288
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  • 2023
  • /
  • 1976-0442(pISSN)
  • /
  • 2234-7941(eISSN)
The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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The immune enhancement effect of Cheonggukjang Water Extract (CWE) via activation of NF-κB pathways in murine macrophage RAW 264.7 cells

RAW 264.7 대식세포에서 청국장 열수 추출물(Cheonggukjang Water Extract, CWE)의 면역 증강 효과

  • Sehyeon Jang (School of Food Science and Biotechnology, Kyungpook National University) ;
  • San Kim (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Se Jeong Kim (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Sung Ran Yoon (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research & Extension services) ;
  • Bo Ram So (COSMAX NBT, INC.) ;
  • Jung A Ryu (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research & Extension services) ;
  • Jeong Min Park (Division of Agricultural Environment Research, Gyeongsangbuk-do Agricultural Research & Extension services) ;
  • Sung Keun Jung (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2023.06.10
  • Accepted : 2023.06.30
  • Published : 2023.12.31
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Abstract

Due to the COVID-19 pandemic, the immuneenhancing health functional food market that protects our bodies from pathogens such as viruses continues to grow. In this study, we aimed to prove the Cheonggukjang, a high-nutrient food with high protein, fat, and dietary fiber content, as an immuneenhancing nutraceutical. Cheonggukjang water extract (CWE) increased the production of nitric oxide, reactive oxygen species, and cytokines such interleukin (IL)-6, IL-1β, and tumor necrosis factor-α without affecting viability in RAW 264.7 cells. Furthermore, CWE significantly upregulated the expression of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cells. CWE enhanced the phosphorylation of I kappa B kinase α/β and I kappa B (IκB)α, as well as the degradation of IκBα. CWE also induced increased phosphorylation of nuclear factor-kappa B p65 and facilitated the redistribution of p65 from the cytoplasm to the nucleus in RAW 264.7 cells. These findings suggest that CWE has potential as a health functional food material that can enhance the innate immune response.

코로나19 바이러스의 대유행으로 바이러스와 같은 외부 병원균으로부터 우리의 몸을 보호하는 면역 기능 개선 건강기능식품의 시장은 점차 증가하고 있다. 우리는 본 연구에서 높은 조단백, 조지방, 식이섬유 함량을 나타내는 고영양식품인 청국장이 면역 강화 기능을 나타냄을 밝혀내고자 하였다. 청국장 열수 추출물은 RAW 264.7 세포에서 세포독성을 나타내지 않으며, 대식세포의 nitric oxide, reactive oxygen species 및 interleukin (IL)-6, IL-1β, tumor necrosis factor-α 사이토카인의 생산량을 증가시켰다. 또한, 청국장 열수 추출물은 RAW 264.7 세포에서 inducible nitric oxide synthase 및 cyclooxygenase-2의 발현을 유의적으로 증가시켰다. 청국장 열수 추출물은 RAW 264.7 세포에서 I kappa B kinase α/β와 I kappa B (IκB)α의 인산화 및 IκBα의 degradation을 증가시켰으며, Nuclear factor-kappa B p65의 인산화를 증가시켜 p65의 세포질에서 핵으로의 이동을 촉진하였다. 이러한 연구 결과는 청국장 추출물이 선천성 면역 반응을 강화하는데 유망한 건강기능식품 소재로 활용될 수 있음을 시사한다.

Keywords

Acknowledgement

본 연구는 2022년 경상북도기술원 1팀 1교수 책임제 공동연구 '경북 식재료 활용K-면역 농식품 기술개발' (과제번호: LP0048882022) 연구비 지원과 한국연구재단의 중견연구 (NRF-2022R1A2C1010923) 지원을 받아 수행되었습니다.

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