Browse > Article

Cytoprotective Effect by Antioxidant Activity of Codonopsis lanceolata and Platycodon grandiflorum Ethyl Acetate Fraction in Human HepG2 Cells  

Kim, Soo-Hyun (Department of Food Science and Biotechnology, School of Biotechnology, Kangwon National University)
Choi, Hyun-Jin (Department of Food Science and Biotechnology, School of Biotechnology, Kangwon National University)
Oh, Hyun-Taek (Department of Food Science and Biotechnology, School of Biotechnology, Kangwon National University)
Chung, Mi-Ja (The Nutraceutical Bio Brain Korea 21 Project Group, Kangwon National University)
Cui, Cheng-Bi (Department of Food Science and Engineering, Agricultural College of Yanbian University)
Ham, Seung-Shi (Department of Food Science and Biotechnology, School of Biotechnology, Kangwon National University)
Publication Information
Korean Journal of Food Science and Technology / v.40, no.6, 2008 , pp. 696-701 More about this Journal
Abstract
The objective of this study was to determine whether Codonopsis lanceolata or Platycodon grandiflorum ethyl acetate fraction (CLEA or PGEA) protect cells against sodium nitroprusside (SNP)-induced oxidative stress via the expression of various antioxidant systems. The HepG2 cells exposed for 24 hr to 0.5 mM SNP showed a reduction in the cell viability by an MTT assay. Pretreatment with CLEA and PGEA resulted in an inhibition of SNP-induced cell death. In addition, the effects of CLEA and PGEA on the expression of antioxidant systems via RT-PCR analyses was assessed. The levels of catalase (CAT), glucose-6-phosphate dehydrogenase (G6PD) and metallothionein (MT)-1A mRNA were increased after 24 hr of CLEA exposure. The levels of Mn superoxide dismutase CAT, G6PD, MT-1A, and MT-2A mRNA were increased after PGEA treatment. In conclusion, CLEA and PGEA exert indirect antioxidant effects, perhaps via the induction of a variety of antioxidant systems which, may protect cells against oxidative stress.
Keywords
Codonopsis lanceolata; Platycodon grandiflorum; antioxidant; oxidative stress; antioxidant systems;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 Bartsch H, Nair J. Chronic inflammation and oxidative stress in the genesis and perpetuation of cancer: Role of lipid peroxidation, DNA damage, and repair. Langenbeck Arch. Surg. 391: 499-510 (2006)   DOI
2 Biesalski HK. Free radical theory of aging. Curr. Opin. Clin. Nutr. 5: 5-10 (2002)   DOI   ScienceOn
3 Fukuzawa K, Takaishi Y. Antioxidants. J. Act. Oxyg. Free Rad. 1: 55-70 (1990)
4 Oh HS. Studies on biological activities of the plant resources of Gangwon-do: Focused on the Acer ginnala Max. PhD thesis, Gangwon National University, Chunchon, Korea (2005)
5 Han EG, Cho SY. Effects of Codonopsis lanceolata water extract on the activities of antioxidative enzymes in carbon tetrachloride treated rats. Korean J. Soc. Food Sci. Nutr. 26: 1181-1186 (1997)
6 Lee JY, Hwang WI, Lim ST. Effect of Platycodon grandiflorum DC extract on the growth of cancer cell lines. Korean J. Food Sci. Technol. 30: 13-21 (1998)
7 Rubiolo JA, Mithieux G, Vega FV. Resveratrol protects primary rat hepatocytes against oxidative stress damage: Activation of the Nrf2 transcription factor and augmented activities of antioxidant enzymes. Eur. J. Pharmacol. 591: 66-72 (2008)   DOI   ScienceOn
8 Schmidt AJ, Heiser P, Hemmeter UM, Krieg JC, Vedder H. Effect of antidepressants on mRNA levels of antioxidant enzymes in human monocytic U-937 cells. Prog. Neuro-Psychoph. 32: 1567-1573 (2008)   DOI   ScienceOn
9 Lee KJ, Choi CY, Chung YC, Kim YS, Ryu SY, Roh SH, Jeong HG. Protective effect of saponins derived from roots of Platycodon grandiflorum on tert-butylhydroperoxide-induced oxidative hepatotoxicity. Toxicol. Lett. 147: 271-282 (2004)   DOI   ScienceOn
10 Chung MJ, Walker PA, Hogstrand C. Dietary phenolic antioxidants, caffeic acid and Trolox, protect rainbow trout gill cells from nitric oxide-induced apoptosis. Aquat. Toxicol. 80: 321-328 (2006)   DOI   ScienceOn
11 Oh HM, Kim MK. Effect of dried leaf powders, water, and ethanol extracts of persimmon and green tea leaves on lipid metabolism and antioxidative capacity in 12-month-old rats. Korean J. Nutr. 34: 285-298 (2001)
12 Van Houten B, Woshner V, Santos JH. Role of mitochondrial DNA in toxic responses to oxidative stress. DNA Repair 5: 145-152 (2006)   DOI   ScienceOn
13 Maeng YS, Park HK. Antioxidant activity of ethanol extract from Ddodok (Codonopsis lanceolata). Korean J. Food Sci. Technol. 23: 311-316 (1991)   과학기술학회마을
14 Lee JY, Hwang WI, Lim ST. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J. Ethnopharmacol. 93: 409-415 (2004)   DOI   ScienceOn
15 Hatano T. Constituents of natural medicines with scavenging effects on active oxygen species-tannins and related polyphenols. Nat. Med. 49: 357-363 (1995)
16 Comelli MC, Mengs U, Schneider C, Prosdocimi M. Toward the definition of the mechanism of action of silymarin: Activities related to cellular protection from toxic damage induced by chemotherapy. Integr. Cancer Ther. 6: 120-129 (2007)   DOI
17 Chung MJ, Hogstrand C, Lee SJ. Cytotoxicity of nitric oxide is alleviated by zinc-mediated expression of antioxidant genes. Exp. Biol. Med. 231: 1555-1563 (2006)   DOI
18 Yu BP. Cellular defenses against damage from reactive oxygen species. Physiol. Rev. 74: 139-162 (2004)
19 Chung MJ, Sung NJ, Park CS, Kweon DK, Alberto M, Moon TW, Lee SJ, Park KH. Antioxidative and hypocholesterolemic activities of water-soluble puerarin glycosides in HepG2 cells and in C57 BL/6J mice. Eur. J. Pharmacol. 578: 159-170 (2008)   DOI   ScienceOn
20 Sung IS, Park EM, Lee MK, Han EK, Jang JY, Cho SY. Effects of acorn extracts on the antioxidative enzyme system. J. Korean Soc. Food Sci. Nutr. 26: 494-500 (1997)
21 Kim KS, Osamu E, Shinji I, Hiroshige I. Effects of Platycodon grandiflorum feeding on serum and liver lipid concentrations in rats with diet-induced hyperlipidemia. J. Nutr. Sci. Vitaminol 41: 485-491 (1995)   DOI   ScienceOn
22 Choi MS, Choi PS. Plant regeneration and saponin contents in Codonopsis lanceolata L. Korean J. Med. Crop Sci. 7: 275-281 (1999)
23 Chung MJ, Walker PA, Brown RW, Hogstrand C. ZINC-mediated gene expression offers protection against $H_2O_2$-induced cytotoxicity. Toxicol. Appl. Pharm. 205: 225-236 (2005)   DOI   ScienceOn
24 Yu BP. Aging and oxidative stress: Modulation by dietary restriction. Free Radical Bio. Med. 21: 651-668 (1996)   DOI   ScienceOn
25 Kim SK, Lee HJ, Kim MK. Effect of water and ethanol extracts of persimmon leaf and green tea different conditions on lipid metabolism and antioxidative capacity in 12-month-old rats. Korean J. Nutr. 34: 499-512 (2001)
26 Lee YE, Hong SH. Natural Food Material, Kyomunsa Publishers Inc., Seoul, Korea. pp. 150-151 (2003)
27 Lee YS, Yoo JM, Shin HW, Kim DH, Lee YM, Yun YP, Hong JT, Oh S, Yoo HS. Protective effect of ginsenoside Rg1 on $H_2O_2$-induced cell death by the decreased ceramide level in LLC-PK1 cells. J. Ginseng Res. 30: 1-7 (2006)   과학기술학회마을   DOI
28 Kim YC, Yim JH, Rho JH, Cho CW, Rhee YK. Antioxidant activity of white ginseng extracts prepared by enzyme treatment on V79-4 cells induced by oxidative stress. J. Ginseng Res. 31: 203-209 (2007)   과학기술학회마을   DOI
29 Chung MJ, Kang AY, Lee KM, Oh E, Jun HJ, Kim SY, Auh JH, Moon TW, Lee SJ, Park KH. Water-soluble genistin glycoside isoflavones up-regulate antioxidant metallothionein expression and scavenge free radicals. J. Agr. Food Chem. 54: 3819-3826 (2006)   DOI   ScienceOn
30 Ko MS, Shin KM, Lee MY. Effects of Hijikia fusiforme ethanol extract on antioxidative enzymes in ethanol-induced hepatotoxicity of rat liver. J. Korean Soc. Food Sci. Nutr. 31: 87-91 (2002)   과학기술학회마을   DOI
31 Han EG, Sung IS, Moon HG, Cho SY. Effects of Codonopsis lanceolata water extract on the level of lipid in rats fed high fat diet. Korean J. Soc. Food Sci. Nutr. 27: 940-944 (1998)
32 Oh MH, Chung HY, Yang HS, Kim KW, Hikokichi O, Takako Y. Effects of ginsenoside $Rb_2$ on the antioxidants in SAM-R/1 mice. Korean Biochem. J. 25: 492-497 (1992)
33 Block G, Langseth L. Antioxidant vitamins and disease prevention. Food. Technol. 48(7): 80-84 (1994)
34 Harman D. Free radical theory of aging. Mutat. Res. 275: 257-266 (1992)   DOI