• Journal of Ginseng Research
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• v.41 no.3
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• pp.268-276
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• 2017

Background: UV-B-exposed keratinocytes secrete various paracrine factors. Among these factors, basic fibroblast growth factor (bFGF) stimulates the proliferation of melanocytes. Ginsenosides, the major active compounds of ginseng, are known to have broad pharmacological effects. In this study, we examined the antiproliferative effects of ginsenosides on bFGF-induced melanocyte proliferation. Methods: We investigated the inhibitory effects of Korean Red Ginseng and ginsenosides from Panax ginseng on bFGF-induced proliferation of melan-a melanocytes. Results: When melan-a melanocytes were treated with UV-B-irradiated SP-1 keratinocytes media, cell proliferation increased. This increased proliferation of melanocytes decreased with a neutralizing anti-bFGF antibody. To elucidate the effects of ginsenosides on melanocyte proliferation induced by bFGF, we tested 15 types of ginsenoside compounds. Among them, Rh3, Rh1, F1, and CK demonstrated antiproliferative effects on bFGF-induced melanocyte proliferation after 72 h of treatment. bFGF stimulated cell proliferation via extracellular signal-regulated kinase (ERK) activation in various cell types. Western blot analysis found bFGF-induced ERK phosphorylation in melan-a. Treatment with Rh3 inhibited bFGF-induced maximum ERK phosphorylation and F1-delayed maximum ERK phosphorylation, whereas Rh1 and CK had no detectable effects. In addition, cotreatment with Rh3 and F1 significantly suppressed bFGF-induced ERK phosphorylation. Western blot analysis found that bFGF increased microphthalmia-associated transcription factor (MITF) protein levels in melan-a. Treatment with Rh3 or F1 had no detectable effects, whereas cotreatment with Rh3 and F1 inhibited bFGF-induced MITF expression levels more strongly than a single treatment. Conclusion: In summary, we found that ginsenosides Rh3 and F1 have a synergistic antiproliferative effect on bFGF-induced melan-a melanocyte proliferation via the inhibition of ERK-mediated upregulation of MITF.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.16 no.2
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• pp.59-70
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• 2012

Objectives : Pulse diagnosis by Expert is the only way to classify 8 Constitutions so the study to supplement classifying method by the questionnaire has developed and modified and ECM-32 System has designed in 2010. But analyzing with Decision tree had many nodes and 32 important questions omitted while processing the data. So this study was to classify the 8 constitution patients into 2 groups first and analyze its characters in consecutive order. Methods : The participants of this study were 1027 patients who classified into one of the 8 constitutions according to pulse diagnosis and answered 251 questionnaires in 2010. They were divided into sympathetic nerve acceleration constitution and parasympathetic nerve acceleration constitution and analyzed with decision tree. Results : The reponses of the questionnaire were analyzed with 4 methods of 5 scales interval method from 0 to 5, Na, Low(1,2), Medium(3), High(4,5), average value, Y/N dichotomy. Average Value had no significance. 1. From the 5 scale interval method 6 questionnaires with 7 nodes (F5e, B1d, F7f, F2a, F1b, C4L) were significant. The accuracy was 92.5%. 2. From L, M, H method 7 questionnaires with 7 nodes(F5e, B1d, F7f, F1a, B1c, C4L, P3d) were significant. The accuracy was 92.5%. 3. From Y/N dichotomy 9 questionnaires with 9 nodes( F5e, B1d, F7f, F1a, B1c, C4L, B1b, P1i, B2a) were significant. The accuracy was 93.18%. Conclusions : Based on this study, Yes or No dichotomy method was most significant and categorized among the 4 methods. Unlike previous studies which used interval scale method only, Y/N dichotomy method was more statistically significant with the questionnaire to supplement the method of pulse diagnosis. For further study by analyzing decision tree method in consecutive order, the patients can be divided into 8 Constitutions with higher significance with less questionnaires.

• Korean Journal of Mathematics
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• v.25 no.4
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• pp.483-494
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• 2017

In the current manuscript, we investigate the pointwise convergence of the singular integral operators involving power nonlinearity given in the following form: $$T_{\lambda}(f;x)={\int_a^b}{\sum^n_{m=1}}f^m(t)K_{{\lambda},m}(x,t)dt,\;{\lambda}{\in}{\Lambda},\;x{\in}(a,b)$$, where ${\Lambda}$ is an index set consisting of the non-negative real numbers, and $n{\geq}1$ is a finite natural number, at ${\mu}$-generalized Lebesgue points of integrable function $f{\in}L_1(a,b)$. Here, $f^m$ denotes m-th power of the function f and (a, b) stands for arbitrary bounded interval in ${\mathbb{R}}$ or ${\mathbb{R}}$ itself. We also handled the indicated problem under the assumption $f{\in}L_1({\mathbb{R}})$.

• Bulletin of the Korean Mathematical Society
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• v.54 no.6
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• pp.1981-1990
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• 2017

Let R be a root system in $\mathbb{R}^d$ with Coxeter-Weyl group W and let k be a nonnegative multiplicity function on R. The generalized volume mean of a function $f{\in}L^1_{loc}(\mathbb{R}^d,m_k)$, with $m_k$ the measure given by $dmk(x):={\omega}_k(x)dx:=\prod_{{\alpha}{\in}R}{\mid}{\langle}{\alpha},x{\rangle}{\mid}^{k({\alpha})}dx$, is defined by: ${\forall}x{\in}\mathbb{R}^d$, ${\forall}r$ > 0, $M^r_B(f)(x):=\frac{1}{m_k[B(0,r)]}\int_{\mathbb{R}^d}f(y)h_k(r,x,y){\omega}_k(y)dy$, where $h_k(r,x,{\cdot})$ is a compactly supported nonnegative explicit measurable function depending on R and k. In this paper, we prove that for almost every $x{\in}\mathbb{R}^d$, $lim_{r{\rightarrow}0}M^r_B(f)(x)= f(x)$.

• Journal of the Chungcheong Mathematical Society
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• v.27 no.3
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• pp.489-494
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• 2014

In this paper, we define the extension $f^*:[a,b]{\rightarrow}\mathbb{R}$ of a function $f:[a,b]_{\mathbb{T}}{\rightarrow}\mathbb{R}$ for a time scale $\mathbb{T}$ and investigate the properties of the Lebesgue delta integral of f on $[a,b]_{\mathbb{T}}$ by using the function $f^*$.

• Asian Journal of Atmospheric Environment
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• v.6 no.2
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• pp.73-82
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• 2012

This study was aimed to thoroughly estimate the characteristics of indoor particulate matter (PM) collected on subway platforms by the cooperative approach of semi-bulk and single particle analyses. The size-resolved PM and its number concentration were measured on the platform in a heavily traveled subway station in Fukuoka, Japan. Particle Induced X-ray Emission (PIXE) and micro-PIXE techniques were applied to the chemical analyses of semi-bulk and single particle, respectively. There was the close resemblance of timely fluctuation between PM number concentration and train service on the third basement floor (B3F) platform compared to the second basement floor (B2F) and its maximum level was marked in rush hour. Higher number counts in large particles ($>1{\mu}m$) and lower number counts in fine particles ($<1{\mu}m$) were shown on the platform compared to an above ground. PM2.5 accounted for 58.2% and 38.2 % of TSP on B3F and on B2F, respectively. The elements that were ranked at high concentration in size-resolved semi-bulk PM were Fe, Si, Ca, S, and Na. The major elements tending to have more elevated levels on B3F than B2F were Fe (4.4 times), Ca (17.3 times), and Si (46.4 times). Although concentrations were very low, Cr ($11.9ng\;m^{-3}$ on B3F, $2.4ng\;m^{-3}$ on B2F), Mn ($3.4ng\;m^{-3}$ on B3F, $0.9ng\;m^{-3}$ on B2F), and Pb ($0.6ng\;m^{-3}$ on B3F, $1.6ng\;m^{-3}$ on B2F) were detected from PM2.5. Individual PM was nearly all enriched in Fe with Si and Ca. Classifying and source profiling of the individual particles by elemental maps and particle morphology were tried and particles were presumably divided into four groups (i.e., train/rail friction, train-rail sparking, ballast/abrasive, and cement).

• Bulletin of the Korean Mathematical Society
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• v.49 no.4
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• pp.875-883
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• 2012

Let $\mathbb{F}_q$ be a finite field with q elements and $\mathbb{F}_q((X^{-1}))$ be the field of all formal Laurent series with coefficients lying in $\mathbb{F}_q$. This paper concerns with the size of the set of points $x{\in}\mathbb{F}_q((X^{-1}))$ with their partial quotients $A_n(x)$ both lying in a given subset $\mathbb{B}$ of polynomials in $\mathbb{F}_q[X]$ ($\mathbb{F}_q[X]$ denotes the ring of polynomials with coefficients in $\mathbb{F}_q$) and deg $A_n(x)$ tends to infinity at least with some given speed. Write $E_{\mathbb{B}}=\{x:A_n(x){\in}\mathbb{B},\;deg\;A_n(x){\rightarrow}{\infty}\;as\;n{\rightarrow}{\infty}\}$. It was shown in [8] that the Hausdorff dimension of $E_{\mathbb{B}}$ is inf{$s:{\sum}_{b{\in}\mathbb{B}}(q^{-2\;deg\;b})^s$ < ${\infty}$}. In this note, we will show that the above result is sharp. Moreover, we also attempt to give conditions under which the above dimensional formula still valid if we require the given speed of deg $A_n(x)$ tends to infinity.

• The Plant Pathology Journal
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• v.32 no.1
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• pp.53-57
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• 2016

Major diseases in grafted cacti have been reported and Fusarium oxysporum, Bipolaris cactivora, Phytophthora spp. and Collectotrichum spp. are known as causal pathogens. These pathogens can lead to plant death after infection. Therefore, some European countries have quarantined imported cacti that are infected with specific fungal pathogens. Consequently, we developed PCR detection methods to identify four quarantined fungal pathogens and reduce export rejection rates of Korean grafted cacti. The pathogen specific primer sets F.oF-F.oR, B.CF-B.CR, P.nF-P.nR, and P.cF-P.CR were tested for F. oxysporum, B.cactivora, P. nicotinae, and P. cactorum, respectively. The F.oF-F.oR primer set was designed from the Fusarium ITS region; the B.CF-B.CR and P.nF-P.nR primers respectively from Bipolaris and Phytophthora ITS1; and the P.cF-P.CR primer set from the Ypt1protein gene region. The quarantine fungal pathogen primer pairs were amplified to the specific number of base pairs in each of the following fungal pathogens: 210-bp (F. oxysporum), 510-bp (B. cactivora), 313-bp (P. nicotinae), and 447-bp (P. cactorum). The detection limit for the mono- and multiplex PCR primer sets was 0.1 ng of template DNA under in vitro conditions. Therefore, each primer set successfully diagnosed contamination of quarantine pathogens in export grafted cacti. Consequently, our methodology is a viable tool to screen contamination of the fungal pathogen in exported grafted cacti.

• Journal of Life Science
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• v.24 no.9
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• pp.1001-1005
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• 2014

Ginsenoside Rg3 is one of the active ingredients extracted from red ginseng, and it is an effective chemical component of the human body and well known in herbal medicine as a restorative agent. Several studies have shown that Rg3 has a potent anti-tumor effect on various cancer cell lines. However, Rg3-induced apoptosis in B16F10 melanoma cancer cells is not well understood. In the present study, we tested whether ginsenoside Rg3 could induce apoptosis in B16F10 melanoma cells. We found that Rg3 could inhibit B16F10 melanoma cell viability in a dose-dependent manner, but not normal cells, such as EA.hy.926 and NIH3T3 cells. We also found that Rg3 could induce apoptosis in B16F10 melanoma cells using tunnel-staining assay in a dose-dependent manner. Rg3 treatment induces the phosphorylation of p38 and the expression of Bax, but it inhibits the expressions of the phosphorylation of focal adhesion kinase Bcl2 and pro-caspase3. Taken together, our data suggest that Rg3 could be useful as an anti-cancer agent in B16F10 melanoma cells.

• Korean Journal of Mathematics
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• v.7 no.2
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• pp.183-198
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• 1999

Let $\mathcal{F}(B)$ be the Fresnel class on an abstract Wiener space (B, H, ${\omega}$) which consists of functionals F of the form : $$F(x)={\int}_H\;{\exp}\{i(h,x)^{\sim}\}df(h),\;x{\in}B$$ where $({\cdot}{\cdot})^{\sim}$ is a stochastic inner product between H and B, and $f$ is in $\mathcal{M}(H)$, the space of all complex-valued countably additive Borel measures on H. We introduce the concepts of an $L_p$ analytic Fourier-Feynman transform ($1{\leq}p{\leq}2$) and a convolution product on $\mathcal{F}(B)$ and verify the existence of the $L_p$ analytic Fourier-Feynman transforms for functionls in $\mathcal{F}(B)$. Moreover, we verify that the Fresnel class $\mathcal{F}(B)$ is closed under the $L_p$ analytic Fourier-Feynman transform and the convolution product, respectively. And we investigate some interesting properties for the $n$-repeated $L_p$ analytic Fourier-Feynman transform on $\mathcal{F}(B)$. Finally, we show that several results in [9] come from our results in Section 3.