Browse > Article
http://dx.doi.org/10.5352/JLS.2014.24.9.1001

Ginsenoside Rg3 Induces Apoptosis in B16F10 Melanoma Cells  

Lee, Seul Gi (Department of Ecological Environment, Kyungbook National University)
Kim, Byung Soo (Department of Ecological Environment, Kyungbook National University)
Nam, Ju-Ock (Department of Ecological Environment, Kyungbook National University)
Publication Information
Journal of Life Science / v.24, no.9, 2014 , pp. 1001-1005 More about this Journal
Abstract
Ginsenoside Rg3 is one of the active ingredients extracted from red ginseng, and it is an effective chemical component of the human body and well known in herbal medicine as a restorative agent. Several studies have shown that Rg3 has a potent anti-tumor effect on various cancer cell lines. However, Rg3-induced apoptosis in B16F10 melanoma cancer cells is not well understood. In the present study, we tested whether ginsenoside Rg3 could induce apoptosis in B16F10 melanoma cells. We found that Rg3 could inhibit B16F10 melanoma cell viability in a dose-dependent manner, but not normal cells, such as EA.hy.926 and NIH3T3 cells. We also found that Rg3 could induce apoptosis in B16F10 melanoma cells using tunnel-staining assay in a dose-dependent manner. Rg3 treatment induces the phosphorylation of p38 and the expression of Bax, but it inhibits the expressions of the phosphorylation of focal adhesion kinase Bcl2 and pro-caspase3. Taken together, our data suggest that Rg3 could be useful as an anti-cancer agent in B16F10 melanoma cells.
Keywords
Anticancer; apoptosis; B16F10; ginsenoside Rg3; malignant melanoma cancer;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Cheong, J. H., Hong, E. Y., Kim, H. J., Park, I. H., Park, S., Pena, I. D., Ryu, J. H. and Yoon, S. Y. 2013. Effects of ginseol k-g3, an Rg3-enriched fraction, on scopolamine- induced memory impairment and learning deficit in mice. J Ginseng Res 38, 1-7.   DOI
2 Chun, J. M., Ha, Y. M., Han, Y., Joo, E. J., Kim, J. S., Kim, Y. S. and Lee, J. H. 2012. Induction of Apoptosis by Ginsenoside Rk1 in SK-MEL-2-Human Melanoma. Arch Pharm Res 35, 717-722.   DOI   ScienceOn
3 Cho, J. S., Choi, S. S., Han, S. B., Hong, J. T., Kim, S. M., Lee, S. Y., Oh, K. W., Son, S. M., Yoon, do. Y., Yoo, H. S. and Yun, Y. P. 2010. Combination of ginsenoside Rg3 with docetaxel enhances the susceptibility of prostate cancer cells via inhibition of NF-kappaB. Eur J Pharmacol 631, 1-9.   DOI   ScienceOn
4 Choi, K. C., Choi, W. I., Ham, J., Jang, H. J., Jeon, B. N., Kang, K. S., Kim, K., Kim, T. J., Kim, Y. J. and Ko, H. 2014. Stereospecific effects of ginsenoside 20-Rg3 inhibits TGF-${\beta}$ 1-induced epithelial-mesenchymal transition and suppresses lung cancer migration, invasion and anoikis resistance. Toxicology 322, 23-33.   DOI
5 Choi, S. Y., Eom, D. W., Ham, J. Y., Han, D. J., Jang, H. J., Kang, K. S., Kim, S. S., Kim, Y. H., Kim, Y. J., Lee, J. H., Oh, M. Y. and Wee, Y. M. 2014. Ginsenoside rg3 enhances islet cell function and attenuates apoptosis in mouse islets. Transplant Proc 46, 1150-1155   DOI
6 Dandin, V. S., Lee, E. J., Murthy, H. N. and Paek, K. Y. 2014. Efficacy of ginseng adventitious root extract on hyperglycemia in streptozotocin-induced diabetic rats. J Ethnopharmacol 153, 917-921.   DOI
7 Fang, J. P., Lao, C. H., Wang, J. C., Xia, T., Xu, L. H., Xu, W. and Ye, Q. X. 2014. 20S-Ginsenoside Rh2 induces apoptosis in human Leukaemia Reh cells through mitochondrial signaling pathways. Biol Pharm Bull 37, 248-54.   DOI
8 Hai, Y. N., Nan, K. J., Nie, Y. L., Sun, H. F., Wang, S. H., Wang, Y. C. and Yuan, Z. L. 2013. Antiproliferative Activity of the Chinese Medicinal Compound, Delisheng, Copa-pared With Rg3 and Gemcitabine in HepG2 Cells. Indian J Pharm Sci 75, 578-584
9 Jin, Y., Kim, Y. J., Quan, L. H., Wang, C. and Wang, T. R. and Yang, D. C. 2013. Isolation and characterization of novel ginsenoside-hydrolyzing glycosidase from Microbacterium esteraromaticum that transforms ginsenoside Rb2 to rare ginsenoside 20(S)-Rg3. Antonie Van Leeuwenhoek 104, 129-137.   DOI   ScienceOn
10 Jung, E. Y., Kim, J. W., Kwon, S. M., Kwon, Y. H., Lee, B. Y., Lee, J. H. and Lee, Y. M. 2012. Ginsenoside Rg3 attenuates tumor angiogenesis via inhibiting bioactivities of endothelial progenitor cells. Cancer Bio Ther 13, 504-515.   DOI   ScienceOn
11 Kang, H. S., Kim, J. S., Kim, S. J. and Park, H. M. 2012. Reactive oxygen species mediated ginsenoside Rg3 and Rh2-induced apoptosis in hepatoma cells through mitochondrial signaling pathways. Food Chem Toxicol 50, 2736-2741   DOI   ScienceOn
12 Chang, K. H., Jo, M. N., Kim, K. T. and Paik, H. D. 2014. Evaluation of glucosidases of Aspergillus niger strain comparing with other glucosidases in transformation of ginsenoside Rb1 to ginsenosides Rg3. J Ginseng Res 38, 47-51.   DOI
13 Chen, J., He, X., Peng, H. and Yang, X. O. 2008. Research on the antitumor effect of ginsenoside Rg3 in B16 melanoma cells. Melanoma Res 18, 322-329   DOI   ScienceOn
14 Cai, J., Cao, Y., Cheng, H., Ge, X., Ma, J., Sun, X., Yang, B., Yang, X., Zhang, C., Zhang, S. and Zhen, F. 2014. Ginsenoside Rg3 enhances radiosensitization of hypoxic oesophageal cancer cell lines through vascular endothelial growth factor and hypoxia inducible factor 1${alpha}$. J Int Med Res 42, 628-640.   DOI