• Korean Chemical Engineering Research
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• 제48권6호
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• pp.700-703
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• 2010

본 연구에서는 두충나무, 황벽나무 등을 포함하는 수목추출물의 소재특성을 시험하여 화장품 및 피부질환 치료소재로서의 응용 가능성을 조사하였다. 수목추출물의 항산화력은 DPPH 자유라디칼 소거법으로 측정되었으며 저농도에서는 항산화력이 낮았으나, $1000{\mu}g/ml$의 고농도에서는 우수한 항산화제인 비타민 C와 유사하였다. 항균력은 원판확산법으로 측정되었으며, 수목추출물은 피부상재균인 Staphylococcus epidermidis에 대해서는 낮은 항균력을 보이지만, 여드름 유발균인 Propionibacterium acne에 대해서는 높은 항균력을 보여주는 선택성 항균력이 있어, 여드름소재로서 매우 우수한 특성을 나타내었다. 무모생쥐를 이용한 항아토피 시험결과, 수목추출물은 DNCB에 의해 유도된 피부염증을 14일 째 거의 정상상태로 회복시켰으며, 피부내 항체인 IgE의 농도도 대조군에 비해 16% 낮추어주어 항아토피 효과가 우수한 것으로 나타났다. 따라서 수목추출물은 항여드름효과 및 항아토피 효과가 뛰어나서 화장품 혹은 피부질환 치료소재로서 응용 가능성이 매우 높음을 알 수 있었다.

• 한국균학회지
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• 제26권4호통권87호
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• pp.502-510
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• 1998

장수버섯 자실체로부터 분리된 강력한 항보체 활성 다당체인 FF-AP1을 정제하고 이것의 화학적 특성을 구명하기 위해 본 실험을 수행한 결과는 다음과 같다. ConA-Sepharose 4B affinity chromatography에 의해 FF-AP1은 $FF-AP1{\alpha}$와 $FF-AP1{\beta}$로 더 분리되었으며 이 중 항보체 활성의 주 유효 다당체인 $FF-AP1{\beta}$는 $10\;{\mu}g/ml$ 농도에서도 약 70%의 항보체 활성도를 나타내었다. $FF-AP1{\beta}$는 약 9.6%의 단백질을 함유한 산성다당체이며 glutamic acid와 aspartic acid의 함량이 상대적으로 다른 아미노산들에 비해 높았다. 이것의 분자량은 gel filtration법에 의해 15,000인 것으로 추정되었고 적외선 분광 분석에서 $890cm^{-1}$ 부근의 소peak를 탐지함으로써 ${\beta}$ glucosidic 결합의 존재를 확인하였다. Gas chromatography에 의한 구성당 조성 분석 결과, 주 구성당이 glucose, mannose, 및 galactose인 galacto-manno-glucan이었고, 소량의 rhamnose, xylose, fucose와 uronic acid도 탐지되었다.

• 대한화장품학회지
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• 제32권3호
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• pp.193-200
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• 2006

화장품 원료로서 황기추출물을 산지 및 추출방법에 따라서 추출한 후, 보습효과 및 항산화효과를 시험하였다. 황기는 산지별로서 제천산, 정선산, 영주산, 태백산 및 중국산 1년 근을 사용하였다. 황기추출물에 포함된 유효성분의 한 지표성분으로서 선정한 formononetin을 HPLC 분석법을 이용하여 분석하였다. 항산화능 평가로서 자유라디칼 및 활성산소 소거효과의 경우에 75% 에탄을 수용액을 이용한 황기추출물은 정제수를 이용한 황기추출물과 비교하여 더 우수한 것으로 나타났다. 75% 에탄을 추출물의 자유라디칼 소거효과는 50% 소거효율에 대한 농도($IC_{50}$)가 2.162 mg/mL이었으며, 활성산소 소거효과의 경우는 $IC_{50}$ 값이 2.981 mg/mL로 나타났다. 특히 에틸아세테이트 분획층에 포함되어 있는 이소플라보노이드는 항산화제로 알려진 제니스테인과 유사한 자유라디칼 소거효과를 나타냈다. 황기추출물의 보습효과는 제천산 황기추출물이 가장 양호하였으며, 초음파추출을 이용한 황기추출물이 상대적으로 양호하였다.

• 동의생리병리학회지
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• 제23권1호
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• pp.135-143
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• 2009

There is currently increased interest in the identification of antioxidant compounds that are pharmacologically potent and have low or no side effects. Plants produce significant amounts of antioxidants to prevent the oxidative stress caused by photons and oxygen, therefore they represent a potential source of new compounds with antioxidant activity. Moutan Root Sark (MRS) has been frequently used as analgesic. antispasmodic, anti-inflammatory and remedies for female diseases. In this study. the antioxidant activity of extract from MRS was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$ induced human LDL oxidation and the inhibitory effect on collagen induced platelet aggregation. The MRS extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on platelet aggregation. In conclusion, the MRS extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.

• 대한본초학회지
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• 제31권3호
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• pp.59-69
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• 2016

Objectives： Presently Mume Fructus (MF) undergoes fumigation, which produces benzo[a]pyrene. As a primary analysis with the aims to minimize the production of benzo[a]pyrene and to suggest standards for processing the MF, the steaming method was chosen among the various processing methods, and reviewed through a series of experiments.Methods： Methods：Pitted and un-pitted MF were steamed and processed into samples. After testing level of benzo[a]pyrene, the samples were analyzed for amount of polyphenol and flavonoids. Scavenging activities of the samples for the DPPH and ABTS radicals were tested. In order to measure anti-inflammatory effects of the samples, cell survival rate was investigated using CCK-8 Assay. Also, water extracts of dried and steamed MF were administered to the RAW 264.7 cells to compare expressions of NO, PGE₂, IL-1β, and TNF-α. In addition, anti-diarrhea effects of the herbal medicine were tested on animal models with diarrhea induced by MgSO₄ and Castor oil.Results： Regardless of pitting, processed MF contained no benzo[a]pyrene. Anti-oxidation effect increased in relation to the frequency of steaming process. However, extracts of dried and steamed MF suppressed different kinds of inflammation factors, and extract of dried MF showed superior anti-diarrhea effect than extract of steamed MF.Conclusions： It is suggested that steaming method of MF is recommended for processing the herbal medicine without the production of benzo[a]pyrene. But regarding that dried and steamed MF showed differences in their anti-oxidative, anti-inflammatory, and anti-diarrhea effects, it is recommended to perform further researches on different efficacies of MF according to their processing methods.

• 한국식품저장유통학회지
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• 제13권1호
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• pp.83-87
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• 2006

한의학에서 천마는 여러 치료의 목적으로 사용이 되는 한약재이다. 본 연구는 천마의 항산화활성과 항암 효과를 검증하기 위하여 실시하였다. 천마와 이를 이용한 식음료를 이용하여 항산화활성과 암세포를 이용한 세포의 운동성을 측정하였다. 항산화활성의 경우 DPPH, FRAP, Hydroxyl radical assay를 실시하였으며, 항암효과를 확인하기 위하여 wound / invasion assay를 실시하였다. 실험 결과 천마의 항산화 활성은 매우 뛰어난 것으로 나타났으며, 특히 DPPH / FRAP 실험에서 높은 활성을 보여주었다. 항암효과로 세포의 운동성을 확인한 결과 천마와 제품군 모두에서 세포의 운동성을 억제하는 효과가 매우 높은 것으로 나타났다. 천마는 항산화 효과와 함께 암세포의 운동성을 억제하는 항암효과를 가지는 것으로 나타났다.

• Applied Biological Chemistry
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• 제51권3호
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• pp.183-187
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• 2008

본 연구에서는 마늘 중의 항미생물 작용을 가지고 있는 allyl-2-propenyl-1-thiosulfinate의 유사체들인 alkyl thiosulfinate 및 이의 산화물인 thiosulfonate화합물들을 합성하고 이들의 생물 활성을 검사하였다. Alkylsulfinate는 이황화 화합물(disulfide)를 유기 과산화산으로, 또 alkyl thiosulfonate는 thiosulfinite를 sodium periodate로 산화시켜 합성하였다. 합성한 모든 alkylthiosulfinate 및 alkyl thiosulfonate들은 Staphylococcus aureus B33에 대해서는 항세균성을, Candida utilis ATCC42416에 대해서는 항곰팡이성을 나타내었다. 나아가 이들 화합물들은 항산화성과 항응고 활성도 나타내었다.

• 대한본초학회지
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• 제30권4호
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• pp.89-94
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• 2015

Objectives : It had been reported that herbal medicines containing polyphenol and flavonoid have been shown to be associated with decreased the cause of aging and variety of disease such as reactive nitrogen species and reactive oxygen species in several recent studies. Salviae miltiorrhizae Radix, origined fromSalvia miltiorrhizaBGE., is one of popular traditional herbal medicines that is commonly used by traditional medicine practitioners. To this date, Salviae miltiorrhizae Radix has more than 2000-year history of mature application. This study was conducted to investigate whether the Salviae miltiorrhizae Radix methanol extract has an inhibitory effect association with oxidation or inflammation.Methods : Cytotoxic activity of Salviae miltiorrhizae Radix methanol extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide solution. Nitric oxide production was measured using griess reagent system. Western blot analysis and measurement for changes of protein expression, nitric oxide synthase and cyclooxygenase-2, also performed.Results : The medicinal plant, Salviae miltiorrhizae Radix, does not impair the cell viability in tested concentration (25-100 μg/ml). SMR showed anti-oxiative effectsin vitroby decreasing electron donating ability, and also showed anti-inflammatory effects suppressing NO and COX-2 expressin in LPS induced RAW264.7 activation. SMR inhibited the generation of intracellular ROS production as dose dependant manner.Conclusions : These results indicate that Salviae miltiorrhizae Radix methanol extract has an anti-inflammatory activities via anti-oxidative effects, and the anti-inflammatory effect was presentedd as dose dependant manner.

• 한국한의학연구원논문집
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• 제14권1호
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• pp.49-58
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• 2008

Objective : To provide information about the accessory product of Broussonetia kazinoki Siebold or Broussonetia papyrifera (L.) Vent by analyzing old books of oriental medicine, domestic/international papers and related patents Methods : Old books related to the accessory product in the field of oriental medicine were reviewed. Research papers regarding the pharmacological activity of the by-products were reviewed and analyzed. Patents about the residual products were examined and classified by year and subject Results : Seven kinds of by-products from Broussonetia kazinoki Siebold or Broussonetia papyrifera (L.) Vent has been used as medicines in oriental medicine. Recently, anti-oxidating, anti-cancer, anti-mutagenic and anti-inflammation activity of the residual product of these plants has been investigated through scientific research. There were 19 patents related with the accessory products of these plants, which were in the subjects of functional cosmetics, anti-inflammation, cleansing goods, hair restorers or improvement of learning ability. Further investigations about the activity of these plants are needed in bone metabolism, water balance and hemostasis in the future. Conclusion : Residual products from these plants is being used in various ways. However, more studies on the efficacy and mechanism, as well as safety, of these plants should be conducted precisely in the future.

• 한방재활의학과학회지
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• 제25권4호
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• pp.1-20
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• 2015

Objectives This study was intended to clarify the anti-inflammation and anti-oxidation effects of gamikyejakjimo-tang herbal acupuncture (GKHA) for osteoarthritis. Methods Osteoarthritis was induced by injection of MIA into right knee joint cavities of rats. Rats were divided into a total of 4 groups (n=8). The 4 groups were normal group, control group, positive comparison group and expeimental group. Indomethacin and GKHA were medicated for a total of 4 weeks. After that, functions of liver and kidney by AST, ALT, creatinine, BUN, DPPH and ABTS free radical scavenging activity, ROS (reactive oxygen species) production, NO (Total Nitric oxide), IL-$1{\beta}$, IL-6, TNF-${\alpha}$ production, weight changes in the hind legs of MIA-induced osteoarthritis rat, serum PGE2, TIMP-1, MMP-2, MMP-9, LTB4, hs-CRP, and white blood cells, neutrophils, lymphocytes, monocytes were measured. The volume of cartilage was observed by micro CT arthrography. H&E and Safranin-O staining were used to examine the injury of synovial tissue. Results 1. In the hind leg weight bearing measurement, level of weight was increased. 2. AST, ALT, BUN, creatinine were decreased. 3. The production of total white blood cell was decreased, and the production of neutrophils, lymphocytes, monocytes were significantly decreased. 4. The production of NO, PGE2, TIMP-1, MMP-2, MMP-9, LTB4 were significantly decreased, and the production of hs-CRP was also decreased but with no significance. 5. The cartilage volume was significantly increased. 6. In H&E staining and Safranin-O staning, the cartilage cell appeared to be proliferated, and proteoglycans appeared to be increased. Conclusions Based on the results above, Gamikyejakjimo-tang Herbal Acupuncture has anti-oxidation and anti-inflammation effects, which leads to suppressing the underlying causes and the progression of osteoarthritis.