Browse > Article
http://dx.doi.org/10.6116/kjh.2015.30.4.89.

Anti-inflammatory effects of Salviae Miltiorrhizae Radix extract on RAW264.7 cell. via anti-oxidative activities  

Lee, Se-Eun (school of Korean Medicine, Pusan National University)
Cho, Su-In (School of Korean Medicine, Pusan National University)
Publication Information
The Korea Journal of Herbology / v.30, no.4, 2015 , pp. 89-94 More about this Journal
Abstract
Objectives : It had been reported that herbal medicines containing polyphenol and flavonoid have been shown to be associated with decreased the cause of aging and variety of disease such as reactive nitrogen species and reactive oxygen species in several recent studies. Salviae miltiorrhizae Radix, origined fromSalvia miltiorrhizaBGE., is one of popular traditional herbal medicines that is commonly used by traditional medicine practitioners. To this date, Salviae miltiorrhizae Radix has more than 2000-year history of mature application. This study was conducted to investigate whether the Salviae miltiorrhizae Radix methanol extract has an inhibitory effect association with oxidation or inflammation.Methods : Cytotoxic activity of Salviae miltiorrhizae Radix methanol extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide solution. Nitric oxide production was measured using griess reagent system. Western blot analysis and measurement for changes of protein expression, nitric oxide synthase and cyclooxygenase-2, also performed.Results : The medicinal plant, Salviae miltiorrhizae Radix, does not impair the cell viability in tested concentration (25-100 μg/ml). SMR showed anti-oxiative effectsin vitroby decreasing electron donating ability, and also showed anti-inflammatory effects suppressing NO and COX-2 expressin in LPS induced RAW264.7 activation. SMR inhibited the generation of intracellular ROS production as dose dependant manner.Conclusions : These results indicate that Salviae miltiorrhizae Radix methanol extract has an anti-inflammatory activities via anti-oxidative effects, and the anti-inflammatory effect was presentedd as dose dependant manner.
Keywords
Salviae Miltiorrhizae Radix; Anti-oxidation; Anti-inflammatory; RAW264.7;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Sies H. Oxidative stress: From basic research to clinical application. Am J Med. 1991 ; 91(3C) : 31S-8S.   DOI   ScienceOn
2 Guo Y, Li Y, Xue L, Severino RP, Gao S, Niu J, Qin LP, Zhang D, Brömme D. Salvia miltiorrhiza: An ancient Chinese herbal medicine as a source for anti-osteoporotic drugs. J Ethnopharmacol. 2014 ; 155(3) : 1401-16.   DOI   ScienceOn
3 Li D, Xiong SD, Du DB. Inhibitory effects of Salvia miltiorrhiza injection coordinated with dexamethasone on interleukin-13 and eotaxin expression in lung of asthmatic rats. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2006 ; 26(11) : 1007-10.
4 Liu QS, Luo XY, Jiang H, Xing Y, Yang MH, Yuan GH, Tang Z, Wang H. Salvia miltiorrhiza injection restores apoptosis of fibroblast-like synoviocytes cultured with serum from patients with rheumatoid arthritis. Mol Med Rep. 2015 ; 11(2) : 1476-82.   DOI
5 Wen XD, Wang CZ, Yu C, Zhang Z, Calway T, Wang Y, Li P, Yuan CS. Salvia miltiorrhiza (dan shen) significantly ameliorates colon inflammation in dextran sulfate sodium induced colitis. Am J Chin Med. 2013 ; 41(5) : 1097-108.   DOI   ScienceOn
6 Gheldof N, Engeseth NJ. Antioxidant capacity of honeys from various floral sources based on the determination of oxygen radical absorbance capacity and inhibition of in vitro lipoprotein oxidation in human serum samples. J Agric Food Chem. 2002 ; 50(10) : 3050-5.   DOI   ScienceOn
7 Wu YJ, Hong CY, Lin SJ, Wu P, Shiao MS. Increase of Vitamin E Content in LDL and Reduction of Atherosclerosis in Cholesterol-Fed Rabbits by a Water-Soluble Antioxidant-Rich Fraction of Salvia miltiorrhiza. Arterioscler Thromb Vasc Biol. 1998 ; 18(3) : 481-6.   DOI   ScienceOn
8 Lee ES, Ju HK, Moon TC, Lee EK, Jahng YD, Lee SH, Son JK, Baek SH, Chang HW. Inhibition of nitric oxide and tumor necrosis factor-alpha (TNF-alpha) production by propenone compound through blockade of nuclear factor (NF)-kappa B activation in cultured murine macrophages. Biol Pharm Bull. 2004 ; 27(5) : 617-20.   DOI   ScienceOn
9 Choi HS, Seo HS, Kim SR, Choi YK, Jang BH, Shin YC, Ko SG. Anti inflammatory and anti proliferative effects of Rhus verniciflua Stokes in RAW264. 7 cells. Mol Med Rep. 2014 ; 9(1) : 311-5.   DOI
10 Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 2006 ; 160(1) : 1-40.   DOI   ScienceOn
11 Fridovich I. Superoxide dismutases. An adaptation to a paramagnetic gas. J Biol Chem. 1989 ; 264(14) : 7761-4.
12 Luk JM, Wang X, Liu P, Wong KF, Chan KL, Tong Y, Hui CK, Lau GK, Fan ST. Traditional Chinese herbal medicines for treatment of liver fibrosis and cancer: from laboratory discovery to clinical evaluation. Liver Int. 2007 ; 27(7) : 879-90.   DOI   ScienceOn
13 Bae DS, Kim YH, Pan CH, Nho CW, Samdan J, Yansan J, Lee JK. Protopine reduces the inflammatory activity of lipopolysaccharide-stimulated murine macrophages. BMB Rep. 2012 ; 45(2) : 108-13.   DOI   ScienceOn
14 Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem. 1998 ; 46(10) : 4113-7.   DOI   ScienceOn
15 Cerella C, Sobolewski C, Dicato M, Diederich M. Targeting COX-2 expression by natural compounds: a promising alternative strategy to synthetic COX-2 inhibitors for cancer chemoprevention and therapy. Biochem Pharmacol. 2010 ; 80(12) : 1801-15.   DOI   ScienceOn
16 Liang YC, Huang YT, Tsai SH, Lin-Shiau SY, Chen CF, Lin JK. Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages. Carcinogenesis. 1999 ; 20(10) : 1945-52.   DOI   ScienceOn
17 Kandaswami CE. Middleton E Jr. Free Radical Scavenging and Antioxidant Activity of Plant Flavonoids. Adv Exp Med Biol. 1994 ; 366 : 351-76.   DOI
18 Tsai SH, Lin-Shiau SY, Lin JK. Suppression of nitric oxide synthase and the down-regulation of the activation of NFkappaB in macrophages by resveratrol. Br J Pharmacol. 1999 ; 126(3) : 673-80.   DOI   ScienceOn
19 Blois MS. Antioxidant Determinations by the Use of a Stable Free Radical. Nature. 1958 ; 181(4617) : 1199-200.   DOI   ScienceOn
20 Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of radiosensitivity. Cancer Res. 1987 ; 47(4) : 943-6.
21 Kawai T, Akira S. Signaling to NF-kappaB by Toll-like receptors. Trends Mol Med. 2007 ; 13(11) : 460-9.   DOI   ScienceOn
22 Herbology Association of Korea's Colleges of Oriental Medicine. Text book of Herbology. Seoul : Younglimsa. 2000 : 419-20.
23 Medzhitov R. Origin and physiological roles of inflammation. Nature. 2008 ; 454(7203) : 428-35.   DOI   ScienceOn
24 Bonnert TP, Garka KE, Parnet P, Sonoda G, Testa JR, Sims JE. The cloning and characterization of human MyD88: a member of an IL-1 receptor related family 1. FEBS Lett. 1997 ; 402(1) : 81-4.   DOI   ScienceOn
25 Tak PP, Firestein GS. NF-kappaB: a key role in inflammatory diseases. J Clin Invest. 2001 ; 107(1) : 7-11.   DOI   ScienceOn
26 Kang CH, Choi YH, Choi IW, Lee JD, Kim GY. Inhibition of Lipopolysaccharide-Induced iNOS, COX-2, and TNF-α Expression by Aqueous Extract of Orixa Japonica in RAW 264.7 Cells via Suppression of NF-kB Activity. Trop J Pharm Res. 2011 ; 10(2) : 161-8.   DOI
27 NFRI. Manuals of quality characteristic analysis for food quality evaluation (2). Skuba : National Food Research Institute. 1990 : 61.
28 Nakanishi M, Rosenberg DW. Multifaceted roles of PGE2 in inflammation and cancer. Semin Immunopathol. 2013 ; 35(2) : 123-37.   DOI