The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Chemical Analysis of Acidic Proteo-heteroglycans with Anti-complementary Activity from the Hot-Water Extract of Fomitella fraxinea

장수버섯 자실체로부터 분리한 항보체 활성 단백다당체의 화학적 분석

  • 윤상홍 (농업과학기술원 분자유전과)
  • Published : 1998.12.30
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Abstract

The hot-water extract of fruiting bodies in Fomitella fraxinea had potent anti-complementary activities. After fractionation of water-soluble polysaccharides by DEAE-Sephadex A-25 column chromatography, major anti-complementary activity was concentrated into the FF-AP1 among three polysaccharides (FF-NP, FF-AP1, FF-AP2). FF-AP1 was fractionated into $FF-AP1{\alpha}$ and $FF-AP1{\beta}$ obtained from the adsorbed fraction and unadsorbed fraction by affinity chromatography using a ConA-sepharose 4B column, respectively. $FF-AP1{\beta}$, which exihibited the highest anti-complementary activities had an IR absorption peak of $890cm^{-1}$, and a M.W. of about 15,000 (gel filtration). Anti-complementary activity of FF-AP1 decreased greatly by pronase treatment and periodate oxidation. $FF-AP1{\beta}$ responsible for potent anti-complemenary activities of Fomitella fraxinea was an acidic protein-containing heteroglycan consisted of 48% glucose, 13% mannose, and 12% galactose as major component sugars, 9.6% protein, 6% uronic acids.

장수버섯 자실체로부터 분리된 강력한 항보체 활성 다당체인 FF-AP1을 정제하고 이것의 화학적 특성을 구명하기 위해 본 실험을 수행한 결과는 다음과 같다. ConA-Sepharose 4B affinity chromatography에 의해 FF-AP1은 $FF-AP1{\alpha}$와 $FF-AP1{\beta}$로 더 분리되었으며 이 중 항보체 활성의 주 유효 다당체인 $FF-AP1{\beta}$는 $10\;{\mu}g/ml$ 농도에서도 약 70%의 항보체 활성도를 나타내었다. $FF-AP1{\beta}$는 약 9.6%의 단백질을 함유한 산성다당체이며 glutamic acid와 aspartic acid의 함량이 상대적으로 다른 아미노산들에 비해 높았다. 이것의 분자량은 gel filtration법에 의해 15,000인 것으로 추정되었고 적외선 분광 분석에서 $890cm^{-1}$ 부근의 소peak를 탐지함으로써 ${\beta}$ glucosidic 결합의 존재를 확인하였다. Gas chromatography에 의한 구성당 조성 분석 결과, 주 구성당이 glucose, mannose, 및 galactose인 galacto-manno-glucan이었고, 소량의 rhamnose, xylose, fucose와 uronic acid도 탐지되었다.

Keywords

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