• Asian Pacific Journal of Cancer Prevention
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• 제13권2호
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• pp.653-664
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• 2012

Objective: This study assessed anti-inflammatory and antioxidant activities of $E.$ $foetidum$ leaf extract on LPS-activated murine macrophages. Methods: RAW264.7 cells were pretreated with or without $E.$ $foetidum$ extract for 1 h prior to incubation with LPS for 24 h. Anti-inflammatory activity was evaluated with reference to iNOS, COX-2, TNF-${\alpha}$ and IL-6 gene expression. In addition, NO and intracellular ROS generation were determined by Griess method and fluorescence intensity and activation of MAPKs and $I{\kappa}B$ by Western blotting. Results: Prior treatment with $E.$ $foetidum$ leaf extract inhibited elevation of IL-6, TNF-${\alpha}$, iNOS and COX-2, together with their cognate mRNAs in a dose-dependent manner. NO and intracellular ROS contents were similarly reduced. These effects were due to inhibition of LPS-induced phosphorylation of JNK and p38 as well as $I{\kappa}B$. $E.$ $foetidum$ ethanol extract were shown to contain lutein, ${\beta}$-carotene, chlorogenic acid, kaempferol and caffeic acid, compounds known to exert these bioactive properties. Conclusions: $E.$ $foetidum$ leaf extract possesses suppressive effects against pro-inflammatory mediators. Thus, $E.$ $foetidum$ has a high potential to be used as a food supplement to reduce risk of cancer associated with inflammation.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2009년도 추계학술발표논문집
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• pp.208-211
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• 2009

본 연구에서는 해부학 실습에 사용되었던 사체와 인체 모형을 활용하여 해부설명회를 개최하였고, 의학전문대학원생들이 간호학과, 응급구조학과, 스포츠 과학부 등 3개 학과 학부생들을 대상으로 사체 내부의 구조와 기능을 설명하고, 상호 질의응답과 토론을 진행한 후, 설문조사와 소감문을 통하여 학습 효과를 분석하였다. 해부학 설명자 요인, 설명 및 설명회 전체 만족도, 학습도움정도에 있어서 참가자가 설명자보다 유의하게 높은 수치를 보였다. 기반시설이나 교육여건이 아니라 설명 자체의 만족도가 설명회 전체 만족도를 결정하였으며, 학습에 도움을 주는 가장 중요한 요인은 해부학 설명자 요인으로 태도, 방법, 설명 내용 등이 포함되었다. 참가자들은 낯선 수업방식에도 불구하고 이론수업을 통해 얻은 해부학 지식을 체계화하는데 도움을 받았고, 설명자는 참가자를 위해 적절한 용어, 설명속도, 질의응답 등 의사소통의 중요성과 필요성을 깨닫게 되었다. 결론적으로 건강-보건-의료 분야의 학생들을 대상으로 해부설명회를 개최하여 해부학 실습에 대한 학생들의 능동적인 참여를 유도하였고, 의사소통을 통한 정보 전달과정에서 학습내용을 체계화하였을 뿐만 아니라 학문 간의 연계성, 타 전공자와의 상호교류를 통한 학문적 이해의 폭이 확대되었음을 확인하였다. 최근 건강-의료 분야의 학과 신설 및 전공자가 급격하게 늘어남에 따라 해부학 교육의 질적 향성 및 학습자의 학습효과 증진을 도모하기 위해서는 새로운 강의방식의 도입이 필요하다. 따라서 본 연구는 해부학 과목이 전공필수로 포함되어 있는 강원대학교 2개 학과(간호학과, 스포츠과학부) 전공자들을 대상으로 해부학 강의를 위하여 강원대학교 e-러닝 센터와 함께 가상강의 컨텐츠를 개발 과정에 있어 담당교수의 역할을 분석하고, 정규교육과정에서 적용한 후, 학생들의 설문 조사와 가상강의실 운영 성과를 평가하였다.

• Applied Biological Chemistry
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• 제37권6호
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• pp.441-446
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• 1994

된장의 새로운 기능성을 검색하기 위해 된장의 ACE 활성 저해효과를 측정하였다. 7개의 시중제품중 ACE 활성 저해효과가 가장 높은 된장의 열수추출물을 용매별 분획시 acetone$(50{\sim}80%)$ 분획이 acetonitrile$(50{\sim}80%)$ 분획에 비해 다소 낮은 57% 단백질 회수율을 보였으나, ACE 활성 저해효과는 92.8%로 가장 높은 활성 저해효과를 보였다. 아세톤 분획을 Sephadex G-25, Sephadex LH-20, ODS column chromatography와 HPLC를 이용하여 정제한 결과 수율은 1%, $IC_{50}$ 값은 0.6 mg/ml로 나타났다. 된장으로부터 정제한 저해물질은 경쟁적 저해기작을 나타냈으며, 아미노산 분석결과 Ala, Phe, Leu, Glu, Gly, Ser, Asp 아미노산으로 구성되어 있었다.

• 대한방사선치료학회지
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• 제8권1호
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• pp.55-61
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• 1996

A radiation beam incident on irregular or sloping surface produces an inhomogeneity of absorbed dose. The use of a tissue compensator can partially correct this dose inhomogeneity. The tissue compensator should be made based on experimentally measured thickness ratio. The thickness ratio depends on beam energy, distance from the tissue compensator to the surface of patient, field size, treatment depth, tissue deficit and other factors. In this study, the thickness ratio was measured for various field size of $5cm{\times}5cm,\;10cm{\times}10cm,\;15cm{\times}15cm,\;20cm{\times}20cm$ for 4MV X-ray beams. The distance to the compensator from the X-ray target was fixed, 49cm, and measurement depth was 3, 5, 7, 9 cm. For each measurement depth, the tissue deficit was changed from 0 to(measurement depth-1)cm by 1cm increment. As a result, thickness ratio was decreased according to field size and tissue deficit was increased. Use of a representative thickness ratio for tissue compensator, there was $10\%$ difference of absorbed dose but use of a experimentally measured thickness ratio for tissue compensator, there was $2\%$ difference of absorbed dose. Therefore, it can be concluded that the tissue compensator made by experimentally measured thickness ratio can produce good distribution with acceptable inhomogeneity and such tissue compensator can be effectively applied to clinical radiotherapy.

• Asian Pacific Journal of Cancer Prevention
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• 제13권4호
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• pp.1605-1610
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• 2012

Gelam and Nenas monofloral honeys were investigated in this study for their chemopreventive effects against HT 29 colon cancer cells. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolim) assays showed more effective inhibition of colon cancer cells proliferation by Gelam honey with $IC_{50}$ values of 39.0 mg/ml and 85.5 mg/ml respectively after 24 hours of treatment. Alkali comet assays revealed both honeys increased DNA damage significantly in a dose dependent manner. In addition, annexin V-FITC/PI flow cytometry demonstrated that at $IC_{50}$ concentrations and above, both Gelam and Nenas honeys induced apoptosis significantlyat values higher than for necrosis (p<0.05). Measurement of prostaglandin $E_2$ ($PGE_2$) confirmed that Gelam and Nenas honeys reduced its production in $H_2O_2$ inflammation-induced colon cancer cells. In conclusion, our study indicated and confirmed that both Gelam and Nenas honeys are capable of suppressing the growth of HT 29 colon cancer cells by inducing apoptosis and suppressing inflammation.

• 대한한의학회지
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• 제42권1호
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• pp.99-109
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• 2021

Objectives: Objective: The purpose of this study is to investigate the treatment of herbal medicine for patients with COVID-19 asymptomatic infections. Method: In English, search engines such as 'PubMed', 'Science Direct', and 'Cumulative Index to Nursing and Allied Health Literature (CINAHL)' were used. In Chinese, search engines such as 'China National Knowledge Infrastructure (CNKI)' and Wanfang were used. The keywords for the search engines were 'COVID-19', 'asymptomatic infection', 'Korean Medicine', 'traditional Chinese medicine', 'herbal medicine', and etc. Only clinical studies using herbal medicine for patients without fever or respiratory symptoms were selected. We excluded the cases that do not fit the research topic. Results: A total of 5 studies were finally selected. Among them, four of them used integrated herbal medicine and Western medicine, and one of the studies treated exclusively for herbal medicine. There were a total of seven prescriptions for herbal medicine used in the study. Outcome variables were used as following: lab test, nucleic acid conversion time, hospitalization period, chest CT, and etc. In the RCT study, herbal medicine and Western medicine decreased nucleic acid conversion time, average hospitalization time compared to the control group, but it was not statistically significant. No other adverse reactions were reported in all studies. Conclusion: According to the results, integrated herbal medicine and Western medicine might be an effective treatment for patients with COVID-19 asymptomatic infection reducing hospitalization period, time of nucleic acid turning negative. No severe adverse effects were reported. However, it is thought that better-designed research will be needed in the future.

• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1817-1823
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• 2016

Areca nut (AN) chewing is a habit in many countries in Central, Southern, and Southeast Asia. It is strongly associated with the occurrence of oral, pharyngeal, and esophageal cancer as well as systemic inflammation. However, the association between AN intake and the development of gastric lesions has not yet been identified. The aim of this study was to investigate the effect of AN on gastric diseases using a mouse model for Helicobacter pylori infection. We studied four groups of mice: those fed a normal diet (ND), those fed a diet containing 2.5% AN (AD), those fed ND and infected with H. pylori PMSS1 strain (ND/HP), and those fed AD and infected with H. pylori PMSS1 strain (AD/HP). Food intake and body weight were monitored weekly during the experiments. At 10 weeks, the mice were sacrificed, and the stomach weight, H. pylori colonization, and gastric inflammation were evaluated. The stomach weight had increased significantly in the ND/HP and AD/HP groups along with increases in H. pylori colonization; however, there was no significant difference between these two groups with respect to stomach weight and colonization. On histological grading, mononuclear cell infiltration was severer in the AD/HP group than in the ND/HP group. These data suggest that chronic gastric inflammation was aggravated by AN treatment in the mice with H. pylori-induced gastric lesions. Furthermore, as previously suggested, this animal model is useful to determine the effect of potential carcinogens on gastric lesions induced by H. pylori infection.

• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4339-4345
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• 2015

Occupational exposure to wood dust is one cause of nasopharyngeal cancer (NPC); however, assessing this exposure remains problematic. Therefore, the objective of this study was to develop a semi-quantitative exposure assessment method and then utilize it to evaluate the association between occupational exposure to wood dust and the development of NPC. In addition, variations in risk by histology were examined. A case-control study was conducted with 327 newly diagnosed cases of NPC at the National Cancer Institute and regional cancer centers in Thailand with 1:1 controls matched for age, gender and geographical residence. Occupational information was obtained through personal interviews. The potential probability, frequency and intensity of exposure to wood dust were assessed on a job-by-job basis by experienced experts. Analysis was performed by conditional logistic regression and presented in odds ratio (ORs) estimates and 95% confidence intervals (CI). Overall, a non significant relationship between occupational wood dust exposure and NPC risk for all subjects was observed (ORs=1.61, 95%CI 0.99-2.59); however, the risk became significant when analyses focused on types 2 and 3 of NPC (ORs=1.62, 95%CI 1.03-2.74). The significant association was stronger for those exposed to wood dust for > 10 year (ORs=2.26, 95%CI 1.10-4.63), for those with first-time exposure at age > 25 year (ORs=2.07, 95%CI 1.08-3.94), and for those who had a high cumulative exposure (ORs=2.17, 95%CI 1.03-4.58) when compared with those considered unexposed. In conclusion, wood dust is likely to be associated with an increased risk of type 2 or 3 NPC in the Thai population. The results of this study show that semi-quantitative exposure assessment is suitable for occupational exposure assessment in a case control study and complements the information from self-reporting.

• 대한방사선기술학회지:방사선기술과학
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• 제33권3호
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• pp.203-211
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• 2010

본 연구의 목적은 PBL/S-PBL에 사용되는 패키지(교재)를 측정할 수 있는 타당하고 신뢰할 수 있는 평가도구를 개발하는 것이다. 이러한 목적을 실현하기 위해 문헌 고찰을 통하여 PBL 및 S-PBL 패키지를 측정하는 도구의 3항목 15문항의 예비문항들을 만들었다. 예비문항을 대학의 교수 9인의 전문가를 통해 내용타당도를 검증하여 PBL은 14문항, S-PBL은 15문항으로 구성하였다. 개발된 평가도구의 구성 타당도 검증을 위하여 요인분석을 하였다. PBL은 동기부여 3항목, 통합성 7항목, 체계성 4항목, 총 14항목으로, S-PBL은 동기부여 4항목, 통합성 6항목, 체계성 5항목, 총 15항목으로 이루어진 평가도구가 제시되었으며 Cronbach's $\alpha$계수가 PBL은 0.9112, S-PBL은 0.9166으로 적절한 신뢰도를 갖는 평가도구로 검증되었다.

• 한국산학기술학회논문지
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• 제12권1호
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• pp.281-287
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• 2011

해부학 교육에 있어 가상강의 방식을 활용하는 것은 학문적 특성에 부합되는 교육방식이다. 본 연구는 해부학 교육의 개선 방안의 일환으로서, 가상강의의 도입 및 확대 적용 가능성을 평가하고자 자체개발한 해부학 가상강의 컨텐츠를 바탕으로 2007-2009년 3년 동안 간호학과 및 스포츠과학부 학생들에게 가상강의를 실시하였다. 강의 종료후, 수강학생 중 총 231명을 대상으로 가상강의실 게시판 및 컨텐츠 활용 빈도, 개별 설문조사, 학업성취도 자료를 바탕으로 자기평가요인, 가상강의실 활용도 및 학업성취도의 상관관계를 분석하였다. 그 결과, 학생들은 전공의 구분 없이 모두 만족도, 난이도, 흥미도, 전공연계성 등에서 긍정적인 평가를 하였다. 또한 가상강의실 이용의 편의성 및 과제에 대해서도 높은 만족도를 나타내었으며, 로그인 횟수와 게시판에 대한 학생들의 만족도도 높았다. 학업성취도는 학과간의 유의한 차이를 보였으나, 가상강의실의 로그인 횟수와의 상관관계(r=0.246, p<0.01, 간호학과; r=0.234, p<0.05 스포츠과학부)는 높게 나타났다. 학업성취도와 높은 상관관계를 보이는 자기평가요인은 간호학과의 경우 난이도(r=0.218, p<0.01)였으며, 스포츠과학부는 만족도, 난이도, 흥미도 등(r=0.499, p<0.05)이었다. 따라서 해부학 가상강의는 해부학 강의를 필요로 하는 다양한 전공 분야의 학생들의 교육 수요를 적극적으로 흡수하고, 부족한 해부학 전공 강의요원들의 교육 부담을 경감시킬 것으로 사료된다. 다만, 지속적인 컨텐츠 개발과 운영 효율성에 대한 제고를 통하여 보다 효과적인 교수법으로 거듭날 수 있을 것이다.