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http://dx.doi.org/10.7314/APJCP.2012.13.2.653

Eryngium foetidum Suppresses Inflammatory Mediators Produced by Macrophages  

Mekhora, Chusana (Institute of Nutrition, Mahidol University)
Muangnoi, Channarong (Institute of Nutrition, Mahidol University)
Chingsuwanrote, Pimjai (Institute of Nutrition, Mahidol University)
Dawilai, Suwitcha (Institute of Nutrition, Mahidol University)
Svasti, Saovaros (Institute of Molecular Biosciences, Mahidol University)
Chasri, Kaimuk (Faculty of Allied Health Science, Thammasart University)
Tuntipopipat, Siriporn (Institute of Nutrition, Mahidol University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.2, 2012 , pp. 653-664 More about this Journal
Abstract
Objective: This study assessed anti-inflammatory and antioxidant activities of $E.$ $foetidum$ leaf extract on LPS-activated murine macrophages. Methods: RAW264.7 cells were pretreated with or without $E.$ $foetidum$ extract for 1 h prior to incubation with LPS for 24 h. Anti-inflammatory activity was evaluated with reference to iNOS, COX-2, TNF-${\alpha}$ and IL-6 gene expression. In addition, NO and intracellular ROS generation were determined by Griess method and fluorescence intensity and activation of MAPKs and $I{\kappa}B$ by Western blotting. Results: Prior treatment with $E.$ $foetidum$ leaf extract inhibited elevation of IL-6, TNF-${\alpha}$, iNOS and COX-2, together with their cognate mRNAs in a dose-dependent manner. NO and intracellular ROS contents were similarly reduced. These effects were due to inhibition of LPS-induced phosphorylation of JNK and p38 as well as $I{\kappa}B$. $E.$ $foetidum$ ethanol extract were shown to contain lutein, ${\beta}$-carotene, chlorogenic acid, kaempferol and caffeic acid, compounds known to exert these bioactive properties. Conclusions: $E.$ $foetidum$ leaf extract possesses suppressive effects against pro-inflammatory mediators. Thus, $E.$ $foetidum$ has a high potential to be used as a food supplement to reduce risk of cancer associated with inflammation.
Keywords
E. foetidum; iNOS; COX 2; TNF ${\alpha}$; IL 6; inflammatory mediators;
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