• Korean Journal of Food Science and Technology
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• v.54 no.1
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• pp.8-16
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• 2022

Rice (brown rice: milled rice=50:50) was cooked using different concentrations of hyaluronic acid (HA) solution (0.1, 0.3, 0.5, and 0.7%, respectively) as the cooking water, and the properties of the cooked rice were compared. As the HA content increased, the moisture content of the cooked rice significantly increased, and the textural properties, including hardness, cohesiveness, and adhesiveness, except springiness, significantly decreased. For color, as the HA amount increased, the L^* value decreased, whereas the b^* values increased. The free radical scavenging effect and total polyphenol content also increased significantly as the amount of HA increased. In the sensory test, the hardness of the samples containing HA was higher than that of the control; however, there was no significant difference in the overall acceptability. Based on the above results, much softer cooked brown rice could be produced using HA solution (up to 0.7%) as the cooking water, and additional beneficial characteristics, such as antioxidant effect, can be obtained.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.39 no.4
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• pp.281-288
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• 2013

Hyaluronic acid (HA) is one of the major extracellular matrix components in skin. The HA content is reported to decline with age, which may contribute to decrease in skin moisture, wrinkle formation and the decrease in elasticity of the skin. Among the family of HA synthase genes (HAS-1, 2, 3) identified so far, HAS-2 plays crucial roles in the regulation of HA synthesis in human skin fibroblasts. In this study, we elucidated the effects of ferulic acid isolated from Cnidium officinale on HA production. Semi-quantitative RT-PCR and quantitative real-time PCR showed that ferulic acid increased mRNA level of HAS-2 gene and ELISA assay also revealed that ferulic acid increased HA production in human skin fibroblasts. Our study suggests that ferulic acid might prevent age-dependent skin deteriorations such as wrinkles, dryness and elasticity decrease, all of which could be ascribed to the reduction of the HA content in human skin.

• Polymer(Korea)
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• v.32 no.6
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• pp.561-565
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• 2008

Hyaluronic acid (HA) is a natural glycosaminoglycan and is used widely in the pharmaceutical field. Lipoic acid (LA) helps the regeneration of exogenous and endogenous antioxidants such as Vitamin C and Vitamin E as well as glutathione. It also acts as antioxidant indirectly. Hydrophilic HA as a biodegradable and biocompatible polymer was conjugated with hydrophobic LA as an antioxidant to form the graft copolymer. The carboxyl group of HA was modified by adipic acid dihydrazide (ADH). The synthesis of HA-g-LA graft copolymers was characterized by FT-IR, $^1H$-NMR spectroscopy. The conjugates could form the self-assembled nanoparticles in aqueous solution. The particle size and critical aggregation concentration were verified to use the nanoparticle as a carrier fur the hydrophobic material.

• The Korean Journal of Vision Science
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• v.20 no.4
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• pp.513-521
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• 2018

Purpose : : In this study, we made dopamine-functionalized hydrogels containing a cross-linked hyaluronic acid (HA) network and investigated their antioxidant activities. Methods : In the first step, we made poly hydroxyethyl methacrylate(p(HEMA))-based hydrogels post-modified with an interpenetrating polymer network(IPN) structure composed of HA polymers and a p(HEMA) network. The subsequent functionalization with dopamine via an amide coupling reaction resulted in the antioxidant hydrogels. Their antioxidant activities were evaluated using 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays. Results : The dopamine-modified hydrogels exhibited significant antioxidant activities, when compared to unmodified control. The presence of the HA-IPN structure improved the surface wettability of the hydrogel while dopamine-conjugated IPN hydrogel did not demonstrate the significant difference compared to hydrogel control. Dopamine-modified hydrogels exhibited high transmittance (>88%). Conclusion : The results demonstrate that the development of antioxidant hydrogels based on dopamine-conjugated HA-IPN structures may help develop ophthalmic and biomedical materials.

• Polymer(Korea)
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• v.35 no.2
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• pp.119-123
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• 2011

To develop hyaluronic acid (HA)-based anticancer agent carrier, hyaluronic acid was chemically modified with the hydrophobic group of deoxycholic acid(DA). The physicochemical properties of the deoxycholic acid-conjugated HA (HADA) were investigated by using $^1H$ NMR, FTIR spectrophotometer and TEM. Paclitaxel (Tx)-loaded HADA nanoparticles were prepared by a dialysis method. The loading efficiency of drug and drug contents of Tx-loaded HADA nanoparticles (HADA-Tx) were measured by HPLC. The anticancer activity of HADA-Tx was investigated by its cytotoxicity against KB cell in vitro. The HADA-Tx was shown to have the superior potential for the anticancer drug delivery.

• Journal of Korean Foot and Ankle Society
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• v.15 no.3
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• pp.115-118
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• 2011

Treatment of osteoarthritis of the ankle joint is similar to that of any other large joint and includes conservative and surgical treatments. Surgical option in severe osteoarthritis is joint fusion or joint replacement, whereas conservative treatment is limited and includes mainly ankle supports, physical therapy, and oral medication. Hyaluronic acid was discovered in 1934 and now has been widely used in the knee and shoulder joints. We reviewed the articles about an intra-articular hyaluronic acid injection in the treatment of osteoarthritis of the ankle joint.

• Polymer(Korea)
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• v.35 no.3
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• pp.223-227
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• 2011

Stimuli-responsive polymers have been investigated as the materials playing the critical roles in various applications. Thermoresponsive graft copolymers, poly (N-isopropylacrylamide)-g-hyaluronic acid (PNIPAAm-g-HA) and elastin-like peptide-g-hyaluronic acid (ELP-g-HA), were synthesized by coupling carboxylic polymers (PNIPAAm-COOH or ELP) to biocompatible HA through amide linkages. Thermoresponsive behavior was observed in both the copolymers, and the results of turbidity measurement were consistent with the results of rheological examination. Among the two copolymers, the ELP graft copolymer shows less cooperative LCST transition than the PNIPAAm case. As the content of graft chains of PNIPAAm and ELP increases, viscosity increases, and the increase was larger in PNIPAAm case at a graft content. These results shows us that the introduction of grafts provides thermosensitivity to biocompatible HA, whose characteristics can be engineered.

• Journal of Life Science
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• v.26 no.5
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• pp.545-554
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• 2016

Hyaluronic acid (HA) is an important macromolecule in medical and pharmaceutical fields. HA is a natural and linear polymer composed of repeating disaccharide units of β-1, 3-N-acetyl glucosamine and β-1, 4-glucuronic acid. This work aimed to confirm the structural characteristics and anti-inflammatory activities of HA and its chemically sulfated-HA. HA was produced from a fed-batch fermentation process using Streptococcus dysgalactiae in a 5 l bioreactor. HA was isolated water-soluble form (HA-WS) and water-insoluble form (HA-WI) from culture medium, and was obtained chemically sulfated-derivative (S-HA) that resulted in a 90% yield from HA-WI. The structural features of the sulfated- HA (S-HA) were investigated by FT-IR and ¹H-NMR spectroscopy. The FT-IR and NMR patterns revealed the similarity in both the FTIR spectrum as well as NMR spectrum of both reference standard and purified HA from S. dysgalactiae. The anti-inflammatory activities of HA and S-HA were examined on LPS-induced RAW 264.7 cells. S-HA was significantly inhibited production of pro-inflammatory mediators such as nitric oxide (NO) and PGE₂ and the gene levels of iNOS and COX-2, which are responsible for the production of NO and PGE₂, respectively. Furthermore, S-HA also suppressed the overproduction of pro-inflammatory cytokine TNF-α (<80 pg/ml) and IL-6 (<100 pg/ml) compared to that of HA-WI. The present study clearly demonstrates that HA-S exhibits anti-inflammatory activities in RAW 264.7 macrophage cells.

• Journal of the Korean Society of Radiology
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• v.12 no.1
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• pp.9-16
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• 2018

Hangover after drinking is different from person to person symptoms and degree, but usually thirst, fatigue, headache, general boredom, gastrointestinal disorder, vomiting, diarrhea, deficiency of vitamin appears. This hanging phenomenon is caused by the action of precursors such as ethyl acetate and acetaldehyde, which are the by products of fermentation contained in alcohol and alcohol accumulated in hepatocytes and body. In order to solve the hangover phenomenon, the same Origin as polysaccharide Polydeoxyribonucleotide, which is a nucleic acid-sugar-phosphate complex, which is a semen or testicular extract in salmon extract, and a water soluble salmon extract powder having the same structure and lower price than Polydeoxyribonucleotide And D-Glucuronic acid and N-Acetyl glucosamine. It has excellent biocompatibility, viscoelasticity and moisturizing power. It has effect on reduction of body water loss and skin moisture content in hangover phenomenon. It is antioxidant and skin moisturizing effect Hyaluronic acid was irradiated with gamma rays, and the composition was prepared by using the salmon extract powder and the main raw material. The ethanol degradation, the acetaldehyde reduction amount, the blood acetaldehyde concentration and the acetic acid concentration were measured to evaluate the alcoholysis effect, Skin moisture evaporation rate To examine the evaluation unit water content of the skin was improved determine whether the antioxidant and provide skin moisturizing effect. The addition of ethanol extracts of salmon extracts showed a decrease of 5 to 7 times compared with no addition, and a decrease of 3 to 5 times of acetaldehyde. In addition, the change of acetaldehyde concentration and acetic acid concentration in blood showed a rapid decrease compared to the no - added control group. In addition, when the raw material of hyaluronic acid was used, skin moisture content was high and skin moisture evaporation amount was decreased. Therefore, hyaluronic acid, which is a polysaccharide polymer, has excellent viscoelasticity and moisturizing ability, It is considered to provide antioxidant and skin moisturizing effect. Therefore, it can be said that the composition containing salmon extract powder and hyaluronic acid as a main ingredient is effective for the hangover phenomenon which occurs after drinking.

• Journal of the Korean Applied Science and Technology
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• v.35 no.4
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• pp.1487-1495
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• 2018

In this study, we investigated collagen production and hyaluronic acid production effects for wrinkle improvement test on 50 kinds of land plants and 10 kinds of marine plants native to Jeju Island as a part of developing customized cosmetic materials. Collagen and hyaluronic acid are recognized as major factors affecting skin aging. Cerastium holosteoides var. hallaisanense Mizushima extract ($100{\mu}g/mL$) produced more than 190% of collagen in the extracts of 50 kinds of land plants. Vicia angustifolia var. segetilis K. Koch. extract ($100{\mu}g/mL$) produced more than 160% of collagen. Ftsia japonica Decne. et Planch. extract ($100{\mu}g/mL$), Euonymus japonica Thunb. extract ($100{\mu}g/mL$), Suaeda malacosperma H.Hara extract ($100{\mu}g/mL$), Elaeagnus umbelellata Thunb. extract ($100{\mu}g/mL$), Sedum oryzifolium Makino extract ($100{\mu}g/mL$), Vicia unijuga A. Br. extract ($100{\mu}g/mL$), and Brassica juncea var. integrifolia Sinsk. extract ($100{\mu}g/mL$) showed more than 140% collagen production effect. Among the 10 species of marine plants, Sargassum macrocarpum C. Agardh extract ($50{\mu}g/mL$) produced more than 190% of collagen, and Carpopeltis angusta (Harvey) Okamura extract ($100{\mu}g/mL$), Codiumcoactum Okamura extract ($100{\mu}g/mL$), and Codium tenuifolium S. Shimada, T. Tadano & J. Tanaka extract ($100{\mu}g/mL$) showed more than 140% collagen production. Suaeda malacosperma H.Hara extract ($100{\mu}g/mL$) showed the effect of producing hyaluronic acid more than 140%, and Ftsia japonica Decne. et Planch. extract ($20{\mu}g/mL$) and Wistaria floribunda A.P. DC extract ($100{\mu}g/mL$) showed more than 130% hyalunonic acid production effect. Among the 10 species of marine plants, Peyssonnelia capensis Montagne extract ($100{\mu}g/mL$) was the most effective. Carpopeltis angusta (Harvey) Okamura extract ($100{\mu}g/mL$), Codiumcoactum Okamura extract ($100{\mu}g/mL$), and Codium tenuifolium S. Shimada, T. Tadano & J. Tanaka extract ($100{\mu}g/mL$) showed more than 120% hyalunonic acid production. Jeju resources, which have good collagen and hyaluronic acid production, showed the potential to be applied to solve the skin troubles of customized cosmetics in the future.