Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
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Preparation and Characterization of Deoxycholic Acid-Grafted Hyaluronic Acid as a Durg Carrier

약물전달체로서 디옥시콜산이 결합된 히알루론산의 제조와 특성

  • Choi, Chang-Yong (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Park, Jun-Kyu (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Kim, Won-Suk (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Jang, Mi-Kyeong (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Nah, Jae-Woon (Department of Polymer Science and Engineering, Sunchon National University)
  • 최창용 (순천대학교 공과대학 고분자공학과) ;
  • 박준규 (순천대학교 공과대학 고분자공학과) ;
  • 김원석 (순천대학교 공과대학 고분자공학과) ;
  • 장미경 (순천대학교 공과대학 고분자공학과) ;
  • 나재운 (순천대학교 공과대학 고분자공학과)
  • Received : 2010.08.17
  • Accepted : 2010.11.16
  • Published : 2011.03.25
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Abstract

To develop hyaluronic acid (HA)-based anticancer agent carrier, hyaluronic acid was chemically modified with the hydrophobic group of deoxycholic acid(DA). The physicochemical properties of the deoxycholic acid-conjugated HA (HADA) were investigated by using $^1H$ NMR, FTIR spectrophotometer and TEM. Paclitaxel (Tx)-loaded HADA nanoparticles were prepared by a dialysis method. The loading efficiency of drug and drug contents of Tx-loaded HADA nanoparticles (HADA-Tx) were measured by HPLC. The anticancer activity of HADA-Tx was investigated by its cytotoxicity against KB cell in vitro. The HADA-Tx was shown to have the superior potential for the anticancer drug delivery.

본 연구에서는 항암제 전달체로 응용하기 위하여 천연고분자인 히알루론산(hyaluronic acid, HA)에 소수 성기 도입을 위하여 담즙산(bile acid) 중 하나인 디옥시콜산(deoxycholic acid)(DA)을 개질하여 양친성 공중합체를 제조하였고, 이를 항암제 전달체로 응용하고자 하였다. 디옥시콜산이 결합된 히알루론산(HADA)의 물리화학적 특성은 $^1H$ NMR, FTIR, spectrophotometer와 TEM을 이용하여 측정하였다. 디옥시콜산이 결합된 히알루론산에 항암제(파클리탁셀)를 투석방법을 통하여 봉입시켰고, in vitro에서 KB 세포에 대한 항암활성을 확인하였다. 제조된 디옥시콜산이 결합된 히알루론산이 항암제 전달체로서의 응용 가능성을 제시하였다.

Keywords

References

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