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http://dx.doi.org/10.5352/JLS.2016.26.5.545

Structural Characteristics and Anti-inflammatory Activities of Chemically Sulfated-hyaluronic Acid from Streptococcus dysgalactiae  

Hong, Chang-Il (Department of Life Science and Biotechnology, Soonchunhyang University)
Jung, Eui-Gil (Department of Life Science and Biotechnology, Soonchunhyang University)
Han, Kook-Il (Department of Life Science and Biotechnology, Soonchunhyang University)
Kim, Yong Hyun (BMI Korea Co. Ltd.)
Lee, Sung Hee (BMI Korea Co. Ltd.)
Lee, Hong Sub (Laboratory of Microbiology, Il-dong Pharmaceutical Co. Ltd.)
Han, Man-Deuk (Department of Life Science and Biotechnology, Soonchunhyang University)
Publication Information
Journal of Life Science / v.26, no.5, 2016 , pp. 545-554 More about this Journal
Abstract
Hyaluronic acid (HA) is an important macromolecule in medical and pharmaceutical fields. HA is a natural and linear polymer composed of repeating disaccharide units of β-1, 3-N-acetyl glucosamine and β-1, 4-glucuronic acid. This work aimed to confirm the structural characteristics and anti-inflammatory activities of HA and its chemically sulfated-HA. HA was produced from a fed-batch fermentation process using Streptococcus dysgalactiae in a 5 l bioreactor. HA was isolated water-soluble form (HA-WS) and water-insoluble form (HA-WI) from culture medium, and was obtained chemically sulfated-derivative (S-HA) that resulted in a 90% yield from HA-WI. The structural features of the sulfated- HA (S-HA) were investigated by FT-IR and 1H-NMR spectroscopy. The FT-IR and NMR patterns revealed the similarity in both the FTIR spectrum as well as NMR spectrum of both reference standard and purified HA from S. dysgalactiae. The anti-inflammatory activities of HA and S-HA were examined on LPS-induced RAW 264.7 cells. S-HA was significantly inhibited production of pro-inflammatory mediators such as nitric oxide (NO) and PGE2 and the gene levels of iNOS and COX-2, which are responsible for the production of NO and PGE2, respectively. Furthermore, S-HA also suppressed the overproduction of pro-inflammatory cytokine TNF-α (<80 pg/ml) and IL-6 (<100 pg/ml) compared to that of HA-WI. The present study clearly demonstrates that HA-S exhibits anti-inflammatory activities in RAW 264.7 macrophage cells.
Keywords
Anti-inflammatory activity; hyaluronic acid; proinflammatory mediators; Streptococcus dysgalactiae; sulfated-hyaluronic acid;
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