• Journal of the Korean Society for Precision Engineering
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• v.17 no.11
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• pp.25-35
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• 2000

• Analytical Science and Technology
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• v.34 no.6
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• pp.275-283
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• 2021

This study presents the development of cell-based microfluidic chips for the performance of acute eye irritation tests due to chemicals and examined some of their applications. Microfluidic chips were fabricated by photolithography and soft lithography, and they had three compartments with different areas for cell culture. Rabbit corneal epithelial cells were used for the eye irritation test. The death of cells cultured inside the chip was monitored at regular time intervals after treatment with an aqueous solution of chemicals, and the cell death rate constants were calculated based on the viability curve. The performance of the microfluidic chip was verified by examining the effects of cell-cell junctions, cell-substrate adhesion, and initial cell numbers compared to cell death rates. Eye irritation tests were performed at various concentrations of an aqueous solution of sodium dodecyl sulfate (SDS), a standard substance for the eye irritant test. The cells were exposed to the SDS aqueous solution for 300 s, and the resulting eye irritation was assessed by cell viability. Finally, the equation for calculating the toxicity score (TS) was derived based on the weighting factor for each compartment in the chip. The cell-based microfluidic chip developed in this study may be used for eye irritation tests from chemicals used in cosmetics and pharmaceuticals.

• Journal of the Microelectronics and Packaging Society
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• v.24 no.2
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• pp.11-15
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• 2017

As the heat dissipation problem is increased in 3D multi flip chip packages, an improvement of thermal conductivity in bonding interfaces is required. In this study, the effect of alumina filler addition was investigated in non-conductive paste(NCP). The fine alumina filler having average particles size of 400 nm for the fine pitch interconnection was used. As the alumina filler content was increased from 0 to 60 wt%, the thermal conductivity of the cured product was increased up to 0.654 W/mK at 60 wt%. It was higher value than 0.501 W/mK which was reported for the same amount of silica. It was also found out that the addition of fine sized alumina filler resulted in the smaller decrease in thermal conductivity than the larger sized particles. The viscosity of NCP with alumina addition was increased sharply at the level of 40 wt%. It was due to the increase of the interaction between the filler particles according to the finer particle size. In order to achieve the appropriate viscosity and excellent thermal conductivity with fine alumina fillers, the highly efficient dispersion process was considered to be important.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.36 no.9
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• pp.901-905
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• 2012

We present an electroporation and viability monitoring chip for lung cancer cells in a single channel with multiple electric field zones. Previous electroporation chips utilized multiple microchannels or electrodes to form multiple electric fields, thus resulting in complex structures. However, the present chip can generate multiple electric fields in a single stepwise microchannel between a pair of electrodes, thus achieving the analysis of both cell electroporation and viability with a simple structure. We demonstrate that the electric field of 0.4 kV/cm results in a maximum percentage of $51.4{\pm}3.0%$ and $26.6{\pm}0.7%$ of viable and electroporated human lung cancer cells, H23 and A549, respectively. The present chip has potential for use in integrated cell chips for transfection studies.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2015.05a
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• pp.134-135
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• 2015

실리콘 반도체 칩 가공기술의 미세화는 40년에 걸쳐 전자기기 진보에 큰 공헌을 할 수 있었다. 절반간격(Half Pitch)이라는 최소 패턴크기로 좁아지고 있다. 회로패턴을 평면적으로뿐만 아니라 집적도를 올리는 3차원 실장기술이 중요시 되었다. 종래칩 표면에만 존재했던 접속용 전극을 표면과 뒷면에 붙여 칩을 관통하는 미세실리콘 관통전극(TSV; Through Silicon Via)제조기술로써 TSV는 한계의 반도체기술을 극복하여 한층 더 크게 발전할 가능성을 비추고 있다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.931-934
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• 2009

본 논문은 $24{\mu}m$ 이하의 미세 폭 및 $30{\mu}m$ 이하의 피치와 같이 미세 패턴을 가진 칩 온 필름(chip-on-film, COF)에 발생한 결함들을 자동으로 검출할 수 있는 시스템을 제안한다. 개발된 검출시스템은 미세 패턴의 COF에서 발생한 개방 (open), 단락 (hard short), mouse bite 및 near short (soft short)과 같은 다양한 결함들을 자동으로 빠르게 검출할 수 있는 기술이 적용되어 있다. 본 논문에서 제안하는 결함 검사 기술의 기본 원리는 미세 패턴내의 결함으로 인해 발생한 저항의 미세 변화를 고주파 공진기 (resonator)를 이용하여 측정 주파수에서 증폭시키고 증폭된 결함 신호와 결함이 없는 경우의 신호와의 전압차를 읽어서 0이 아니면 결함이 있음을 판단한다. 제안된 시스템은 미세 패턴 COF 검사 과정에서 결함들을 신속히 측정할 수 있으므로 불필요한 COF 복사를 위해 소요되는 경비를 줄일 수 있으리라 기대한다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2010.10a
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• pp.718-721
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• 2010

본 논문은 고주파 시스템-온-칩 응용을 위한 자동 보상 시스템을 제안한다. 이러한 시스템은 고주파 회로 칩 제작과정에서 예기치 않게 발생한 미세한 PVT(공정, 전압, 온도) 변동으로 인한 회로 성능 변수들의 미세변동을 검출하여 이를 자동으로 보상한다. 자동으로 보상 가능한 고주파 회로 성능 변수들은 중요한 요소인 입력 임피던스, 전압이득 및 잡음지수를 포함한다. 이러한 시스템은 미세 변동을 자동으로 보상할 수 있도록 고주파 신호를 직류 신호로 변환하는 DFT(Design-for-Testability) 회로를 포함한다.

• KSBB Journal
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• v.22 no.6
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• pp.387-392
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• 2007

Recently, microfluidic biochips for DNA microarray are providing a number of advantages such as, reduction in reagent volume, high-throughput parallel sample screening, automation of processing, and reduction in hybridization time. Particularly, the enhancement of target probe hybridization by decrease of hybridization time is an important aspect highlighting the advantage of microfluidic DNA microarray platform. Fundamental issues to overcome extremely slow diffusion-limited hybridization are based on physical, electrical or fluidic dynamical mixing technology. So far, there have been some reports on the enhancement of the hybridization with the microfluidic platforms. In this review, their principle, performance, and outreaching of the technology are overviewed and discussed for the implementation into many bio-applications.

• Korean Chemical Engineering Research
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• v.53 no.4
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• pp.472-477
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• 2015

This study presents modified microfluidic picoinjector combined Faraday moat with silver nanoparticle electrode to increase electrical efficiency and fabrication yield. We perform simple dropping procedure of silver nanoparticles near the picoinjection channel, which solve complicate fabrication process of electrode deposition onto the microfluidic picoinjector. Based on this approach, the microfluidic picoinjector can be reliably operated at 180 V while conventional Faraday moat usually have performed above 260 V. Thus, we can reduce the operation voltage and increase safety. Furthermore, the microfluidic picoinjector is able to precisely control injection volume from 7.5 pL to 27.5 pL. We believe that the microfluidic picoinjector will be useful platform for microchemical reaction, biological assay, drug screening, cell culture device, and toxicology.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.20 no.3
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• pp.122-128
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• 2021

Machining operations require removal of chips to keep the coolant clean and fresh throughout the operation time. In this study, microchip removal equipment was developed using AutoCAD and CATIA programs for 3D modeling and 2D draft. In addition, the flow analysis and electromagnetic field analysis of the equipment were performed using the COMSOL Multiphysics program. The flow design of the coolant oil tank was realized on the basis of fluid analysis results. Further, on the basis of magnetic density analysis, a conveyer was designed for effectively removing metal microchips in the tank by using arrays of neodymium permanent magnets.