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http://dx.doi.org/10.5806/AST.2021.34.6.275

Fabrication and application of cell-based microfluidic chip for eye-irritation test of chemicals  

Cho, Sujin (Department of Chemistry, Kongju National University)
Rhee, Seog Woo (Department of Chemistry, Kongju National University)
Publication Information
Analytical Science and Technology / v.34, no.6, 2021 , pp. 275-283 More about this Journal
Abstract
This study presents the development of cell-based microfluidic chips for the performance of acute eye irritation tests due to chemicals and examined some of their applications. Microfluidic chips were fabricated by photolithography and soft lithography, and they had three compartments with different areas for cell culture. Rabbit corneal epithelial cells were used for the eye irritation test. The death of cells cultured inside the chip was monitored at regular time intervals after treatment with an aqueous solution of chemicals, and the cell death rate constants were calculated based on the viability curve. The performance of the microfluidic chip was verified by examining the effects of cell-cell junctions, cell-substrate adhesion, and initial cell numbers compared to cell death rates. Eye irritation tests were performed at various concentrations of an aqueous solution of sodium dodecyl sulfate (SDS), a standard substance for the eye irritant test. The cells were exposed to the SDS aqueous solution for 300 s, and the resulting eye irritation was assessed by cell viability. Finally, the equation for calculating the toxicity score (TS) was derived based on the weighting factor for each compartment in the chip. The cell-based microfluidic chip developed in this study may be used for eye irritation tests from chemicals used in cosmetics and pharmaceuticals.
Keywords
cell-based microfluidic chip; eye irritation test; adherent cells; cell death rate; toxicity scores;
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1 K.-M. Jung, S.-H. Lee, Y.-H. Ryu, W.-H. Jang, H.-S. Jung, J.-H. Han, S.-H. Seok, J.-H. Park, Y. Son, Y.-H. Park and K.-M. Lim, Toxicol. In Vitro, 25(1), 403-410 (2011).   DOI
2 M. P. Vinardell and M. Mitjans, J. Pharm. Sci., 97(1), 46-59 (2008).   DOI
3 M. Lee, J.-H. Hwang and K.-M. Lim, Toxicol. Res., 33(3), 191-203 (2017).   DOI
4 M. K. Prinsen, C. F. M. Hendriksen, C. A. M. Krul and R. A. Woutersen, Regul. Toxicol. Pharmacol., 85, 132-149 (2017).   DOI
5 A. Schrage, S. N. Kolle, M. C. R. Moreno, K. Norman, H. Raabe, R. Curren, B. van Ravenzwaay and R. Landsiedel, Altern. Lab. Anim., 39(1), 37-53 (2011).   DOI
6 S. C. McCormick, F. H. Kriel, A. Ivask, Z. Tong, E. Lombi, N. H. Voelcker and C. Priest, Micromachines, 8(4), 124 (2017).   DOI
7 P. Cui and S. Wang, J. Pharm. Anal., 9(4), 238-247 (2019).   DOI
8 Y. Shi, R. Li, J. Yang and X. Li, Int. J. Physiol. Pathophysiol. Pharmacol., 12(2), 70-78 (2020).
9 S. Reichl, J. Pharm. Pharmacol., 60(3), 299-307 (2008).   DOI
10 D. V. Krysko, T. V. Berghe, E. Parthoens, K. D'Herde and P. Vandenabeele, Methods Enzymol., 442, 307-341 (2008).   DOI
11 G. C. Forcina, M. Conlon, A. Wells, J. Yinuo Cao and S. J. Dixon, Cell Syst., 4(6), 600-610 (2017).   DOI
12 Y. Cong, X. Han, Y. Wang, Z. Chen, Y. Lu, T. Liu, Z. Wu, Y. Jin, Y. Luo and X. Zhang, Micromachines, 11(4), 381 (2020).   DOI
13 S. Syama and P. V. Mohanan, Trends Food Sci. Technol., 110, 711-728 (2021).   DOI
14 M. A. Garcia, W. J. Nelson and N. Chavez, Cold Spring Harb. Perspect. Biol., 10(4), a029181 (2018).   DOI
15 J. Scheel, M. Kleber, J. Kreutz, E. Lehringer, A. Mehling, K. Reisinger and W. Steiling, Regul. Toxicol. Pharmacol., 59, 471-492 (2011).   DOI
16 H. R. Ferdowsian and N. Beck, PLoS ONE, 6(9), e24059 (2011).   DOI
17 식품의약품안전처, 화장품정책과, 화장품법 (법률 제14264호, 2016. 5. 29. 시행), 2016.
18 K. R. Wilhelmus, Surv. Ophthalmol., 45(6), 493-515 (2001).   DOI
19 Y. Takahashi, M. Koike, H. Honda, Y. Ito, H. Sakaguchi, H. Suzuki and N. Nishiyama, Toxicol. In Vitro, 22(3), 760-770 (2008).   DOI
20 S. J. Shukla, R. Huang, C. P. Austin and M. Xia, Drug Discov. Today, 15(23-24), 997-1007 (2010).   DOI
21 R. Parboosing, G. Mzobe, L. Chonco and I. Moodley, Med. Chem., 13(1), 13-21 (2016).   DOI
22 Q. Wu, J. Liu, X. Wang, L. Feng, J. Wu, X. Zhu, W. Wen and X. Gong, BioMed. Eng. OnLine, 19(1), 9 (2020).   DOI
23 T. Tian, S. Cho and S. W. Rhee, BioChip J., 13(2), 142-150 (2019).   DOI
24 S. Orrenius, P. Nicotera and B. Zhivotovsky, Toxicol. Sci., 119(1), 3-19 (2011).   DOI