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http://dx.doi.org/10.3795/KSME-B.2012.36.9.901

Electroporation and Viability Monitoring Chip for Lung Cancer Cells in Single Channel with Multiple Electric Field Zones  

Kim, Min-Ji (Dept. of Bio and Brain Engineering, KAIST)
Kim, Tae-Yoon (Dept. of Bio and Brain Engineering, KAIST)
Cho, Young-Ho (Dept. of Bio and Brain Engineering, KAIST)
Publication Information
Transactions of the Korean Society of Mechanical Engineers B / v.36, no.9, 2012 , pp. 901-905 More about this Journal
Abstract
We present an electroporation and viability monitoring chip for lung cancer cells in a single channel with multiple electric field zones. Previous electroporation chips utilized multiple microchannels or electrodes to form multiple electric fields, thus resulting in complex structures. However, the present chip can generate multiple electric fields in a single stepwise microchannel between a pair of electrodes, thus achieving the analysis of both cell electroporation and viability with a simple structure. We demonstrate that the electric field of 0.4 kV/cm results in a maximum percentage of $51.4{\pm}3.0%$ and $26.6{\pm}0.7%$ of viable and electroporated human lung cancer cells, H23 and A549, respectively. The present chip has potential for use in integrated cell chips for transfection studies.
Keywords
Cell Electroporation; Viability; Multiple Electric Field; Transfection;
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