The purpose of the study is to determine the relationship between the infection control organizational culture, infection prevention environment, and infection control performance of nurses at a children's hospital and to identify factors affecting infection control performance. The subjects of the study were 160 nurses from five children's hospitals, and data collection was conducted from February 19 to 29, 2024. Data analysis was performed using frequency, percentage, mean, standard deviation, and difference analysis of variables using t-test, ANOVA, Pearson's correlation analysis, and multiple regression analysis. As a result of the study, infection control performance was positively correlated with infection control organizational culture (r= .610, p< .001) and infection prevention environment (r= .586, p< .001), and as a result of multiple regression analysis, infection control organization The influencing factors appeared in the following order: culture (β= .369), infection prevention environment (β= .312), medical institution accreditation evaluation experience (β= .165), and infection control education experience (β= .137), and the overall explanatory power was It was 50.8% (F=41.966, p< .001). Based on the results of this study, to carry out infection control in children's hospitals, integrated management including the will and effort of individual nurses, support and policy from medical institutions and the government is needed, and the development of an infection control education program that takes into account the special characteristics of children's hospital nurses. Application is necessary.