The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Effects of Hwanggimacmundong-tang on Body Weight and Gene Expression in Obese Rats with Estrogen Deficiency

황기맥문동탕이 에스트로겐 결핍 Rat의 체중 변화와 비만 관련 유전자 발현에 미치는 영향

  • Lee, Hye-In (Dept. of Korean Medicine Obstetrics & Gynecology, College of Korean Medicine, Dae-Jeon University) ;
  • Yoo, Dong-Youl (Dept. of Korean Medicine Obstetrics & Gynecology, College of Korean Medicine, Dae-Jeon University) ;
  • Yoo, Jeong-Eun (Dept. of Korean Medicine Obstetrics & Gynecology, College of Korean Medicine, Dae-Jeon University)
  • 이혜인 (대전대학교 한의과대학 부인과교실) ;
  • 유동열 (대전대학교 한의과대학 부인과교실) ;
  • 유정은 (대전대학교 한의과대학 부인과교실)
  • Received : 2018.04.27
  • Accepted : 2018.03.16
  • Published : 2018.05.25
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Abstract

Objectives: This study was conducted to assess the effects of Hwanggimacmundong-tang extract (HM) on obese rats with estrogen deficiency. Methods: The experiments were performed with ovariectomized rats as estrogen-deficient obesity model. Rats were grouped NC (Normal Control Group; sham operation group), OC (Obesity Control Group; estrogen-deficient group), HML (20 mg/kg/day), HMH (100 mg/kg/day). HM was administered orally for six weeks. Body weights and serum lipid level were measured, and real-time PCR was performed to estimate the effect of HM on gene expression in liver. Results: HM decreased the total cholesterol and triglyceride in serum. And HM increased leptin, CPT1 gene expression in liver tissue of obese rats with estrogen deficiency. However HM decreased $PPAR{\gamma}$, $PGC-1{\alpha}$, HMGCR, CYP8B1, LPL, ACAT1, ACAT2, ApoB, ACOX gene expression in liver tissue of obese rats with estrogen deficiency. Conclusions: It is concluded that HM reduced total cholesterol and triglyceride in serum, and regulate gene expression related to lipid metabolism. These results indicate Hwanggimacmundong-tang that might have potential for treatment of obesity and complications during the menopause caused by estrogen-deficiency.

Keywords

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