The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Melandrii Herba, Akebia Quinata Decaisne, and Tetrapanax Papyriferus on Milk Secretion and Lactation Related Factors in Postpartum Mice

왕불유행, 목통, 통초가 산후 생쥐의 유즙분비량과 유즙분비 관련 인자에 미치는 영향

  • Lee, Chia-Wei (Dept. of Prescriptionolgy, College of Korean Medicine, Woo-Suk University) ;
  • Lee, Eun-Hee (Dept. of Obstetrics & Gynecology, College of Korean Medicine, Woo-Suk University) ;
  • Lee, Chang-Hyun (Dept. of Anatomy, College of Korean Medicine, Woo-Suk University) ;
  • Kim, Hong-Jun (Dept. of Prescriptionolgy, College of Korean Medicine, Woo-Suk University)
  • 이가위 (우석대학교 한의과대학 방제학교실) ;
  • 이은희 (우석대학교 한의과대학 부인과학교실) ;
  • 이창현 (우석대학교 한의과대학 해부학교실) ;
  • 김홍준 (우석대학교 한의과대학 방제학교실)
  • Received : 2018.04.26
  • Accepted : 2018.03.16
  • Published : 2018.05.25
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: The purpose of this study is to investigate the effect of Melandrii Herba (MH), Akebia Quinata Decaisne (AQ), and Tetrapanax Papyriferus (TP) on milk secretion and aquaporin (AQP) expression in lactating mice. Methods: For the experiment, the mice were divided into three groups, which were orally administered MH (2,720 mg/kg), TP (400 mg/kg) and AQ (2,800 mg/kg) extracts respectively for 3 weeks from Day 1 after the birth, compared with the control group (C group), which was administered distilled water. A group consisted of six infantile mice per postpartum mouse. For comparison with the C group, non-pregnant SKH-1 mice were used as the virgin group. Results: 1. When it comes to the immunohistochemical staining for prolactin receptors in the mammary glands, the AQ and MH groups showed a strong immune response to the secretory epithelial cells constituting the mammary alveoli, while the TP group represented a weaker immune response. 2. In the immunohistochemical staining for AQP in the mammary glands, AQP1 showed a strong immune response in the walls of capillaries and venules around the mammary alveoli, and AQP3 in the epithelial cells constituting the mammary alveoli, and AQP5 in some tissues between the mammary alveoli. AQP1 was expressed in the order of TP group>AQ group=C group>MH group, and AQP3 was MH group and AQ group>TP group=C group, and AQP5 was MH group>C group>AQ group and TP group. 3. In the Western blot, AQP1 was expressed in the order of TP group>AQ group>C group>MH group, and AQP3 was MH group>AQ group>C group>TP roup, and AQP5 was MH group>TP Group>C group>AQ group. All of AQP1, 3, 5 expression were significantly higher in the C group than in the Virgin group. Conclusions: The administration of Akebia Quinata Decaisne, Tetrapanax Papyriferus and Melandrii Herba have the effect of improving prolactin levels in postpartum mice and increasing the expression of prolactin receptor and AQPs in the mammary glands, suggesting that lactation might be enhanced by the development of the mammary glands.

Keywords

References

  1. Gabay MP. Galatogues: Medications that induce lactation. J Hum Lact. 2002;18(3):274-9. https://doi.org/10.1177/089033440201800311
  2. Sim TF, et al. Perspectives and attitudes of breastfeeding women using herbal galactagogues during breastfeeding: a qualitative study. BMC Complementary and Alternative Medicine. 2014;14(1) :216. https://doi.org/10.1186/1472-6882-14-216
  3. Choi EJ, et al. A Survey of Breatfeeding in Korea. Korean Institute for health and social affairs. 2016:17, 33.
  4. Lee SH, Kang HS. The effect of Yongchun- san in the hypogalactia in a mouse. J Korean Obstet Gynecol. 1996;9(1) :191-20.
  5. Lee JK, Kang HS. Effect of Gami-samul -tang on Incomplete Milk Secretion in Postpartum Rats. The Journal of Jeahan Oriental Medical Academy. 1996;1(1):94-105.
  6. Lee EH, Kim TH. Effects of Boheo-tang and Boheo-tang plus Cervi Pantotrichum Cornu : on Lactation in Postpartum mice. J Korean Obstet Gynecol. 2008; 21(4):1-16.
  7. Song YH, Kim TH. Effect on Factors Related Lactation after Administration of Palmul-tang Purpose. J Korean Obstet Gynecol. 2010;23(1):12-29.
  8. Lee AY, et al. Effects of Boheo-tang -gagam on Milk Production and Factors Related Lactation in Postpartum Mice. J Korean Obstet Gynecol. 2016;29(1): 35-52. https://doi.org/10.15204/jkobgy.2016.29.1.035
  9. Endo M, et al. Water channel(Aquaporin1) expression and distribution in mammary carcinomas and glioblastomas. Microvasc Res. 1999;58:89-98. https://doi.org/10.1006/mvre.1999.2158
  10. Matsuzaki T, et al. Expression and immunolocalization of water-channel aquaporins in the rat and mouse mammary gland. Histochem Cell Biol. 2005;123(4-5):501-12. https://doi.org/10.1007/s00418-005-0753-x
  11. Mobasheri A, et al. Cellular localization of aquaporin along the secretory pathway of the lactating bovine mammary gland: an immunohistochemical study. Acta Histochem. 2011;113(2):137-49. https://doi.org/10.1016/j.acthis.2009.09.005
  12. Nazemi S, et al. Reciprocity in the developmental regulation of aquaporins 1, 3 and 5 during pregnancy and lactation in the rat. PLoS ONE. 2014;9(9):e106809. https://doi.org/10.1371/journal.pone.0106809
  13. Liu H, et al. An herbal galactagogue mixture increases milk production and aquaporin protein expression in mammary glands of lactating rats. Evid Based Complement Alternat Med. 2015;2015:760585.
  14. King LS, Agre P. Pathophysiology of the aquaporin water channels. Annu Rev Physiol. 1996;58:619-48. https://doi.org/10.1146/annurev.ph.58.030196.003155
  15. Hua Y, et al. Expression and significance of aquaporins during pregnancy. Frontiers in Bioscience. 2013;18(4):1373-83. https://doi.org/10.2741/4186
  16. Hsu SM, Raine L, Fanger H. The use of antiavidin antibody and avidin-biotin-peroxidase complex in immunoperoxidase technics. Am J Clin Pathol. 1981;75(6):816-21. https://doi.org/10.1093/ajcp/75.6.816
  17. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and applications. Biotechnology. 1992;24:145-9.
  18. Park HJ, et al. Pregnancy outcomes in women aged 35 and older. J Korean Obstet Gynecol. 2006;49(10):2066-74.
  19. Cho JS, Ahn SH. Development and Evaluation of Breastfeeding Promotion Program for Mothers with Breast Engorgement following Cesarean Birth. J Korean Acad Nurs. 2014;44(2):170-8. https://doi.org/10.4040/jkan.2014.44.2.170
  20. The Society of Korean Medicine Obstetrics and Gynecology. 3rd ed. Oriental Obstetrics & Gynecology. Seoul:Euiseongdang. 2016:833-7.
  21. Song IS. Effect of Tongyu-tang and Gamitongyu-tang on the Incomplete Milk Secretion of Postpartum Rats. Department of Oriental Medicine, Graduate School, Kyungsan University. 1995.
  22. Park SW, et al. Effects of Boheo-tang and Saengwha-tang on blood components and hormone content in postpartum rats. J Korean Obstet Gynecol. 2001; 14(2):118-28.
  23. Park JC. A study on the postpartum efficacy. Department of Oriental Medicine, Graduate School, Kyungsan University. 1990.
  24. Agre P, Kozono D. Aquaporin water channels: molecular mechanisms for human diseases. FEBS Lett. 2003; 555:72-8. https://doi.org/10.1016/S0014-5793(03)01083-4
  25. Bwoun D, et al. Localization of the CHIP28 water channel in reabsorptive segments of the rat male reproductive tract. Eur J Cell Biol. 1993;61:264-73.
  26. King LS, Agre P. Pathophysiology of the aquaporin water channels. Annu Rev Physiol. 1996;58:619-48. https://doi.org/10.1146/annurev.ph.58.030196.003155
  27. Mobasheri A, Wray S, and Marples D. Distribution of AQP2 and AQP3 water channels in human tissue microarrays. Journal of Molecular Histology. 2005;36(1-2):1-4. https://doi.org/10.1007/s10735-004-2633-4
  28. Mobasheri A, Barrett-Jolley R. Aquaporin water channels in the mammary gland: from physiology to pathophysiology and neoplasia. Journal of Mammary Gland Biology and Neoplasia. 2014; 19(1):91-102. https://doi.org/10.1007/s10911-013-9312-6
  29. Wu YL. BenCaoCongXin(本草從新). 2nd ed. Seoul:Haenglim Publisher. 1972:57.
  30. Li SZ. BenChaoGangMu(本草綱目). 1st ed. Beijing:People's Medical Publishing House. 1982:1063, 1317, 1319.
  31. Ju YS. Ungok Herbology. 2nd ed. Jeonju:Woosuk press. 2013:731-2, 735-6, 997-8.
  32. Tang SW. ZhongXiuZhengHuoJingShi ZhengLeiBeiYongBenCao(重修政和經 史證類備用本草). Taipei:SMC Publishing Inc. 1976:200.
  33. Riordan J, Gill-Hopple K, Angeron J. Indicators of effective breastfeeding and estimates of breast milk intake. J Hum Lact. 2005;21(4):406-12. https://doi.org/10.1177/0890334405281032
  34. Garton GA. The composition and biosynthesis of milk lipids. Journal of Lipid Research. 1963;4:237-54.
  35. Mannel R, Martens PJ, Walke M. The Core Curriculum for Lactation Consultant Practice. Seoul:The Minjok Medicine News. 2010:123-6, 447-9.
  36. Cursino T, et al. Diuretics vs. placebo for postpartum blood pressure control in preeclampsia (DIUPRE): a randomized clinical trial. Reproductive Health. 2015;12:66. https://doi.org/10.1186/s12978-015-0057-0
  37. Michiko J, Naotoshi S. Effect of Goreisan on the urinary concentrating ability and the expressions of aquaporins in 5/6 nephrectomized rats. Folia Pharmacol. Jpn. 2014;143(2):65-8. https://doi.org/10.1254/fpj.143.65
  38. Kurita T, et al. Effects of Gorei-san: A Traditional Japanese Kampo Medicine, on Aquaporin 1,2,3,4 and V2R mRNA Expression in Rat Kidney and Forebrain. J. Med. Sci. 2011;11(1):30-8. https://doi.org/10.3923/jms.2011.30.38