• Journal of Microbiology and Biotechnology
• /
• v.18 no.6
• /
• pp.1024-1032
• /
• 2008

Efficient expression of the Salmonella Typhimurium tdc ABCDEG operon involved in the degradation of L-serine and L-threonine requires TdcA, the transcriptional activator of the tdc operon. We found that the tdcA gene was transiently activated when the bacterial growth condition was changed from aerobic to anaerobic, but this was not observed if Salmonella was grown anaerobically from the beginning of the culture. Expression kinetics of six tdc genes after anaerobic shock demonstrated by a real-time PCR assay showed that the tdc CDEG genes were not induced in the tdcA mutant but tdcB maintained its inducibility by anaerobic shock even in the absence of tdcA, suggesting that an additional unknown transcriptional regulation may be working for the tdcB expression. We also investigated the effects of nucleoid-associated proteins by primer extension analysis and found that H-NS repressed tdcA under anaerobic shock conditions, and fis mutation delayed the peak expression time of the tdc operon. DNA microarray analysis of genes regulated by TdcA revealed that the genes involved in N-acetylmannosamine, maltose, and propanediol utilization were significantly induced in a tdcA mutant. These findings suggest that Tdc enzymes may playa pivotal role in energy metabolism under a sudden change of oxygen tension.

• Journal of Microbiology and Biotechnology
• /
• v.21 no.3
• /
• pp.252-255
• /
• 2011

Both Escherichia coli (E. coli) and Salmonella enterica serovar Typhimurium (S. Typhimurium) have a tdc operon that encodes enzymes involved in a metabolic pathway for the degradation of L-serine and L-threonine. However, S. Typhimurium does not have the tdcR gene, which is a positive regulator in E. coli. In the present study, transcriptional analysis revealed that tdcA expression in E. coli is higher under anaerobic than aerobic growth conditions, but the opposite is true in S. Typhimurium. Interestingly, a tdcR mutant strain of E. coli showed a similar expression pattern to that observed in S. Typhimurium and was also induced by anaerobic shock. These results suggest that the induction of tdcA expression by anaerobic conditions is observable when tdcA expression is low owing to the absence of TdcR.

• Journal of IKEEE
• /
• v.27 no.3
• /
• pp.234-238
• /
• 2023

In this paper, we propose a time-to-digital converter (TDC) with compensation capability for PVT (process, voltage, and temperature) variations. A typical delay line-based TDC measures time based on the inverter's propagation delay, making it fundamentally sensitive to PVT variations. This paper presents a method to minimize the resolution change of TDC by compensating for the propagation delay caused by the PVT variations. Additionally, it dopts Cyclic Vernier TDC (CVTDC) structure to provide a wide input detection range. The proposed CVTDC with PVT compensation function is designed using a 45nm CMOS process, consumes 8mW of power, offers a TDC resolution of 5 ps, and has an input detection range of about 5.1 ns.

• 전자공학회논문지 IE
• /
• v.49 no.1
• /
• pp.1-6
• /
• 2012

For the performance improvement of a time-to-digital converter(TDC), a 2-stage high resolution TDC has been designed by using a 2-stage vernier time amplifier(2-S VTA). The two stage vernier time amplifier which has a gain over 64 of the resolution can enhance the resolution of the whole two stage TDC. Because of using a vernier TDC, the structure is not limited to advanced processes for achieving high resolution. The proposed TDC has been designed in a $0.18{\mu}m$ CMOS process and simulated with a 1.8V supply voltage. The entire input range is 512ps, and the full resolution 0.125ps.

• Nuclear Engineering and Technology
• /
• v.55 no.2
• /
• pp.484-492
• /
• 2023

Time-to-digital converters (TDCs) based on the tapped delay line (TDL) architecture have been widely used in various applications requiring a precise time measurement. However, the poor uniformity of the propagation delays in the TDL implemented on FPGA leads to bubble error and large nonlinearity of the TDC. The purpose of this study was to develop an advanced TDC architecture capable of minimizing the bubble errors and improving the linearity. To remove the bubble errors, the decimated delay line (DDL) architecture was implemented on the UltraScale + FPGA; meanwhile, to improve the linearity of the TDC, a histogram uniformization (HU) and multi-chain TDL (MCT) methods were developed and implemented on the FPGA. The integral nonlinearities (INLs) and differential nonlinearities (DNLs) of the plain TDCs with the 'HU method' (HU TDC) and with 'both HU and MCT methods' (HU-MCT TDC) were measured and compared to those of the TDC with 'DDL alone' (plain TDC). The linearity of HU-MCT TDC were superior to those of the plain TDC and HU TDC. The experiment results indicated that HU-MCT TDC developed in this study was useful for improving the linearity of the TDC, which allowed for high timing resolution to be achieved.

• Journal of the Institute of Electronics and Information Engineers
• /
• v.52 no.2
• /
• pp.195-200
• /
• 2015

This paper presents SVBS-TDC (Semi-Vernier Binary-Search Time-to-Digital Converter) for the noise improvement of ADPLL (All-Digital Phase Locked Loop. We used a Semi-Vernier BS-TDC (Binary-Search TDC) architecture to improve the operation speed more then 10 times compared with the previous conventional BS-TDC and ensured a 510ps wide input range. The proposed Semi-Vernier BS-TDC was designed in a 65ns CMOS process and the simulation results showed 200MHz speed and 4ps resolution with a 1.2V supply voltage, and considerable noise improvement of ADPLL.

• Journal of the Korean Society of Industry Convergence
• /
• v.19 no.3
• /
• pp.109-124
• /
• 2016

The objective of this paper is to find the density, stiffness, and strength of truss-wall diamond corrugation model combined with pinwheel truss inside space. The truss-wall diamond corrugation (TDC) model is defined as a unit cell coming from solid-wall diamond corrugation (SDC) model. Pinwheel truss-wall diamond corrugation (P-TDC) model is made by TDC connected with pinwheel structure inside of the space. Derived ideal solutions of P-TDC is based on truss-wall and pinwheel truss model at first. And then it is compared with Gibson-Ashby's ideal solution. To validate the ideal solutions of the P-TDC, ABAQUS software is used to predict the density, strength, and stiffness, and then each of them are compared to the ideal solution of Gibson-Ashby with a log-log scale. Applied material property is stainless steel 304 because of having cost effectiveness. Applied parameters for P-TDC are 1 thru 5 mm diameter within fixed opening width as 4mm. In conclusion, the relative Young's modulus and relative yield strength of the P-TDC unit model is reasonable matched to the ideal expectations of the Gibson-Ashby's theory. In nearby future, P-TDC model is hoped to be applied to make sandwich core structure by advanced technologies such as 3D printing skills.

• Journal of the Korean Society for Precision Engineering
• /
• v.14 no.6
• /
• pp.106-113
• /
• 1997

A new algorithm based upon TDC (Time Delay Control) is proposed to improve the robustness of TDC performance in systems where the stick-slip friction is strong. Experiments were performed at the different levels of friction. The reponses of the TDC and the modified TDC were compared each other, and against those of a PID controller with an anti-windup. The results show that the TDC and the modified TDC equally perform better than the PID, and that the modified TDC performs consistently well even with variations in the friction level while the TDC does not.

• Molecules and Cells
• /
• v.28 no.4
• /
• pp.389-395
• /
• 2009

We previously observed that the transcription of some flagellar genes decreased in Salmonella Typhimurium tdcA mutant, which is a gene encoding the transcriptional activator of the tdc operon. Since flagella-mediated bacterial motility accelerates the invasion of Salmonella, we have examined the effect of tdcA mutation on the invasive ability as well as the flagellar biosynthesis in S. Typhimurium. A tdcA mutation caused defects in motility and formation of flagellin protein, FliC in S. Typhimurium. Invasion assays in the presence of a centrifugal force confirmed that the defect of flagellum synthesis decreases the ability of Salmonella to invade into cultured epithelial cells. In addition, we also found that the expression of Salmonella pathogenicity island 1 (SPI1) genes required for Salmonella invasion was down-regulated in the tdcA mutant because of the decreased expression of fliZ, a positive regulator of SPI1 transcriptional activator, hilA. Finally, the virulence of a S. Typhimurium tdcA mutant was attenuated compared to a wild type when administered orally. This study implies the role of tdcA in the invasion process of S. Typhimurium.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.12 no.4
• /
• pp.411-417
• /
• 2012

A Coarse-Fine Time-to-Digital Converter (TDC) using the single time amplifier is proposed. A vernier delay line is used to overcome process dependency and the 2-stage time amplifier is designed to have high resolution by increasing the gain of the time amplifier. Single time amplifier architecture reduces the silicon area of the TDC and alleviates mismatch effect between time amplifiers. The proposed TDC is implemented in $0.18{\mu}m$ CMOS process with the supply voltage of 1.8 V. The measured results show that the resolution of the TDC is 0.73 ps with 10-bit digital output, although highend process is not applied. The single time amplifier architecture reduces 13% of chip area compared to previous work. By reducing the supply voltage, the linearity of the TDC is enhanced and the resolution is decreased to 1.45 ps.