• Journal of Mushroom
• /
• v.11 no.4
• /
• pp.208-213
• /
• 2013

The contents of raw materials which are components of mixed substrate for mushroom cultivation were analyzed to optimize the composition. The pure protein(amino acid) level of soybean meal was the highest, 44.02% followed by those of soybean curd residue(31.5%) and cotton seeds meal(30.6%). The non protein nitrogen(NPN) contents in crude protein of main nitrogen materials were 2.4% for soybean meal and 5.6% for dried soybean curd residue, while those of wheat bran and rice bran used as the carbon source were relatively higher, 17.6% compared to that of nitrogen supplying media. Crude protein content per price was 6.0 for rapeseed meal, indicating that it is high crude protein content per price. Nitrogen-free extract(NFE) considering as an ingredient for mycelial growth were high in alphacorn(72.9%) and wheat bran B(57.2%). Acid detergent fiber(ADF) was high in corncob, 51.88%, its use for cultivation of brown rot fungi including Lentinus lepideus should pay attention because the fungi lack complete lignin degradation activity.

• Korean Journal of Food Science and Technology
• /
• v.27 no.3
• /
• pp.394-403
• /
• 1995

Various thermal treatments such as toasting, roasting, drum drying, autoclaving+drum drying, microwaving+drum drying and extrusion were attempted to investigate their effects on chemical composition (proximate composition, Klason lignin, neutral sugar, uronic acid, starch, free sugar, dietary fiber, phytic acid, etc) and functional properties (water solubility index, water absorption index, water holding capacity, oil holding capacity, bulk density, swelling, etc) of wheat bran. Thermal treatments on wheat bran increased soluble dietary fiber (SDF) content from 2.7% (raw sample) to 4.6% (toasted), 4.5% (roasted), 4.6% (drum dried), 5.2% (autoclaved), 3.7% (microwaved), 5.6% (extruded). In contrast, total dietary fiber (TDF) content remained nearly constant regardless of thermal treatments, reflecting the concomitant decrease in insoluble dietary fiber (IDF). It was also found that water holding capacity (WHC) increased $20{\sim}75%$ due to thermal treatments employed in this research. Both bulk density and swelling were not directly relevant to WHC, but thier multiplication exhibited good correlation $(R^2=0.94)$ with WHC. Scanning elctron microscopy (SEM) exhibited that structure of raw wheat bran was significantly modified, whose degree and shape well reflected the types of thermal treatments.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.30 no.1
• /
• pp.43-48
• /
• 2010

This study was conducted to compare the chemical composition, dry matter digestibility (DMD), dry matter intake, and utilization of nitrogen and energy of dairy goats (Saanen), when fed on imported timothy hay (IT), mixture grass hay (MG) and native grass hay (NG), respectively. The experimental trials were conducted from April, 2008 to December, 2008 at the environmental controlled barn of Chungnam National University. Twelve dairy goats (Saanen) were selected which had nearly the same body weight (24.2kg, male). The content of crude protein (CP) of MG hay was higher than that of other diets (p<0.05), but the contents of NDF, ADF, cellulose and lignin of IT and NG diet were higher than those of MG diet (p<0.05). The voluntary DM intake of dairy goats fed with herbage from MG diet (30.7/$BW_{kg}$/day) was higher than that of other diets, but no significant difference was observed between that of IT diet and NG diet (p>0.05). The DMD of MG diet (69.8%) was higher than that of NG diet (62.2%) and IT diet (60.8%) (p<0.05), but no significant difference was observed between that of IT diet and NG diet (p>0.05). In nitrogen utilization, the apparently digested N %, retained % and biological value of dairy goats fed with herbage from MG diet were higher and TG diet was lower (p<0.05). In energy utilization, digestible energy and the energy of apparently digested minus urinary losses of MG diet were higher than those of IT diet and NG diet (p<0.05), but no significant difference was found between IT diet and NG diet (p>0.05). Based on the results, the dry matter intake, DMD and utilization of nitrogen and energy of dairy goats of MG were higher than those of IT diet and NG diet (p<0.05), the DMD and biological value (%) of NG diet was higher than that of IT diet (p<0.05).

• The Korean Journal of Mycology
• /
• v.31 no.2
• /
• pp.59-66
• /
• 2003

Laccase produced by Pycnoporus cinnabarinus SCH-3 isolated from Korea was partially purified using ultrafiltration, anion exchange chromatography and affinity chromatography, The laccase was produced as the predominant extracellular phenoloxidase during primary metabolism. Neither lignin peroxidase nor manganese-dependent peroxidase were detected in the culture fluid. In order to examine the effect of inducers in laccase production, 2,5-xylidine was added in the culture of Pycnoporus cinnabarinus SCH-3. Addition of 2,5-xylidine enhanced 25-fold laccase production. Purified laccase was a single polypeptide having a molecular mass of approximately 66 kDa, as determined by SDS-polyacrylamide gel electrophoresis, and carbohydrate content of 9%. $K_{m}\;and\;V_{max}$ values for laccase with ABTS [2,2-azinobis (3-ethylbenzthiazoline 6-sulfonic acid)] as a substrate (Lineweaver-Burk plot) was determined to be $44.4{\mu}M\;and\;56.0{\mu}mole$, respectively. The optimal pH for laccase activity was found to be 3.0. The enzyme was very stable for 1 hour at $60{\circ}C$. Half-life ($t_{1/2}$) of the enzyme was about 10 min at $80{\circ}C$. Spectroscopic analysis of purified enzyme indicated that the enzyme was typical of copper-containing protein. Substrate specificity and inhibitor studies for laccase also indicated to be a typical fungal laccase. The N-terminal amino acid sequence of the P. cinnabarinus SCH-3 laccase showed 94% of homology to the N-terminal sequences of laccases from P. cinnabarinus PB and P. coccineus.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.11
• /
• pp.1634-1641
• /
• 2005

Generally, dietary fibre (DF) includes lignin, non-starch polysaccharides (NSP) and resistant starch (RS). In monogastric species, low levels of dietary fibre in the diet are associated with various diseases and high levels reduce nutrient digestibilities. In this study, the effects of different types and levels of NSP (soluble: $\beta$-glucan, insoluble cellulose) and resistant starch on mucin secretion and endogenous nitrogen and amino acid losses in pigs were investigated. A total of 25 five-week-old weaner pigs (9.5 kg${\pm}$1.5 kg), were randomly allocated to each of five experimental diets. Different levels of purified barley $\beta$-glucan (BG) extract (5 or 10% of $Glucagel^{(R)}$ $\beta$-glucan, providing 4 or 8% of BG in the diet), and resistant starch (RS) (8.3 or 16.6% of Hi-$Maize^{TM}$, providing 5 or 10% RS in the diet) were substituted for wheat starch in a purified diet in which enzymatically-hydrolysed casein was the sole source of protein. The diets were fed for 21 days. No statistically significant difference between treatments (p>0.05) was observed for growth performance and organs weights. No difference in ileal starch digestibility was observed between pigs on the cellulose or $\beta$-glucan diets. However, as the level of resistant starch in the diet increased the ileal starch digestibility decreased (p<0.05). The inclusion of resistant starch in the diet (5 or 10%) did not increase mucin production when compared with the cellulose-only diet. However, as the level of beta-glucan in the diet increased, both crude mucin in the digesta dry matter and per kg dry matter intake increased (p<0.05). Pigs fed the diet containing 8% of beta-glucan had higher endogenous loss flow than those fed the diets including 5 or 10% of resistant starch or 4% of $\beta$-glucan. In conclusion, dietary inclusion of resistant starch increased the level of starch reaching the large intestine without any effect on mucin secretion, or endogenous nitrogen or amino acid losses content in the small intestine. The addition of $\beta$-glucan to a diet containing cellulose increases both mucin secretion and endogenous amino acid and nitrogen losses in the small intestine.

• Journal of Korean Society of Archives and Records Management
• /
• v.4 no.2
• /
• pp.1-22
• /
• 2004

This study is about the paper of Mulberry(桑紙). The paper which was usually applied to the ancient bookpaper and documents. But there is a rare record about it today. So the paper was made from the Mulberry(뽕나무) bast fiber using traditional handcraft method. and Paper Mulberry(닥나무) by traditional method. And tested by physical and optical methods in comparisons with Paper Mulberry(닥나무). The ratio of length/width of Mulberry fiber was 475, and its lignin content was lower than the Paper Mulberry. The Mulberry paper had similar forming properties and physical strength to the Paper Mulberry fiber. Therefore, the Mulberry fiber seem to be a good paper fiber for traditional paper. For the aging test, in the thermal acceleration treatment for 72 hours and 144 hours at the temperature of $105^{\circ}C$ incubator, the Mulberry paper was more deteriorative than the Paper Mulberry. In the ultraviolet acceleration treatment for 100 hours and 200 hours the Mulberry paper was less interior to the Paper Mullberry, in the increase of treatment time. And the Mulberry paper was approved to be a good traditional paper in appearance. Furthumore, in considing the sample of bred Mulberry species grown today, its paper is thought to be superio to the paper of Paper Mulberry in symptom of senility in natural ultraviolet light.

• Applied Chemistry for Engineering
• /
• v.29 no.3
• /
• pp.350-355
• /
• 2018

In this study, the effect of biomass torrefaction on the thermal and catalytic pyrolysis of cork oak was investigated. The thermal and catalytic pyrolysis behavior of cork oak (CO) and torrefied CO (TCO) were evaluated by comparing their thermogravimetric (TG) analysis results and product distributions of bio-oils obtained from the fast pyrolysis using a fixed bed reactor. TG and differential TG (DTG) curves of CO and TCO revealed that the elimination amount of hemicellulose in CO increased by applying the higher torrefaction temperature and longer torrefaction time. CO torrefaction also decreased the oil yield but increased that of solid char during the pyrolysis because the contents of cellulose and lignin in CO increased due to the elimination of hemicellulose during torrefaction. Selectivities of the levoglucosan and phenolics in TCO pyrolysis oil were higher than those in CO pyrolysis oil. The content of aromatic hydrocarbons in bio-oil increased by applying the catalytic pyrolysis of CO and TCO over HZSM-5 ($SiO_2/Al_2O_3=30$). Compared to CO, TCO showed the higher efficiency on the formation of aromatic hydrocarbons via the catalytic pyrolysis over HZSM-5 and the efficiency was maximized by applying the higher torrefaction and catalytic pyrolysis reaction temperatures of 280 and $600^{\circ}C$, respectively.

• The Korean Journal of Mycology
• /
• v.32 no.2
• /
• pp.112-118
• /
• 2004

Laccase produced by Trametes hirsuta S1 isolated from Korea was partially purified and characterized using ultrafiltration, anion exchange chromatography and affinity chromatography. The laccase was produced as the predominant extracellular enzyme during primary metabolism. Neither lignin peroxidase nor veratryl alcohol oxidase (VAO) were detected in the culture fluid. Addition of 2,5-xylidine enhanced 4-fold laccase production. Purified laccase was a single polypeptide having a molecular mass of approximately 66 kDa, as determined by SDS-polyacrylamide gel electrophoresis, and carbohydrate content of 12%. $K_{m}\;and\;V_{max}$ values for laccase with ABTS [2,2-azinobis (3-ethylbenzthiazoline 6-sulfonic acid)] as a substrate (Lineweaver-Burk plot) was determined to $51.2\;{\mu}M\;and\;56.8\;{\mu}mole$, respectively. The optimal pH for laccase activity was found to be 3.0. The enzyme was very stable for 1 hour at $50^{\circ}C$. Half-life ($t_{1/2}$) of the enzyme was about 20 min at $70^{\circ}C$. Spectroscopic analysis of purified enzyme indicated that the enzyme was typical of copper-containing protein. Substrate specificity and inhibitor studies for laccase also indicated to be a typical fungal laccase. The N-terminal amino acid sequence of the T. hirsuta S1 laccase showed 100% of homology to those of laccase from C. hirsutus.

• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.4
• /
• pp.470-478
• /
• 2017

Objective: This study aims to evaluate the chemical composition, fermentation quality and in vitro ruminal fermentation of various ratios and storage periods of liquid brewer's yeast (LBY) mixed with cassava pulp (CVP). Methods: Four mixtures of fresh LBY and CVP were made (LBY0, LBY10, LBY20, and LBY30 for LBY:CVP at 0:100, 10:90, 20:80, and 30:70, respectively) on a fresh matter basis, in 500 g in plastic bags and stored at 30 to $32^{\circ}C$. After storage, the bags were opened weekly from weeks 0 to 4. Fermentation quality and in vitro gas production (IVGP) were determined, as well as the dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber, acid detergent fiber and acid detergent lignin contents. Results: The contents of CP and EE increased, whereas all other components decreased, in proportion to LBY inclusion (p<0.01). The DM and OM contents gradually decreased in weeks 3 and 4 (p<0.05), while EE contents were lowest in week 0. The pH, ammonia nitrogen per total nitrogen ($NH_3-N/TN$) and V-score in each mixture and storage period demonstrated superior fermentation quality ($pH{\leq}4.2$, $NH_3-N/TN{\leq}12.5%$, and V-score>90%). The pH increased and $NH_3-N/TN$ decreased, with proportionate increases of LBY, whereas the pH decreased and $NH_3-N/TN$ increased, as the storage periods were extended (p<0.01). Although IVGP decreased in proportion to the amount of LBY inclusion (p<0.01), in vitro organic matter digestibility (IVOMD) was unaffected by the mixture ratios. The highest IVGP and IVOMD were observed in week 0 (p<0.01). Conclusion: The inclusion of LBY (as high as 30%) into CVP improves the chemical composition of the mixture, thereby increasing the CP content, while decreasing IVGP, without decreasing fermentation quality and IVOMD. In addition, a preservation period of up to four weeks can guarantee superior fermentation quality in all types of mixtures. Therefore, we recommend limiting the use of CVP as a feed ingredient, given its low nutritional value and improving feed quality with the inclusion of LBY.

• Journal of the Korean Wood Science and Technology
• /
• v.20 no.1
• /
• pp.60-71
• /
• 1992

To investigate the delignification behaviour in solvolysis pulping process, Populus alba ${\times}$ glandulosa H. and Pinus Kuraiensis S. et Z. were cooked with p-cresol and vater solvent(2:8, 5:5, 8:2 v/v) at $175^{\circ}C$ for 9 cooking time levels(20, 40, 60, 80, 100, 120, 140, 160, 180, min). Pulp yield, residual lignin content, de lignification rate, decarborhydration rate were determined. Delignification behaviours were analyzed by TEM. 1. The p-cresol-water solvent cooking of P. alba ${\times}$ glandulosa showed good delignification at the solvent system which the mixture ratio of p-cresol and water were 2:8(v/v), while the cooking of P. koraiensis with the p-cresol and water mixture ratio of 5:5 was no good. 2. P. alba ${\times}$ glandulosa showed three step-delignification phenomena at the solvent system which the mixture ratio of p-cresol and water were 2:8(v/v) anti 5:5(v/v). But P. koraiensis showed a first order delignification reaction at the same mixture ratio of p-cresol and water solvent system. 3. In TEM micrograph obtained for the solvent system which the mixture ratio of p-cresol and water was 5:5(v/v), the partial delignification of the cell corner of P. alba ${\times}$ glandulosa and P. koraiensis were observed at 60min. of cooking time. Complete delignification at the cell corner of P. alba ${\times}$ glandulosa was observed at 160min. and that of P. koraiensis was observed of 180min. of cooking time. 4. In optical microscopic observation, fiber separation of P. alba ${\times}$ glandulosa occured at 120min. and that of P. koraiensis began at 140min. of cooking time. 5. At the solvent system which the mixture ratio of p-cresol and water was 5:5(v/v), middle layer on secondary wall($S_2$) and cell corner of P. alba ${\times}$ glandulosa were more selectively delignified than primary wall(P) and outer layer on secondary wall($S_1$). However P. koraiensis did not showed any difference in delignification between cell wall layers and cell corner.