• Asian-Australasian Journal of Animal Sciences
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• 제21권5호
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• pp.745-753
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• 2008

Gene-manipulated mice were discovered for the first time about a quarter century ago. Since then, numerous sophisticated technologies have been developed and applied to answer key questions about the fundamental roles of the genes of interest. Functional genomics can be characterized into gain-of-function and loss-of-function, which are called transgenic and knock-out studies, respectively. To make transgenic mice, the most widely used technique is the microinjection of transgene-containing vectors into the embryonic pronucleus. However, there are critical drawbacks: namely position effects, integration of unknown copies of a foreign gene, and instability of the foreign DNA within the host genome. To overcome these problems, the ROSA26 locus was used for the knock-in site of a transgene. Usage of this locus is discussed for the gain of function study as well as for several brilliant approaches such as conditional/inducible transgenic system, reproducible/inducible knockdown system, specific cell ablation by Cre-mediated expression of DTA, Cre-ERTM mice as a useful tool for temporal gene regulation, MORE mice as a germ line delete and site specific recombinase system. Techniques to make null mutant mice include complicated steps: vector design and construction, colony selection of embryonic stem (ES) cells, production of chimera mice, confirmation of germ line transmission, and so forth. It is tedious and labor intensive work and difficult to approach. Thus, it is not readily accessible by most researchers. In order to overcome such limitations, technical breakthroughs such as reporter knock-in and gene knock-out system, production of homozygous mutant ES cells from a single targeting vector, and production of mutant mice from tetraploid embryos are developed. With these upcoming progresses, it is important to consider how we could develop these systems further and expand to other animal models such as pigs and monkeys that have more physiological similarities to humans.

• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.417-426
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• 2008

The effect of increasing levels of proclavaminate amidino hydrolase (Pah) on the rate of clavulanic acid production in Streptomyces clavuligerus ATCC 27064 was evaluated by increasing dosoge of a gene (pah2) encoding Pah. A strain (SMF5703) harboring a multicopy plasmid containing the pah2 gene showed significantly retarded cell growth and reduced clavulanic acid production, possibly attributable to the deleterious effects of the multicopy plasmid. In contrast, a strain (SMF5704) carrying a single additional copy of pah2 introduced into chromosome via an integrative plasmid showed enhanced production of clavulanic acid and increased levels of pah2 transcripts. Analysis of transcripts of other genes involved in the clavulanic acid biosynthetic pathway revealed a pattern similar to that seen in the parent. From these results, it appears that clavulanic acid production can be enhanced by duplication of pah2 through integration of a second copy of the gene into chromosome. However, increasing the copy number of only one gene, such as pah2, does not affect the expression of other pathway genes, and so only modest improvements in clavulanic acid production can be expected. Flux controlled by Pah did increase when the copy number of pah2 was doubled, suggesting that under these growth conditions, Pah levels may be a limiting factor regulating the rate of clavulanic acid biosynthesis in S. clavuligerus.

• Asian-Australasian Journal of Animal Sciences
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• 제21권4호
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• pp.499-509
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• 2008

Foxo1 plays an important role in the integration of hormone-activated signaling pathways with the complex transcriptional cascade that promotes preadipocyte differentiation of clonal cell lines from rodents. We isolated the full-length cDNA of porcine FoxO1 gene using RACE, confirmed by visual Northern blotting. The deduced amino acids indicated 94% and 90% identities with the corresponding human and mice aa. Analysis of the aa sequence, showed that it included a Forkhead domain (aa 167-247), a transmembrane structure domain (aa 90-113), a LXXLL motif (aa 469-473), and 51 Ser, 8 Thr, and 4 Tyr phosphorylation sites, indicating a potential important role for FoxO1 transcriptional activity in vivo. Using the IMpRH panel, we mapped FoxO1 gene to chromosome 11p13. Our data provide basic molecular information useful for the further investigation on the function of FoxO1 gene. Time-course analysis of FoxO1 expressions indicated that levels of mRNA and protein gradually increased from day 0 to 3, and it reached almost maximal level at day 3, then decreased from day 5 to 7 in porcine primary preadipocyte differentiation. After induction by IGF-1, GPDH activity and accumulation of lipid increased, however, expressions of FoxO1 mRNA and protein were inhibited in a dose dependent manner. These results suggest that FoxO1 takes part in porcine preadipocyte differentiation and expressions of FoxO1 were regulated by IGF-1.

• 전자공학회논문지
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• 제51권8호
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• pp.172-177
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• 2014

두 가지 $N_2$ 프로세스(성장 중 반응성 질소 그리고 질소 플라즈마 경화)에 의해 특별히 개선된 AsGeTeS 위에 만들어진 문턱 스위칭 소자를 제시하고자 한다. 적층과 열적 안정적인 소자 구조가 가능한 두 스텝 프로세스에서의 질소의 사용은 나노급 배열 회로의 응용에서의 스위치와 메모리 소자의 집적을 가능하게 한다. 이것의 좋은 문턱 스위칭 특성에도 불구하고 AsTeGeSi 기반의 스위치는 높은 온도에서의 신뢰성 있는 저항 메모리 적용에 중요한 요소를 가진다. 이것은 보통 Te의 농도 변화에 기인한다. 그러나 chalconitride 스위치(AsTeGeSiN)은 $30{\times}30(nm^2)$ 셀에서 $1.1{\times}10^7A/cm^2$가 넘는 높은 전류 농도를 갖는 높은 온도 안정성을 보여준다. 스위치의 반복 능력은 $10^8$번을 넘어선다. 더하여 AsTeGeSiN 선택 소자를 가진 TaOx 저항성 메모리를 사용한 1 스위치-1저항으로 구성된 메모리 셀을 시연하였다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2009년도 하계학술대회 논문집
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• pp.52-52
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• 2009

To date, chalcogenide alloy such as $Ge_2Sb_2Te_5$(GST) have not only been rigorously studied for use in Phase Change Random Access Memory(PRAM) applications, but also temperature gap to make different states is not enough to apply to device between amorphous and crystalline state. In this study, we have investigated a new system of phase change media based on the In-Sb-Te(IST) ternary alloys for PRAM. IST chalcogenide thin films were prepared in trench structure (aspect ratio 5:1 of length=500nm, width=100nm) using Tri methyl Indium $(In(CH_3)_4$), $Sb(iPr)_3$ $(Sb(C_3H_7)_3)$ and $Te(iPr)_2(Te(C_3H_7)_2)$ precursors. MOCVD process is very powerful system to deposit in ultra integrated device like 100nm scaled trench structure. And IST materials for PRAM can be grown at low deposition temperature below $200^{\circ}C$ in comparison with GST materials. Although Melting temperature of 1ST materials was $\sim 630^{\circ}C$ like GST, Crystalline temperature of them was ~$290^{\circ}C$; one of GST were $130^{\circ}C$. In-Sb-Te materials will be good candidate materials for PRAM applications. And MOCVD system is powerful for applying ultra scale integration cell.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권1호
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• pp.19-26
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• 2001

Transformed Spodoptera frugiperda (Sf9) cells expressing baculovirus very late factor (VLF-1) were constructed by using Autograha nuclear polyhedrosis virus (AcNPV) immediate earthy gene (ie1). Neomycin-resistance gene as a selectable marker was introduced under the control of AcNPV ie1 promoter, and Bombyx mori nuclear polyhedrosis (BmNPV-K1) vlf-1 gene was introduced under the control of the Drosophila heat shock protein gene (hspr70) promoter to yield dual expression plasmid with two independent transcription units. It was transfected into Sf9 cells and cell clones expressing vlf-1 were selected by G4l8 treatment. Genomic DNA from transformed cells was isolated and integration of AcNPV iel harboring vlf-1 was confirmed by PCR using AcNPV iel-specific primers and Southern blot analysis. The transformed cells expressing VLF-1 in an infection-independent manner expressed foreign gene product of recombinant baculovirus in the earlier stage of infection compared with control Sf9 cells. These results suggest the possible to develop highly efficient transformed insect cells for baculovirus expression vector system.

• 한국수자원학회논문집
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• 제40권4호
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• pp.313-324
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• 2007

비정형격자 기반의 수치해석모형을 이용하여 지하공간침수해석모형을 개발하였다. 본 모형은 지하공간의 흐름 특성을 link-node 시스템에 의해 해석하고, 계단 및 벽구조물 등의 지하공간 구조물 배치 영향을 고려한 침수해석을 수행할 수 있다. 흐름은 두가지로 구분하며, 하나는 두 개의 인접한 격자가 지하공간에서의 지하철 노선에 해당되어 수로형 흐름을 나타내는 경우이고, 다른 하나는 지하공간에서의 지하철 노선 이외의 지점 및 지하상가 등으로 물이 확산되는 위어형 흐름인 경우이다. ArcGIS 시스템의 Visual Basic Application을 이용하여 Dual-Drainage 침수해석 모형과 지하공간침수해석 모형이 통합하였다. 개발된 통합모형은 홍수관계기관이 침수 발생이 예상되는 지점에서 홍수피해 방지를 위해 배수시스템의 용량에 대한 재설계 흑은 확장 등과 같은 대책을 수립하는데 도움을 줄 수 있고, 침수위험지도작성 및 홍수경감대책을 수립하는데 이용될 수 있다.

• 대한의생명과학회지
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• 제10권1호
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• pp.55-63
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• 2004

Bovine leukemia virus (BLV) is a causative agent for lymphoma disease in cattle including cows worldwide. BLV shares similar virion structure and characteristics with other retroviruses. The pol gene of the BLV genome produced reverse transcriptase (RT) and integrase (IN) for important roles for BLV genome integration into host cell chromosomes that is known to be coded in the 3' side of the BLV pol gene (one third portion). In this study, we have sequenced 978 bp in the 3' side of the BLV pol gene from BLV 10C3 in order to determine the BLV IN region of it. And we compared it to the nucleotide sequences of an Australian BLV isolate. As a result, nucleotide sequences of the IN region of the Korean-type BLV pol gene were mutated at a rate of 3.7%. We can confirm that the typical mutations are such as Arg (AGG) $\rightarrow$ Lys (AAG), Thr (ACG) $\rightarrow$ Met (ATG), Ile (ATT) $\rightarrow$ Val (GTT), Asn (ACC) $\rightarrow$ His (CAC), Phe (TTT) $\rightarrow$ Leu (TTG) and Asn (ACC) $\rightarrow$ Asp (GAC). From the analysis of the sequencing data, we were able to determine the zinc-finger-like "HHCC" motif in the amino terminus of BLV IN, that was H-$X_3$-H-$X_{25}-C-X_2$-C. It was also found the DD35E motif in the IN catalytic domain as D-$X_{56}$-D-$X_{35}$-E. It fits very well to the consensus sequences of retroviral IN as well as HHCC motif.

• 대기
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• 제17권4호
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• pp.435-453
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• 2007

KEOP (Korea Enhanced Observing Period)-2004 intensive summer observation was carried out from 20 June to 5 July 2004 over the Southwestern part of the Korean peninsula. In this study, the effects of KEOP-2004 intensive observation data on the simulation of precipitation system are investigated using KLAPS (Korea Local Analysis and Prediction System) and PSU/NCAR MM5. Three precipitation cases during the intensive observation are selected for detailed analysis. In addition to the control experiments using the traditional data for its initial and boundary conditions, two sensitivity experiments using KEOP data with and without Jindo radar are performed. Although it is hard to find a clear and consistent improvement in the verification score (threat score), it is found that the KEOP data play a role in improving the position and intensity of the simulated precipitation system. The experiments started at 00 and 12 UTC show more positive effect than those of 06 and 18 UTC. The effect of Jindo radar is dependent on the case. It plays a significant role in the heavy rain cases related to a mesoscale low over Changma front and the landing of a Typhoon. KEOP data produce more strong difference in the 06/18 UTC experiments than in 00/12 UTC, but give more positive effects in 00/12 UTC experiments. One of the possible explanations for this is that : KEOP data could properly correct the atmosphere around them when there are certain amounts of data, while gives excessive effect to the atmospheric field when there are few data. CRA analysis supports this reasoning. According to the CRA (Contiguous Rain Area) analysis, KEOP data in 00/12 UTC experiments improve only the surrounding area, resulting in essentially same precipitation system so the effects remain only in each convective cell rather than the system itself. On the other hand, KEOP data modify the precipitation system itself in 06/18 UTC experiments. Therefore the effects become amplified with time integration.

• 센서학회지
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• 제17권3호
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• pp.195-202
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• 2008

A biosensor based on the measurement of capacitance changes has been designed and fabricated for simple and realtime detection of bacteria. Compared to an impedance measurement technique, the capacitance measurement can make additional measurement circuits simpler, which improves a compatability for integration between the sensor and circuit. The fabricated sensor was characterized by detecting Escherichia coli(E. coli). The capacitance changes measured by the sensor were proportional to E. coli cell density, and the proposed sensor could detect $1{\times}10^6$ cfu/ml E. coli at least. The real-time detection was verified by measuring the capacitance every 20 minutes. After 7 hours of E. coli growth experiment, the capacitance of the sensor in the micro volume well with $4.5{\times}10^5$ cfu/ml of initial E. coli density increased by 20 pF, and that in another wells with $1.5{\times}10^6$ cfu/ml and $8.5{\times}10^7$ cfu/ml initial E. coli density increased by 56 pF and 71 pF, respectively. The proposed sensor has a possibility of the real-time detection for bacterial growth, and can detect E. coli cells with $1.8{\times}10^5$ cfu in nutrient broth in 5 hours.