Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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MEMS based capacitive biosensor for real time detection of bacterial growth

실시간 박테리아 감지를 위한 정전용량방식의 MEMS 바이오센서

  • Seo, Hye-Kyoung (School of Mechanical Engineering, Yonsei University) ;
  • Lim, Dae-Ho (School of Mechanical Engineering, Yonsei University) ;
  • Lim, Mi-Hwa (Department of Microbiology, College of Medicine, Yonsei University) ;
  • Kim, Jong-Baeg (School of Mechanical Engineering, Yonsei University) ;
  • Shin, Jeon-Soo (Department of Microbiology, College of Medicine, Yonsei University) ;
  • Kim, Yong-Jun (School of Mechanical Engineering, Yonsei University)
  • 서혜경 (연세대학교 기계공학부) ;
  • 임대호 (연세대학교 기계공학부) ;
  • 임미화 (연세대학교 의과대학 미생물학교실) ;
  • 김종백 (연세대학교 기계공학부) ;
  • 신전수 (연세대학교 의과대학 미생물학교실) ;
  • 김용준 (연세대학교 기계공학부)
  • Published : 2008.05.31
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Abstract

A biosensor based on the measurement of capacitance changes has been designed and fabricated for simple and realtime detection of bacteria. Compared to an impedance measurement technique, the capacitance measurement can make additional measurement circuits simpler, which improves a compatability for integration between the sensor and circuit. The fabricated sensor was characterized by detecting Escherichia coli(E. coli). The capacitance changes measured by the sensor were proportional to E. coli cell density, and the proposed sensor could detect $1{\times}10^6$ cfu/ml E. coli at least. The real-time detection was verified by measuring the capacitance every 20 minutes. After 7 hours of E. coli growth experiment, the capacitance of the sensor in the micro volume well with $4.5{\times}10^5$ cfu/ml of initial E. coli density increased by 20 pF, and that in another wells with $1.5{\times}10^6$ cfu/ml and $8.5{\times}10^7$ cfu/ml initial E. coli density increased by 56 pF and 71 pF, respectively. The proposed sensor has a possibility of the real-time detection for bacterial growth, and can detect E. coli cells with $1.8{\times}10^5$ cfu in nutrient broth in 5 hours.

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References

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