• 생명과학회지
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• 제28권6호
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• pp.663-670
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• 2018

이 연구는 5가지 물질로 구성된 혼합물의 항산화 및 모발 성장 촉진 효과를 확인하기 위해 수행되었다. 발효 5종 혼합물은 적양파, 늙은 호박, 검은콩, 솔잎, 검은깨, 발효 2종 혼합물은 적양파, 늙은 호박으로 구성되어있다. 항산화 효과는 DPPH 라디칼 소거활성 및 총 플라보노이드 함량으로 측정하였다. 발효 5종 혼합물은 발효 2종 혼합물보다 높은 DPPH 라디칼 소거활성과 플라보노이드 함량을 보였다. 모발 성장 효과는 생체 내 실험을 통해 검증되었다. 알칼리성 인산가수 분해효소(ALP), ${\gamma}$-글루타밀트랜스펩티다아제(${\gamma}-GT$) 및 육안적 관찰을 통해 측정하였다. 실험동물은 샘플에 따라 물(NOR), 판시딜(P-CON), 발효 5종 혼합물(5NC), 발효 2종 혼합물(2NC) 총 4군으로 나눴다. 샘플은 일정한 시간에 하루 1번 4주 동안 경구투여하였다. 5NC군에서 ALP, ${\gamma}-GT$ 활성 모두 2NC에 비해 증가하였다. 육안적 관찰에서 P-CON군은 털이 가장 길고 면도 전의 상태와 유사하였다. 5NC군은 P-CON군과 유사한 수준으로 털이 자랐다. 모든 결과에서 발효 5종 혼합물은 발효 2종 혼합물 보다 높은 항산화 효과와 모발 성장 촉진 효과를 보였다. 결론적으로 발효 5종 혼합물은 유용한 모발 성장 촉진제의 원료로 사용할 수 있음을 확인하였다.

• 대한약침학회지
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• 제18권4호
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• pp.20-25
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• 2015

Objectives: Recently, thread-embedding therapy (TET) has been widely applied in Korean medicine for cosmetic purposes such as reducing skin wrinkles. An inserted thread was reported to have induced continuous stimulation, followed by support for connective tissue regeneration. However, the potential role of TET in hair-growth has not yet been reported. Methods: We designed this study to evaluate whether TET has a hair-growth-promoting effect. C57 black 6 (C57BL/6) mice were divided into three groups: normal saline-treated, minoxidil-treated, and thread-embedded groups. Normal saline or 5% minoxidil was topically sprayed on the dorsal skin of the mice once a day for 16 days. Medical threads were embedded into the dorsal skin of the mice in a single application. Hair growth activity was evaluated by using dermoscopic and microscopic observations. Sections of the dorsal skin were stained with hematoxylin and eosin. Expressions of bromodeoxyuridine (BrdU), proliferating cell nuclear antigen (PCNA), fibroblast growth factor-7 (FGF-7), and fibroblast growth factor-5 (FGF-5) were detected by using immunohistochemical staining. A reverse transcription-polymerase chain reaction (RT-PCR) analysis was adopted to measure the messenger RNA (mRNA) expressions of FGF-7 and FGF-5. Results: TET enhanced anagen development in the hair follicles of C57BL/6 mice. The expressions of BrdU and PCNA, both of which imply active cellular proliferation, were increased by using TET. Moreover, TET increased the expression of FGF-7, an anagen-inducing growth factor, while decreasing the expression of FGF-5, an anagen-cessation growth factor, both at the protein and the mRNA levels. Conclusion: TET enhanced hair re-growth in C57BL/6 mice. TET regulated the expressions of anagen-associated growth factors and activated the proliferation of hair follicular cells in depilated skin lesions. Considering its long-lasting effect, TET may be a good alternative therapeutic for the treatment of alopecia.

• 동의생리병리학회지
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• 제21권1호
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• pp.136-144
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• 2007

Biotae Orientalis has been widely used for treatment of relaxion of smooth muscle, gastrointestinal hemorrhage and alopecia in Oriental Medicine. This experiment examined the effect of an extracts, obtained from the acetone and MeOH extracts of dried or fresh Biotae Orientalis, on hair growing activity of the C57BL/6N mice after topical application to skin. First, We examined on hair growth activity of extracts of Biotae Orientalis compare to control and 1% minoxidil groups. Second, We investigated on the number of hair follicle and mast cells after topical application of extracts of the Biotae Orientalis to skin for 16 day. Third, We investigated immunoreactive density of vascular endothelial growth factor(VEGF), protein kinase C-${\alpha}$(PKC-${\alpha}$) and stem(mast) cell factor(SCF) in skin of C57BL/6N mice by immunohistochemical methods. I fourth investigated changes of subpopulation of splenocytes and thymocytes in C57BL/6N mice for 16day using laser flow cytometry. The results were as follows : Hair growing effect of acetone and MeOH extracts of dried and fresh Biotae Orientalis was observed in 70%, 90% and 60% in hair removed skin area in 16 day respectively. Immunoreactive density of VEGF and PKC-${\alpha}$ in skin of experimental groups was weakly stained compare to control group in 10 day. Immunoreactive density of stem cell factor in skin of experimental group was heavily stained compare to control group in 10 day. Splenic TH/TC Iymphocytes of lived MeOH extracts group significantly increased compare to control group. TH cells in thymic T lymphocytes were increased compare to control group. These experiment suggest that acetone and MeOH extracts of Biotae Orientalis may be used for topical treatment of alopecia areata.

• 생명과학회지
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• 제29권4호
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• pp.455-460
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• 2019

이전 연구에서 우리는 Lactobacillus plantarum ilchiwhangchil 1785와 Lactobacillus plantarum ilchiwhangchil 2020 균주로 발효시킨 황칠나무 잎 가지 추출물의 효과적인 항균 활성을 확인하였다. 본 연구에서는 황칠나무 잎 가지 추출물을 L. plantarum ilchiwhangchil 1785와 L. plantarum ilchiwhangchil 2020 균주로 발효시킨 발효액이 인간모유두세포에 미치는 육모효과를 알아보았다. 황칠나무 잎 가지 발효 추출물의 농도가 1, $10{\mu}g/ml$ 범위에서 인간모유두세포는 세포사멸 증가 없이 유의적으로 세포 성장이 농도의존적으로 증가하였다. 또한 황칠나무 잎 가지 발효물로 처리한 세포가 대조군보다 세포성장이 활발함을 이중염색법으로 확인하였다. 더욱이 황칠나무 잎 가지 발효 추출물 처리한 세포는 처리하지 않은 세포에 비해 각각 18.84%, 23.31% 세포 이동성이 증가하였다. 고성능액체크로마토그래피(HPLC)를 이용하여 육모효과의 활성 물질을 분석하였다. 모발성장효과가 알려진 ${\beta}$-sitosterol 함량이 황칠나무 잎 가지 추출물에 비해 황칠나무 잎 가지 발효 과정에서 약 10배 증가되는 것을 확인하였다. 결과를 종합하면, 우리는 본 연구에서 황칠나무 잎 가지 추출물 발효액이 모발 성장을 촉진시킴을 확인하였다.

• KSBB Journal
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• 제27권2호
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• pp.121-126
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• 2012

In order to explore structural features of minoxidil analogs with a view of enhancing lysyl hydroxylase (LH) inhibitory activity, molecular holographic QSAR (HQSAR) and CoMSIA (comparative molecular similarity indices analysis) were performed. The results from the atomic contributions with optimized the HQSAR 6-2 model indicated that, in case of pyrimidine-1-N-oxide substituent, C2 atom of pyrimidine ring and C'3-C'4 bond of 4-piperidinol group showed the highest impact on the inhibitory activity towards LH enzyme. It was also evident from the information of the optimized CoMSIA F5 model that the inhibitory activity mainly depended on the hydrophobic field contribution (36%) and the hydrogen bond (H-bond) field contribution (49.2%) of substrate molecule. Particularly, it is predicted that the functional groups which disfavor H-bond acceptors in large space around the piperidinol group and also the functional groups which favor the H-bond acceptors at C'4 (& C'5) atom in $R_5$ group play a role for increased inhibitory activity. With this in mind, it is likely that a novel candidate having more improved inhibitory activity on hair growth could be designed in the future.

• Journal of Microbiology and Biotechnology
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• 제34권3호
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• pp.506-515
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• 2024

Primary human dermal papilla cells (HDPCs) are often preferred in studies on hair growth and regeneration. However, primary HDPCs are limited by their reduced proliferative capacity, decreased hair induction potential, and extended doubling times at higher passages. To overcome these limitations, pTARGET vectors containing human papillomavirus16 (HPV16) E6/E7 oncogenes were transfected into HDPCs and selected using G-148 to generate immortalized cells here. HPV16 E6/E7 oncogenes were efficiently transfected into primary HDPCs. Immortalized HDPC showed higher proliferative activity than primary HDPC, confirming an increased proliferation rate. Expression of p53 and pRb proteins was downregulated by E6 and E7, respectively. E6/E7 expressing HDPC cells revealed that cyclin-dependent kinase (CDK) inhibitor p21 expression was decreased, while cell cycle-related genes and proteins (CDK2 and cyclin E) and E2F family genes were upregulated. Immortalized HDPCs maintained their responsiveness to Wnt/β-catenin pathway and hair follicle formation capability, as indicated by their aggregative properties and stemness. E6/E7 immortalized HDPCs may facilitate in vitro hair growth and regeneration studies.

• Journal of Microbiology and Biotechnology
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• 제29권2호
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• pp.321-329
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• 2019

Hair loss, also known as alopecia, is a common dermatological condition of psychosocial significance; development of therapeutic candidates for the treatment of this condition is, hence, important. Silibinin, a secondary metabolite from Silybum marianum, is an effective antioxidant that also prevents various cutaneous problems. In this study, we have investigated the effect of silibinin on hair induction using three-dimensional (3D) cultured, human dermal papilla (DP) spheroids. Silibinin was found to significantly increase viability through AKT serine/threonine kinase (AKT) activation in 3D DP spheroids. This was correlated with an increase in the diameter of the 3D DP spheroids. The activation of the wingless and INT-1 (Wnt)/${\beta}$-catenin signaling pathway, which is associated with hair growth induction in the DP, was evaluated using the T cell-specific transcription factor and lymphoid enhancer-binding factor (TCF/LEF) transcription factor reporter assay; results indicated significantly increased luciferase activity. In addition, we were able to demonstrate increased expression of the target genes, WNT5a and LEF1, using quantitative real-time PCR assay. Lastly, significantly elevated expression of signature genes associated with hair induction was demonstrated in the 3D DP spheroids treated with silibinin. These results suggest that silibinin promotes proliferation and hair induction through the AKT and Wnt/${\beta}$-catenin signaling pathways in 3D DP spheroids. Silibinin can be a potential candidate to promote hair proliferation.

• 동의생리병리학회지
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• 제20권2호
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• pp.428-435
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• 2006

Cortex Mori radicis has been used as a components of antidiabetics, antiasthma and diuresis in Oriental Medicine. This experiment examined the effect of an extracts, obtained from the acetone(CM-A) and aqueous(CM-W) extracts of Cortex Mori radicis, on hair growing activity of the C57BL/6N mice after topical application to skin. We investigated the number of hair follicle and mast cells, and changes of subpopulation of splenocytes and thymocytes in skin for 16 day. The results were as follows : The Hair growing effect in experimental groups was more increased in 85%(CM-A) and 90%(CM-W) than control group(10%) in hair depilated area. The number of hair follicle in experimental groups(CM-A and CM-W) was more increased than control group. Splenic B/T lymphocytes of CM-A group were decreased compare to control group. CD4/CD8 positive TH cells in splenic T lymphocytes of CM-W group were increased compare to control group. These results suggest that CM-A and CM-W may be used in treatment of alopecia areata.

• KSBB Journal
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• 제25권3호
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• pp.230-238
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• 2010

A microbial strain having high keratinase activity was isolated from the soil of poultry factories of Gyeonggi or Chungcheong-do. The isolated strain was identified as Bacillus sp. based on its morphological and biochemical characteristics. In this study, the optimal conditions for the production of keratinase by this strain were investigated. The optimal medium composition for the keratinase production was determined to be 3.5% chicken feather as carbon source, 1.0% tryptone as organic nitrogen source, 1.0% $KNO_3$ as inorganic nitrogen source and 0.05% KCl, 0.05% $KH_2PO_4$, 0.03% $K_2HPO_4$ as mineral source and 0.01% yeast extract as growth factor. The optimal temperature and pH was $40^{\circ}C$ and 8.5 with shaking culture (200 rpm), respectively. The maximum keratinase production reached to 123 units/ml after 42 hr of cultivation under the optimal condition. When the hair was used as the sole carbon source, the maximum enzyme activity was 88 units/ml after 120 hr and in this case, the hair added in the medium was not degraded completely but got thinner than the control by 20%.

• 생명과학회지
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• 제32권3호
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• pp.196-201
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• 2022

곤충과 같은 새로운 단백질 공급원은 인간의 영양을 위해 제안됩니다. 단백질 가용성은 곤충 특성에 영향을 받을 수 있습니다. 발효를 통해 독특한 특성을 지닌 다양한 곤충 기반 성분 및 식품을 얻을 수 있습니다. 그러나 발효된 식용 곤충이 단백질 가용성에 미치는 영향은 알려져 있지 않습니다. 발효 쌍별 귀뚜라미 분말은 주로 단백질로 구성됩니다. 이전에는 귀뚜라미 분말의 경구 투여가 남성형 탈모증에서 모발 성장을 개선하는 것으로 보고 되었습니다. 본 연구의 목적은 동물모델에서 쌍별 귀뚜라미 발효분말의 모발 촉진 활성을 평가하는 것입니다. 수컷 C57BL/6 마우스(25-30 g)를 사용했습니다. 실험을 위해서 마우스의 복부 털(2×2.5 cm)을 부드럽게 제거했습니다. 그룹은 다음과 같이 처리되었습니다. 정상 급이군(쌍별귀뚜라미 분말 없음), 쌍별귀뚜라미 분말 급이군 및 발효 쌍별 귀뚜라미 분말 급이군으로 실험군은 구성 되었습니다. 음식은 11주 동안 공급하였습니다. 관찰을 수행하고 대조군 및 실험군의 결과를 비교했습니다. 대조군과 비교 하였을 때, 발효 쌍별 귀뚜라미 분말의 적용은 유의하게(p<0.01) 모발 성장을 촉진했습니다. C57BL/6 실험의 결과는 쌍별 귀뚜라미 발효 분말은 7주 후 모발 성장이 급격히 증가 되었음을 확인시켜줍니다. 급이 7주 후, 성장기의 모발 비율을 정상 급이군과 쌍별 귀뚜라미 분말 급이군에서 각각 비교한 결과, 발효 쌍별 귀뚜라미 급이군에서 약 125% 및 약 120% 증가하였습니다. 결론적으로 쌍별 귀뚜라미 발효 분말의 적용은 발모를 촉진하기 때문에 탈모증 치료의 유망한 대안으로 볼 수 있습니다. 또한 구성을 고려할 때 아미노산과 같은 미량 원소가 이러한 효과에 기여했을 수 있습니다.