KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Optimum Conditions for the Production of Keratinase by Bacillus sp. KN-517 and Application to the Degradation of Hair

Bacillus sp. KN-517에 의한 keratinase의 생산 최적 조건과 모발분해에 적용

  • Kim, Hye-Sook (Department of Bioengineering at the Postgraduate School, Konkuk University) ;
  • Shim, Kyu-Nam (Department of Microbiological Engineering, Konkuk University) ;
  • Kang, Sang-Mo (Department of Bioengineering at the Postgraduate School, Konkuk University)
  • 김혜숙 (건국대학교 생물공학과) ;
  • 심규남 (건국대학교 미생물공학과) ;
  • 강상모 (건국대학교 생물공학과)
  • Received : 2009.12.10
  • Accepted : 2010.06.13
  • Published : 2010.06.30
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Abstract

A microbial strain having high keratinase activity was isolated from the soil of poultry factories of Gyeonggi or Chungcheong-do. The isolated strain was identified as Bacillus sp. based on its morphological and biochemical characteristics. In this study, the optimal conditions for the production of keratinase by this strain were investigated. The optimal medium composition for the keratinase production was determined to be 3.5% chicken feather as carbon source, 1.0% tryptone as organic nitrogen source, 1.0% $KNO_3$ as inorganic nitrogen source and 0.05% KCl, 0.05% $KH_2PO_4$, 0.03% $K_2HPO_4$ as mineral source and 0.01% yeast extract as growth factor. The optimal temperature and pH was $40^{\circ}C$ and 8.5 with shaking culture (200 rpm), respectively. The maximum keratinase production reached to 123 units/ml after 42 hr of cultivation under the optimal condition. When the hair was used as the sole carbon source, the maximum enzyme activity was 88 units/ml after 120 hr and in this case, the hair added in the medium was not degraded completely but got thinner than the control by 20%.

Keywords

References

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