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Influence of Extracts of Biotae Orientalis on the Efficiency of Hair Growth in Hair Removed C57BL/6N Mice  

Tak, Min-Jin (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Jung, Il-Kook (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Kim, Dae-Keun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Jung, Han-Sol (Department of Oriental Pathology, College of Oriental Medicine, Woosuk University)
Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.21, no.1, 2007 , pp. 136-144 More about this Journal
Abstract
Biotae Orientalis has been widely used for treatment of relaxion of smooth muscle, gastrointestinal hemorrhage and alopecia in Oriental Medicine. This experiment examined the effect of an extracts, obtained from the acetone and MeOH extracts of dried or fresh Biotae Orientalis, on hair growing activity of the C57BL/6N mice after topical application to skin. First, We examined on hair growth activity of extracts of Biotae Orientalis compare to control and 1% minoxidil groups. Second, We investigated on the number of hair follicle and mast cells after topical application of extracts of the Biotae Orientalis to skin for 16 day. Third, We investigated immunoreactive density of vascular endothelial growth factor(VEGF), protein kinase C-${\alpha}$(PKC-${\alpha}$) and stem(mast) cell factor(SCF) in skin of C57BL/6N mice by immunohistochemical methods. I fourth investigated changes of subpopulation of splenocytes and thymocytes in C57BL/6N mice for 16day using laser flow cytometry. The results were as follows : Hair growing effect of acetone and MeOH extracts of dried and fresh Biotae Orientalis was observed in 70%, 90% and 60% in hair removed skin area in 16 day respectively. Immunoreactive density of VEGF and PKC-${\alpha}$ in skin of experimental groups was weakly stained compare to control group in 10 day. Immunoreactive density of stem cell factor in skin of experimental group was heavily stained compare to control group in 10 day. Splenic TH/TC Iymphocytes of lived MeOH extracts group significantly increased compare to control group. TH cells in thymic T lymphocytes were increased compare to control group. These experiment suggest that acetone and MeOH extracts of Biotae Orientalis may be used for topical treatment of alopecia areata.
Keywords
Biotae Orientalis; hair growth; VEGF; PKC-${\alpha}$; immune response;
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1 Maguire, H.C., Hanno, R. Disease of the hair. In Dermatology. Moshella SL, Hurley HJ(eds), 2nd ed, Vol 2, Philadelphia, Saunders Co. pp 1374-1379, 1985
2 Bergfeld, W. Diffuse hair loss in women. Cutis 22:190-195, 1987
3 Friedman, P.S. Alopecia areata and auto-immunity. Br J Dermatol 105:153-157, 1981   DOI   ScienceOn
4 Toback, C., Ajkunar, S. The emotional disturbances underlying alopecia areata, alopecia totalis and trichotillomania. Child Psychiatry Hum Dev 10:114-117, 1979   DOI   ScienceOn
5 秦漢琨. 脫髮證治. 中醫雜誌, 27(12):46, 1986
6 曾讓言. 辨證分型治療脫髮 64例的臨床體會, 江蘇中醫雜誌, 3(4):23-24, 1982
7 秦方章. 脫髮證治. 中醫雜誌, 27(12):43, 1986
8 김소영, 김중호, 채병윤. 탈모에 응용되는 神應養眞丹의 효능에 관한 실험적 연구. 외관과학회지, 4(1):43-58, 1991
9 全國韓醫科大學共同敎材編纂委員會編著. 本草學. 圖書出版 永林社, pp 440-441, 2004
10 서울大學校 天然物科學硏究所 文獻情報學 硏究室 編. 東洋醫藥科學大典. 第1 天然藥物, 修治(炮製 ), 학술편수관, p 422, 2003
11 鄧海清, 潘朝霞. 養血生發湯配合柏葉生發酊治療斑禿46例療效觀察, 新中醫 36(5):46-47, 2004
12 劉碧. 老中醫斑禿經驗錄. 實用醫技雜志 8(2):152, 2001
13 Shortman, K., Backson, H. The differentiation of T lymphocytes. I. Proliferation kinetics and interrelationships of subpopulations of mouse thymus cells. Cell Immunol 12:230-246, 1974   DOI   ScienceOn
14 陳貴廷. 實用中醫結合診斷治療學. 一中社, p 1508, 1992
15 葉坤照. 側柏酊治療禿發 160例小結. 中華醫學雜志 53(8):459, 1973
16 Tomita, B., Hirose, Y., Nakatsuka, T. Terpenoids XVI . New constituents of Biota orientalis (Thuga orientaljs). Tetrahedron Lett, 7:843-848, 1968
17 Kosuge, T., Yokota, M., Sugiyama, K., Saito, M., Iwata, Y., Nakura, M., Yamamoto, T. Studies on anticancer principles in Chinese Medicines. II. Cytotoxic principles in Biota orientalis (L.) Endl, and Kaempheria galanga L.. Chem Phann Bull, 33:5565-5567, 1985   DOI   ScienceOn
18 劉愛如 等. 山東地區側柏葉止血和抑菌作用比較, 山東中醫學院學報, 19(1):47, 1995
19 唐春萍 等. 側柏葉乙酸乙酯提取物對豚鼠離體氣管平滑肌的作用, 中草藥, 30(4):278, 1999
20 Goldman, C.K., Tsai, J.C., Soroceanu, L., Gillespie, G.Y. Loss of vascular endothelial growth factor in human alopecia hair follicles. J Invest Dermatol(Suppl) 104:18-20, 1995   DOI   ScienceOn
21 Selmanowilz, V., Victor, S., Warburton, D., Orentreich, N. Fingerprints arches in alopecia areata. Arch Dermatol 110:570-571, 1974   DOI   ScienceOn
22 李時珍. 校正本 本草綱目. 第22卷-第33卷, 醫聖堂, p 1915, 1993
23 오덕환. 측백엽과 석창포추출물의 항균학적 성질. 강원대학교 농업과학 연구소 논문집, 11:47-55, 2000
24 李元文, 張韋川. 名醫臨證經驗. 北京, 人民衛生出版社, p 572, 2001
25 Yang, H.O., Suh, D.Y., Han, B.H. Isolation and characterization of PAF receptor binding antagonists from Biota Orientalis. Planta Med, 61:37-40, 1995   DOI   ScienceOn
26 Peters, E.M., Maurer, M., Botchkarev, V.A., Jensen, K., Welker, P., Scott, G.A., Paus, R. Kit is expressed by epithelial cells in vivo. J Invest Dermatol 121:976-984, 2003   DOI   ScienceOn
27 Tomita, B., Hirose, Y., Nakatsuka, T. Terpenoids XIX. New constituents of Biota orientalis (Thuga orientaljs). Mokuzai Gak-kaishi, 15:46, 1969
28 최근희 외 11인. 모발과학. 수문사, pp 138-140, 2001
29 金達鎬. 黃帝內經注解補注素問. 醫聖堂, p 234, 2001
30 Tomita, B., Hirose, Y. Terpenoids XXIII. taxonomy of Cupressaceae. 2. sesqui-terpenes of Biota orientalis wood. Mokuzai Gakkaishi, 15:337-340, 1969
31 楊思澍. 中醫臨床大全. 大星出版社, p 912, 1988
32 金達鎬. 黃帝內經注解補注素問. 醫聖堂, p 908, 2001
33 Andrew, P., Warren, R., Najma, H., Nizam, U., Khan, M.L., Rahman, W. Biflavonyl pigments from Thuja orientalis (Cupressaceae). Phytochemistry, 9:1897-1898, 1970   DOI   ScienceOn
34 Detmar, M. Molecular regulation of angiogenesis in the skin. J Invest Dermatol, 106:207-208, 1996   DOI   ScienceOn
35 Li, L.F., Guo, J., Gao, Z.F. Overexpression of skin protein kinase C-alpha in anagen hair follicles during induced growth of mouse hair. Clin Exp Dermatol 28:429-433, 2003   DOI   ScienceOn
36 史玉俊. 側柏葉中的抗血小板活性因子 pinusolidic酸, 中草藥, 29(11):790, 1998
37 Muller, H.K., Rook, A.J., Kubba, R. Immunohistology and utoantibody studies in alopecia areata. Br J Dermatol 102:609-610, 1980   DOI   ScienceOn
38 中藥大辭典 編纂委員會. 完譯中藥大辭典. 鼎談, 第9卷, pp 4350-4354, 1997
39 朱仁康. 脫髮證治. 中醫雜誌, 2(12):46, 1986
40 임사비나, 최규동, 김수경. 탈모에 대한 동서의학적 고찰. 경산대학교 제한동의학술원 논문집, 4(1):699-710, 1999
41 Kozlowska, U., Blume-Peytavi, U., Kodelja, V., Somme,r C., Goerdt, S., Majewski, S., Jablonska, S., Orfanos, C.E. Expression of vascular endothelial growth factor(VEGF) in various compartments of the human hair follicle. Arch Dermatol Res 290:661-668, 1998   DOI   ScienceOn
42 홍석훈, 노석선. 모발탈락의 병인 및 치료법에 대한 문헌적 고찰. 외관과학회지, 9(1):43-65, 1996
43 노현찬, 노석선. 苦蔘추출물이 모발성장 촉진 및 면포억제에 미치는 영향. 대한안이비인후피부과학회지, 15(1):96-126, 2002
44 金達鎬. 黃帝內經注解補注素問. 醫聖堂, p 128, 2001
45 송순기, 이명종. 안드로겐성 탈모증의 두피재활에 관한 문헌적 고찰. 한의정보학회지, 5(1):162-176, 1999