Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Establishment and Characterization of Immortalized Human Dermal Papilla Cells Expressing Human Papillomavirus 16 E6/E7

  • Seonhwa Kim (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Kyeong-Bae Jeon (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Hyo-Min Park (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Jinju Kim (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Chae-Min Lim (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Do-Young Yoon (Department of Bioscience and Biotechnology, Konkuk University)
  • Received : 2023.10.25
  • Accepted : 2023.11.11
  • Published : 2024.03.28
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Abstract

Primary human dermal papilla cells (HDPCs) are often preferred in studies on hair growth and regeneration. However, primary HDPCs are limited by their reduced proliferative capacity, decreased hair induction potential, and extended doubling times at higher passages. To overcome these limitations, pTARGET vectors containing human papillomavirus16 (HPV16) E6/E7 oncogenes were transfected into HDPCs and selected using G-148 to generate immortalized cells here. HPV16 E6/E7 oncogenes were efficiently transfected into primary HDPCs. Immortalized HDPC showed higher proliferative activity than primary HDPC, confirming an increased proliferation rate. Expression of p53 and pRb proteins was downregulated by E6 and E7, respectively. E6/E7 expressing HDPC cells revealed that cyclin-dependent kinase (CDK) inhibitor p21 expression was decreased, while cell cycle-related genes and proteins (CDK2 and cyclin E) and E2F family genes were upregulated. Immortalized HDPCs maintained their responsiveness to Wnt/β-catenin pathway and hair follicle formation capability, as indicated by their aggregative properties and stemness. E6/E7 immortalized HDPCs may facilitate in vitro hair growth and regeneration studies.

Keywords

Acknowledgement

This study was supported by the National Research Foundation of Korea (grant number 2021R1A2C3014577) and Konkuk University Researcher Fund in 2023. We thank our colleagues for their assistance with this study.

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