• Asian Pacific Journal of Cancer Prevention
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• 제15권12호
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• pp.5055-5061
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• 2014

In this study, an anti-oxidant and anti-tumor protein Latcripin-3 of Lentinula edodes C91-3 was expressed in Escherichia coli. for the first time. According to the cDNA library, the full-length gene of Latcripin-3 was cloned by the methods of 3'-full rapid amplification of cDNA Ends (RACE) and 5'-full RACE. The structural domain gene of Latcripin-3 was inserted into the pET32 a(+). The functional protein of Latcripin-3 was expressed in Rosetta-gami (DE3) E. coli, evaluated by Western blotting and mass spectrometry. DPPH testing showed that the protein Latcripin-3 can scavenge free radicals remarkably well. The activity of functional protein Latcripin-3 on A549 cells was studied with flow cytometry and the MTT method. The MTT assay results showed that there was a decreases in cell viability in a dose-dependent and time-dependent manner in protein Latcripin-3 treated groups. Flow cytometry demonstrated that Latcripin-3 can induce apoptosis and block S phase dramatically in human A549 lung cancer cells as compared to the control group. At the same time, the cell ultrastructure observed by transmission electron microscopy supported the results of flow cytometry. This research offers new insights and advantages for identifying anti-oxidant and anti-tumor proteins.

• 한국광학회지
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• 제14권5호
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• pp.504-508
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• 2003

최근 전자산업계에 새롭게 널리 생산되는 마이크로 전자부품들은 왜곡이 최소화된 정밀한 외관 형상을 갖도록 제조되고 관리되지만, 측정 대상의 표면이 가시광 영역에서 광산란되는 특징을 가짐으로 인해, 기존의 피죠나 마이켈슨 형태의 비교간섭법으로는 고정밀의 삼차원 형상측정이 용이하지 아니하였다. 본 논문에서는 광섬유를 이용한 새로운 개념의 점회절 간섭계를 제안하고, 이를 광산란 거친표면의 대표적인 제품인 칩패키지와 실리콘 웨이퍼의 삼차원 형상 측정에 적용하였다. 측정결과 66 mm 측정영역에서 측정 형상오차 PV(peak-to-valley value) 5.6 $\mu\textrm{m}$, 분산값($\sigma$) 1.5 $\mu\textrm{m}$를 획득함으로써 기존의 비교 간섭 측정법에 비해 더욱 향상된 측정 정밀도를 획득하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제35권1호
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• pp.1-6
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• 2009

DNA double-strand breaks (DSBs) occur commonly in the all living and in cycling cells. They constitute one of the most severe form of DNA damage, because they affect both strand of DNA. DSBs result in cell death or a genetic alterations including deletion, loss of heterozygosity, translocation, and chromosome loss. DSBs arise from endogenous sources like metabolic products and reactive oxygen, and also exogenous factors like ionizing radiation. Defective DNA DSBs can lead to toxicity and large scale sequence rearrangement that can cause cancer and promote premature aging. There are two major pathways for their repair: homologous recombination(HR) and non-homologous end-joining(NHEJ). The HR pathway is a known "error-free" repair mechanism, in which a homologous sister chromatid serves as a template. NHEJ, on the other hand, is a "error-prone" pathway, in which the two termini of the broken DNA molecule are used to form compatible ends that are directly ligated. This review aims to provide a fundamental understanding of how HR and NHEJ pathways operate, cause genome instability, and what kind of genes during the pathways are associated with head and neck cancer.

• JSTS:Journal of Semiconductor Technology and Science
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• 제17권1호
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• pp.129-140
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• 2017

In this paper, a $4{\times}32$-channel neural recording system capable of acquiring neural signals is introduced. Four 32-channel neural recording ICs, complex programmable logic devices (CPLDs), a micro controller unit (MCU) with USB interface, and a PC are used. Each neural recording IC, implemented in $0.18{\mu}m$ CMOS technology, includes 32 channels of analog front-ends (AFEs), a 32-to-1 analog multiplexer, and an analog-to-digital converter (ADC). The mid-band gain of the AFE is adjustable in four steps, and have a tunable bandwidth. The AFE has a mid-band gain of 54.5 dB to 65.7 dB and a bandwidth of 35.3 Hz to 5.8 kHz. The high-pass cutoff frequency of the AFE varies from 18.6 Hz to 154.7 Hz. The input-referred noise (IRN) of the AFE is $10.2{\mu}V_{rms}$. A high-resolution, low-power ADC with a high conversion speed achieves a signal-to-noise and distortion ratio (SNDR) of 50.63 dB and a spurious-free dynamic range (SFDR) of 63.88 dB, at a sampling-rate of 2.5 MS/s. The effectiveness of our neural recording system is validated in in-vivo recording of the primary somatosensory cortex of a rat.

• 복식문화연구
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• 제18권6호
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• pp.1153-1164
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• 2010

The objective of this study is to analyze the coordination images seen in comprehensive fashion items and their features. This will be done from the viewpoint of both the creativity as well as functional characteristics of items and images. For the method of study, I explored the idea of "snood" style, analyzing its features of 42 pictures appearing from the 2006 S/S to 2010 F/W collection. It is important to note that "snood" style has the characteristics of both a muffler and turtleneck. With looping design connected at both ends, it can be placed around the neck or head, creating the image of wearing a hood. After having examined the selected data and pictures, one can largely divide the exclusive high-fashion image categories into three types: feminine, avant-garde, and finally, active & functional sportive image. First, the orthodox image is widely accepted by most as it has forever evolved within the original tradition of the practically functional muffler(scarf). Second, since the metamorphic image tends to lend itself to free ideas, you can wear a snood around the shoulders like a collar. Worn together with the same type of clothing, the snood can be seen as an effective suit. Third, the aim of image emphasis is to highlight certain points, or make some features more noticeable, as a means of possibly attracting more interest and attention. The image of snood arises out of the use of shapes, colors, and other accessory parts. As mentioned earlier, snood stands out as an independent item instead of just being an accessory to clothing. Its primary function as a style coordinator is emphasized in order to create more distinctive fashion images. Through this study, I thereby intend to provide fashion style data on the latest trends, and high-fashion codes of snood coordination.

• 한국지반공학회지:지반
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• 제12권5호
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• pp.63-78
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• 1996

퇴적연암의 변형특성을 조사하기 위하여 여러 종류의 실내시험과 원위치시험결과를 비교검토 하였다. 초음파측정시험 등의 실내시험에서 불구속 시료의 탄성계수는 원위치유효토피압에 해당하는 압밀구속압으로 구속한 시료의 탄성계수 보다 현저히 작았다. 또 삼축압축시험에서 재하피 스톤의 축변위로 부터 구한 축변형률은 시료 상.하단에서의 오차(bedding error)로 인하여 정확히 측정할 수 없고, 전체적으로 신뢰할 수 없다. 따라서 시료측면에서 직접 변형률을 측정하는 국소변형측정장치를 이용하여 0.001% 이하에서 약 1%까지 연속적으로 위와 같은 오차를 제거한 축변형률을 얻을 수 있었고, 0.00% 이하 축변형률에서 정의된 탄성계수 Emax는 원위치탄성 파속도시험으로 얻어진 탄성계수 Er와 거의 일치하였다. 변형률이 0.01%에 도달하기 이전에 변형은 비선형성을 보이기 시작하고, 실제 원위치 작용하중 범위에서 예상할 수 있는 최대변형률 0.1%에서의 할선탄성계수 Esec는 Emax의 1/2보다 여전히 큰 값을 보인다. 위와 같은 내용을 골자로 하여 정밀측정실내시험 결과에 따른 변형특성과 현장거동 및 원위치측정결과의 비교검토를 통해 변형률레벨을 고려한 원위치 탄성계수 추정법을 제안하였다.

• BMB Reports
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• 제43권3호
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• pp.176-181
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• 2010

The primary sigma factor ($\sigma^{A}$) of Staphylococcus aureus, a potential drug target, was little investigated at the structural level. Using an N-terminal histidine-tagged $\sigma^{A}$ (His-$\sigma^{A}$), here we have demonstrated that it exits as a monomer in solution, possesses multiple domains, harbors primarily $\alpha$-helix and efficiently binds to a S. aureus promoter DNA in the presence of core RNA polymerase. While both N- and C-terminal ends of His-$\sigma^{A}$ are flexible in nature, two Trp residues in its DNA binding region are buried. Upon increasing the incubation temperature from 25$^{\circ}$ to 40$^{\circ}C$, $\sim$60% of the input His-$\sigma^{A}$ was cleaved by thermolysin. Aggregation of His-$\sigma^{A}$ was also initiated rapidly at 45$^{\circ}C$. From the equilibrium unfolding experiment, the Gibbs free energy of stabilization of His-$\sigma^{A}$ was estimated to be +0.70 kcal $mol^{-1}$. The data together suggest that primary sigma factor of S. aureus is an unstable protein. Core RNA polymerase however stabilized $\sigma^{A}$ appreciably.

• 한국해안해양공학회지
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• 제19권5호
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• pp.484-491
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• 2007

규칙파와 수면아래 잠긴 수평판 사이의 비선형 상호작용을 규명하기 위하여 수치파동수조를 이용하여 수치 실험을 수행하였다. 수치모델로 비선형 포텐셜 이론을 근거로 한 고차 경계요소법과 Navier Stokes 방정식과 엄밀한 자유표면 경계조건식을 푸는 CADMAS-SURF을 사용하였다. 두 모델 모두 수평판 위의 천수역에서 발생하는 고차 조화항들을 예측할 수 있으며 점성효과를 포함하는 CADMAS-SURF는 수평판 양단에서 발생하는 와류와 박리에 의한 고차 조화항의 발생을 재현할 수 있다. 수평판의 잠긴 깊이와 길이 변화에 따른 반사율과 투과율을 Patarapanich and Cheong(1989)의 모형실험결과와 비교하였고 서로 잘 일치함을 확인하였다. 수평판의 길이가 길어지고 잠긴 깊이가 얕아질수록 입사파의 에너지가 고차 조화항으로 더 많이 전이됨을 확인하였다.

• Structural Engineering and Mechanics
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• 제35권6호
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• pp.677-697
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• 2010

This paper focuses on geometrically non-linear static analysis of a simply supported beam made of hyperelastic material subjected to a non-follower transversal uniformly distributed load. As it is known, the line of action of follower forces is affected by the deformation of the elastic system on which they act and therefore such forces are non-conservative. The material of the beam is assumed as isotropic and hyperelastic. Two types of simply supported beams are considered which have the following boundary conditions: 1) There is a pin at left end and a roller at right end of the beam (pinned-rolled beam). 2) Both ends of the beam are supported by pins (pinned-pinned beam). In this study, finite element model of the beam is constructed by using total Lagrangian finite element model of two dimensional continuum for a twelve-node quadratic element. The considered highly non-linear problem is solved by using incremental displacement-based finite element method in conjunction with Newton-Raphson iteration method. In order to use the solution procedures of Newton-Raphson type, there is need to linearized equilibrium equations, which can be achieved through the linearization of the principle of virtual work in its continuum form. In the study, the effect of the large deflections and rotations on the displacements and the normal stress and the shear stress distributions through the thickness of the beam is investigated in detail. It is known that in the failure analysis, the most important quantities are the principal normal stresses and the maximum shear stress. Therefore these stresses are investigated in detail. The convergence studies are performed for various numbers of finite elements. The effects of the geometric non-linearity and pinned-pinned and pinned-rolled support conditions on the displacements and on the stresses are investigated. By using a twelve-node quadratic element, the free boundary conditions are satisfied and very good stress diagrams are obtained. Also, some of the results of the total Lagrangian finite element model of two dimensional continuum for a twelve-node quadratic element are compared with the results of SAP2000 packet program. Numerical results show that geometrical nonlinearity plays very important role in the static responses of the beam.

• Journal of Plant Biotechnology
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• 제32권2호
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• pp.135-138
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• 2005

더덕 자엽절편을 1 mg/L 2,4-D가 첨가된 MS기본배지에 배양하여 배발생캘러스를 얻었고, 호르몬이 첨가되지 않은 MS액체배지에서 구형기의 체세포배를 얻었다. 구형기에서 초기 심장형기로 발달할 때 자엽의 시원세포는 전형성층조직으로부터 분화되기 시작 하였으며, 하배축에서 관찰되는 원통형 전형성층대는 2개의 자엽을 형성할 경우 2개의 전형성층대가, 3개의 자엽은 3개의 전형성층대가, 4개의 자엽은 4개의 전형층대가 각각 독립적으로 분화되어 자엽절과 자엽부위까지 연결되어 있었다. 만약 구형기 체세포배에서 자엽시원세포가 원통형으로 분화될 경우 합생자엽을 갖는 체세포배가 형성되었다.