• Title/Summary/Keyword: fermenter

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Pseudomonas aeruginosa BYK-2에 의한 생물유화제에 발효생산

  • Kim, Hak-Ju;Lee, Gyeong-Mi;Jeong, Hye-Seong;Kim, Bong-Jo;Gang, Yang-Sun;Gong, Jae-Yeol
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.263-266
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    • 2000
  • The purified biosurfactant $3.16g/{\ell}$ was obtained after cultivation for 104hr at $25^{\circ}C$ with an optimal agitation speed of 200rpm, an aeration rate of 2vvm in a $14{\ell}$ fermenter containing $5.5{\ell}$ of LB medium and 1%(w/v) olive oil as a carbon source. For the kinetic studies, the optimal substrate concentration was analyzed on different olive oil concentrations(0.1, 0.5, 1.0, 1.5, 2.0%(w/v)) and optimal culture conditions(MLBM, 200rpm, 2vvm at $25^{\circ}C$) in a $14{\ell}$ jar fermenter. The results obtained indicate that $K_s$=0.0086 $g/{\ell}$, $q_s$= 0.664 $g/g{\cdot}h$, $q_p$= $4.2{\times}10^{-3}$ $g/g{\cdot}h$, and ${\mu}_{max}$ was determined as $0.1449h^{-1}$.

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비스판 변이주에 의한 활성아포 생산성 증가

  • Jeon, Gyeong-Dong;Lee, Gwang-Ho;Kim, Won-Seok;Baek, Hyeon-Dong
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.275-278
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    • 2000
  • Bacillus polyfermenticus SCD which is commonly called as 'Bispan strain' has been appropriately used for the treatment of long-term intestinal disorders, since the live strains in the form of active endospores can successfully reach the target intestine. The goal of this study were to get the novel bispan mutant strain and to get higher spore number of bispan mutant in submerged fermentation. Bispan mutant KD21 was obtained using NTG mutagenesis of bispan strain, and bispan mutant was cultivated in 5 L jar fermenter. In this study, productivity and sporulation rate of the bispan mutant KD2l showed approximately 77% increase when compared with bispan strain.

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회분식과 유가식 배양에 의한 Motierella alpina로부터의 Arachidonic acid의 생산

  • Hwang, Byeong-Hui;Park, Chang-Yeol;Yu, Yeon-U
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.378-381
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    • 2001
  • In the batch culture experiments, the addition of $MnSO_4$, was examined in flask culture, and then the optimal amounts of $MnSO_4$ was investigated in 2.5- L jar-fermenter. As a results, 0.005% $MnSO_4$ was found to enhance the ARA yield of 1.14- fold. Also the addition of $KH_2PO_4_4$ was investigated in 2.5-L jar-fermenter and the ARA yield was enhanced 1.20-fold. Fed batch culture study shown a relatively high productivity of cell mass (62.1 g/L) and ARA content (12.0 g/L) when 14% ammonia solution were alternatively used to control the pH and nitrogen source in the cultivation period.

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Morphological and rheological properties of culture broth of Cephalosporium acremonium M25

  • Lim, Jung-Soo;Kim, Jin-Hee;Kim, Chongyoup;Kim, Seung-Wook
    • Korea-Australia Rheology Journal
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    • v.14 no.1
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    • pp.11-16
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    • 2002
  • Cephalosporium acremonium is a filamentous microorganism producing cephalosporin C. The morphological differentiation of C. acremonium in submerged culture is closely related with the rheological properties of culture broth and production of cephalosporin C. In this study, the rheological and morphological properties of culture broth of C. acremonium were investigated. In the seed broths of shake-flask and fermenter culture, the Herschel-Berkley equation was in excellent agreement with experimental results in the whole range of shear rate. In the seed broths of shake-flask culture, morphological differentiation into arthrospores affected to changes of apparent viscosity. But results in the fermenter culture, morphological factors such as mean hyphal thickness and the number of tips gave more effect on changes of apparent vitacosity than differentiation into arthrospores. Overall, it suggested that the morphological parameters measured by image analysis can be used as a good parameter to indicate the rheological properties of culture broth of C. acremonium M25.

Enhancement of L-Threonine Production by Controlling Sequential Carbon-Nitrogen Ratios during Fermentation

  • Lee, Hyeok-Won;Lee, Hee-Suk;Kim, Chun-Suk;Lee, Jin-Gyeom;Kim, Won-Kyo;Lee, Eun-Gyo;Lee, Hong-Weon
    • Journal of Microbiology and Biotechnology
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    • v.28 no.2
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    • pp.293-297
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    • 2018
  • Controlling the residual glucose concentration is important for improving productivity in $\text\tiny{L}$-threonine fermentation. In this study, we developed a procedure to automatically control the feeding quantity of glucose solution as a function of ammonia-water consumption rate. The feeding ratio ($R_{C/N}$) of glucose and ammonia water was predetermined via a stoichiometric approach, on the basis of glucose-ammonia water consumption rates. In a 5-L fermenter, 102 g/l $\text\tiny{L}$-threonine was obtained using our glucose-ammonia water combined feeding strategy, which was then successfully applied in a 500-L fermenter (89 g/l). Therefore, we conclude that an automatic combination feeding strategy is suitable for improving $\text\tiny{L}$-threonine production.

The Characteristics of SBR Treatment with Different Types of Piggery Wastewater (축산폐수성상에 따른 SBR 처리특성)

  • Jun, Byong-Hee;Poo, Kyung-Min;Kim, Yang-Hoon;Im, Jeong-Hoon;Kim, Chang-Won
    • Journal of Korean Society on Water Environment
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    • v.18 no.4
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    • pp.435-440
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    • 2002
  • A pilot scale SBR (effective volume, $20m^3$) for the treatment of piggery wastewater treatment was performed with three different kinds of wastewater; fermenter effluent, scraper type and slurry type. The react phase in SBR was performed by sub-cycle operation consisting of repeated short cycle of anoxic-aerobic step. The fermenter effluent was characterized by the rapid nitrification and $NO_X-N$ accumulation due to depletion of organic matter in wastewater. The scraper type wastewater showed appropriate nitrogen removal efficiency, however, a poor response capacity for high loading rate often resulted in increased nitrogen concentration in effluent. Moreover, severe P release was the most serious problem in scraper type wastewater. SBR treated slurry type wastewater with high nitrogen removal efficiency to satisfy effluent quality requirement. It was thought that high concentration of organic matter in slurry made it possible to uptake P during SBR operation, where P concentration of 140mgP/l was decreased to 8mgP/l. As results, SBR was suitable to treat slurry type wastewater which has been discharged to the ocean till now.

On-line Measurement of Cooling Rate of a Fermenter and its Application for Fed-batch Control (발효조의 냉각량 연속 측정 및 이를 이용한 유가배양제어)

  • Heo, Won;Hong, Gun-Pyo
    • KSBB Journal
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    • v.18 no.5
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    • pp.418-423
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    • 2003
  • A laboratory jar fermenter was modified to measure the duration for cooling water supply and the temperatures of the coolant at the inlet and outlet of water jacket. Successful operation of temperature control and on-line measurement was achieved by adjusting optimum parameters of the Proportion-Integral-Derivative temperature controller. The variables measured on-line were used to estimate cooling rates from empirical equations comprised of the time period of cooling water supply and the temperatures of coolant. The measured cooling rate showed a good correlation to the specific growth rate during batch cultivation of E. coli. Cooling rate was measured and applied to programmed cell growth in a fed-batch cultivations. Three fed-batch cultivations were demonstrated by feeding substrate to follow the programmed cooling rates increasing exponentially.

A Bibliographical Study on the Manufacturing Procedure of Sogog-Ju (소국주양조에 관한 문헌적 고찰)

  • 남궁석;이성우;배상면
    • Journal of the East Asian Society of Dietary Life
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    • v.2 no.1
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    • pp.83-94
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    • 1992
  • As the result of the research on the manufacturing procedure of Sogog-ju in the classical literature(35 kinds), it was found that Sogog-ju is usually brewed on the first Boar day of January and Sogog-ju base is added to it in about 7days and then the fermentation of Sogog-ju comes to end in about 21 days. The material grains out of 48 items, used in the first step of brewing Sogog-ju, are nonglutnous rice 47 items(98%), flour 20items(42%), somnuruk 9 items(19%) out of nuruk (fermenter) 48 items (100%) and nuruk water 5 items(17%), and flour 3 items(6%) are used, but fermenter nuruk is not used. When the grains are used in the first step of brewing, they are usually in the form of gruel and rice cake, and in the second step of brewing, they are usually made into steam water soaked rice. Nuruk, Which is largely used in the first step, is usually put after the processed grains get cold, but in the second step of brewing, it may be put into the still warm grains. There are hardly any cases when nuruk is used in conspicously small quantity.

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Effect of Culture Conditions on Canthaxanthin Production by Dietzia natronolimnaea HS-1

  • Khodaiyan Khodaiyan;Faramarz Faramarz;Razavi Seyed Hadi;Emam-Djomeh Zahra;Mousavi Seyed Mohammad Ali;Hejazi Mohammad Amin
    • Journal of Microbiology and Biotechnology
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    • v.17 no.2
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    • pp.195-201
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    • 2007
  • This study investigated the effects of various culture parameters (carbon sources, temperature, initial pH of culture, NaCl concentration, and light) on the growth and canthaxanthin production by Dietzia natronolimnaea HS-1. The results showed that the most effective carbon source for growth and canthaxantin production was glucose, and the best pH and temperature were 7 and $31^{\circ}C$, respectively. In addition, the biomass and canthaxanthin production increased in a medium without NaCl and in the presence of light. Under the optimized conditions, the maximum biomass, total carotenoid, and canthaxanthin production were $6.12{\pm}0.21g/l,\;4.51{\pm}0.20mg/l,\;and\;4.28{\pm}0.15mg/l$, respectively, in an Erlenmeyer flask system, yet increased to 7.25 g/l, 5.48 mg/l, and 5.29 mg/l, respectively, in a batch fermenter system.

Optimization of Large Scale Culture Conditions of Bacillus ehimensis YJ-37 Antagonistic to Vegetables Damping-off Fungi (채소류 모잘록병균에 길항하는 Bacillus ehimensis YJ-37의 대량배양 최적조건)

  • 주길재;김진호
    • Journal of Life Science
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    • v.12 no.3
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    • pp.242-249
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    • 2002
  • The optimal culture conditions in 500$m\ell$ flask suture, 5$\ell$ jar fermenter and 2,000 $\ell$ large stale culture were investigated to maximize the production of antibiotic on Rhizoctonia solani AC4, the causal agent of vegetables damping-off, by the strain Bacillus ehimensis YJ-37. Starch (1.5%) as a carbon source, peptone (0.6%) as a nitrogen source and MgC1$_2$(0.15%) as a metal ion in the medium containing N $a_2$HP $O_4$(0.3%) showed the highest production of the antibiotic(s) in a rotary shake (200 rpm). Optimal initial pH of the culture medium, culture temperature and culture time for the antibiotic(s) production were pH 8.0, 32$^{\circ}C$ and 54hrs, respertively. Under the optimal renditions in flask culture, cell growth and antifungal activity (clear zone size) were 1.42 $\times$ 10$^{8}$ cfu/$m\ell$ (21g-DCW/ $\ell$) and 13.9 mm, respectively. In 5 $\ell$ jar fermenter (medium 3 $\ell$), optimal air flow, agitation speed and culture time for the antibiotic(s) production were 2 vvm, 200 rpm and 48hrs, respectively. Under the optimal conditions in 5 $\ell$ jar fermenter, tell growth and antifungal activity were 2.06 $\times$ 10$^{8}$ cfu/$m\ell$ (30g-DCW/ $\ell$) and 13.4 mm, respectively. Under the culture conditions of air flow (30 vvm) and agitation speed (200 rpm) at 32$^{\circ}C$ for 10 days in 2,000 $\ell$ large scale culture (medium 1,800 $\ell$, pH 8.0), cell growth and antifungal activity were 0.81$\times$10$^{8}$ cfu/$m\ell$ (12g-DCW/ $\ell$) and 8.6 mm, respectively.