KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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On-line Measurement of Cooling Rate of a Fermenter and its Application for Fed-batch Control

발효조의 냉각량 연속 측정 및 이를 이용한 유가배양제어

  • Heo, Won (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Hong, Gun-Pyo (School of Biotechnology and Bioengineering, Kangwon National University)
  • 허원 (강원대학교 바이오산업공학부) ;
  • 홍건표 (강원대학교 바이오산업공학부)
  • Published : 2003.10.01
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Abstract

A laboratory jar fermenter was modified to measure the duration for cooling water supply and the temperatures of the coolant at the inlet and outlet of water jacket. Successful operation of temperature control and on-line measurement was achieved by adjusting optimum parameters of the Proportion-Integral-Derivative temperature controller. The variables measured on-line were used to estimate cooling rates from empirical equations comprised of the time period of cooling water supply and the temperatures of coolant. The measured cooling rate showed a good correlation to the specific growth rate during batch cultivation of E. coli. Cooling rate was measured and applied to programmed cell growth in a fed-batch cultivations. Three fed-batch cultivations were demonstrated by feeding substrate to follow the programmed cooling rates increasing exponentially.

발효조의 냉각수 공급 제어 신호와 냉각수의 유출입 지점의 온도를 온라인으로 측정하여 이 값들로부터 냉각량을 추정할 수 있는 실험식을 얻었다. 회분식 배양을 통하여 냉각량이 온라인으로 측정될 수 있음을 확인하였고 기질의 재투입시 냉각량의 변화를 통하여 유가배양의 제어변수로 활용할 수 있음을 점검하였다. 미리 프로그램된 목표 냉각량의 증가곡선에 따라 냉각량이 증가되면서 세포가 자라도록 유가배양을 실시하였다. 본 연구를 통하여 냉각량의 온라인 측정방법을 제시하였고 이를 유가배양 제어변수로 활용하여 세포의 성장속도를 조절할 수 있음을 보였다.

Keywords

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