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On-line Measurement of Cooling Rate of a Fermenter and its Application for Fed-batch Control  

Heo, Won (School of Biotechnology and Bioengineering, Kangwon National University)
Hong, Gun-Pyo (School of Biotechnology and Bioengineering, Kangwon National University)
Publication Information
KSBB Journal / v.18, no.5, 2003 , pp. 418-423 More about this Journal
Abstract
A laboratory jar fermenter was modified to measure the duration for cooling water supply and the temperatures of the coolant at the inlet and outlet of water jacket. Successful operation of temperature control and on-line measurement was achieved by adjusting optimum parameters of the Proportion-Integral-Derivative temperature controller. The variables measured on-line were used to estimate cooling rates from empirical equations comprised of the time period of cooling water supply and the temperatures of coolant. The measured cooling rate showed a good correlation to the specific growth rate during batch cultivation of E. coli. Cooling rate was measured and applied to programmed cell growth in a fed-batch cultivations. Three fed-batch cultivations were demonstrated by feeding substrate to follow the programmed cooling rates increasing exponentially.
Keywords
Calorimeter; metabolic heat; cooling rate; fed-batch;
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  • Reference
1 Quantatification of small enthalpic differences in anaerobic microbial metabolism - a calorimetry supported approach /
[ Meiser-Schneiders M.;F.Schafer ] / Thermochimica Acta   ScienceOn
2 Calorimetric identification of several strains of lactic acid bacteria /
[ Fujita,T.;P.R.Monk;I.Wasdui ] / J. Dairy Res.
3 /
[ Roskams,J.;L.Rodgers ] / LAb Ref: A handbook of recipes, reagents, and other reference tools for use at the bench
4 Application of dynamic calorimetry for monitoring fermentation processes /
[ Mou,D.G.;C.I.Cooney ] / Biotechnol. Bioeng.   ScienceOn
5 The application of a novel heat flux calorimeter for studying growth of Escherichia coli W in aerobic batch culture /
[ Marison,I.W.;U.von Stockar ] / Biotechnol. Bioeng.   ScienceOn
6 Calorimetric control of fed-batch fermentations /
[ Randolph,T.W.;I.W.Marison;D.E.Martens;U.von stockar ] / Biotechnol. Bioeng.
7 /
[ Gayford,C.G. ] / Energy and cells
8 A calorimetric investigation of the aerobic cultivation of Kulyveromyces fragilis on various substrates /
[ Marison,I.W.;U.von Stockar ] / Enzyme Microb. Technol.   ScienceOn
9 Monitoring and control of the physiological state of cell culture /
[ Konstantinov,K.B. ] / Biotechnol. Bioeng.
10 Calorimeteric investigation of aerobic fermentation /
[ Birou,B.;I.W.Marison;U.von Stockar ] / Biotechnol. Bioeng.   ScienceOn
11 Heat evolution during the microbial process estimation /
[ Luong,J.H.T.;B.Volesky ] / Adv. Biochem. Eng. Biotechnol.
12 Determination of the maintenance energy of Klebsiella aerogenes growing in continuous culture /
[ Djanvan,A.;A.M.James ] / Biotechnol. Lett.
13 Mass and energy balance for microbial growth kinetics /
[ Nagai,S. ] / Adv. Biochem. Eng. Biotechnol.
14 Microcalorimetric study of Escherichia coli aerobic growth: kinetics and experimental enthalpy associated with growth in succinic acid /
[ Dermoun,Z.;J.P.Belaich ] / J. Bacteriol.
15 The use of calorimetry in biotechnology /
[ Von Stockar, U.;I.W.Marison ] / Adv. Biochem. Eng. Biotechnol.
16 Development of an integrated calorimeter using temperature control signals of a bioreactor and on-line measurement of metabolic heat of a microbial cultivation /
[ Hong,K.P.;W.Hur ] / Korean J. Biotechnol. Bioeng.   과학기술학회마을
17 Development of a large-scale biocalorimeter to monitor and control bioprocesses /
[ Voisard,D.;P.Pugeaud;A.R.Kumar;K.Jenny;K.Jayaraman;I.W.Marison;U.von Stockar ] / Biotechnol. Bioeng.   ScienceOn
18 Specific heat flow rate : an on-line monitor and potential control variable of specific metabolic rate in animal cell culture that combines microcalorimetry with dielectric spectroscopy /
[ Guan,Y.;P.M.Evans;R.B.Kemp ] / Biotechnol. Bioeng.   ScienceOn