• 제목/요약/키워드: Replica technique

검색결과 133건 처리시간 0.026초

Fabrication of Multicomponent Protein Microarrays with Microfluidic Devices of Poly(dimethylsiloxane)

  • Jeon, Se-Hoon;Kim, Ui-Seong;Jeon, Won-Jin;Shin, Chee-Burm;Hong, Su-Rin;Choi, In-Hee;Lee, Su-Seung;Yi, Jong-Heop
    • Macromolecular Research
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    • 제17권3호
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    • pp.192-196
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    • 2009
  • Recently, the multi-screening of target materials has been made possible by the development of the surface plasmon resonance (SPR) imaging method. To adapt this method to biochemical analysis, the multi-patterning technology of protein microarrays is required. Among the different methods of fabricating protein microarrays, the microfluidic platform was selected due to its various advantages over other techniques. Microfluidic devices were designed and fabricated with polydimethylsiloxane (PDMS) by the replica molding method. These devices were designed to operate using only capillary force, without the need for additional flow control equipment. With these devices, multiple protein-patterned sensor surfaces were made, to support the two-dimensional detection of various protein-protein interactions with SPR. The fabrication technique of protein microarrays can be applied not only to SPR imaging, but also to other biochemical analyses.

왁스 밀링 방법 및 디지털 광 프로젝션 방법으로 제작된 3본 금속 구조물의 적합도 비교 (Comparison the fit of three-unit metal framework fabricated by wax milling method and digital light projection method)

  • 이정환;안재석
    • 대한치과기공학회지
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    • 제41권1호
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    • pp.9-19
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    • 2019
  • Purpose: This study was conducted to comparative evaluate the marginal and internal gap of three-unit metal frameworks(Co-Cr) fabricated by wax milling method and digital light projection method of CAD/CAM systems. Methods: All the specimens were fabricated by three different fabrication methods: conventional wax up with casting(CWC), milled wax block with casting(MWC), digital light projection with casting(DLPC) (n=10 each). The marginal and internal fits of specimens were examined using a replica technique. The light-body silicone thickness was measured at 8 reference points(each abutment: 16 measurements). All measurements were conducted by a stereomicroscope. Digital photo were taken at $150{\times}$ magnification and then analyzed using a measurement software. The Mann-Whitney test was used for analyzing the results. Results: Statistically significant differences were found between the fabrication methods(p<0.001). The mean(SD) is ${\mu}m$ for fabrication methods, the mean marginal fit were recorded respectively, CWC 63(38), MWC group 50(33), DLPC 103(54) and the mean internal fit CWC 96(47), MWC group 116(41), DLPC 138(66). Conclusion : The marginal and internal fit were statistically different according to the fabrication methods(p<0.001). In all fabrication methods, the greatest misfit was found the occlusal area of all specimens.

SiC 입자 강화 알루미늄기 복합재료의 표면미소 피로균열 발생 및 초기진전거동 (Initiation and Growth Behavior of Small Surface Fatigue Crack on SiC Particle Reinforced Aluminum Composite)

  • 이상협;최영근;김상태;이문환
    • Composites Research
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    • 제21권6호
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    • pp.15-22
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    • 2008
  • 본 연구는 SiC입자를 20% 강화된 알루미늄기 복합재료를 이용해서 평면굽힘피로시험을 향했다. 표면미소피로균열의 발생 및 진전거동은 레프리카법으로 연속관찰을 했고 파괴원인과 파괴기구를 규명하기 위해서 주사전자현미경을 이용했다. da/dn-$K_{max}$ 관계에서 저응력 레벨에서는 여러 개의 균열이 진전하고, 합체 등이 일어나는 것으로부터 진전속도는 꽤 분산(흐트러짐)이 심하고, 고응력 레벨에서는 비교적 흐트러짐은 적게 나타나는 것을 알 수 있었다.

SiC 강화 알루미늄기 복합재료의 표면미소 피로균열 발생 및 진전 거동 (Initiation and Growth Behavior of Small Surface Fatigue Crack in SiC Reinforced Aluminum Composite)

  • 이상협;최영근;김상태
    • Composites Research
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    • 제22권3호
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    • pp.74-81
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    • 2009
  • 본 연구는 SiC 입자 강화된 알루미늄기 복합재료와 SiC 휘스커 강화된 알루미늄기 복합재료를 이용해서 평면굽힘피로시험을 행했다. 표면미소피로균열의 발생 및 진전거동은 레프리카법으로 연속관찰을 했고 파괴원인과 파괴기구를 규명하기 위해서 주사전자현미경을 이용했다. 두 재료 모두 da/dn - $K_{max}$ 관계에서 고응력 레벨에서는 $K_{max}$의 증가에 따라 da/dn이 증가 했지만, 저응력 레벨에서는 da/dn이 감소하다가 증가하는 것을 알 수 있다.

쾌속 조형 기술을 이용하여 제작된 3본 고정성 보철물의 적합도 평가 (Marginal fit evaluation of 3 unit fixed dental prostheses fabricated by rapid prototyping method)

  • 김기백;김재홍;김웅철;김혜영;김지환
    • 대한치과기공학회지
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    • 제34권2호
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    • pp.105-111
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    • 2012
  • Purpose: The purpose this study was to evaluate the marginal fitness of metal copings fabricated using rapid prototyping method and to compare the fitness with copings by conventional method. Then clinical availability of fixed dental prostheses (FDPs) fabricated using rapid prototyping method shall be confirmed based on marginal fitness. Methods: Ten same cases of3 unit FDPs epoxy models (abutment teeth 14 and 16) were manufactured. Each of ten epoxy models were scanned by 3shape D-700 scanner to be designed by experienced technician, and photopolymer 3 unit FDPs were fabricated using rapid prototyping methodand fabricated using Lost wax technique (LW) with same models. Marginal fitness of 3 unit FDPs were measured by silicone replica technique. T-test of independent sample for statistical analysis was executed with SPSS 12.0K for Windows. (${\alpha}$=.05) Results: Significantly higher mean (SD) marginal fitness (P<.000) were observed in the RP group $(95.9(18.0){\mu}m$ compared to the conventionally LW group $(80.1(13.8){\mu}m$. Conclusion: Marginal fitness of LW group showed excellent rather than RP group. However marginal fitness of 3 unit FDPs fabricated by RP method did not get out of clinical allowance value range significantly to allow clinical application.

디지털 미디어 시대의 방송 분장 변화에 관한 연구 (A Study for the Broadcasting Makeup and Image Representation Changes in the Digital Media Era)

  • 방기정;김경희;김주덕
    • 복식문화연구
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    • 제18권6호
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    • pp.1194-1210
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    • 2010
  • The influence of digital media according to environmental change of multi-media came to have significance more than what we imagine. In accordance with high resolution of HDTV in digital media era, the cautious awareness is required for skin color by the immediate color such as replica of TV color, lighting and clothing. As for the broadcasting makeup expression technique caused by a change in broadcasting environment in the digital media era, the first, There is necessity for natural makeup technique, and for expressing the whole makeup evenly and very delicately. The makeup work gets much more delicate. For the delicate expression, more time is being required than the existing makeup time. Second, Lots of time and manpower are required for elaborate real-object processing on all the production fields such as background set, stage properties, and makeup. Third, Realistic expression is available on the screen. Importance of basic makeup is highlighted. Thus, even the skin care shop came to be prevalent. Development in only HD cosmetics is needed for foundation with fine particle in new material and with diverse colors hereafter. The video-media field is a method that is ignored a sense of distance through vehicles such as camera, picture tube, and several kinds of broadcasting machinery and equipment and that is delivered vividly to viewers through screen, unlike the stage makeup, thereby being needed the makeup technology proper for HDTV according to the changing broadcasting environment and media. The video machinery and equipment are proceeding with being gradually high-tech and precise. Thus, an expert in makeup needs to know common sense on the video machinery and equipment before makeup, and needs to make an effort according to it. And, a follow-up research can be said to be necessary on the advance in makeup method and on more diverse dedicated cosmetics along with a research on color tone proper for HDTV.

Comparison of prosthetic models produced by traditional and additive manufacturing methods

  • Park, Jin-Young;Kim, Hae-Young;Kim, Ji-Hwan;Kim, Jae-Hong;Kim, Woong-Chul
    • The Journal of Advanced Prosthodontics
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    • 제7권4호
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    • pp.294-302
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    • 2015
  • PURPOSE. The purpose of this study was to verify the clinical-feasibility of additive manufacturing by comparing the accuracy of four different manufacturing methods for metal coping: the conventional lost wax technique (CLWT); subtractive methods with wax blank milling (WBM); and two additive methods, multi jet modeling (MJM), and micro-stereolithography (Micro-SLA). MATERIALS AND METHODS. Thirty study models were created using an acrylic model with the maxillary upper right canine, first premolar, and first molar teeth. Based on the scan files from a non-contact blue light scanner (Identica; Medit Co. Ltd., Seoul, Korea), thirty cores were produced using the WBM, MJM, and Micro-SLA methods, respectively, and another thirty frameworks were produced using the CLWT method. To measure the marginal and internal gap, the silicone replica method was adopted, and the silicone images obtained were evaluated using a digital microscope (KH-7700; Hirox, Tokyo, Japan) at 140X magnification. Analyses were performed using two-way analysis of variance (ANOVA) and Tukey post hoc test (${\alpha}=.05$). RESULTS. The mean marginal gaps and internal gaps showed significant differences according to tooth type (P<.001 and P<.001, respectively) and manufacturing method (P<.037 and P<.001, respectively). Micro-SLA did not show any significant difference from CLWT regarding mean marginal gap compared to the WBM and MJM methods. CONCLUSION. The mean values of gaps resulting from the four different manufacturing methods were within a clinically allowable range, and, thus, the clinical use of additive manufacturing methods is acceptable as an alternative to the traditional lost wax-technique and subtractive manufacturing.

Gap comparison between single crown and three-unit bridge zirconia substructures

  • Anunmana, Chuchai;Charoenchitt, Masnisa;Asvanund, Chanavut
    • The Journal of Advanced Prosthodontics
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    • 제6권4호
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    • pp.253-258
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    • 2014
  • PURPOSE. To compare marginal and internal gaps of zirconia substructure of single crowns with those of three-unit fixed dental prostheses. MATERIALS AND METHODS. Standardized Co-Cr alloy simulated second premolar and second molar abutments were fabricated and subsequently duplicated into type-III dental stone for working casts. After that, all zirconia substructures were made using $Lava^{TM}$ system. Marginal and internal gaps were measured in 2 planes (mesial-distal plane and buccal-palatal plane) at 5 locations: marginal opening (MO), chamfer area (CA), axial wall (AW), cusp tip (CT) and mid-occlusal (OA) using Replica technique. RESULTS. There were significant differences between gaps at all locations. The $mean{\pm}SD$ of marginal gap in premolar was $43.6{\pm}0.4{\mu}m$ and $46.5{\pm}0.5{\mu}m$ for single crown and 3-unit bridge substructure respectively. For molar substructure the $mean{\pm}SD$ of marginal gap was $48.5{\pm}0.4{\mu}m$ and $52.6{\pm}0.4{\mu}m$ for single crown and 3-unit bridge respectively. The largest gaps were found at the occlusal area, which was $150.5{\pm}0.5{\mu}m$ and $154.5{\pm}0.4{\mu}m$ for single and 3-unit bridge premolar substructures respectively and $146.5{\pm}0.4{\mu}m$ and $211.5{\pm}0.4{\mu}m$ for single and 3-unit bridge molar substructure respectively. CONCLUSION. Independent-samples t-test showed significant differences of gap in zirconia substructure between single crowns and three-unit bridge (P<.001). Therefore, the span length has the effect on the fit of zirconia substructure that is fabricated using CAD/CAM technique especially at the occlusal area.

고정성 보철물의 내면 및 변연적합도를 평가하는 방법에 대한 고찰 (Study on methodology for the assessment of internal and marginal adaptation on fixed dental prosthesis)

  • 표세욱;이준재;한중석;임영준
    • 구강회복응용과학지
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    • 제32권3호
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    • pp.158-168
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    • 2016
  • 본 논문은 기존에 선행되었던 연구들을 바탕으로 고정성 보철물의 내면 및 변연적합도를 평가하는 여러 가지 방법에 관하여 정리하였으며, 각 방법에 대한 장, 단점에 대해 언급하였다. 보철물의 적합도를 평가하는 방법은 크게 임상적 평가법과 실험실 평가법으로 분류할 수 있다. 임상적 평가법에는 탐침 검사법, 방사선 사진 검사법, 인상채득법이 있으며, 실험실 평가법에는 현미경을 이용하는 변연부 측정법, 절단면 측정법, 실리콘 복제 측정법, 그리고 마이크로 CT나 조면계를 사용하여 측정하는 방법 등이 있다. 최근 스캐닝 기법이 발전하면서 내면 및 변연적합도를 3차원적으로 평가하는 것이 가능해졌으며, 앞으로 측정법 및 분석법의 개발에 따라 보철물의 적합도 평가가 더욱 간편해지고 효과적으로 변화할 것이라 생각된다.

치과용 캐드캠을 이용하여 연질금속 재료로 제작한 고정성 보철물의 변연 간격 평가 (Evaluation of Marginal Gap of Fixed Dental Prostheses Fabricated by Soft Metal Material Using Dental CAD/CAM)

  • 김재홍;김원수;김기백
    • 치위생과학회지
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    • 제14권3호
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    • pp.349-355
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    • 2014
  • 본 연구는 최근 치의학계 전반적으로 치과용 캐드캠 시스템의 사용이 증가함에 따라 새로운 재료의 개발도 활발히 이루어지고 있는 현실을 반영하였다. 최근 새롭게 개발된 고정성 보철물의 제작 재료인 연질금속재료를 이용하여 제작된 고정성 보철물의 변연 간격을 평가하여 봄으로써 신재료의 임상적 허용 가능성을 변연 간격을 기준으로 가늠하여보고자 하였으며, 나아가서는 치과의사, 치과위생사 및 치과기공사 등 해당 분야 임상가들에게 참고자료를 제시하고자 하였다. 연구 결과에 따르면 연질금속블록을 가공하여 제작된 집단이 전통적인 방식에 의해 제작된 집단보다 우수한 변연간격을 보였으나, 통계적으로 유의하지 않았다. 또한 연질금속블록을 가공하여 제작된 보철물들의 변연 간격이 임상적 허용 수치로 제시되고 있는 $120{\mu}m$를 넘지 않는 것으로 보아 변연 간격을 기준으로 하였을 때 임상적으로 허용이 가능할 것으로 생각된다.