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A Study on Dose-Response Models for Foodborne Disease Pathogens (주요 식중독 원인 미생물들에 대한 용량-반응 모델 연구)

  • Park, Myoung Su;Cho, June Ill;Lee, Soon Ho;Bahk, Gyung Jin
    • Journal of Food Hygiene and Safety
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    • v.29 no.4
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    • pp.299-304
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    • 2014
  • The dose-response models are important for the quantitative microbiological risk assessment (QMRA) because they would enable prediction of infection risk to humans from foodborne pathogens. In this study, we performed a comprehensive literature review and meta-analysis to better quantify this association. The meta-analysis applied a final selection of 193 published papers for total 43 species foodborne disease pathogens (bacteria 26, virus 9, and parasite 8 species) which were identified and classified based on the dose-response models related to QMRA studies from PubMed, ScienceDirect database and internet websites during 1980-2012. The main search keywords used the combination "food", "foodborne disease pathogen", "dose-response model", and "quantitative microbiological risk assessment". The appropriate dose-response models for Campylobacter jejuni, pathogenic E. coli O157:H7 (EHEC / EPEC / ETEC), Listeria monocytogenes, Salmonella spp., Shigella spp., Staphylococcus aureus, Vibrio parahaemolyticus, Vibrio cholera, Rota virus, and Cryptosporidium pavum were beta-poisson (${\alpha}=0.15$, ${\beta}=7.59$, fi = 0.72), beta-poisson (${\alpha}=0.49$, ${\beta}=1.81{\times}10^5$, fi = 0.67) / beta-poisson (${\alpha}=0.22$, ${\beta}=8.70{\times}10^3$, fi = 0.40) / beta-poisson (${\alpha}=0.18$, ${\beta}=8.60{\times}10^7$, fi = 0.60), exponential (r=$1.18{\times}10^{-10}$, fi = 0.14), beta-poisson (${\alpha}=0.11$, ${\beta}=6,097$, fi = 0.09), beta-poisson (${\alpha}=0.21$, ${\beta}=1,120$, fi = 0.15), exponential ($r=7.64{\times}10^{-8}$, fi = 1.00), betapoisson (${\alpha}=0.17$, ${\beta}=1.18{\times}10^5$, fi = 1.00), beta-poisson (${\alpha}=0.25$, ${\beta}=16.2$, fi = 0.57), exponential ($r=1.73{\times}10{-2}$, fi = 1.00), and exponential ($r=1.73{\times}10^{-2}$, fi = 0.17), respectively. Therefore, these results provide the preliminary data necessary for the development of foodborne pathogens QMRA.

Molecular Cloning and Analysis of Phosphate Specific Transport (pst) Operon from Serratia marcescens KCTC 2172 (Serratia marcescens KCTC 2172로부터 pst operon의 클로닝 및 해석)

  • Lee, Seung-Jin;Lee, Yong-Seok;Lee, Sang-Cheol;Park, In-Hye;Ahn, Soon-Cheol;Choi, Yong-Lark
    • Journal of Life Science
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    • v.19 no.5
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    • pp.566-572
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    • 2009
  • A recombinant plasmid, pDH3, was obtained from the genomic library of Serattia marcescens KCTC 2172, and several recombinant subclones constructed from pDH3. The nucleotide sequence of a 5,137 bp segment, pPH4, was determined and three open reading frames were detected. The three ORFs encoded the phosphate specific transport (pst) operon, which was pstC, pstA, and pstB, with the same direction of transcription. Comparison of the pst operon of S. marcescens with that of other organisms revealed that the genes for pstS and phoU were missing. A potential CRP bonding site and pho box sequence was found in the upstream of the putative promoter at the regulatory region. Analysis of the nucleotide sequence showed that homology in amino acid sequences between the PstC protein and Yersinia sp., Vibrio sp., and Pseudomonas sp. were 49, 37 and 33%, respectively. The PstA protein and Yersinia sp., Vibrio sp., and Pseudomonas sp. showed homologies of 64, 51, and 47%, respectively. PstB protein and Methanocaldococcus sp., E. coli, and Mycoplasma sp. showed homologies of 60, 50, and 48%, respectively. The pst genes could be expressed in vivo and positively regulated by cAMP-CRP. The E. coli strain harboring plasmid pPH7, with pst genes, increased with the transport of phosphate.

A Study on the life of Yoon Cho Chang(尹草窓) and Cho-Chang-Kyeul(草窓訣) (윤초창(尹草窓)의 생애(生涯)와 초창결(草窓訣)에 관(關) 연구(硏究))

  • Kim, Joon Tae;Yoon, Chang Yeul
    • Journal of Korean Medical classics
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    • v.6
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    • pp.189-227
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    • 1993
  • I have studied life of Yoon-Dong-Li(尹東里), who applied Un-Ki(運氣) theory to medicine during Suk-Jong(肅宗) to Joong-Jo(正祖) in Yi-Dynasty and also studied his existing writings named ${\ll}$Cho-Chang-kyeol${\gg}$ (草窓訣). The referenced original work prints were based on 1980's print of Chung-Ku oriental medical society and National Central Library and The academy a korean studies possessed print. Studied with these books, I have concluded as follows. 1. Yoon-Dong-Li(尹東里) courtesy name is Ja-Mi(子美), pen name is Cho-Chang(草窓). He was born in 1705, Suk-Jong(肅宗) 31yrs, and died in 1784, Jeong-Jo(正祖) 8yrs in Yi-Dynasty. He had lineage of third-generationed-doctor and learned medicine from his uncle, Yoon-Woo-Kyo(尹雨敎). His father Yoon-Yi-Kyo(尹이敎) gaved him second influence. 2. Confucianal physician in Ming Dynasty You-Bu(劉溥) respected Ju-Ryeom-Kye(周溓溪), confucianist in Song-Dynasty and You-Bu(劉溥) does not removed grass in front of window (because in chinese, Cho-Charig(草窓) means grass in front of window), and named himself Cho-Chang(草窓). Yoon-Dong-Li(尹東里) followed this suit so called himself Cho-Chang (草窓). 3. The main contents of ${\ll}$Cho-Chang-Kyeol${\gg}$ (草窓訣), (運氣衍論) was written in 1725, when Yoon-Dong-Li(尹東里) was 21yrs old and printed in 1736. The other part of ${\ll}$Cho-Chang-Kyeol${\gg}$ (草窓訣), (用藥篇) was written in 1746. These two parts were not written in the satre time. The (運氣衍論) was written llyears earlier than (用藥). Two parts were combined another day and named (草窓訣). 4. Existing ${\ll}$Cho-Chang-Kyeol${\gg}$ (草窓訣) is transcribed printing with the con tent of "Five elements motion and the six kind of natural factors theory" (五運六氣設). Each edition is generally similar in content, but also has different points each other so naw we hardly finds a complete set of works. 5. ${\ll}$Cho-Chang-Kyeol${\gg}$ (草窓訣) is a first technical book which treats of Un-Ki(運氣) theory in korea. 6. The contents of (運氣衍論) of ${\ll}$Cho-Chang-Kyeol${\gg}$ (草窓訣) is mechanism of diease according to excess and insuffciency of five elements motion and six kinds of nalural faclors.and symploms and priscriptions, includes variated priscriptions. 7. Two parts in (運氣衍論) of ${\ll}$Cho-Chang-Kyeol${\gg}$ (草窓訣), 'Sang-tong' (相通) and 'Kak-Tong' (各通), threats of mochanisim of the disease according to five elements. with the principle of inter-promoting and inter-acting, in the change of ten heavenly stems and five elements motion. 8. In the (用藥篇) of ${\ll}$Cho-Chang-Kyeol${\gg}$ (草窓訣) describes priscription for clinical application according to Un-Ki(運氣) and also records about misuse of medicine. 9. In the ${\ll}$Cho-Chang-Kyeol${\gg}$ (草窓訣), most of priscription are common using one, which also found in ${\ll}$Dong-$\breve{U}$i-Bo-Garm${\gg}$ (東醫寶鑑) and there are few of priscriptions hy Yoon-Dong-Li(尹東里).

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A Review of Combination Effects and Adverse Effects of Yerba Mate (Ilex paraguariensis) on the Treatment of Obesity (비만치료를 목적으로 마테(Yerba Mate, Ilex paraguariensis)를 함유한 복합투여물의 효과 및 부작용에 대한 고찰)

  • Ahn, Jae Hyun;Lee, Min Ho;Lee, Seung Hoon;Choi, Do Young;Lee, Jae Dong
    • Journal of Acupuncture Research
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    • v.33 no.2
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    • pp.135-150
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    • 2016
  • Objectives : The aim of this study is to review the combination effects and adverse effects of Yerba Mate (Ilex paraguariensis) on the treatment of obesity. Methods : Four databases were searched, including Pubmed, National assembly library, RISS4u, and NDSL, for studies published before March 31, 2016 using the keywords 'yerba mate', 'Ilex paraguariensis', and those related to 'obesity'. Clinical trials, and both in vivo and in vitro studies were classified according to the following categories: publication year, nationality, types and composition of test compounds, combination effects, adverse effects, and safety. Results : A total of 16 articles (14 clinical trials, one in vivo, one in vitro) were included in the analysis. Combination effects were reported from six YGD (Yerba Mate, Guarana, Damiana, Zotrim), five Meltdown, one Shred-matrix, one DBX (DymaBurn Xtreme), one XSIII research study, and adverse effects were reported from two YGD (Zotrim), four Meltdown, one Shred-matrix and one MetEF research study. YGD and Shred-matrix increased satiety while YGD and MetEF had adverse gastrointestinal effects potentially due to guarana. Meltdown and Shred-matrix increased energy expenditure and fat oxidation and had the adverse effects of increased heart rate and blood pressure due to the thermogenic effect of caffeine and yohimbine. DBX increased resting energy expenditure but no difference was observed in respiratory exchange ratio. XSIII reduced body weight gain and food intake more than each of the extracts did on their own. Conclusion : Taken together, the combination effects and adverse effects differed according to the composition of test compounds and the ratio of ingredients. Multicomponent approaches should be used for treating multifactorial diseases such as obesity. As a general conclusion, there is a place for a combination of herbal components including yerba mate, based on the principle of Kun-Shin-Choa-Sa for the treatment of obesity.

A Review Study on Adjustment of College Freshmen for Suggesting Adaptation Program (적응프로그램 제언을 위한 대학신입생 학교적응 관련 문헌분석)

  • Yoo, Kyung Hee;Kim, Jong Kyung
    • The Journal of the Korea Contents Association
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    • v.16 no.8
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    • pp.580-591
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    • 2016
  • The purpose of this study was to review articles and theses on adjustment of college freshmen from domestic researches conducted during the past 11 years, to identify overall research trends, and to suggest direction and strategies for college freshman's adjustment. The final 24 articles were selected from three databases, RISS, KISS, and NAL (National assembly library) using specific inclusion criteria. Twenty were correlation studies and four were experimental studies. In 2009, the first study on adjustment of college freshmen was published. Since then several more studies have been conducted to date. This means that people have recognized the importance of adjustment of college freshmen. Twenty three studies that are reviewed used Baker and Siryk's college adjustment tool. From reviewed correlation studies, mean score of college students' adjustment was 3.25. The related variables with adjustment of college freshmen revealed 20 items, especially self-esteem, self-efficacy, and stress showed important related concepts. The general characteristics related to adjustment of freshmen were outgoing character, living with family, aged students, satisfied with the area of study, and satisfied with school life. The findings of this study indicated that colleges when planning for the freshmen orientation program should focus on improving self-efficacy of students, stress management, mentor program, and freshmen adjustment program for each department of the school and college.

Analyses of Studies on Exercise Therapy for Middle-Age Women with Chronic Low Back Pain in Korea (만성요통을 가진 중년 여성의 운동요법에 대한 국내 연구논문 분석)

  • Kwak, Hyeweon;Kim, Nahyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.6
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    • pp.389-399
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    • 2016
  • This study examined the status of studies on exercise interventions for middle-aged women with chronic low back pain that had been conducted over the past 10 years (2005-2014) in Korea. The existing reports were searched electronically using the database of RISS, National Assembly Library, KISS, and DBpia with the key words of middle-aged women, chronic low back pain, exercise, and exercise program. Finally, 12 articles were included in the review. One article was from nursing science, and 11 were from other disciplines. In a qualitative evaluation on the papers, 1 study scored 8 out of 10 points, 8 studies scored between 5-6 points, and 3 studies scored 3-4 points. Intervention sessions were conducted for 55 minutes, on average, each at a frequency of 3.1 sessions per week, for a total of 29.7 sessions. This study found that lumbar neuromuscular exercise, yoga exercise, and aquatic exercise were effective in rehabilitation in middle-aged women with chronic low back pain. In the future, these findings are expected to be used in nursing intervention for the establishment of the basis for evidence-based nursing practice.

Synthesis and Photoluminescence Properties of Y1-x(P1-y-zNbyVz)O4:Eux Phosphors by Modified Combinatorial Chemistry Method (조합화학 기법을 이용한 Y1-x(P1-y-zNbyVz)O4:Eux 형광체의 합성 및 빛 발광 특성)

  • Zeon, Il-Woon;Sohn, Kee-Sun;Park, Hee-Dong
    • Journal of the Korean Chemical Society
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    • v.46 no.1
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    • pp.69-75
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    • 2002
  • The $Y_{1-x}(P_{1-y-z}Nb_yV_z)O_4:Eu_x$ blue and red emitting phosphors were prepared by the combinatorial chemistry method. The combinatorial library was designed to investigate the luminescence of the $Y_{1-x}(P_{1-y-z}Nb_yV_z)O_4:Eu_x$ phosphors under 254 nm and 147 nm excitations. In addition, the crystallinity and morphology of phosphors were checked by XRD and SEM. Based on the results from the combinatorial screenings, luminescent properties of phosphors are strongly dependent on the concentration of doping metal ions. It was found that a new phosphor $Y_{0.88}(P_{0.92}Nb_{0.05}V_{0.03})O_4:Eu_{0.12}$ shows excellent luminescent efficiency comparing to the $Y_{0.88}PO_4:Eu_{0.12}$ red phosphor.

A Systematic Review on the Present Condition of the Internal Robot Therapy (국내 로봇치료 연구 현황에 대한 체계적 고찰)

  • Song, Ji-Hyeon;Sim, Eun-Ji;Yom, Ji-Yun;Oh, Min-Kyeong;Yi, Hu-Shin;Yoo, Doo-Han
    • The Journal of Korean society of community based occupational therapy
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    • v.6 no.1
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    • pp.49-60
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    • 2016
  • Objective : By organizing systematically the study case that use Robot Therapy as intervention tool according to PICO (Patient, Intervention, Comparison, Outcome), This study aims to investigate the domestic Robot Therapy's present condition. Methods : We searched 710 pieces of domestic scientific journal and master's thesis during the past nine years in 'Research Information Sharing Service' and 'National Digital Science Library' database using the keyword 'Robot therapy'. We finally chose 15 pieces of domestic scientific journal and master's thesis among the domestic studies that based on the full text which is affordable and used robot by therapeutic intervention tool. Chosen studies were layed out by PICO that could organize the resources systematically. Results : The quality of study tool was used to the method of evidence-based study level of 5 step classification. More than three stages of quality level study was 13. Result of dividing the studies using robot therapy by intervention field, language, lower extremity(gait), cognition, development and study for the region of the upper extremity of five is advancing. Conclusion : Nationally, the robot therapy has been used in various area that include the upper extremity and lower extremity's intervention of language, cognition, growth and others. We hope that this study for baseline data will be utilized in various area engaging to domestic robot therapy.

Study on the System and Evolution of Type of Men's Costume on the Pattern Books of France in the 19th Century - Focusing on Overcoat and Top-exterior-and-interior(outside) - (19세기 프랑스 패턴북에 나타난 남성복식체계 및 유형변화 - 겉상의의 외투류 및 중간겉상의를 중심으로 -)

  • Kim, Yang-Hee;Ryu, Kyung-Hwa
    • Journal of the Korean Society of Costume
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    • v.66 no.8
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    • pp.157-172
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    • 2016
  • This study aims to present findings of historical design trends of men's fashion, and the characteristics of changes in the types of overcoats and mid jackets shown in French pattern books of the 19th century. The primary data sources for this study comprised one pattern book, each of the17th and 18th century, and 15 pattern books of the 19th century, all of which are conserved by the French National Library. The study methods are as follows: First, analysis of men's fashion trends and the characteristics of changes by type as shown in French pattern books of the 17th to 19th centuries. Second, analysis of the evolution of type of men's overcoats and top-exterior- and-interior (outside) styles shown in French pattern books of the 19th century. As a result, a total of 69 type references were collected from French pattern books of the 17th to 19th centuries. Historically, men's fashion was largely classified into top-exterior, top-exterior-and-interior, top-interior, bottoms and accessories. The two major classifications were as follows: top-exteriors overcoats and robes, And top-exterior-and-interior as top-exterior-and-interior(outside) and top-exterior-and-interior (inside). The study also identified the forms and changes by type. Additionally, this study selected overcoats and top-exterior-and-interior(outside) which fall under typical categories to show the change in the forms of men's fashion of the 19th century. These changes in the types were designated as: continued, faded away, newly appeared and transformed. This study analyzed the time of appearance of the forms by the year of publishing in conjunction with cultural references from previous domestic and international studies. The results showed that the time of changes in the types shown in pattern books did not deviate from the mainstream, which verified their typicality.

Expressional Patterns of Anther-specific Genes from Chinese Cabbage during the Flower Development (배추 약 발달 시기별 유전자의 발현 양상)

  • Kim, Hyun Uk;Chung, Kyu Hwan
    • Horticultural Science & Technology
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    • v.17 no.1
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    • pp.7-10
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    • 1999
  • This study was carried out with the purpose of looking into the transcriptionally regulated genes related to the anther development, characterizing them, and applying their promoters to induce male-sterile plants and restore their fertility. Fifteen anther-specific clones were isolated from the anther cDNA library of Chinese cabbage through the differential screening and sequenced partially at both ends. These partial sequence data showed that cDNA clones BAN52, 84, 101, and 229 are very similar to polygalacturonase, ascorbate oxidase, $H^+-translocating$ ATPase, and pectin esterase genes respectively. However, the other clones have not been matched to any of gene sequences in data bank. In northern dot blot analysis, the transcripts of cDNA clone BAN5, 10, 33, 52, 57, 102, 103, 215, 229 appeared in the flower bud of 2.1 mm in length and their amounts were gradually increased along with the anther development. Transcription of cDNA clone BAN32, 54, 62, 84, 101 began in flower bud of 3.9 mm, which is the late stage in anther development. However, the transcription of BAN87 was very small, but its transcript was detected in all anther developmental stages.

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