• 한국균학회지
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• 제38권2호
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• pp.202-204
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• 2010

멜라닌 생합성에 관련된 tyrosinase 효소활성의 억제를 측정을 위하여, 큰갓버섯(Macrolepiota procera) 균사체 및 균사체 배양액을 이용하여 실험을 하였다. 배양액의 메탄올 분획이 56.6%의 효소활성 억제를 보여주었으며, 농도 의존적억제 실험에서 50%의 tyrosinase 효소활성 억제 농도는 8.78mg/ml이었다. Streptococcus bikiniensis를 이용한 멜라닌생성 억제는 균사체의 메탄올 추출물에서 94.0%의 우수한 결과를 나타내었다(Table 2).

• 생약학회지
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• 제41권2호
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• pp.115-121
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• 2010

The present study was designed to explore an immunostimulating activity of crude extracts of Macrolepiota procera, and a combination therapy of cisplatin and Macrolepiota procera extracts which can potentiate the anti-cancer activity of cisplatin. For these, water extraction of Macrolepiota procera were performed at $4^{\circ}C$(MPE-4) and $100^{\circ}C$(MPE-100). In experimental metastasis of colon26-M3.1 cells, prophylactic intravenous administration of MPE ($80-2,000{\mu}g$/mouse) inhibited tumor metastasis compared with tumor control. Peritoneal macrophages stimulated with MPE produced IL-12 as well as induced tumoricidal activity. In an analysis of NK-cell activity, i.v. administration of MPE ($200{\mu}g$/mouse) significantly augmented NK cytotoxicity to YAC-1 tumor cells. The combination treatments of cisplatin ($20{\mu}g$) and MPE ($100{\mu}g$) exhibited prolongation of lifespan in colon26-M3.1 tumor bearing mouse. These results suggested that MPE stimulate immune system non-specifically and application as adjuvant in cancer treatment.

• Mycobiology
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• 제47권4호
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• pp.368-377
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• 2019

The genus Macrolepiota (Agaricales, Basidiomycota) is easy to recognize at the genus level because of big, fleshy basidiocarps with squamules covering the pileus; a single or double annulus; and big, thick-walled basidiospores with a germ pore. However, morphological identification is often unreliable in Macrolepiota due to similar morphological features among species. Due to the uncertainty of previous morphological identification in the genus Macrolepiota, it is necessary to re-examine Korean Macrolepiota using molecular data. We reexamined 34 Macrolepiota specimens collected from 2012 to 2018 in Korea using a reverse taxonomic approach, whereby species identification was first done based on the internal transcribed spacer (ITS) region analysis, followed by morphological confirmation. We identified the presence of four species: M. detersa, M. mastoidea, M. procera, and M. umbonata sp. nov. Two species (M. detersa and M. mastoidea) were previously unrecorded from Korea and M. umbonata is a new species. Detailed descriptions of all four species and taxonomic key are provided in this study. Macrolepiota procera and M. umbonata are distributed through the country, but M. detersa and M. mastoidea are distributed only in limited areas. According to our results, the combination of ITS locus and morphology proved to be a robust approach to evaluate the taxonomic status of Macrolepiota species in Korea. Additional surveys are needed to verify the species diversity and clarify their geographic distribution.

• 생약학회지
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• 제43권1호
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• pp.32-38
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• 2012

To examine the potentiation of Macrolepiota procera extracts (MPE-4) to act as adjuvant enhancing the tumor specific anti-tumor immune response, tumor vaccine prepared by boiling (HK vaccine) admixed with MPE-4 and immunized in mice. Vaccination of mice with HK vaccine in combination with MPE-4 resulted in higher inhibition in tumor metastasis compared with the mice of HK vaccine alone treatment against live syngeneic tumor cell challenge. The splenocytes from mice immunized HK vaccine mixed with MPE-4 was able to elicit a stronger cytotoxic T lymphocyte (CTL) response as compared with HK vaccine alone. In addition, the splenocytes from MPE-4 admixed HK vaccine immunized mice secreted a higher concentration of Th1 type cytokine such as IFN-${\gamma}$, and GM-CSF. Furthermore, the adoptive transfer of splenocytes from mice immunized HK vaccine and MPE-4 led to a more robust anti-tumour response than the HK vaccine alone. Overall, these results indicate that MPE-4 is a good candidate adjuvant of anti-tumor immune response.

• Mycobiology
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• 제33권1호
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• pp.15-18
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• 2005

Macrolepiota procera, one of edible mushrooms belongs to Agaricaceae of Basidiomycota, has a good taste and good medicinal value. As a preliminary study for the development of artificial cultivation method of edible mushroom, cultural characteristics of M. procera was investigated on various culture media under different environmental conditions. Mycelial growth was compared on culture media composed of various carbon and nitrogen sources, and C/N ratios. The optimal conditions for the mycelial growth were $30^{\circ}C$ and pH 7. M. procera showed the rapid mycelial growth in the PDA media. The optimal carbon and nitrogen sources were maltose and glycine, respectively. The optimum C/N ratio was about 10 : 1 in case that 1% glucose was supplemented to the basal media as carbon source.

• 한국균학회지
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• 제25권4호통권83호
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• pp.253-256
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• 1997

한국산 야생버섯의 독성을 규명하기 위한 연구의 일환으로 46종의 야생버섯의 용혈독성을 검색하여 다음과 같은 결과를 얻었다. 1) 46종 중 Amanita esculenta, A. griseofarinosa, A. longistriata, A. melleiceps, A. phalloides, A. rubescence, A. spissacea, A. vaginata, Hebeloma crustuliniforme, Lepiota cygnea, Macrolepiota procera, 및 Oudemansiella platyphylla 등 12종의 야생 담자균 냉침액이 마우스 적혈구에 대하여 강력한 용혈활성을 나타내었다. 2) A. griseofarinosa 및 H. crustuliniforme은 $100^{\circ}C$ 수용상에서 5분간 가열하여도 그 용혈 활성이 소실되지 않아 열에 매우 안정한 용혈성분을 함유하는 것으로 추정된다.

• 대한의생명과학회지
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• 제8권3호
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• pp.173-177
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• 2002

To investigate the fibrinolytic activities of the Korean basidiomycetes, the Extracts of 50 wild mushrooms were tested for their fibrinolytic activites. Extract from Tricholoma sejunctum showed 175% increased activity to that of plasmin 1.0 U/ml. Marasmius siccus showed 54% of activity, and Laetiporus sulphureus var. miniatus and Macrolepiota procera, 43% and 26% activities, respectively to that of plasmin. But, Cystoderma amianthinum, Lepiota sp., Coprinus sp., Lycoperdon sp. were less than 10% of plasmin activity.

• 한국버섯학회지
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• 제16권2호
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• pp.65-69
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• 2018

주왕산국립공원 내 자생버섯 자원의 서식지별 관리방안을 수립하고 생물다양성, 생태계다양성을 유지하기 위한 기초자료를 제공하기 위해서 2017년 4월부터 10월까지 7개월 동안 버섯조사를 실시하였다. 총 조사된 버섯은 2문, 8강, 21목, 85과 225속, 503분류군이 조사되었다. 분류군별로 보면 주름버섯목이 247분류군으로 48.8%의 분포를 보였으며, 구멍장이버섯목과 무당버섯목이 뒤를 이어 다수 발생하는 종으로 확인되었다. 8월에 가장 많은 종이 출현하였고 해발고도 300-400m 사이에서 가장 많은 분포를 보였는데 이 중 상의지구, 대전사 인근지역과, 거대리 샘골 지역에서 가장 많은 버섯 종류가 조사되었다. 기후변화지표종 중 큰갓버섯과 노루털버섯(능이)이 출현하였다.

• 한국균학회지
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• 제41권2호
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• pp.127-131
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• 2013

본 연구는 한려해상국립공원 인근의 경남지역의 야생버섯에 대한 전통지식을 조사하였다. 자료는 총 53개 마을에서 50~90대까지 145명 주민들의 면담을 통해 수집하였다. 총 19개 야생버섯이 식용버섯으로 사용되었는데 싸리버섯이 식용버섯으로 가장 널리 이용되었고, 큰갓버섯과 표고 순위로 식용하였다. 표고는 식 약용버섯으로 유일하게 사용되었다. 독흰갈대버섯은 독버섯인데 곤충퇴치용 버섯으로 사용하고 있었다. 가장 다양한 버섯이 증언된 지역은 거제시 일운면으로 10종에 이르는 버섯의 전통지식이 확인되었고, 하동군의 진교면에서 8종이 출현하였다. 전통 지식에 대해 조사에 응한 여성이 남성에 비해 2배 이상 많이 증언하였고 연령층은 70~80대가 가장 많았다.

• 한국식품영양학회지
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• 제26권3호
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• pp.375-382
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• 2013

The present study is designed to explore an anti-tumor activity on crude extracts of Oplopanax elatus. Water extractions of Oplopanax elatus were performed at $100^{\circ}C$(OeE-100). OeE-100 doses up to $62.5{\mu}g/m{\ell}$ had no cytotoxicity on the tumor cell lines in vitro. In experimental lung metastasis of colon26-M3.1 carcinoma or B16-BL6 melanoma, the prophylactic intravenous ($4{\sim}100{\mu}g/mouse$) or oral (2 mg/mouse) administration of OeE-100 significantly inhibited tumor metastasis as compared with tumor controls. Peritoneal macrophages stimulated with OeE-100 produced various cytokines such as TNF-${\alpha}$, IL-6 and IL-12. In an analysis of NK-cell activities, i.v. administration of OeE-100 ($10{\sim}100{\mu}g/mouse$) significantly augmented the cytotoxicity to YAC-1 tumor cells. Vaccination of mice with boiling-treated tumor cells (BT-vaccine) in combination with OeE-100 ($100{\mu}g/mouse$) showed higher inhibitions in tumor metastasis when compared with the mice of BT-vaccine treatment. In addition, the splenocytes from OeE-100 admixed BT-vaccine immunized mice secreted a higher concentration of Th1 type cytokine such as IFN-${\gamma}$. These results suggested that the OeE-100 stimulated immune system and was a good candidate adjuvant of anti-tumor immune responses.