DOI QR코드

DOI QR Code

Macrolepiota in Korea: New Records and a New Species

  • Cho, Hae Jin (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Lee, Hyun (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Park, Myung Soo (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Kim, Changmu (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Wisitrassameewong, Komsit (National Center for Genetic Engineering and Biotechnology (BIOTEC)) ;
  • Lupala, Abel (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Park, Ki Hyeong (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Kim, Min Ji (Wood Utilization Division, Forest Products Department, National Institute of Forest Science) ;
  • Fong, Jonathan J. (Science Unit, Lingnan University) ;
  • Lim, Young Woon (School of Biological Sciences and Institute of Microbiology, Seoul National University)
  • Received : 2019.03.10
  • Accepted : 2019.08.23
  • Published : 2019.12.01

Abstract

The genus Macrolepiota (Agaricales, Basidiomycota) is easy to recognize at the genus level because of big, fleshy basidiocarps with squamules covering the pileus; a single or double annulus; and big, thick-walled basidiospores with a germ pore. However, morphological identification is often unreliable in Macrolepiota due to similar morphological features among species. Due to the uncertainty of previous morphological identification in the genus Macrolepiota, it is necessary to re-examine Korean Macrolepiota using molecular data. We reexamined 34 Macrolepiota specimens collected from 2012 to 2018 in Korea using a reverse taxonomic approach, whereby species identification was first done based on the internal transcribed spacer (ITS) region analysis, followed by morphological confirmation. We identified the presence of four species: M. detersa, M. mastoidea, M. procera, and M. umbonata sp. nov. Two species (M. detersa and M. mastoidea) were previously unrecorded from Korea and M. umbonata is a new species. Detailed descriptions of all four species and taxonomic key are provided in this study. Macrolepiota procera and M. umbonata are distributed through the country, but M. detersa and M. mastoidea are distributed only in limited areas. According to our results, the combination of ITS locus and morphology proved to be a robust approach to evaluate the taxonomic status of Macrolepiota species in Korea. Additional surveys are needed to verify the species diversity and clarify their geographic distribution.

Keywords

References

  1. Singer R. The Agaricales in modern taxonomy. 4th ed. Koenigstein: Koeltz Scientific Books; 1986.
  2. Kirk PM, Cannon PF, Minter DW, et al. Dictionary of the fungi. 10th ed. Wallingford: CABI; 2008.
  3. Ge ZW, Zhu LY, Vellinga EC. The genus Macrolepiota (Agaricaceae, Basidiomycota) in China. Fungal Divers. 2010;45(1):81-98. https://doi.org/10.1007/s13225-010-0062-0
  4. Lebel T, Syme A. Sequestrate species of Agaricus and Macrolepiota from Australia: new species and combinations and their position in a calibrated phylogeny. Mycologia. 2012;104(2):496-520. https://doi.org/10.3852/11-092
  5. Otieno DO. Macrolepiota aberdarense, a new edible mushroom from Kenya. Curr Res Environ Appl Mycol J Fungal Bio. 2018;8:247-253. https://doi.org/10.5943/cream/8/2/9
  6. Fazolino EP, Suaza Blandon SC, Alves-Silva G, et al. Taxonomy and phylogeny of Macrolepiota: two new species from Brazil. Mycologia. 2018;110(5):930-940. https://doi.org/10.1080/00275514.2018.1500848
  7. Ge ZW, Chen ZH, Yang ZL. _Macrolepiota subcitrophylla sp. nov., a new species with yellowish lamellae from southwest China. Mycoscience. 2012;53(4):284-289. https://doi.org/10.1007/s10267-011-0167-7
  8. Ding ZQ, Huang SZ. Characteristics and high-yield culture technique of Macrolepiota procea. Edible Fungi. 2003;4:33. Chinese.
  9. Kwon H, Thatithatgoon S. Mushroom growing in Northern Thailand. In: Mushroom Growers' Handbook 1: Oyster Mushroom Cultivation. Seoul, Korea: MushWorld; 2004.
  10. Shim SM, Oh YH, Lee KR, et al. The characteristics of cultural conditions for the mycelial growth of Macrolepiota procera. Mycobiology. 2005;33(1):15-18. https://doi.org/10.4489/MYCO.2005.33.1.015
  11. Bon M, Vallee L, Jacob M. Une nouvelle lepiote toxique: Macrolepiota venenata Bon sp. nov. Documents mycologiques. 1979;9:13-21.
  12. Yokoyama K, Yamaji D. Poisoning by Lepiota neomastoidea. Trans Mycol Soc Jpn. 1981;22:255-258.
  13. Mazzolai I. Intossicazioni da Macrolepiota venenata Jacob ex Bon. Riv Micol. 1989;32:264-265.
  14. Lehmann PF, Khazan U. Mushroom poisoning by Chlorophyllum molybdites in the Midwest United States. Mycopathologia. 1992;118(1):3-13. https://doi.org/10.1007/BF00472564
  15. Kim SY, Baek YH, Han SY, et al. Mushroom poisoning by Macrolepiota neomastoidea. Kor J Gastroenterol. 2018;71(2):94-97. https://doi.org/10.4166/kjg.2018.71.2.94
  16. Candusso M, Lanzoni G. Fungi Europaei 4. Lepiota sl. Saronno: Giovanna Biella; 1990.
  17. Bon M. European flora of large mushrooms 3. Lepiotaceae (translated and edited by F. Medjebeur-Thrun, WU Thrun). IHW-Verlag: Eching; 1996. German.
  18. Jennings DH. Morphological plasticity in fungi. Symp Soc Exp Biol. 1986;40:329-346.
  19. Kues U, Navarro-Gonzalez M. How do Agaricomycetes shape their fruiting bodies? 1. Morphological aspects of development. Fungal Biol Rev. 2015;29(2):63-97. https://doi.org/10.1016/j.fbr.2015.05.001
  20. Johnson J. Phylogenetic relationships within Lepiota sensu lato based on morphological and molecular data. Mycologia. 1999;91(3):443-458. https://doi.org/10.2307/3761345
  21. Vellinga EC, Rogier PJ, Bruns TD. Phylogeny and taxonomy of Macrolepiota (Agaricaceae). Mycologia. 2003;95(3):442-456. https://doi.org/10.2307/3761886
  22. Vellinga EC. Chlorophyllum and Macrolepiota (Agaricaceae) in Australia. Aust Systematic Bot. 2003;16(3):361-370. https://doi.org/10.1071/SB02013
  23. Kaburagi Y. Korea forest experiment station. Korean and Manchurian practical manual of forest. Tokyo: Yokendo; 1940.
  24. Lee YS, Lim YW, Kim JJ, et al. Korean Society of Mycology. National list of species of Korea: Basidiomycota. Incheon: National Institute of Biological Resources; 2015.
  25. Markmann M, Tautz D. Reverse taxonomy: an approach towards determining the diversity of meiobenthic organisms based on ribosomal RNA signature sequences. Philos Trans R Soc Lond, B, Biol Sci. 2005;360(1462):1917-1924. https://doi.org/10.1098/rstb.2005.1723
  26. Rogers SO, Bendich AJ. Extraction of total cellular DNA from plants, algae and fungi In: Gelvin SB, Schilperoort RA, editors. Plant molecular biology manual. Boston (MA): Kluwer Academic Publishers; 1994. p. 183-190.
  27. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol. 1993;2(2):113-118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  28. Park MS, Fong JJ, Lee H, et al. Delimitation of Russula subgenus Amoenula in Korea using three molecular markers. Mycobiology. 2013;41(4):191-201. https://doi.org/10.5941/MYCO.2013.41.4.191
  29. Tamura K, Peterson D, Peterson N, et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28(10):2731-2739. https://doi.org/10.1093/molbev/msr121
  30. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013;30(4):772-780. https://doi.org/10.1093/molbev/mst010
  31. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics. 2014;30(9):1312-1313. https://doi.org/10.1093/bioinformatics/btu033
  32. Miller MA, Pfeiffer W, T S. Creating the CIPRES science gateway for inference of large phylogenetic trees. SC10 workshop on gateway computing environments (GCE10); Nov 13-19; New Orleans (LA): IEEE Computer Society; 2010. p. 1-8.
  33. Stamatakis A. Phylogenetic models of rate heterogeneity: a high performance computing perspective. In Proceedings 20th IEEE International Parallel & Distributed Processing Symposium; 2006. Apr 25-29; Rhodes Island: IEEE; 2006. p. 278.
  34. Breitenbach J, Kranzlin F. Fungi of Switzerland, Vol. 4. Agarics 2nd part. Lucerne: Verlag Mykologia; 1995.
  35. Vellinga EC. Macrolepiota In: Noordeloos ME, Kuyper TW, Vellinga EC, editors. Flora agaricina neerlandica, Vol. 5. Lisse, Abingdon, Exton (PA), Tokyo: A.A. Balkema Publishers; 2001. p. 64-73.
  36. Kornerup A, Wanscher JH. Methuen handbook of colour. 3rd ed. London: Eyre Methuen Ltd.; 1978.
  37. Largent D, Johnson D, Watling R. How to identify fungi to genus III: microscopic features. CA: Mad River Press; 1977.
  38. Scopoli JA. Flora carniolica (in Latin). 2nd ed. Vienna: K.P. Krause; 1772.
  39. Singer R. New and interesting species of Basidiomycetes. II. Paper Michigan Acad Sci. 1948;32:103-150.

Cited by

  1. 말뚝버섯속의 국내 미기록종(Phallus hadriani) 보고 vol.48, pp.3, 2020, https://doi.org/10.4489/kjm.20200034
  2. Description of Lepiotaceous Fungal Species of the Genera Chlorophyllum, Clarkeinda, Macrolepiota, Pseudolepiota, and Xanthagaricus, from Laos and Thailand vol.13, pp.12, 2019, https://doi.org/10.3390/d13120666