• 한국연소학회:학술대회논문집
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• 2003.05a
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• pp.195-199
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• 2003

The stoichiometric methan/air premixed turbulent flames at the axisymmetric Bunsen burner situation are numerically investigated. To account for the chemistry-turbulence interaction in the turbulent premixed flames, the steady laminar flamelet library method has been adopted. The flame front is tracked by using the Level-Set Approach. Turbulence is represented by the ${\kappa}-{\varepsilon}$ modeling with a Pope's correction. The detailed comparison between prediction and measurement has made for the flame field in terms of velocity, turbulent kinetic energy, and normarlized temperature.

• Journal of Microbiology and Biotechnology
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• v.24 no.2
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• pp.217-224
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• 2014

Chicken are considered as the most important source of human infection by Campylobacter jejuni, which primarily arises from contaminated poultry meats. However, the genes expressed in vivo of the interaction between chicken and C. jejuni have not been screened. In this regard, in vivo-induced antigen technology (IVIAT) was applied to identify expressed genes in vivo during interaction between chicken and C. jejuni, a prevalent foodborne pathogen worldwide. Chicken sera were obtained by inoculating C. jejuni NCTC 11168 into Leghorn chickens through oral and intramuscular administration. Pooled chicken sera, adsorbed against in vitro-grown cultures of C. jejuni, were used to screen the inducible expression library of genomic proteins from sequenced C. jejuni NCTC 11168. Finally, 28 unique genes expressed in vivo were successfully identified after secondary and tertiary screenings with IVIAT. The genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, regulation and other processes, in addition to Cj0092, with unknown function. Several potential virulence-associated genes were found to be expressed in vivo, including chuA, flgS, cheA, rplA, and Cj0190c. We selected four genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results indicated that these selected genes were significantly upregulated in vivo but not in vitro. In short, the expressed genes in vivo may act as potential virulence-associated genes, the protein encoded by which may be meaningful vaccine candidate antigens for campylobacteriosis. IVIAT provides an important and efficient strategy for understanding the interaction mechanisms between Campylobacter and hosts.

• Journal of the Korean Society for information Management
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• v.34 no.1
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• pp.73-110
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• 2017

This study explores the effectiveness and applications of event collection programs, which are integrated programs with event experience and collection experience as a cooperation model that combines school education and library resources as course-related materials. For the purpose, a triangulated analysis with both quantitative and qualitative data was conducted. As a result, This study examined an event collection program's effectiveness which were positive effects on the participated students' reading and perceptions on the roles of their school libraries. The event collection supported the students to self-construct their knowledge by exploring information with the provision of a collection with various materials on a topic, which would be hardly provided within the traditional library subject classification, and that the event collection program could be a useful tool for self-directed reading due to its promotion of interaction, sharing, and participation among materials and the participated students by functional factors of experiential marketing.

• Journal of Life Science
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• v.18 no.3
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• pp.298-303
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• 2008

Using a phage peptide library approach, we have isolated a peptide ligand that binds to transferrin receptor on the surface of human melanoma cell, B16F10. The library was first screened twice by recovering internalized phages and was further screened three times by competitively eluting transferrin receptor-specific phages with human transferrin among the phages bound to the cell surface. The peptides displayed by the selected phages were fused to translocation and catalytic domain of Pseudomonas exotoxin to prepare recombinant toxins. After estimating cytotoxicity of each recombinant toxin toward B16F10 cell, seven clones were selected. Sequence analysis revealed that one of the clones displayed a peptide which had a significant sequence homology with human transferrin. The peptide was chemically synthesized and was shown to be functional in delivering cytotoxic agents into B16F10 cell via interaction with transferrin receptor.

• Journal of Microbiology
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• v.41 no.1
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• pp.46-51
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• 2003

RAD6 in yeast mediates postreplication DNA repair and is responsible for DNA-damage induced mutations. RAD6 encodes ubiquitin-conjugating enzyme that is well conserved among eukaryotic organisms. However, the molecular targets and consequences of their ubiquitination by Rad6 have remained elusive. In Aspergillus nidulans, a RAD6 homolog has been isolated and shown to be an allele of uvs). We screened a CDNA library to isolate UVSJ-interacting proteins by the yeast two-hybrid system. JIPA was identified as an interactor of UVSJ. Their interaction was confirmed in vitro by a GST-pull down assay. JIPA was also able to interact with mutant UVSJ proteins, UVSJl and the active site cysteine mutant UVSJ-C88A. The N- and the C-terminal regions of UVSJ required for the interaction with UVSH, a RAD18 homolog of yeast which physically interacts with Rad6, were not necessary for the JIPA and UVSJ interactions. About 1.4 kb jipA transcript was detected in Northern analysis and its amount was not significantly increased in response to DNA-damaging agents. A genomic DNA clone of the jipA gene was isolated from a chromosome I specific genomic library by PCR-sib selection. Sequence determination of genomic and cDNA of jipA revealed an ORF of 893 bp interrupted by 2 introns, encoding a putative polypeptide of 262 amino acids. JIPA has 33% amino acid sequence identity to TIP41 of Saccharomyces cerevisiae which negatively regulates the TOR signaling pathway.

• The Plant Pathology Journal
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• v.35 no.3
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• pp.257-273
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• 2019

Tomato (Solanum lycopersicum) is one of the most widely grown and economically important vegetable crops in the world. Tomato chlorosis virus (ToCV) is one of the recently emerged viruses of tomato distributed worldwide. ToCV-tomato interaction was investigated at the molecular level for determining changes in the expression of tomato genes in response to ToCV infection in this study. A cDNA library enriched with genes induced in response to ToCV infection were constructed and 240 cDNAs were sequenced from this library. The macroarray analysis of 108 cDNAs revealed that the expression of 92 non-redundant tomato genes was induced by 1.5-fold or greater in response to ToCV infection. The majority of ToCV-induced genes identified in this study were associated with a variety of cellular functions including transcription, defense and defense signaling, metabolism, energy, transport facilitation, protein synthesis and fate and cellular biogenesis. Twenty ToCV-induced genes from different functional groups were selected and induction of 19 of these genes in response to ToCV infection was validated by RT-qPCR assay. Finally, the expression of 6 selected genes was analyzed in different stages of ToCV infection from 0 to 45 dpi. While the expression of three of these genes was only induced by ToCV infection, others were induced both by ToCV infection and wounding. The result showed that ToCV induced the basic defense response and activated the defense signaling in tomato plants at different stages of the infection. Functions of these defense related genes and their potential roles in disease development and resistance to ToCV are also discussed.

• Journal of the Korean Society for Marine Environment & Energy
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• v.20 no.2
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• pp.63-75
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• 2017

In offshore environment, an accurate estimation of a wave-structure interaction has been an important issue for safe and cost effective design of fixed and floating offshore structures exposed to a harsh environment. In this study, a wave-structure interaction around a circular column was investigated with regular waves. To simulate 3D two-phase flow, open source computational fluid dynamics libraries, called OpenFOAM, were used. Wave generation and absorption in the wave tank were activated by the relaxation method, which implemented in a source term. To validate the numerical methods, generated Stokes 2nd-order wave profiles were compared with the analytic solution with deep water condition. From the validation test, grid longitudinal and vertical sizes for wave length and amplitude were selected. The simulated wave run-up and wave loads on the circular column were studied and compared with existing experimental data.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.34 no.1
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• pp.97-120
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• 2023

This study determined the communicative characteristics of public libraries through locality, communality, interactivity, and organism and sought to measure the validity of their components to define the role of libraries based on the analysis. According to the analysis results, there are four communicative characteristics of public libraries: locality, communality, interactivity, and organism. This study defined the role of public libraries according to communicative characteristics. First, libraries should encourage the formation of a healthy society by providing a space for diverse community activities, contributing to the building and strengthening of their local communities, and offering the necessary information to do so. Second, libraries should contribute to the formation of local cultural communities and strengthen interpersonal relations among community members to reinforce a sense of community, while also supporting the heightening of social adaptability to foster a sense of belonging and community activity participation among members. Third, libraries act as a space for sharing ideas between generations, provide a space to carry out leisure activities, and enable the cultural prosperity of local communities. Lastly, as a representative space of local communities, libraries raise the value of the residential environment and contribute to their communities and members.

• Journal of the Korean Society for Library and Information Science
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• v.51 no.3
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• pp.109-132
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• 2017

This study analyzed the phenomenological research method of Giorgi in order to understand the changes in the self-esteem of middle-aged women through group bibliotherapy. During this study, 12 sessions were conducted for six middle-aged women, and the participants' statements in the recorded files were analyzed and integrated into 221 semantic units, 16 sub-situations/topics, 6 situations/topics, and 3 groups. During the research, the participants suffered from neurosis symptoms due to family difficulties. Nevertheless, bibliotherapy showed favorable results; the use of picture books and thus the interaction between the participants led to positive, emotional and psychological responses. Furthermore, from the study, it was concluded that religious life played an important role in healing the participants' wounds.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.24 no.2
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• pp.231-246
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• 2013

This paper attempts to explore the use of social metadata in academic libraries. A total of 173 academic libraries were examined and analyzed. Various social metadata were reviewed, involved with users' participation and contribution. Error-reports, tagging, recommendations, ratings, reviews, comments, sharing, and community were identified that support selection, sharing and collaboration through social engagement. Suggestions drawn from the findings are offered to utilize social metadata in order to enhance users' contribution and interaction. It is hoped that this exploratory study will provide insight into the use of social metadata in academic libraries.